102,359 research outputs found

    Capacidade de promover biomineralização de diferentes bioagregados: análise in vivo

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    Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências da Saúde. Programa de Pós-Graduação em OdontologiaO objetivo deste estudo foi analisar a interação dos cimentos MTA Fillapex, iRoot SP, DiaRoot Bioaggregate (BA) e MTA Branco com a dentina in vivo. Para isso, a partir de dentes humanos, foram confeccionados 160 tubos de dentina que posterior e aleatoriamente foram divididos em 4 grupos experimentais e 1 grupo-controle. Após o preenchimento com os materiais, os tubos foram implantados no tecido conjuntivo subcutâneo de 32 ratos, em 4 sítios equidistantes da área dorsal. Tubos vazios foram usados como controle e implantados em um quinto sítio. Após 7, 15, 30 e 90 dias os animais foram sacrificados e os tubos de dentina (n = 8/grupo) foram retirados e preparados para análise em microscópio eletrônico de varredura (MEV). Em 7 e 30 dias, a deposição mineral na interface material-dentina foi detectada em todos os tubos preenchidos com MTA Fillapex; e em 15 e 90 dias foi detectada em 7 das 8 amostras. Independentemente do período, a deposição mineral não foi visualizada em nenhuma amostra dos grupos iRoot SP, BA e MTA Branco e nem nas do grupo-controle. Foi concluído que o MTA Fillapex foi o único cimento que interagiu com a dentina e promoveu a biomineralização in vivo, e que este processo não foi influenciado pelo tempo.The aim of this study was to analyze the interaction of the cements MTA Fillapex, iRoot SP, DiaRoot Bioaggregate (BA) and MTA Branco with dentin in vivo. One hundred and sixty human dentin tubes were randomly divided in 4 experimental and 1 control group. After filled with the materials, the tubes were implanted subcutaneously in 32 rats at four sites equidistant from the dorsal area. Empty tubes were used as control and implanted in a fifth site. After 7, 15, 30 and 90 days the animals were euthanized and the dentin tubes (n = 8/group) retrieved for scanning electron microscope (SEM) analysis. In 7 and 30 days, the mineral deposition in the material-dentin interface was detected in all tubes filled with MTA Fillapex; and in 15 and 90 days it was detected in 7 of the 8 samples. Despite of the period, the mineral deposition was not detected in any of the tubes filled with iRoot SP, BA and MTA Branco neither in those of control group. It was concluded that MTA Fillapex was the only cement which interacted with dentin and promoted the in vivo biomineralization, and that this process was not affected by the time

    Stilobezzia (Eukraiohelea) proxima Cazorla & Felippe-Bauer

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    Stilobezzia (Eukraiohelea) proxima Cazorla & Felippe-Bauer (Figs. 44–45) Stilobezzia (Eukraiohelea) proxima Cazorla & Felippe-Bauer in: Cazorla et al. 2017: 562 (male, female, Brazil); Borkent & Dominiak 2020: 177 (in Online World Catalog); Santarém & Felippe-Bauer 2022: 19 (Brazilian Catalog; distribution). Type. Holotype male, labeled: “ Stilobezzia (Eukraiohelea) proxima Cazorla & Felippe-Bauer, BRAZIL, Rio de Janeiro, Casimiro de Abreu, Union Biological Reserve (22°25′35″ S; 42°02′04″ W), 07.xi–13.xii.2013, “Biota Diptera Fluminense ” team col., Malaise trap ”, (CCER). Allotype female; paratypes: 1 male, same data as holotype (CCER), 1 male, 1 female same data except 25.viii–26.xi.2013 ”, (CCER). Material examined. BRAZIL, Amazonas, Manaus, Campina Biological Reserve, 02º36′19″ S – 60º02′11″ W, Malaise trap, 04–18.ix.2018, I.M. Da Silva and R.L. Ferreira-Keppler, 1 male; same data except 04–18.x.2018, 4 females. Comments. This Neotropical species was described by Cazorla & Felippe-Bauer (2017), and its type locality is in Rio de Janeiro, Brazil. We provide the first record of this species from the state of Amazonas, that was collected in the Campina Biological Reserve, in the rural region of Manaus.Published as part of Da Silva, Irene M., Ferreira-Keppler, Ruth L. & Cazorla, Carla G., 2023, Stilobezzia Kieffer (Diptera: Ceratopogonidae) from the Brazilian Amazon: three new species, redescription of S. (Stilobezzia) maculata Lane and new records, pp. 485-499 in Zootaxa 5249 (4) on page 494, DOI: 10.11646/zootaxa.5249.4.6, http://zenodo.org/record/769462

