238 research outputs found
Regulation of growth, patterning and cell fate-specification during imaginal disc regeneration in drosophila
No full textThe process of regeneration is immensely complicated and requires the exquisitely precise orchestration of growth, patterning and cell-fate specification. Drosophila melanogaster larval tissues have recently emerged as a useful model organism to study this process. These larval tissues are simple but experience complex gene expression to finally form the adult tissue. As such, Drosophila is an ideal model organism to study the complicated process of regeneration. In this study we have used the experimental tractability of Drosophila to investigate how growth, patterning, cell-fate specification and fate plasticity are regulated in regenerating imaginal discs.
Regenerative growth must be carefully controlled and constrained to prevent overgrowth and to allow correct organization of the regenerating tissue. However, the factors that restrict regenerative growth have not been identified. In this study, we have identified one mechanism that constrains regenerative growth, impairment of which leads to erroneous patterning of the final appendage. Regenerating discs with reduced levels of the RNA-regulator Brain tumor (Brat) exhibit enhanced regeneration but produce adult wings with disrupted margins. The loss of cell-fate specification is due to the aberrantly high expression of the pro-growth factor Myc and the self-renewal factor Chinmo. Thus, Brat ensures that the regenerating tissue forms the proper final structure by constraining expression of pro-regeneration genes.
Dissociation of imaginal disc cells has previously been carried out to enable flow cytometry and cell sorting to analyze cell cycle progression, cell size, gene expression, and other aspects of imaginal tissues. However, the lengthy dissociation protocols employed may alter gene expression, cell behavior and overall viability. In this study, we developed a new rapid and gentle method of dissociating the cells of wing imaginal discs that significantly enhanced cell viability and reduced the likelihood of gene expression changes. This method was successfully used to create a transcriptional profile of the regenerating tissue leading to the identification of many novel regulators of regeneration. We have also extended our investigation of regeneration to the antennal imaginal disc.
Drosophila imaginal discs have also been used to study cell-fate specification and plasticity, including homeotic changes and regeneration-induced transdetermination. In this study, we identified a change from antennal fate to eye fate induced by a Distal-less-GAL4 (DllGAL4) P-element insertion that is a mutant allele of Dll and expresses GAL4 in the antennal imaginal disc. While this fate change was not induced by tissue damage, it appeared to be a hybrid of transdetermination and homeosis. This plasticity appears to be unique to the DllGAL4 line, possibly due to cellular stress induced by the high GAL4 expression combined with the severity of the Dll mutation. Thus, we propose that even in the absence of tissue damage, other forms of cellular stress caused by high GAL4 expression can induce determined cell fates to change, and selector gene mutations can sensitize the tissue to these transformations.Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2021-12-01The student, Syeda Nayab Abidi, accepted the attached license on 2019-11-07 at 12:27.The student, Syeda Nayab Abidi, submitted this Dissertation for approval on 2019-11-07 at 12:54.This Dissertation was approved for publication on 2019-11-11 at 14:20.DSpace SAF Submission Ingestion Package generated from Vireo submission #14532 on 2020-02-28 at 17:21:56Made available in DSpace on 2020-03-02T22:12:16Z (GMT). No. of bitstreams: 2
ABIDI-DISSERTATION-2019.pdf: 15945876 bytes, checksum: 5826949b9a29ed27ed4cf0b9eb45ff84 (MD5)
LICENSE.txt: 4214 bytes, checksum: f63d6e672684a60081d0e86da9589e7b (MD5)
Previous issue date: 2019-11-11Embargo set by: Seth Robbins for item 113879
Lift date: 2022-03-02T22:12:26Z
Reason: Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD systemEmbargo set by: Seth Robbins for item 113879
Lift date: 2022-03-02T22:15:21Z
Reason: Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD systemEmbargo set by: Seth Robbins for item 113879
Lift date: 2022-03-02T22:18:25Z
Reason: Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD systemU of I Only Restriction Lifted for Item 113879 on 2022-03-03T10:15:13Z
Author Correction: a Detailed Map of Higgs Boson Interactions by the Atlas Experiment Ten Years After the Discovery (nature, (2022), 607, 7917, (52-59), 10.1038/S41586-022-04893-w)
No full textCorrection to: Nature Published online 4 July 2022 In the version of this article initially published, the ATLAS Collaboration author names, affiliations and acknowledgements were omitted and have now been included in the HTML and PDF versions of the article. © 2023, The Author(s)
Efficient and Effective Cohesive Subgraph Search in Bipartite Graphs
No full textBipartite graphs are an interesting structure that can model the relationships between two different types of entities in real-world scenarios such as customer-product and author-paper networks. Finding bipartite cohesive subgraphs is a fundamental problem aiming to find strongly connected subgraphs to better understand such bipartite graphs. The powerful traditional models for searching strongly connected patterns cannot fully explore the deeper insights of bipartite graphs, as they are defined on general graphs. Therefore, in this thesis, we first study the fundamental problem of enumerating all bipartite cohesive subgraphs and then present two new bipartite cohesive subgraph models for distinct real-world scenarios
How Pakistan\u27s media spreads the message about reproductive and sexual health
No full textAbstract, issue, and pagination are not provided by the author/publishe
Al-Ghazali's Thought About Sufistic Education Character in The Book Minhajul Abidin
No full textEducation character has become an important issue in the world of education lately, with the increasing and varied phenomena of moral decadence occurring in society and in government circles, especially among students. The government pays less attention to fostering education character. The government is more focused on continuous curriculum reform. This condition will get worse if the government, parents and schools or madrasah receive less serious attention. Sufistic education character is an important approach to be applied to overcome these moral decadency phenomena. Therefore, this study aims to provide alternative solutions in realizing children's education character both in the family environment, school environment and social environment. In addition, in this study the author offers several concepts of education character with the Sufistic character of al-Ghazali as in the book of Mohajul Abidin: first, preserving the eyes. Second, maintain the ears. Third, take care of the mouth. Fourth, maintain the stomach.Keywords: Biography of Al-Ghazali, Sufistic Education character in the book Minhajul Abidi
El machismo en los personajes femeninos principales de Tristana de Benito Pérez Galdós y La última niebla de María Luisa Bombal
No full textThe purpose of this work is to explore the roots of sexism and the situation of women in Spain and Chile during the late 19th and early 20th centuries, as reflected in literature. To achieve this objective, this paper conducted a comparative analysis of two novels of two renowned authors in Hispanic literature: Tristana by the Spanish author Benito Pérez Galdós and La última niebla by the Chilean María Luisa Bombal. This idea stems from the theory that suggests Bombal’s work incorporates elements from Galdós. By comparing the main female characters of both novels, we aim to identify similarities in the portrayal of sexism by these authors within their respective societiesEl propósito de este trabajo es realizar un acercamiento al estado de la cuestión que busca indagar en las raíces del machismo y la situación de la mujer en España y Chile a fines del siglo xix y principios del siglo xx, a través de su reflejo en la literatura.
