1,720,981 research outputs found
A new automated method for isolation of genomic DNA from eukaryotic cells.
No full textIn this study we determine whether the BIOMEK-1000 Laboratory Workstation (Beckman Instruments, Fullerton, CA) can be used to isolate genomic DNA from eukaryotic cells. The results obtained demonstrate that DNA isolated by BIOMEK-1000 is suitable for amplification of genomic sequences by PCR. Our data prove that automated chromatographic DNA isolation employing the BIOMEK-1000 could be used in molecular diagnosis of human pathologies
Friend erythroleukemic cells treated with aromatic polyamidines exhibiting antiproteinase activity: inhibition of cell growth is not associated with block of induced erythroid differentiation
No full textIn this paper we report the effects of aromatic poly amidines (TAPP-H, TAPB-H and DAPP-H) and their bromo-derivatives (TAPP-Br, TAPB-Br and DAPP-Br) on cell growth and erythroid differentiation of murine Friend erythroleukemic cells. These compounds are strong inhibitors of serine proteinases. The results obtained give evidence (a) for inhibition of cell proliferation by all the aromatic poly-amidines studied; (b) for stronger antiproliferative activity of the Br-derivatives; (c) for a lack of inhibition of globin mRNA accumulation and hemoglobin synthesis is uninduced cells as well as in Friend cells induced to erythroid differentiation by hexamethylenebisacetamide. These results suggest that this class of antiproliferative compounds exhibits differential effects on cell-cycle and differentiation specific genes. Thus, these aromatic poly-amidines could be used in experimental therapy without interfering with differentiative processes also when combined with differentiating agents
Use of the A.L.F. DNA sequencing system for DNase I footprinting studies
No full textMolecular...Molecular biology techniques have been largely employed to study protein-DNA interactions in projects related to experimental oncology, differentiation and development, hereditary diseases, design and synthesis of anticancer and antitumor agents. DNase I footprinting assay has been extensively used to study interactions between P-32-labelled target DNA and (a) crude nuclear extracts, (b) recombinant DNA-binding proteins, (c) peptides, (d) DNA-binding drugs and (e) triple helix-forming oligonucleotides. In this paper we review the use of automated DNA sequencing systems to develop a non-radioactive protocol to study protein-DNA interactions by DNase I footpriting. In addition, automated DNA sequencing systems were employed to compared the in vitro effects of two DNA-binding drugs, distamycin and chromomycin. The results obtained clearly indicate that the A.L.F. DNA Sequencing System (Pharmacia LKB, Uppsala, Sweden) could be used to efficiently perform non-radioactive DNase I footpri..
Use of an automated laboratory workstation for isolation of genomic DNA suitable for PCR and allele-specific hybridization
No full textIn this paper we describe the isolation of genomic DNA by using anion exchange chromatography performed on a Biomek 1000 Automated Laboratory Workstation. This procedure allows the automated isolation of DNA suitable for most molecular analyses employed in diagnosis of genetic pathologies and infectious diseases. The genomic DNA isolated by using the Biomek 1000 was indeed found to be suitable for polymerase chain reaction and allele-specific hybridization
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Distamycin-treated MCF7 cells: inhibition of PCR-mediated amplification of a TA-rich region of human estrogen receptor gene
No full textRecent..
Peptide Nucleic Acids and Biosensor Technology for Real-Time Detection of the Cystic Fibrosis W1282X Mutation by Surface Plasmon Resonance
No full textVariations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Potential role of circulating microRNAs as early markers of preeclampsia
No full textObjective: To identify microRNAs (miRNAs) differentially expressed at early stages of gestation (12-14 weeks) in the serum of pregnant women, who later developed severe preeclampsia (sPE) in the third trimester of pregnancy (n = 24) compared to women with normal pregnancy (n = 24). Materials and methods: Sera from 12-14-week-gestation whole blood were subjected to microarray analysis with TaqMan Low Density Array chips (human microRNA panel V3.0), and to quantitative real-time polymerase chain reaction. Results: By using the TaqMan Low Density Array chip technology, 19 mature miRNAs appeared differentially expressed in the group of women who later developed sPE as compared to normal women. The expression of four miRNAs (miR-1233, miR-520, miR-210, miR-144) was validated by quantitative real-time polymerase chain reaction analysis. MiR-1233 was the most overexpressed in the serum of women who later developed sPE. Conclusion: Circulating miRNAs deserve further investigation in order to explore their potential role in the pathogenesis of preeclampsia. In particular, miR-1233 might represent a potential marker of early sPE.Objective: To identify microRNAs (miRNAs) differentially expressed at early stages of gestation (12-14 weeks) in the serum of pregnant women, who later developed severe preeclampsia (sPE) in the third trimester of pregnancy (n=24) compared to women with normal pregnancy (n=24). Materials and Methods: Sera from 12-14-week-gestation whole blood were subjected to microarray analysis with TaqMan Low Density Array chips (human microRNA panel V3.0), and to quantitative real-time polymerase chain reaction. Results: By using the TaqMan Low Density Array chip technology, 19 mature miRNAs appeared differentially expressed in the group of women who later developed sPE as compared to normal women. The expression of four miRNAs (miR-1233, miR-520, miR-210, miR-144) was validated by quantitative real-time polymerase chain reaction analysis. MiR-1233 was the most overexpressed in the serum of women who later developed sPE. Conclusion: Circulating miRNAs deserve further investigation in order to explo..
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