    Eficácia de diferentes técnicas de irrigação sobre a remoção da pasta de hidróxido de cálcio do canal radicular

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    TCC (graduação) - Universidade Federal de Santa Catarina. Centro de Ciências da Saúde. Odontologia.Há relatos na literatura de que resíduos da pasta de Hidróxido de Cálcio (HC) sobre as paredes do canal radicular diminuem a força de união dos materiais obturadores à dentina e impedem a sua penetração nos túbulos dentinários, comprometendo a qualidade do selamento do canal. Por isso, torna-se necessária a remoção completa da pasta antes da obturação endodôntica. O objetivo deste estudo foi analisar, por meio de imagens digitais obtidas em estereomicroscópio, a eficácia de diferentes protocolos de irrigação na remoção da pasta de HC do interior do canal radicular. Foram utilizados 44 raízes de dentes humanos, completamente formadas, e com comprimento padronizado em 17 mm. Após o acesso, os canais foram instrumentados com limas Flexofile e K até o #45 (Instrumento memória - IM), e irrigados com 2 mL de hipoclorito de sódio 1% (NaOCl) entre o uso de cada lima. O preparo dos terços cervical e médio foi finalizado com brocas Gates-Glidden. Por fim os canais foram irrigados com 3 mL de solução de ácido etilenodiaminotetracético 17% (EDTA) por 3 minutos, seguidos de 3 mL de NaOCl 1%, também por 3 minutos. Após a secagem, 42 canais foram preenchidos com pasta de HC e, efetuado o selamento coronal com Coltosol, os dentes foram mantidos em 100% de umidade a 37ºC. Passados 7 dias, 40 canais foram divididos em 4 grupos experimentais (n = 10), de acordo com o protocolo de irrigação utilizado para a remoção da pasta: G1- 2,5 mL de NaOCl 1%, agitação com o IM até o comprimento de trabalho de modelagem (CTM) por 30 segundos, irrigação com 2,5 mL de NaOCl 1%, nova agitação com o IM por 30 segundos e irrigação final com 5 mL de NaOCl 1%; G2 - idem ao G1, porém a irrigação final foi realizada com 5 mL de EDTA 17%; G3 - 2,5 mL de NaOCl 1%, agitação ultrassônica até 2 mm aquém do CTM por 30 segundos, irrigação com 2,5 mL de NaOCl 1%, nova agitação ultrassônica por 30 segundos, e irrigação final com 5 mL de NaOCl 1%; G4 - idem ao G3, porém a irrigação final foi realizada com 5 mL de EDTA 17%. Dois dentes serviram como controle positivo, no qual a pasta não foi removida e em outros 2 os canais foram mantidos vazios, servindo como controle negativo. Efetuada a secagem, a entrada de cada canal foi protegida com bolinha de algodão e selada com Coltosol. As raízes foram seccionadas no sentido vestíbulo-lingual em 2 metades. A superfície dentinária de uma das metades foi examinada em estereomicroscópio e fotos digitais foram realizadas para o cálculo do percentual de hidróxido de cálcio remanescente sobre a parede de dentina de cada terço do canal. Os dados foram analisados estatisticamente pelos testes ANOVA1 e de Tukey HSD, num nível de significância de 5%. Remanescentes da pasta de HC foram encontrados em todas as amostras dos grupos experimentais, sem diferença significante entre os grupos (P = 0,690). Em todos os grupos experimentais, o terço apical exibiu maior percentual do que o terço médio e o cervical, os