1,197 research outputs found
First person – Simona Amodeo
No full textABSTRACT
First Person is a series of interviews with the first authors of a selection of papers published in Journal of Cell Science, helping early-career researchers promote themselves alongside their papers. Simona Amodeo is the first author on ‘Characterization of the novel mitochondrial genome replication factor MiRF172 in Trypanosoma brucei’, published in Journal of Cell Science. Simona is a PhD student in the lab of Torsten Ochsenreiter at the Institute of Cell Biology, University of Bern, Switzerland, investigating mitochondrial genome anchoring, replication and inheritance in Trypanosoma brucei.</jats:p
Microbial bioremediation of aquifer affected by chloroethenes and petroleum hydrocarbon contamination
No full textIn 2014, 20% of contaminated site in Europe were treated by bioremediation techniques, prevalent contaminants being: petroleum hydrocarbons, chlorinated compounds and heavy metals. Mixed contaminations can be lowered by anaerobic (reductive dechlorination) and aerobic (oxidation) microbial metabolisms. Successive anaerobic-aerobic systems can be used for biodegradation of by-products, such as vinyl chloride, and petroleum hydrocarbons. In order to assess feasible treatment to improve reductive dechlorination at a contaminated site (Dogaletto, VE, Italy), anaerobic microcosms were set up by using groundwater samples added or not with molasses. GC-MS analysis demonstrated that the addition of the substrate improved reductive dechlorination reactions, leading to higher concentrations of vinyl chloride and ethenes. The addition of molasses was then applied at the contaminated aquifer in the frame of an anaerobic active permeable barrier treatment. Two year field monitoring evidenced that molasses addition improved dehalogenation of high chlorinated ethenes (i.e. perchloroethene from 900 to 450 μg/l) with concomitant formation of vinyl chloride that increased from 33’00 to 69’000. The treatment affected the microbial community structure: Bacteroidetes, glucose fermenting bacteria, relatively increased in relation to dechlorinating bacteria of the Dehalococcoides genus. Despite this, Dehalococcodes gene copy number increased, as determined by q-PCR. In the aerobic active permeable barrier, vinyl chloride and petroleum hydrocarbons decreased, respectively, from 13’550 to 2’600 μg/l and from 3’171 to 1’368 μg/l. A low number of BTEX-degrading bacteria were isolated and characterized and vinyl chloride degrading populations are under study. The obtained data demonstrated that bioattenuation processes were active at the contaminated aquifer and specialized anaerobic and aerobic bacterial populations can be further exploited for a complete bioremediation
Characterization of PGPR for improving plant resistance under environmental stresses
No full textRhizobacteria having plant growth promoting (PGPR) characteristics amplify plant resistance to biotic and abiotic stresses. Relatively recently, it was discovered that many PGPR containing the enzyme 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase protect plants against environmental stresses such as flooding, metals, organic toxicants, high salt, drought and phytopathogens.
Arsenic pollution has become a severe worldwide problem due the toxicity of its inorganic forms, arsenate and arsenite. Arsenate is the main species of arsenic in aerated soils and can induce toxic effects in plants. Its uptake and toxicity are intimately linked to the phosphorus-status of plants due to the chemical and biochemical analogies of arsenate and phosphate.
Bacteria use general and specific detoxification strategies to withstand the growth restriction when they are exposed to arsenic. While the general systems alleviate arsenic induced cell toxicities, the specific systems are involved in arsenic transformation, sequestration and solubilization.
A group of arsenic resistant rhizobacteria were isolated and identified from uncontaminated and arsenic contaminated sites. Twelve aerobic rhizobacteria showed a diverse arsenate and arsenite resistance level when growing on rich or defined media supplemented with up to 400 mmol/l of arsenate and 25 mmol/l of arsenite. General resistance mechanisms were investigated by studying microbial growth in LB medium under osmotic stress induced by sodium chloride, sodium arsenate, and polyethylene glycol (PEG 6000). The growth characteristics of the most arsenic resistant bacteria were compared in the presence of 200 mmol/l of sodium arsenate or 1200 mmol/l of sodium chloride (with similar ionic strength) and under an osmotic stress (-1.5 M Pa) generated by 175 mmol/l of sodium arsenate, 400 mmol/l of NaCl and 26% PEG 6000 (Sosa et al., 2005). Results showed that growth was generally better under osmotic stress generated by arsenic than under that generated by NaCl or PEG 6000. Among the isolates, all exhibiting some potential plant growth promotion characteristics, eight strains were ACC deaminase positive and three solubilized phosphate. Arsenic specific resistant mechanisms were determined by identifying the functional genes involved in arsenate reduction (ars and arr genes) based on PCR method. Arsenic transformations by rhizobacteria were analyzed in Tris Mineral Medium with low phosphate content (Mergey et al., 1985) supplemented with gluconate (0.6% , w/v) (TMMG) and spiked with 3mM of arsenate or arsenite.
