17 research outputs found
Development of Local Microorganism from Organic Waste as an Alternative Product for EM4
No full textResearch of Development Local Microorganism from Organic Waste by Adding Banana Peels as an Alternative Product for EM4 was conducted in the Laboratory Riset of Microbiology, Biology Department, Andalas University from February 2019 until July 2019. This study aims to find microbial groups and to determine the total amount of microbes in Local Microorganisms and to determine the quality of compost that produced by Local Microorganisms and EM4 as a control. This research design by survey method to sampling and experimental method to produced Local Microorganisms. The inoculation of bacteria colony was using Potato Dextro Agar (PDA) whereas inoculation of yeast colony using Malt Extract Agar (MEA). The result of this research showed that bacterial colonies on M4 2.8x 104 cfu/mL, fungi colonies 3.5 x 103 cfu/mL and yeast colonies 1.7 x 104 cfu/mL. The quality of compost produced has different color and texture however has the same in the aroma parameter
ANTIBACTERIAL POTENTIAL ETHANOL EXTRACT OF KAYU RACUN LEAF (Rhinacanthus nasutus) AGAINST Staphylococcus aureus AND METHICILLIN RESISTANT Staphylococcus aureus
No full textThe excessive use of antibiotics in the treatment of infections or diseases caused by Staphylococcus aureus is the main cause of antibiotic resistance. Methicillin-resistant S. aureus (MRSA) strains are a serious problem because of their wide distribution in the clinical environment and the living community, so it is necessary to search for antibacterial sources from natural products and traditional medicines such as Kayu racun leaves (Rhinacanthus nasutus). Information regarding the content of the active ingredient and the antibacterial potential of the ethanol extract of Kayu racun leaves is still limited. This study aims to test the phytochemical and antibacterial activity of the ethanol extract of Kayu racun leaves against S. aureus and MRSA bacteria by invitro. The research used an experimental method and was divided into two stages, namely; Phytochemical test of the ethanol extract of Kayu racun leaves using the Thin Layer Chromatography (TLC) method and the antibacterial activity test of the ethanol extract of Kayu racun leaves against S. aureus and MRSA using the paper disc diffusion method. Based on the research results, it can be concluded that the ethanol extract of Kayu racun leaves contains alkaloids, phenols, and flavonoids. The best antibacterial activity was found at a concentration of 100 mg/ml with an inhibition zone diameter of 26.20 mm against S. aureus and 17.90 mm against MRSA
Evaluasi Dan Analisis Pengelolaan Limbah Medis Padat Pada Fasilitas Kesehatan Tingkat Pertama Kota Padang
No full textMedical waste management procedures of health facilities such as the use of plastic bags that have not been in accordance with the type and characteristics of waste, the use of disinfection of medical waste containers that have been used, the lack of use of Personal Protective Equipment. In addition, the knowledge and attitude of medical waste management officers are still low such as the colour of plastic bags used, maximum storage of medical waste. This study used quantitative descriptive methods with a sample of 58 samples using the guttman scale and the likert scale with Multiple Regression analysis. For First Level Health Facilities (FKTP) most have carried out medical waste management in accordance with established regulations but there are some stages that are still low such as Temporary Shelter and Onsite Transportation. For knowledge (p = 0.031), attitude (p = 0.007) and perception of leadership support (p = 0.000) in medical waste management officers are still relatively low and the result of multiple regressions that knowledge, attitude and leadership support have a real effect on solid medical waste management. For FKTP as Health Centers and Clinics have carried out medical waste management by established standards but for the dentist practice is still low in the application of regulations regarding the management of solid medical waste.Tatacara Pengelolaan limbah medis fasilitas kesehatan seperti penggunaan kantong plastik yang belum sesuai dengan jenis dan karakteristik limbah, penggunaan desinfeksi terhadap wadah limbah medis yang telah digunakan, masih minimnya penggunaan Alat Pelindung Diri. Disamping itu pengetahuan dan sikap petugas pengelola limbah medis masih rendah seperti warna kantong plastik yang digunakan, penyimpanan maksimal limbah medis. Sehingga membutuhkan kontribusi pimpinan dalam pengelolaan limbah medis padat. Mengetahui dan menganalisis pengelolaan limbah medis padat di Fasilitas Kesehatan Tingkat Pertama Kota Padang. Penelitian ini menggunakan metode deskriptif kuantitatif dengan sampel sebesar 58 Sampel menggunakan skala guttman dan skala likert dengan analisis Regresi Berganda. Untuk Fasilitas Kesehatan Tingkat Pertama (FKTP) sebagian besar telah melaksanakan pengelolaan limbah medis sesuai dengan peraturan yang telah ditetapkan namun ada beberapa tahapan yang masih rendah seperti Tempat Penampungan Sementara dan Pengangkutan Onsite. Untuk pengetahuan (p = 0,031), sikap (p = 0,007) dan persepsi dukungan pimpinan (p = 0,000) pada petugas pengelola limbah medis masih tergolong rendah dan hasil regresi berganda bahwa pengetahuan, sikap dan dukungan pimpinan berpengaruh nyata terhadap pengelolaan limbah medis padat. Untuk FKTP seperti Puskesmas dan Klinik sudah melaksanakan pengelolaan limbah medis sesuai standar yang ditetapkan namun untuk praktek dokter gigi masih rendah dalam penerapan peraturan mengenai pengelolaan limbah medis pada
EKSPLORASI BAKTERI RESISTEN MERKURISEBAGAI BASIS KAJIAN BIOREMEDIASI LAHAN BEKAS TAMBANG EMAS
No full textKarakterisasi Bakteri Termofilik Penghasil Enzim Protease Netral