quais exibiram percentuais similares. Foi concluído que nenhum dos protocolos empregados foi capaz de remover completamente o HC do interior dos canais radiculares e que o terço apical apresentou maior percentual de remanescentes de pasta de HC./There are reports in the literature that residues of the calcium hydroxide paste (HC) on root canal walls decrease the bond strength of root canal fillings to the dentin, and prevent their penetration in the dentinal tubules, compromising the quality of root canal sealing. Therefore, the complete removal of this paste before endodontic obturation becomes necessary. The aim of this study was to analyze, through digital images obtained in stereomicroscope, the effectiveness of different irrigation protocols on removal of the HC of the interior of the root canal. A total of 44 roots of human teeth was used. After the coronal access, the canals were instrumented with Flexofile files and K until to 45 size (MAF), and irrigated with 2 mL of 1% sodium hypochlorite (NaOCl) between the use of each file. The preparation of thirds cervical and medium was finalized with Gates-Glidden drills. Finally the canals were irrigated with 3 mL of 17% ethylenediaminetetraacetic acid (EDTA) for 3 min, followed by 3 mL of NaOCl, also for 3 min. After drying, 42 root canals were filled with HC, the coronal access was sealed with Coltosol, and the roots were kept at 37ºC with 100% humidity. After 7 days, 40 root canals were divided into 4 experimental groups (n = 10), according to the irrigation protocol used for removing of the paste: G1- 2.5 mL of 1% NaOCl, use of MAF until to working length (WL) for 30 seconds, irrigation with 2.5 mL of NaOCl, use of MAF for 30 seconds and final irrigation with 5 mL of NaOCl; G2 - Equal to G1, however the final irrigation was performed with 5 mL of 17% EDTA; G3 - 2.5 mL of 1% NaOCl, ultrasonic agitation for 30 seconds, irrigation with 2.5 mL of NaOCl, a new ultrasonic agitation for 30 seconds, and final irrigation with 5 mL of NaOCl; G4 - Equal to G3, but final irrigation was performed with 5 mL of 17% EDTA. Two roots served as positive control, in which the paste was not removed and other 2 were kept empty, serving as negative control. After drying, the entrance of each root canal was protected with cotton balls and sealed with Coltosol. Roots were sectioned in the bucco-lingual direction in 2 halves. The dentin surface of one of the halves was examined in stereomicroscope and digital photos were taken for the subsequent calculation of the percentage of remaining calcium hydroxide on the wall of dentine in each third of the canal. The data were statistically analyzed by ANOVA1 and Tukey HSD tests at a level of significance of 5%. Remnants of the HC were found in all samples of experimental groups, with no significant difference between the groups (P 0.690). In all experimental groups, the apical third exhibited a higher percentage of remnants than the middle and the cervical thirds, which exhibited similar percentages. It was concluded that none of the protocols employed was able to completely remove the HC of the root canals, and that the apical third showed the highest percentage of remnants of HC paste

