12,510 research outputs found
Ms. Courtney Chartier, RWWL AUC, August 2011
No full textThis video is a conversation with Ms. Courtney Chartier. Ms. Chartier talks about her work on the "New Georgia Encyclopedia" and "Online Voter Education Project." Andrea Jackson, AUC Woodruff Library, is the interviewer
Ms. Neely Terrell, RWWL AUC, March 2012
No full textThis video is a conversation with Ms. Neely Terrell. Ms. Terrell talks about her book, "Super Singles Activate". Anthony Kinsey and Jahnesta Horney, AUC Woodruff Library, are the interviewers
Ms. Felesha Love, Spelman College, January 2016
No full textThis video is a conversation with Felesha Love. Ms. Love talks about her book, "Brave Leap to Freedom: Integrating Mind, Body, and Spirit to Cultivate Healthy Relationships". Jordan Moore, AUC Woodruff Library, is the interviewer
Étude sur le patois de Valbonnais
No full textA lexical and morphologic description of Valbonnais dialect. A 319-page PhD dissertation under the direction of Prof. Antonin DURAFFOUR (Univ. Stendhal, Grenoble, France, 1943)Description lexicale et morphologique du patois de Valbonnais sous la forme d'un manuscrit de 319 pages.Thèse sous la direction du Prof. Antonin DURAFFOUR (Univ. Stendhal, Grenoble, 1943
A Method for Analysis of Free and Total Ropivacaine in Dog Plasma Using UHPLC-MS/MS
No full textRopivacaine is a local anesthetic commonly used in veterinary anesthesia. A liquid chromatography–mass spectrometry (LC–MS) method was developed to quantify free and total ropivacaine in dog plasma, which included rapid equilibrium dialysis. The method was validated for selectivity, specificity, matrix effect, calibration curve and range, accuracy and precision, carry-over, stability, and reinjection reproducibility according to the International Conference on Harmonization M10 guidelines. After ultra-high performance liquid chromatographic (UHPLC) separation, detection and quantification of ropivacaine was performed using a triple quadrupole tandem mass spectrometer with electrospray ionization. LC–MS method validation was carried out in a range of 0.05–1000 ng/mL ropivacaine in dog plasma in two dilutions (1:1 and 1:4). The precision and accuracy of the method were determined at four concentration levels and ranged from 0.40% to 5.30% and 85.50% to 113.30%, respectively. The lower limit of quantification was as low as 0.30 and 0.05 ng/mL, for the quantitation of protein-bound (1:4) and free (1:1) ropivacaine, respectively. All validation parameters met acceptance criteria. This UHPLC–MS/MS method was successfully applied in a clinical study that involved the intraperitoneal instillation of ropivacaine to anesthetized dogs and can be used to quantify free and total ropivacaine in dog plasma
Desenvolvimento de métodos para multi-toxinas por cromatografia líquida de alta eficiência acoplada à espectrômetro de massa/massa
No full textDissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências Agrárias. Programa de Pós-Graduação em Ciência dos Alimentos.Métodos utilizando cromatografia líquida de alta eficiência acoplada à espectrômetro de massa/massa (LC-MS/MS) foram desenvolvidos para diferentes grupos de micotoxinas e avaliados em castanha-do-Brasil. (a) Desenvolvimento de um Método para Determinação de Aflatoxinas B1, B2, G1, e G2 por LC-MS/MS em castanhas-do-Brasil. O primeiro método foi desenvolvido utilizando ionização química à pressão atmosférica (APCI) no modo positivo [M+H]+, para determinação simultânea das aflatoxinas (AFLs) B1, B2, G1, e G2 em castanhas-do-Brasil. Através da monitoração de múltiplas reações foi possível analisar as transições das AFLs aumentando assim a especificidade e sensibilidade. A separação das AFLs foi realizada utilizando uma coluna C8 e um gradiente de fase móvel composta por água:metanol (ambos com 25 mM de acetato de amônia) em um tempo total de corrida de 7 minutos. A extração das toxinas foi conduzida utilizando uma solução de acetonitrila:água (80:20) e não foi utilizada etapa de limpeza. Os valores de limite de detecção (LOD) e de quantificação (LOQ) do método para AFB1, AFB2, AFG1 e AFG2 foram 0.04; 0.045; 0.050 e 0.060 µg.kg-1 e 0.08, 0.09, 0.10 e 0.12 µg.kg-1 respectivamente. Os coeficientes de correlação para as toxinas foram excelentes e a linearidade foi obtida em uma faixa de 0.005, 0.02 0.25 e 0.04 até 0.5, 2.0, 25 e 4.0 µg.kg-1 para cada toxina respectivamente. Os componentes da matriz não influenciaram a detecção e quantificação das AFLs. Os valores de recuperação ficaram entre 92 e 100 % e valor do AFLs obtidos a partir de amostras naturalmente contaminadas esteve entre 1.2 à 11.5 µg.kg-1. (b) Desenvolvimento de Métodos para 15 Micotoxinas por LC-MS/MS Utilizando Ionização Química por Pressão Atmosférica e por Eletrospray. Neste estudo dois métodos foram desenvolvidos para quantificação de 15 micotoxinas APCI e eletrospray (ESI) como fontes de ionização. A separação foi realizada utilizando uma coluna C18 e gradientes de fase móvel compostas de metanol:água (ambos com 25 mM e acetato de amônia). As toxinas estudadas foram AFLs, citrinina (CTR), fumonisinas (FB1 e FB2), ocratoxina A (OTA), tricotecenos (NEO; 15ADON; 3ADON; DAS; HT2; T2) e zearalenona (ZON). Os tempos cromatográficos para os métodos foram de 11.5 e 9.5 minutos para APCI e ESI respectivamente. APCI não foi capaz de ionizar FB1; FB2; CTR e OTA e a ESI não foi capaz de ionizar as AFLs. Os LOQs utilizando APCI foram 0.25, 2.5, 0.7, 0.01, 0.015, 0.015, 0.02, 0.2, 1.0, HT2, T2 e ZON respectivamente. Utilizando ESI, os LOQs foram de 0.1, 1.5, 0.14, 0.04, 0.08, 0.1, 0.2, 0.08, 2.5, 0.08 e 0.05 µg.kg-1 para NEO; 15ADON; 3ADON; DAS; HT2; T2; FB1; FB2; CTR; OTA e ZON respectivamente. Sete micotoxinas foram ionizadas pelas duas fontes de ionização. Todavia, os LOQs foram menores utilizando ESI. Os dois métodos desenvolvidos mostraram vantagens e desvantagens quando comparados e a escolha da fonte de ionização dependerá do objetivo do estudo. Os dois estudos confirmaram o uso da espectrometria de massas como uma das melhores ferramentas para análise de micotoxinas em alimentos, especialmente devido às restrições das legislações internacionais
Radar Chart of Citation topic Meso, source: Formulated by author using MS Excel (2016).
No full textRadar Chart of Citation topic Meso, source: Formulated by author using MS Excel (2016).</p
Improving MHC-I ligand identifications from LC-MS/MS data by incorporating allelic peptide motifs
No full textMHC class I (MHC-I)-bound ligands play a pivotal role in CD8 T cell immunity and are hence of major interest in understanding and designing immunotherapies. One of the most commonly utilized approaches for detecting MHC ligands is LC-MS/MS. Unfortunately, the effectiveness of current algorithms to identify MHC ligands from LC-MS/MS data is limited because the search algorithms used were originally developed for proteomics approaches detecting tryptic peptides. Consequently, the analysis often results in inflated false discovery rate (FDR) statistics and an overall decrease in the number of peptides that pass FDR filters. Andreatta et al. describe a new scoring tool (MS-rescue) for peptides from MHC-I immunopeptidome datasets. MS-rescue incorporates the existence of MHC-I peptide motifs to rescore peptides from ligandome data. The tool is demonstrated here using peptides assigned from LC-MS/MS data with PEAKs software but can be deployed on data from any search algorithm. This new approach increased the number of peptides identified by up to 20-30% and promises to aid the discovery of novel MHC-I ligands with immunotherapeutic potential
Characterization of Biological Components of Leaves and Flowers in Moringa peregrina and Their Effect on Proliferation of Staurogyne repens in Tissue Culture Conditions
No full textMoringa peregrina (Forssk.) Fiori is a tropical tree in southern Iran known as the most important natural coagulant in the world. Today, plant tissue culture is a new method that has a very high potential to produce valuable medicinal compounds on a commercial level. Advances in in vitro cultivation methods have increased the usefulness of plants as renewable resources. In this study, in addition to the phytochemical analysis of the extract of M. peregrina using HPLC, the interaction effect of different concentrations of aqueous extract of M. peregrina (0, 1, 1.5, and 3 mg/L) in two types of MS and 1⁄2 MS basal culture media over three weeks on the in vitro growth of Staurogyne repens (Nees) Kuntze was studied. The amounts of quercetin, gallic acid, caffeic acid, and myricetin in the aqueous extract of M. peregrina were 64.9, 374.8, 42, and 4.6 mg/g, respectively. The results showed that using M. peregrina leaf aqueous extract had a positive effect on the length of the branches, the percentage of green leaves, rooting, and the fresh and dry weight of S. repens samples. The highest increase in growth indices was observed in the MS culture medium supplemented with 3 mg/L of M. peregrina leaf aqueous extract after three weeks of cultivation. Of course, this effect was significantly greater in the MS medium and at higher concentrations compared to the 1⁄2 MS medium. Three weeks after cultivation at a concentration of 3 mg/L of the extract, the length of the S. repens branches was 5.3 and 1.8 cm in the two basic MS and 1⁄2 MS culture media, and the percentage of green leaves was 14 and 4 percent, respectively. Also, rooting was measured at 9.6 and 3.6 percent, fresh weight at 6 and 1.4 g, and dry weight at 1.1 and 0.03 g, respectively. Therefore, adding M. peregrina leaf aqueous extract as a stimulant significantly increased the in vitro growth of S. repens
Collecting Cures in an Artisanal Manuscript: Practical Therapeutics and Disease in Ms. Fr. 640
No full textScattered throughout Ms. Fr. 640, the forty medical recipes form a small percentage of its over 900 entries. A consideration of the ailments, ingredients, and making processes described in the manuscript, as well as the author-practitioner’s process of collecting information, reveals a variety of connections between Ms. Fr. 640’s medical recipes and early modern artisanal work
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