228 research outputs found
Pax8 modulates the expression of Wnt4 that is necessary for the maintenance of the epithelial phenotype of thyroid cells
No full textBackground: The transcription factor Pax8 is expressed during thyroid development and is involved in the morphogenesis of the thyroid gland and maintenance of the differentiated phenotype. In particular, Pax8 has been shown to regulate genes that are considered markers of thyroid differentiation. Recently, the analysis of the gene expression profile of FRTL-5 differentiated thyroid cells after the silencing of Pax8 identified Wnt4 as a novel target. Like the other members of the Wnt family, Wnt4 has been implicated in several developmental processes including regulation of cell fate and patterning during embryogenesis. To date, the only evidence on Wnt4 in thyroid concerns its down-regulation necessary for the progression of thyroid epithelial tumors. Results: Here we demonstrate that Pax8 is involved in the transcriptional modulation of Wnt4 gene expression directly binding to its 5'-flanking region, and that Wnt4 expression in FRTL-5 cells is TSH-dependent. Interestingly, we also show that in thyroid cells a reduced expression of Wnt4 correlates with the alteration of the epithelial phenotype and that the overexpression of Wnt4 in thyroid cancer cells is able to inhibit cellular migration. Conclusions: We have identified and characterized a functional Pax8 binding site in the 5'-flanking region of the Wnt4 gene and we show that Pax8 modulates the expression of Wnt4 in thyroid cells. Taken together, our results suggest that in thyroid cells Wnt4 expression correlates with the integrity of the epithelial phenotype and is reduced when this integrity is perturbed. In the end, we would like to suggest that the overexpression of Wnt4 in thyroid cancer cells is able to revert the mesenchymal phenotype
Grazia Deledda’s autobiographical masks
No full textAll’interno della produzione di Grazia Deledda, Cosima è il personaggio più autobiografico, come si può notare dagli esempi riportati. È anche possibile scorgere assonanze tra l’autrice ed altre tre figure femminili protagoniste delle sue opere: Regina, protagonista di Nostalgie, Nina di Il Paese del Vento e Maria Concezione di La chiesa della solitudine.Among Grazia Deledda’s characters, Cosima is the most autobiographical one, as we can notice from the examples. Concordance can be noticed between the author and the female characters of her works: Regina is the main character of Nostalgie, Nina in Il Paese del Vento and Maria Concezione in La chiesa della solitudine
Maria Grazia Turri, Gli oggetti che popolano il mondo. Ontologia delle relazioni, Roma, Carocci, 2012, pp. 239
No full textThe text offers a Critical Review of "Gli oggetti che popolano il mondo. Ontologia delle relazioni" by Maria Grazia Turri. The author critically reflects on the book by considering its methodologies, its arguments, and its relation with other books of the same type and on the same subject.Il testo propone una Lettura Critica del libro "Gli oggetti che popolano il mondo. Ontologia delle relazioni" di Maria Grazia Turri. L'autrice riflette criticamente sul libro considerandone le metodologie, gli argomenti e il nesso con altri libri dello stesso tipo e sullo stesso argomento
A role for PAX8 in the tumorigenic phenotype of ovarian cancer cells
No full textBackground: PAX8 is a member of the paired box (Pax) multigene family of transcription factors, which are involved in the developmental and tissue-specific control of the expression of several genes in both vertebrates and invertebrates. Previously, several studies reported that PAX8 is expressed at high levels in specific types of tumors. In particular, PAX8 has been recently reported to be conspicuously expressed in human ovarian cancer, but the functional role of PAX8 in the carcinogenesis of this type of tumor has not been addressed. In this study, we investigated the contribution of PAX8 in ovarian cancer progression. Methods: Stable PAX8 depleted ovarian cancer cells were generated using short hairpin RNA (shRNA) constructs. PAX8 mRNA and protein were detected by RT-PCR, immunoblot and immunofluorescence. Cell proliferation, motility and invasion potential of PAX8 silenced cells were analyzed by means of growth curves, wound healing and Matrigel assays. In addition, PAX8 knockdown and control cells were injected into nude mice for xenograft tumorigenicity assays. Finally, qPCR was used to detect the expression levels of EMT markers in PAX8-overexpressing and control cells. Results: Here, we show that PAX8 plays a critical role in the migration, invasion and tumorigenic ability of ovarian cancer cells. Our results show that RNA interference-mediated knockdown of PAX8 expression in SKOV-3 ovarian cancer cells produces a significant reduction of cell proliferation, migration ability and invasion activity compared with control parental SKOV-3 cells. Moreover, PAX8 silencing strongly suppresses anchorage-independent growth in vitro. Notably, tumorigenesis in vivo in a nude mouse xenograft model is also significantly inhibited. Conclusions: Overall, our results indicate that PAX8 plays an important role in the tumorigenic phenotype of ovarian cancer cells and identifies PAX8 as a potential new target for the treatment of ovarian cancer
LE MASCHERE AUTOBIOGRAFICHE DI GRAZIA DELEDDA
No full textWithin the production of Grazia Deledda, Cosima is the most autobiographical character, as can be seen from the examples shown. It is also possible to see similarities between the author and three other female figures protagonists of her works: Regina, protagonist of Nostalgie, Nina of The Country of the Wind and Maria Concezione of The church of solitude.All’interno della produzione di Grazia Deledda, Cosima è il personaggio più autobiografico, come si può notare dagli esempi riportati. È anche possibile scorgere assonanze tra l’autrice ed altre tre figure femminili protagoniste delle sue opere: Regina, protagonista di Nostalgie, Nina di Il Paese del Vento e Maria Concezione di La chiesa della solitudine.