    Stilobezzia (Eukraiohelea) quasielegantula Cazorla & Felippe-Bauer 2017

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    <i>Stilobezzia</i> (<i>Eukraiohelea</i>) <i>quasielegantula</i> Cazorla & Felippe-Bauer <p>(Fig. 46)</p> <p> <i>Stilobezzia</i> (<i>Eukraiohelea</i>) <i>quasielegantula</i> Cazorla & Felippe-Bauer 2017: in Cazorla <i>et al.</i> 2017: 566 (male, female, Brazil). Borkent & Dominiak 2020: 177 (in Online World Catalog); Santarém & Felippe-Bauer 2022: 19 (Brazilian biting midges Catalog; distribution).</p> <p> <b>Type</b>. Holotype male, labeled: “ <i>Stilobezzia</i> (<i>Eukraiohelea</i>) <i>quasielegantula</i> Cazorla & Felippe-Bauer, BRAZIL, Rio de Janeiro, Casimiro de Abreu, Union Biological Reserve (22°25′35″ S – 42°02′04″ W), 07.xi–13.xii.2013, “Biota Diptera Fluminense ” team col., Malaise trap ”, (CCER). Allotype female, 30 paratypes: 9 males, 8 females, same data as holotype (7 males, 6 females (CCER); 2 males, 2 females, (MLPA); 1 male, 1 female same data except 25.viii–26.xi.2013 (CCER).</p> <p> <b>Material examined</b>. BRAZIL, Amazonas, Manaus, Campina Biological Reserve; 02º36′19.01″ S <b>–</b> 60º02′11.74″ W, suspensa trap, 23.viii–04.ix.2018, I.M. Da Silva and R. L. Ferreira-Keppler, 1 male, 2 females; Campinarana, Malaise trap, 04–23.x.2018, 1 male.</p> <p> <b>Comments</b>. This Neotropical species was described by Cazorla & Felippe-Bauer (2017), with prior knowledge for the state of Rio de Janeiro, in Brazil. We provide the first record of this species from the state of Amazonas, that was collected in the Campina Biological Reserve located, in the rural region of Manaus.</p>Published as part of <i>Da Silva, Irene M., Ferreira-Keppler, Ruth L. & Cazorla, Carla G., 2023, Stilobezzia Kieffer (Diptera: Ceratopogonidae) from the Brazilian Amazon: three new species, redescription of S. (Stilobezzia) maculata Lane and new records, pp. 485-499 in Zootaxa 5249 (4)</i> on page 495, DOI: 10.11646/zootaxa.5249.4.6, <a href="http://zenodo.org/record/7694629">http://zenodo.org/record/7694629</a&gt

    Monohelea barbara Felippe-Bauer & Cardoso & Trindade 2017, sp. nov.

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    Monohelea barbara Felippe-Bauer & Trindade sp. nov. (Figs.1; 2 A–G; 9A–B) Diagnosis. The only Neotropical species of Monohelea in which the males have brown legs with yellowish bands, two process in distal portion of the paramere, one beak-shaped and the other hook-shaped. Female unknown. Male. Head: eyes widely separated (Fig. 2 C). Antenna brown; flagellomeres 1–13 with lengths 108- 35- 35- 35- 30- 30- 28- 28- 25- 38- 93- 95- 110 (Fig. 2 B). Antenal ratio 0.93. Palpus (Fig. 2 D) pale brown; 3rd segment nearly oval with small, shallow, sensory organ on mid length; palpal ratio 1.25. Thorax. Without definite pattern on slide mounted specimen. Legs (Fig. 2 E) pale brown; fore-, mid coxae and trochanters brown; hind coxa and trochanter missing; femora with mesal and apical yellowish bands; hind femur with a brown ventral spot in the apical