Experimental results suggested that these arsenic resistant rhizobacteria are metabolically adapted to arsenic-induced osmotic stress in addition to the specific system to control the uptake, reduction and extrusion of arsenic. These isolates can potentially be used to remove arsenic from soils and also to increase the phosphorus bioavailability in agricultural soils as well as in arsenic- contaminated soils to improve plants’ phosphorus nutritio
Materiali e discussioni per l'analisi dei testi classici. Indici 1-60
No full textIl volume raccoglie e riassume tutti gli articoli apparsi nei volumi 1-60 della rivista «Materiali e discussioni per l’analisi dei testi classici» («MD»), nel periodo compreso tra il 1978 e il 2008. Esso è diviso in tre parti. Nella prima sono riportati in successione i singoli numeri della rivista, con il relativo sommario. Nella seconda compaiono gli articoli riuniti in sequenza cronologica, sotto il nome dell’Autore e ognuno con un proprio numero d’ordine: di ogni articolo è fornito il sunto, con i concetti, le argomentazioni salienti, i principali luoghi discussi. Nella terza parte sono elencati gli Autori antichi e i Nomi e parole notevoli, con il rinvio al numero d’ordine dell’articolo. Le schedature dei numeri 1-30 sono curate da Andrea Cucchiarelli, quelle dei numeri 31-60 da Simona Fortini.The volume collects and summarizes all the articles published in Volumes 1-60 of the journal "Materiali e discussioni per l’analisi dei testi classici” ("MD"), in the period between 1978 and 2008. It is divided into three parts. In the first part the individual issues of the journal are summarized with the table of contents. In the second part the articles appear in chronological order, under the name of the author and each with its own serial number: each item is provided with a summary including the main points of argumentation and the loci discussed. The third part contains the indexes of the ancient authors, of the names and of the most relevant things and words, with reference to the number of the item. The profiling of the numbers 1-30 is cared for by Andrea Cucchiarelli, those of the numbers 31-60 by Simona Fortini
Boccaccio nel Seicento: censure e recuperi della "compassione"
No full textSIMONA MORANDO, Boccaccio in the sixteenth century: censorship and recovery ofthe «compassion»
In the introduction of this paper, the literature of the seventeenth century is defined as a writing of compassion but not of consolation (quotations from Giovanni Cisano, Torquato Accetto, Emanuele Tesauro, G. B. Marino). The distance between the
writers studied here and Boccaccio is due to the censored editions of the Decameron in sixteenth century, to the censorship of Boccaccio as a «maestro d’amore», to his unrefined language, to his interpretation by many critics as a light and burlesque author. But, as reported in a writing by Boccalini, Boccaccio’s Latin works are very much appreciated in seventeenth century literature. This is especially the case of De casibus virorum illustrium, a gloomy and pessimistic book about misfortunes of famous men and about compassion, which nevertheless believes in the ability of letters to give back fame to ruined men. As shown in the conclusion
through some writings by Tassoni, many other seventeenth century authors do not have this kind of hope
Replicon Dynamics, Dormant Origin Firing, and Terminal Fork Integrity after Double-Strand Break Formation
No full textSummaryIn response to replication stress, the Mec1/ATR and SUMO pathways control stalled- and damaged-fork stability. We investigated the S phase response at forks encountering a broken template (termed the terminal fork). We show that double-strand break (DSB) formation can locally trigger dormant origin firing. Irreversible fork resolution at the break does not impede progression of the other fork in the same replicon (termed the sister fork). The Mre11-Tel1/ATM response acts at terminal forks, preventing accumulation of cruciform DNA intermediates that tether sister chromatids and can undergo nucleolytic processing. We conclude that sister forks can be uncoupled during replication and that, after DSB-induced fork termination, replication is rescued by dormant origin firing or adjacent replicons. We have uncovered a Tel1/ATM- and Mre11-dependent response controlling terminal fork integrity. Our findings have implications for those genome instability syndromes that accumulate DNA breaks during S phase and for forks encountering eroding telomeres
Le chiavi del Paradiso. Primato petrino e devozione mariana di Sisto IV tra Cappella Sistina e S. Maria della Pace
No full textIl volume è strutturato in due parti distinte con una postfazione, un indice dei nomi e n. 55 Tavole a colori fuori testo, valide per entrambe le parti del volume. I. PARTE (di Lorenzo Cappelletti) A partire dalla esplicita indicazione che l’ignoto architetto, collocato sul fianco destro dell’affresco sistino della Consegna delle chiavi di Perugino, fa dell’edificio a pianta centrale che campeggia sullo sfondo, viene operata una completa rilettura iconografica e iconologica dell’intero affresco. Tenendo conto di tutta la migliore bibliografia sul tema e grazie anche alla considerazione di fonti inedite o mai considerate in relazione a tale affresco –– vengono riletti in particolare i due episodi evangelici sullo sfondo, la teoria dei dodici apostoli e dei personaggi dell’attualità di fine Quattrocento e soprattutto il gesto della consegna delle chiavi a Simon Pietro da parte di Gesù.