Para ello, hemos decidido llevar a cabo un análisis comparativo de dos novelas de dos destacados autores de la literatura hispana: Tristana, del escritor español Benito Pérez Galdós y La última niebla, de la autora chilena María Luisa Bombal.
Esta idea surge de la teoría que plantea que la obra de Galdós presenta características retomadas por Bombal. De esta manera, a través de la comparación entre las protagonistas principales de ambas novelas, buscaremos identificar las similitudes en el tratamiento del machismo por parte de ambos autores en sus respectivas sociedades
El machismo en los personajes femeninos principales de Tristana de Benito Pérez Galdós y La última niebla de María Luisa Bombal
No full textThe purpose of this work is to explore the roots of sexism and the situation of women in Spain and Chile during the late 19th and early 20th centuries, as reflected in literature. To achieve this objective, this paper conducted a comparative analysis of two novels of two renowned authors in Hispanic literature: Tristana by the Spanish author Benito Pérez Galdós and La última niebla by the Chilean María Luisa Bombal. This idea stems from the theory that suggests Bombal’s work incorporates elements from Galdós. By comparing the main female characters of both novels, we aim to identify similarities in the portrayal of sexism by these authors within their respective societies.El propósito de este trabajo es realizar un acercamiento al estado de la cuestión que busca indagar en las raíces del machismo y la situación de la mujer en España y Chile a fines del siglo xix y principios del siglo xx, a través de su reflejo en la literatura.Para ello, hemos decidido llevar a cabo un análisis comparativo de dos novelas de dos destacados autores de la literatura hispana: Tristana, del escritor español Benito Pérez Galdós y La última niebla, de la autora chilena María Luisa Bombal.Esta idea surge de la teoría que plantea que la obra de Galdós presenta características retomadas por Bombal. De esta manera, a través de la comparación entre las protagonistas principales de ambas novelas, buscaremos identificar las similitudes en el tratamiento del machismo por parte de ambos autores en sus respectivas sociedades
CNN-resNet-50 Algorithm to Detect the Novel Coronavirus (COVID-19) Using CT Images
No full textThe detection of the Covid-19 pandemic has been crucial in ensuring health safety and preventing the spread of the virus. In this paper, we present a novel approach by combining the CNN and ResNet-50 algorithms into a unified model. Our experimental results showcase the remarkable efficiency of the proposed method, outperforming both the individual CNN and ResNet-50 approaches. The hybrid CNN-ResNet-50 algorithm demonstrates its ability to automatically and effectively assist in the early diagnosis of COVID-19 patients. By leveraging this combined model, we aim to contribute to the improved detection and management of the pandemic
Needs Assessment and System Design: Implementation of an Electronic Charting and Database System at a Canadian Poison Centre
No full textInternship report - Fall 2005 and Winter 2006The IWK Regional Poison Centre at the time of this paper kept only paper-based patient care records. The growing number of queries regarding statistical data on poisonings in Nova Scotia and Prince Edward Island prompted the plan to implement an electronic charting and database system. The internship undertaken by the author, was to perform initial tasks related to the implementation of an electronic charting and database system. The author conducted a needs assessment of the IWK Regional Poison Centre with regards to the new computerized system and presented recommendations to the Clinical Leader and Manager of the poison centre along with representatives of the IWK Health Centre Information Technology (IT), Health Records and Quality Resources Departments. Dr. Raza Abidi provided feedback related to the project progress throughout the internship. Through research conducted regarding the current use of electronic charting and database systems in poison centres throughout Canada and the United States the Visual Dotlab system was located and approved for implementation. In performing her duties at the IWK Regional Poison Centre the author discovered that web technology, which may have best suited the needs of specific healthcare organizations or departments was being under-used based on misconceptions held by both healthcare and IT professionals. This prompted further research into the use of web technology in the healthcare field and advocacy for increased awareness of the benefits of this technology for specified use.IWK Regional Poison Centr
- …