yellowish band; tibiae with basal and mesal yellowish bands; tibiofemoral joints yellowish; hind tibial comb with 7 bristles. Tarsi: pale; fore-, hind tarsomere 1 with one basal, one apical spine; midtarsomere 1 with 2 basal, 2 apical spines; apical spines of tarsomeres 2-4 of fore-, mid legs: 1-1-1, 2-2-2, basal spines absent; tarsomeres 2-4 of hind leg, missing; fore-, mid tarsal ratios 2.16, 2.43; claws small, paired, equal-sized, 0.35-0.39X as long as 5th tarsomeres. Wing (Fig. 2 A): macrotrichia restricted to vein costa; microtrichia absent; 2nd radial cell nearly 1.8X longer than 1st; wing length 0.83 mm, width 0.33 mm; costal ratio 0.75. Halter missing. Abdomen. Brown (Fig. 2 F). Genitalia (Figs. 2 G; 9 A–B): sternite IX spiculate except on basal portion, posterior margin not defined; tergite IX tapering with a pair of apicolateral processes, each with 4 setae. Gonocoxite moderately stout, nearly 2X longer than basal width; gonostylus straight 0.60 as long as gonocoxite, moderately pilose on basal 3/4. Parameres swollen (Fig. 9A) 1.3X as long as aedeagus, largely fused dorsally at base; apical portion with two process laterally directed, similar in length, one beak-shaped, the other hook-shaped. Aedeagus (Fig. 9B) triangular, composed of 2 pointed ventral plates, with slightly sclerotized dorsal structure, which arises in the middle way to aedeagus base and produced beyond the apices of ventral plates; basal arms slender, not expanded laterally. Female. Unknown Distribution. Brazil, Pará State. Type. Holotype male, on microscope slide labeled “Fazenda Morelândia (01°11’43”S, 48°15’35”W), Santa Bárbara do Pará, Pará, BRASIL, 21–22.IX.2008, CDC light trap, Igarapé Baiacú, Trindade R.L. & Guimarães D. cols. (MPEG). Etymology. This species is named based on the Muncipality of Santa Bárbara do Pará, where the specimen was collected. Taxonomic discussion. Monohelea barbara most closely resembles M. roraimensis Felippe-Bauer, M. gorayebi sp.nov. and M. visinensis sp.nov. by the brown legs with yellowish bands and by the presence of two apical processes in the paramere. However, one apical process of the paramere is beak-shaped and other hookshaped, similar in lenght, laterally directed (both processes are hook-shaped, one 2X longer than other, laterally directed in M. gorayebi; one process is beak-shaped, laterally directed, and other bilobed internally directed, different in length in M. roraimensis; one process is hook-shaped laterally directed, and other aculeated, anteroventraly directed in M. visinensis); by the basal arms of the aedeagus slender, not expanded laterally (basal arms large, greatly expanded laterally in M. gorayebi; basal arms slender, slightly expanded laterally in M. visinensis).Published as part of Felippe-Bauer, Maria Luiza, Cardoso, Erick Aragão & Trindade, Rosimeire Lopes Da, 2017, New species and new records of Monohelea Kieffer from eastern Amazon, Brazil (Diptera: Ceratopogonidae) in Zootaxa 4358 (1), DOI: 10.11646/zootaxa.4358.1.6, http://zenodo.org/record/106751