La rilettura si allarga necessariamente anche alla Punizione di Core, Datan e Abiram, l’affresco di Botticelli posto in chiave tipologica dirimpetto alla Consegna delle chiavi, e alla serie dei 30/32 papi affrescati nel cleristorio.
Lo studio non manca di offrire inoltre spunti per una comprensione più adeguata anche degli altri affreschi quattrocenteschi della sistina. II PARTE (di Simona Benedetti) Nell’ambito della revisione di lettura iconologica e iconografica dell’affresco della Consegna delle chiavi di Perugino nella Cappella Sistina, svolta da Lorenzo Cappelletti, si torna a riflettere anche sul significato dell’architettura dell’edificio rappresentato al centro dell’affresco. In questo senso nel contributo di Simona Benedetti, in primo luogo, si ripercorrono i riferimenti alla trattatistica quattrocentesca in materia di edifici sacri, che possono aver influito nella determinazione dell’edificio ottagonale.
In seconda istanza, si considerano le principali fabbriche a pianta ottagonale costruite precedentemente all’edificio dipinto, che possono aver costituito un fondato riferimento per il Perugino nella Consegna delle chiavi.
In particolare tra le architetture, che possono avere avuto un’influenza determinante nella definizione iconografica della fabbrica ottagonale presente nell'affresco, la chiesa di S. Maria della Pace è quella su cui si concentra il presente studio. L'opera si realizzò nel cuore pulsante della Roma rinascimentale proprio contemporaneamente all’affresco in esame, con esso trova degli innegabili punti di tangenza e corrispondenze, sia storiche che figurative. A questo riguardo interessantissima risulta la connessione tra il motivo dell’edificazione della fabbrica e i fatti storici, i personaggi, gli eventi prodigiosi e le pratiche devozionali, che condizionarono lo svolgersi del cantiere del Templum Pacis negli ultimi anni del pontificato di Sisto IV. La storicità del gesto della consegna delle chiavi a Pietro - avvenimento che si protrae nel tempo ad ogni elezione pontificia nello spazio fisico della Cappella Sistina - costituisce la centralità del messaggio iconografico dell'affresco del Perugino in cui, anche l’architettura, insieme ai personaggi rappresentati, strutturano una narrazione reale, estranea alle simbologie decontestualizzate dall’epoca propriamente sistina. Segue una postfazione di Francesco Andreani dal titolo Enigma del Quattrocento, saggio nel quale si sviluppano considerazioni sulle personalità degli architetti e maestranze attive nella Roma del tardo Quattrocento e negli anni di inizio della fabbrica di S. Maria della Pace. Il volume si conclude con l'indice dei nomi (a cura di Gemma Fusciello)I (of Lorenzo Cappelletti) Starting from the unknown architect’s forefinger pointed to the central plan building found in the background of the Sistine Perugino’s fresco The Keys of the Kingdom of Heaven, the author offers a new interpretation of the entire fresco at both an iconographical and iconological level.
Having considered the most important studies about this fresco and taking into account a number of unpublished and unconsidered sources, the author rereads in particular the meaning of the central plan building, as well as the two Gospel episodes in the background; and he rereads also the grouping of the Twelve, along with the fifteenth century historical personages painted among them, in the foreground.