Abstract
Within the production of Grazia Deledda, Cosima is the most autobiographical character, as can be seen from the examples shown. It is also possible to see similarities between the author and three other female figures protagonists of her works: Regina, protagonist of Nostalgie, Nina of The Country of the Wind and Maria Concezione of The church of solitude
Appunti per una storia dello spazio: piazza Tahrir e reti virtuali nell'Egitto della rivoluzione
No full textIl lavoro si presenta come un’analisi della rivoluzione di piazza Tahrir, alla riscoperta della riappropriazione dello spazio della piazza da parte della popolazione, attraverso l’utilizzo combinato dei social network e dei media tradizionali. La prima parte a cura di Mattia Bonfanti analizza il contesto dei 18 giorni di rivoluzione al Cairo. Il mancato ascolto delle istanze democratiche e un contesto politico affetto da cronica corruzione e miopia della classe dirigente egiziana, ha portato allo scoppio della protesta di piazza Tahrir il 25 gennaio 2011, i cui esiti restano a tutt’oggi controversi. La seconda parte a cura di Maria Grazia Montella si delinea come una riflessione sull’exploit dei social media come “ritorno alla fisicità” e riscoperta delle manifestazioni spaziali e fisiche del dissenso. La connessione tra lo spazio virtuale della rete e lo spazio fisico della piazza è storica, politica e culturale e si innesta nelle riflessioni prese in prestito da Lefebvre e Foucault.The work presents an analysis of the Egyptian uprising and of its most known rich place Tahrir Square. The process of re-appropriation of the space of the square through the combined use of social networks and traditional media is traced through the division in two parts. The first one outlines the political context and the eighteen days of the uprising in Cairo. The author identifies the principal causes of the revolution burst in the failure in listening the people’s request for democracy and in the chronic corruption of the government. The second part argues that the massive usage of the social networks has to be seen as a back to the physical, a revival of spatial and physic demonstration of the dissent. The connection between the virtual space of the internet and the real space of the square is historical, political, and cultural and it has to be sought in Foucault and Lefebvre reflections
The Enantioselective Mukaiyama-Michael Reaction between Crotonyl-1,3-oxazolidin-3-one and 2-Trimethylsilyloxyfuran. The Importance of both Cations and Substituents on the C2-Symmetric Bisoxazoline Ligands
No full textMigration as a risk for security. Risk frames in the Italian news on the Libya war and its aftermath
No full textDi fronte a crisi e conflitti internazionali che ridefiniscono i movimenti migratori verso l’Europa, esaminare la rappresentazione delle migrazioni nei mezzi di comunicazione può essere di grande utilità per comprendere come i rischi per la sicurezza sono percepiti, valutati e messi in connessione nel discorso pubblico. L’autrice offre un contributo in questa direzione attraverso l’analisi del framing delle notizie della stampa italiana durante e dopo l’intervento internazionale in Libia (2011-2012). Pur se molteplici aspetti della relazione migrazioni-sicurezza coesistono nei media, l’analisi automatica del contenuto degli articoli di cinque quotidiani nazionali consente di evidenziare la prevalenza di un frame delle migrazioni come rischio per i paesi di destinazione. L’enfasi su questioni di sicurezza come il terrorismo, la criminalità e l’ordine pubblico, inoltre, contribuiscono a caratterizzare le migrazioni come una vera e propria minaccia, riaffermando la dominanza di un discorso noi-centrico e securitario sul fenomeno migratorio.As crises and conflicts in the Middle East and across Africa reshape migration flows to Europe, looking how migration are represented in the media is of crucial importance to understand how multiple risks to security are understood, evaluated and connected to each other in the public discourse. The author contributes to this effort by analysing the framing of migration risks in the Italian press in a two-year period during and following the international military intervention in Libya in 2011. The examination of frames concerning various security risks through automated content analysis, allows to assess the relative relevance of a migration-as-a-risk frame in the press coverage. Albeit frames dealing with different aspects of the security-migration binomial coexist, the author shows how those concerned with direct threats to destination societies are the most frequent, thus revealing the dominance of a we-centric discourse over migration in the media
Il mio ricordo del Professor Mario Galizia
No full textL'autore ricorda gli insegnamenti del maestro nelgli anni universitari con riferimento al ruolo degli organi di controllo della costituzionalità in particolare nella Francia della V RepubblicaThe author recalls the teachings of Professor Mario Galizia in university years with reference to the role of the supervisory bodies of the constitutionality in particular in France of the Fifth Republi
Qualche nota sui prestiti di origine longobarda nel Glossario Bergamasco Medioevale di Antonio Tiraboschi
No full textAntonio Tiraboschi (1838–1883), one of the most prolific scholars living in Bergamo in the second half of the Nineteen century and the author of the Vocabolario dei Dialetti Bergamaschi Antichi e Moderni (1873) [Dictionary of the ancient and modern Bergamo dialects], is a well-known Romance dialectologist. His research interests, however, included other disciplinary areas, such as palaeography and archival sciences, which
enabled him to compile a Medieval Bergamasco Glossary [Glossario Bergamasco Medioevale], whose manuscript, yet unpublished, is deposited in the local Biblioteca Civica Angelo Mai. In this contribution to the memory of Maria Vittoria Molinari, we provide a description of the semantic domains to which the lexemes of Langobard origins mentioned in the Glossary belong. Most of these lexemes are attested in other Italian
regions, and hence, well-documented in the literature. Accordingly, we will focus on Tiraboschi’s dialectological and linguistic comments, as well as on the linguistic traits displayed by the extracts quoted in order to exemplify each lexical entr
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