    Germinação de Stevia rebaudiana Bert

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    Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia

    Monohelea forceps Felippe-Bauer & Cardoso & Trindade 2017, sp. nov.

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    Monohelea forceps Felippe-Bauer & Trindade sp. nov. (Figs.1; 5 A–G; 9 G–H) Diagnosis. The only Neotropical species of Monohelea in which the males have yellowish legs, hind femur with an infuscated base, paramere single, swollen, curved, gradually tapering as a forceps, aedeagus with ventral plates abruptly tapered in middle portion. Female unknown. Male. Head: eyes widely separated (Fig. 5 C). Antenna missing. Palpus (Fig. 5 D) pale brown; 3rd segment nearly oval with small, moderately deep, sensory organ on mid length; lengths of segments 18- 28- 40- 25- 43; palpal ratio 2.0. Thorax. Without definite pattern on slide mounted specimen. Legs (Fig. 5 F) yellowish; fore-, mid coxae brown, hind coxae brown on apical 1/2; trochanters brown; fore-, mid femora with basal and mesal brown bands, hind femur with infuscated basal band, two brown stripes in middle, subapical ventral brown spot; fore tibia with inconspicuous mesal brown band, hind tibia with inconspicuous subbasal ventral brown spot, mesal brown stripe; apices of tibiae dark brown; hind tibial comb with 6 bristles. Tarsi: pale, except base of basitarsi pale brown; fore-,hind tarsomere 1 with one basal and one apical spine; midtarsomere 1 with 2 basal, 2 apical spines; apical spines of fore-, mid-, hind tarsomeres 2-4: 2-2-1, 2-2-2, 1-1-1, basal spines absent; fore, mid and hind tarsal ratios 2.15, 2.42, 2.00; claws small, paired, equal-sized, 0.4X as long as 5th tarsomeres. Wing (Fig. 5 A): macrotrichia restricted to vein costa; microtrichia absent; 2nd radial cell nearly 1.7X longer than 1st; wing length 0.97 mm, width 0.36 mm; costal ratio 0.79. Halter stem pale; knob pale brown in distal 1/2, a pale brown spot laterally (Fig. 5 B). Abdomen. Yellowish brown (Fig. 5 E). Genitalia (Figs. 5 G; 9 G–H): sternite IX spiculate except on basal portion, posterior margin with a short, convex, median lobe with 4 long setae; tergite IX tapering with a pair of apicolateral processes. Gonocoxite moderately stout, nearly 2X longer than basal width; gonostylus curved distally, 0.63X as long as gonocoxite, moderately pilose on basal 3/4. Parameres (Fig. 9 G) 1.15X as long as aedeagus, fused at single base; stem swollen, curved, gradually tapering, internally directed as a forceps. Aedeagus (Fig. 9 H) triangular, composed of 2 pointed ventral plates that abruptly tapered in middle portion, each with only delicately sclerotized apical projection which arises in the middle way to aedeagus base, and produced beyond the apices of ventral plates; basal arms slender, expanded laterally. Female. Unknown Distribution. Brazil, Pará State. Type. Holotype male, on microscope slide labeled “Vila Santa Maria” (01°01’45”S, 46°57’21”W), Tracuateua, Pará, BRASIL, 27–28.II.2007, CDC light trap, peridomicile, Gorayeb I. & Guimarães, D. cols. (MPEG). Etymology. The specific epithet is from the Latin: forceps = forceps, referring the stem of the parameres of males of this species. Taxonomic discussion. Monohelea forceps most closely resembles M. fairchildi Lane & Wirth, M. maya Felippe-Bauer, Huerta & Ibáñez-Bernal, M. patauateua sp. nov., and M. urracaisi Lane & Wirth by the single paramere without mesal or apical process. The new species can be easily separated by the hind femur with an infuscated base (hind femur mostly yellowish brown, without infuscated area on the base in M. fairchildi; hind femur with base uniformely brown in M. maya and M. patauateua; hind femur with pale base in M. urracaisi); by the paramere swollen, curved, gradually tapering as a forceps (paramere slender, sinuous in M. fairchildi; paramere straight, gradually tapering in M. maya; paramere slender,flattened in M. patauateua; paramere slender abruptly curved and tapering in the midlenght in M. urracaisi); by the ventral plates of the aedeagus that abruptly tapered in middle portion (ventral plates of the aedeagus gradually tapering in the other species).Published as part of Felippe-Bauer, Maria Luiza, Cardoso, Erick Aragão & Trindade, Rosimeire Lopes Da, 2017, New species and new records of Monohelea Kieffer from eastern Amazon, Brazil (Diptera: Ceratopogonidae) in Zootaxa 4358 (1), DOI: 10.11646/zootaxa.4358.1.6, http://zenodo.org/record/106751

    Monohelea ema Felippe-Bauer & Cardoso & Trindade 2017, sp. nov.