The author’s vision necessarily broodens also to comprehend Botticelli’s fresco named The Punishment of Corah, Dathan and Abiram which is placed on the opposite wall as a typological prefiguration of The Keys of the Kingdom of Heaven; and it broodens moreover to reconsider the series of 30/32 popes frescoed in the upper register of the Sistine Chapel.
The essay also offers hints for a more complete comprehension of the other fifteenth century frescoes of the Sistine Chapel.
II. (of Simona Benedetti) As part of the review of iconological and iconographic reading about the fresco of the “Delivery of the keys” by Perugino in the Sistine Chapel, conducted by Lorenzo Cappelletti, you go back and think about the significance of the building architecture depicted in the center of the fresco. In this sense, the contribution of Simona Benedetti, first, retraces the references to the fifteenth-century treatises concerning sacred buildings, which may have affected the determination of the octagonal building. Secondly, we consider the main factories octagonal building constructed previously painted, that may have been an established reference for the Perugino in the “Delivery of the keys”. Especially between architectures, which may have had a decisive influence in defining the iconographic factory octagonal present in the fresco, the church of St. Mary of Peace is the one on which this study focuses. The work was realized in the heart of Renaissance Rome just simultaneously fresco concerned, although it is undeniable points of contact and correspondence, both historical and figurative. In this regard are interesting connections between the reason of building the factory, the historical facts, the characters and the miraculous events and devotional, which has conditioned the unfolding of the construction site of the Templum Pacis in the last years of the pontificate of Sixtus IV. The historicity of the act of handing over the keys to Peter, that continues over time in the physical space of the Sistine Chapel, is the centrality of the iconographic message of the fresco by Perugino; so even the architecture, along with the characters represented, the real narrative structure, far from to the symbolism belonging to the Sistine period properly
Playing with autism. Encountering Simona Concaro by her music. Vol. 1.1
No full textPlaying with Autism contains twenty-four original pieces for piano, recorded and carefully transcribed in current music notation by Pierluigi Politi and Hanna Shybayeva. Simona Concaro, the author, is a young woman suffering of low-functioning autism. She lives in Cascina Rossago, a farm-community, designed to meet the needs of people with autism, in Oltrepo pavese (Italy).
Since she was three, Simona Concaro has created original compositions on piano, instrument she learned by herself, refusing any kind of musical education. In everyday life, Simona does not use verbal language and has low autonomy. Her music, on the contrary, contains interesting features – melodic, rhythmic and harmonic –, even in the presence of some iterative elements, typical of the autistic spectrum
Counseling Youth Online
No full textOnline counseling is becoming more pervasive; this type of guidance method is occurring, and it will likely in-crease within youth work. Studying the phenomena can give relevant resources to the field of digital youth work. The main purpose of this thesis is to research how to provide quality online counseling for youth and to write guidelines for professionals and volunteer workers.
As a facilitator the author researches the themes related to online counseling and enhances discussion through four online group discussions organized for youth workers working in online chats. The aims of the thesis are to find out with qualitative methods how professionals deliver efficiently and effectively online counseling sessions. On a wider perspective, the thesis tries to trace guidelines for counseling young people online, providing em-powerment, direction, guidance and support.
Online counseling has been recently widely studied due to the spread of these kind of services. Due to the uniqueness of digital youth work in Finland, its context and its principles within online counseling and guidance, it is relevant to gain more knowledge about practices and professionals’ perspective
Eco-tales by Simona Kossak
No full textThis article discusses the life and work of Simona Kossak, a writer and professor of forestry. Her life work aimed at awakening in her readers and listeners sensitivity and compassion towards animals and natural surroundings. The author examines Kossak’s radio talks which she proposes to name “eco-tales”[email protected] Trusewicz, absolwentka filologii polskiej na Uniwersytecie w Białymstoku, doktorantka w Zakładzie Teorii i Antropologii Literatury w Instytucie Filologii Polskiej UwB. Interesuje się geopoetyką, ekokrytyką oraz obrazami Puszczy Białowieskiej w literaturze i kulturze. W kręgu jej zainteresowań badawczych mieszczą się również tematy związane z literaturą regionu. Współorganizatorka dwóch doktoranckich konferencji naukowych na Uniwersytecie w Białymstoku, wpisujących się w rozważania dotyczące wyobraźni przestrzennej.Wydział Filologiczny, Uniwersytet w Białymstoku95-10599510
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