No full textA study on the characterization of thermophilic bacteria in producing neutral protease enzyme has been conducted in the Laboratory of Microbiology, Biology Department, Andalas University from October 2013 to March 2014. The bacteria were isolated from a hot spring at Sungai Medang, Kerinci. Water samples  were collected purposively based on gradient temperature at the hot spring which ranged from 450C to 880C. Colony of the bacteria were inoculated using nutrient agar (NA). We obtained 10 isolates of neutral protease enzyme from the thermophilic bacteria with proteolytic index ranged from 0.27 to 3.89. The MIV-14 isolate has the highest proteolytic index which collected from 830C of the hot spring. Characterization of macroscopic, microscopic and biochemical tests were reported. Generally, the colonies have round shape, wavy edge, white, producing endospores and motile. Keywords: characterization, neutral protease, thermophilic bacteria
Identifikasi Koleksi Jamur dari Cangkang dan Pasir Sarang Telur Penyu Lekang (Lepidochelys olivacea L.) di Penangkaran Pariaman
No full textThe district of Pariaman has a conservation site for sea turtles, including olive ridley (Lepidochelys olivacea L.). Several reports have stated that fungus infect egg of the turtles and caused the failure of hatching. However, there is no study on this site yet. The aim of this study was to identify fungus on turtle's egg, hatched and inhatched nest sand. Collection and identification were conducted from December 2012 until June 2013 in turtles’ breeding in Pariaman and Microbiology Laboratory of Biology Department of Mathematic and Natural Sience, Andalas University. Result showed that Fusarium sp. 1, Fusarium sp. 2, Fusarium sp. 3, Fusarium sp. 4, Fusarium sp. 5, Fusarium sp. 6, Fusarium sp. 7 dominated among identified fungus, other were Aspergillus sp. 1 and Trichoderma sp. 1 and than there was no fungus found on hatched egg.
Keywords: Identification, Fungus, Egg shell, Nest sand, Lepidochelys olivacea L
Isolasi dan Karakterisasi Bakteri pada Pasir Sarang dan Cangkang Telur Penyu Lekang (Lepidochelys olivaceae L.) yang Menetas dan Gagal Menetas
No full textIsolation and Characterization of Bacteria from nest sand and egg shell of Olive Ridley (Lepidochelys olivacea L.) which hatched and failed to hatch in Pariaman turtle conservation site, West Sumatra were conducted from December 2012 to September 2013 in Microbiology Laboratory, Andalas University and Laboratory of Provincial Health Department, West Sumatra. The aim of this study was to identify species of bacteria found at egg shells and nest sand of Olive Ridley in Pariaman. The result found 4 species of bacteria: Bacillus cereus and Klebsiella sp. from sand nest that hatched; while Bacillus cereus, Klebsiella sp. and Shigella sp. were identified from sand nest that failed to hatch. Bacteria isolates from egg shell that hatched were identified as Bacillus cereus, Klebsiella sp. While Bacillus cereus, Klebsiella sp., Salmonella sp. and Shigella sp. were identified from egg shell that failed to hatch. Keywords: isolation, bacteria, nest sand, egg shell, Olive Ridle
Screening of rhizobacteria from rhizosphere of healthy chili to control bacterial wilt disease and to promote growth and yield of chili
No full textFactors Associated With Public Behavior In Reducing Household Waste In Sungai Penuh City
No full textSungai Penuh City's waste is dominated by household waste at 40.76% with the largest composition of food waste at 44.76%. Based on Law No. 18/2008, one of the main focuses in waste management is reduction, but the practice of waste reduction in Sungai Penuh City has not been maximized. The study aims to analyze factors related to public behavior in waste reduction in Sungai Penuh City.The approach used was quantitative with survey techniques using questionnaires, which were carried out August-December 2023. The research subjects were 79 samples of people in Sungai Penuh City. The Chi Square test was used to determine the relationship of behavior to age, education, occupation, income, knowledge and attitude. The results of the Chi Square test show that there is a significant relationship between education and waste reduction behavior (P Value = 0.03), there is a significant relationship between employment and public behavior in waste reduction (P Value = 0.027), there is a significant relationship between knowledge and public behavior in waste reduction (P Value = 0.004), and there is a significant relationship between attitudes and public behavior in waste reduction in Sungai Penuh City (P Value = 0.000). while age has no significant relationship with public behavior in waste reduction (P Value = 0.581) and income has no significant relationship with public behavior in waste reduction in Sungai Penuh City
Analysis of Nutritional Content of Artificial Foods Made from Fermented Ficus racemosa Linn Flour
No full textFeed is a basic requirement in cultivating gourami fish. However, so far artificial feed has been quite expensive due to the soaring price of soybean flour. The high price of feed is an obstacle in fish farming. One effort to reduce feed costs is to make feed by substituting the basic ingredients of soybean flour with ingredients that are relatively cheap, but still contain high protein, such as fig flour. Efforts that can be made to optimize the absorption of feed nutrients are by improving the quality of feed, one of which is by adding feed additives in the form of probiotics to fish feed made from fermented fig flour. The aim of this research was to analyze the nutritional content of artificial feed made from fermented fig flour. This research uses an experimental method by fermenting artificial feed made from fig flour using EM4. The research results showed that the nutritional content of artificial feed made from fermented fig flour was obtained respectively; Water content 6.81% (g/100g), Ash content 9.94 % (g/100g), Crude Protein (N X 6.25) 40.02 % (g/100g), Crude Fat 7.16 % (g /100g) carbohydrates 36.03 % (g/100g) and Crude Fiber 2.883 % (g/100g)