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    Monohelea ema Felippe-Bauer & Trindade sp. nov. (Figs. 1; 4 A–G; 9 E–F) Diagnosis. The only Neotropical species of Monohelea in which the males have hind femur with brown base, mid leg with distinct mesal brown bands, paramere with mesal hook-shaped process measuring 0.61X as long as distal portion that progressively narrows distally to rounded apex. Female unknown. Male. Head: eyes widely separated (Fig. 4 C). Antenna missing. Palpus (Fig. 4 D) pale brown; 3rd segment nearly oval with small, shallow, sensory organ on apical 1/3; lengths of segments 20- 25- 25- 25- 43; palpal ratio 1.25. Thorax. Brown; scutum damaged. Legs (Fig. 4 F) yellow; fore leg missing, mid-, hind coxae and trochanters brown; mid femur with basal and mesal pale brown bands, hind femur with basal brown band, one brown stripe in middle, a subapical ventral brown spot; mid tibiae with a mesal and apical pale brown band; hind tibia missing. Tarsi: mid tarsomere 1 with 2 basal, 2 apical spines; mid tarsomeres 2–4 with 2-2-2 apical spines, basal spines absent; mid tarsomere 5 missing; mid tarsal ratio 2.22; claws missing. Wing (Fig. 4 A) partially damaged; macrotrichia restricted to vein costa; microtrichia absent; 2nd radial cell nearly 2X longer than 1st; wing length nearly 0.90 mm, width 0.34 mm; costal ratio nearly 0.75. Halter stem pale; knob pale brown in distal 1/2, a pale brown spot laterally (Fig 4 B). Abdomen. Pale brown (Fig. 4 E). Genitalia (Figs. 4 G; 9 E–F): sternite IX spiculate except on basal portion, posterior margin with a short, convex, median lobe with 4 long setae; tergite IX tapering with a pair of apicolateral processes. Gonocoxite moderately stout, nearly 1.8 X longer than basal width; gonostylus curved in apical 1/2, 0.59 as long as gonocoxite, moderately pilose on basal 3/4. Parameres (Fig. 9 E) 1.36 X as long as aedeagus, broadly fused at trilobed base; each with a long, curved, sclerotized mesal hook-shaped process, 0.61X as long as distal portion of the paramere, that tapering gradually to blunt apex. Aedeagus (Fig. 9 F) triangular, composed of 2 pointed ventral plates, with slightly sclerotized dorsal structure, which arises in the middle way to aedeagus base, and produced beyond the apices of ventral plates, ending as an apical projection; basal arms slender, slightly expanded laterally. Female. Unknown Distribution. Brazil, Pará State. Type. Holotype male, on microscope slide labeled “Fazenda Ema (01°24’37”S, 46°22’12”W), Viseu, Pará, BRASIL, 23–24.VI.2007, CDC light trap, forest, Guimarães, D. col. (MPEG). Etymology. This species is named after the type locality. Taxonomic discussion. Characters for distinguishing Monohelea ema from the related species may be found in the discussion under the description of the M. cornuta.Published as part of Felippe-Bauer, Maria Luiza, Cardoso, Erick Aragão & Trindade, Rosimeire Lopes Da, 2017, New species and new records of Monohelea Kieffer from eastern Amazon, Brazil (Diptera: Ceratopogonidae) in Zootaxa 4358 (1), DOI: 10.11646/zootaxa.4358.1.6, http://zenodo.org/record/106751

    Monohelea cornuta Felippe-Bauer & Cardoso & Trindade 2017, sp. nov.

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    Monohelea cornuta Felippe-Bauer & Trindade sp. nov. (Figs. 1; 3 A–G; 9 C–D) Diagnosis. The only Neotropical species of Monohelea in which the males have yellowish legs, hind femur with infuscated base, mid leg without distinct mesal brown marks, paramere with a sclerotized mesal hook-shaped process, and distal portion of paramere truncated and overlapped. Female unknown. Male. Head: eyes widely separated (Fig. 3 C). Antenna missing. Palpus (Fig. 3 D) yellowish brown; 3rd segment nearly oval with small, shallow, sensory organ on apical 1/3; lengths of segments 15- 25- 30- 23- 38; palpal ratio 1.3-1.9 (1.6, n=2). Thorax. Brown; scutum without definite pattern on slide mounted specimens; scutellum yellowish laterally, with 4 bristles. Legs (Fig. 3 F) yellow; coxae and trochanters brown; fore femur with basal and mesal pale brown bands, mid femur with basal pale brown band, hind femur with infuscated basal band, two brown stripes in middle and a subapical ventral brown spot; apices of tibiae brown; hind tibia with additional dark irregular brown band on 1/2 subbasal; hind tibial comb with 6 bristles. Tarsi: pale; fore-, hind tarsomere 1 with one basal and one apical spine; midtarsomere 1 with 2 basal, 2 apical spines; apical spines of fore-, mid-, hind tarsomeres 2–4: 1-1-1 or 1-2- 2, 2-2-2, 1-1-1 or 1-1-2, basal spines absent; fore-, mid-, hind tarsal ratios 2.08–2.23 (2.17, n=4), 2.39–2.56 (2.47, n=4), 2.00 (n=3); claws small, paired, equal-sized, 0.3 to 0.5X as long as 5th tarsomeres. Wing (Fig. 3 A): macrotrichia restricted to vein costa; microtrichia absent; 2nd radial cell nearly 2.1X longer than 1st; wing length 0.78–0.85 (0.82, n= 5) mm, width 0.28–0.31 (0.30, n=5) mm; costal ratio 0.73–0.77 (0.75, n=5). Halter stem pale; knob pale brown in distal 1/2 a pale brown spot laterally (Fig. 3 B). Abdomen. Yellowish brown (Fig. 3 E). Genitalia (Figs. 3 G; 9 C–D): sternite IX spiculate except on basal portion, posterior margin with a short, convex, median lobe with 4 long setae; tergite IX tapering with a pair of apicolateral processes. Gonocoxite moderately stout, nearly 2X longer than basal width; gonostylus curved distally, 0.62–0.70 (0.66, n= 5)X as long as gonocoxite, moderately pilose on basal 3/4. Parameres (Fig. 9 C) 1.19–1.78 (1.44, n= 5)X as long as aedeagus, fused at base; each with an external curved, strongly sclerotized, mesal hornshaped process, 0.48–0.56 (0.52, n=5)X as long as distal portion of the paramere, that tapering to blunt apex, laterally truncated and overlapped. Aedeagus (Fig. 9 D) triangular, composed of 2 pointed ventral plates, with slightly sclerotized dorsal structure, which arises in the middle way to aedeagus base and produced beyond the apices of ventral plates, ending as an apical projection; basal arms slender, not expanded laterally. Female. Unknown Distribution. Brazil, Pará State. Types. Holotype male, on microscope slide labeled “ Vila de Curupaiti ” (01°25’56”S 46°28’16”W), Viseu, Pará, BRASIL, 20–21.VI.2007, CDC light trap, Trindade R.L. & Guimarães, D. cols. (MPEG). Paratypes 4 males: 3 males same data as holotype (2 CCER; 1 MPEG), 1 male same data as holotype except 22–23.VI.2007 (MPEG). Etymology. The specific epithet is from the Latin: cornu =horn, referring the mesal horn-shaped process on the parameres of males of this species. Taxonomic discussion. Monohelea cornuta most closely resembles M. aguirrei Tavares & Souza, M. archibaldoi Tavares & Souza, M. ema sp. nov., M. hieroglyphica Kieffer, M. maculipennis (Coquillett), M. poncai Lane & Wirth and M. uruguayensis Felippe-Bauer & Spinelli by the presence of a sclerotized mesal hook-shaped process. The new species can be easily separated by the infuscated base of the hind femur (hind femur with brown base in M. aguirrei, M. ema, M. poncai and M. uruguayensis; hind femur with pale base in M. archibaldoi and M. hieroglyphica), by the mid leg without distinct mesal brown marks as in M. archibaldoi (mid leg with mesal brown bands and spots in the other species), by the distal portion of the paramere, tapering to blunt apex, laterally truncated and overlapped (distal portion of the paramere progressively narrowing distally to rounded apex in M. aguirrei, M. archibaldoi, M. ema, M. maculipennis; distal portion of the paramere swollen, tapering and abruptly curved internally in M. hieroglyphica, M. poncai and M. uruguayensis).Published as part of Felippe-Bauer, Maria Luiza, Cardoso, Erick Aragão & Trindade, Rosimeire Lopes Da, 2017, New species and new records of Monohelea Kieffer from eastern Amazon, Brazil (Diptera: Ceratopogonidae) in Zootaxa 4358 (1), DOI: 10.11646/zootaxa.4358.1.6, http://zenodo.org/record/106751

    FIGURE 5 in New species and new records of Monohelea Kieffer from eastern Amazon, Brazil (Diptera: Ceratopogonidae)

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    FIGURE 5. Monohelea forceps Felippe-Bauer & Trindade sp. nov., male. A. Wing; B. Thorax showing halter; C. Head, anterior view; D. Palpus, lateral view; E. Abdomen, ventral view; F. Legs (left to right) of fore, mid-, hindlegs, lateral view; G. Genitalia, ventral view.Published as part of Felippe-Bauer, Maria Luiza, Cardoso, Erick Aragão & Trindade, Rosimeire Lopes Da, 2017, Zootaxa 4358 (1), DOI: 10.11646/zootaxa.4358.1.6, http://zenodo.org/record/106751
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