444 research outputs found
Enzymatic degradation of polyethylene terephthalate (PET): use of bacterial Ideonella sakaiensis PETase enzyme in Escherichia coli BL2 as a Bioremediation tool.
No full textBioremediation is the use of microorganism to consume or break down pollutants in the natural environment. This thesis focuses his study on the PET (Polyethylene terephthalate) pollution and its enzymatic degradation due to PETase enzyme activity produce by Ideonella sakaiensis 201-F6. These aerobic bacteria shown to produce two specific enzymes, IsPETase and MHETase, that allow him to use the PET as a carbon source and brake down the hydrocarbon chain.
The research project aimed to study the expression of wild type IsPETase (WT) and a mutant of the WT enzyme, IsPETase W159H-S238F (M) expressed in Escherichia Coli BL21(D3). These two enzymes were subjected to the same treatment, and samples were taken every 24h and 48h for protein analysis and PET degradation rate quantification. Secondly, with a bioinformatics approach, we aimed to suggest a possible novel mutant of the WT IsPETase that would show high degradative capacity and high enzyme expression in bacterial periplasm. This type of study involved the use of different bioinformatics tool, such us: BLAST, CLUSTAL, Pymol and CHARMM-GUI
Hydrophobically modified chitosan nanoliposomes for intestinal drug delivery
No full textM Gulrez Zariwala,1 Harshada Bendre,2 Anatoliy Markiv,3 Sebastien Farnaud,4 Derek Renshaw,4 Kevin MG Taylor,2 Satyanarayana Somavarapu2 1Faculty of Science and Technology, University of Westminster, London, UK; 2Department of Pharmaceutics, University College London School of Pharmacy, London, UK; 3Faculty of Life Sciences and Medicine, King’s College London, London, UK; 4Faculty of Health and Life Sciences, Coventry University, Coventry, UK Background: Encapsulation of hydrophilic drugs within liposomes can be challenging. Methods: A novel chitosan derivative, O-palmitoyl chitosan (OPC) was synthesized from chitosan and palmitoyl chloride using methane-sulfonic acid as a solvent. The success of synthesis was confirmed by Fourier transform infra-red (FT-IR) spectroscopy and proton NMR spectroscopy (H-NMR). Liposomes encapsulating ferrous sulphate as a model hydrophilic drug for intestinal delivery were prepared with or without OPC inclusion (Lipo-Fe and OPC-Lipo-Fe). Results: Entrapment of iron was significantly higher in OPC containing liposomes compared to controls. Quantitative iron absorption from the OPC liposomes was significantly higher (1.5-fold P<0.05) than free ferrous sulphate controls. Qualitative uptake analysis by confocal imaging using coumarin-6 dye loaded liposomes also indicated higher cellular uptake and internalization of the OPC-containing liposomes. Conclusion: These findings suggest that addition of OPC during liposome preparation creates robust vesicles that have improved mucoadhesive and absorption enhancing properties. The chitosan derivative OPC therefore provides a novel alternative for formulation of delivery vehicles targeting intestinal absorption. Keywords: gut delivery, intestinal absorption, Caco-2, ferrous sulfat
Sebastien Rale vs. New England: A Case Study of Frontier Conflict
Full text linkAuthor\u27s original abstract: A study was made of the Jesuit missionary, Sebastien Rale, and his role in New England-New France relations. French and English primary and secondary materials were examined to give the broadest possible view of the man and to place him in historical context.
It was found that Sebastien Rale was not an agent of New France. The conflicting opinions surrounding the mission of Norridgewock and the border war of the 1720\u27s were traced to the problems of Massachusetts-Abnaki relations. Rale\u27s frequent and testy letters to the government of the Bay Colony were blunt reactions to what he viewed as religious and territorial threats against his mission.
The frontier conflict between 1713 and 1722 was not the result of French Imperial policy. The French insisted that the Abnakis were allies but refused active participation in the Indians\u27 quarrel with New England. Policy was developed in Maine by the Jesuits. The missionaries were only secondarily interested in Quebec\u27s desire to prevent Massachusetts\u27 settlement of the Kennebec. With the declaration of war in July, 1722, however, the Jesuits left the Abnakis in the hands of the governor and the intendant of New France on whom the Indians relied for vital war supplies.
Finally, the controversial attack on Norridgewock was appraised. It was found that no secondary account had fully evaluated the sources. Examination led to the discovery of crucial inconsistencies in the primary accounts of New England. The French sources were found to be based on the understandably confused impressions of the fleeing Indians. In large measure the English sources present the more valid picture: the sudden attack, the panicked confusion, and Sebastien Rale dying with gun in hand. After Rale\u27s death the war drew to a close. Without Sebastien Rale\u27s persuasion and determination, the Abnakis were not able to present a united front against colonial expansion
Steve J. Chavez recital thesis 2012
No full textThesis (M.M.)
California State University, Los Angeles, 2012Committee members: Sebastien Vallee, Jeffrey Benedict, John KennedyThe musical selections from this Master recital were not chosen with a specific theme in mind, rather each selection was carefully selected to represent the requisite musical styles stemming from the 18th to 20th century.All the literature was selected based on the requirements of the music department representing the technical skill level worthy of a graduate student from the institution of California State University, Los Angeles. This written project is the presentation of historical and musical research gathered by this author in preparation for this recital. The selections of Bach, Handel, Beethoven, Schubert, Faure, Menotti, Hoiby, and Barber are discussed in turn
Considerations sur l'ordre de Cincinnatus, [electronic resource] : ou imitation d'un pamphlet anglo-americain. Par le comte de Mirabeau. Suivies de plusieurs pieces relatives a cette institution; ... d'un pamphlet du Docteur Price, intitule: Observations on the importance of the American Revolution, ...
No full textSebastien Roch Nicholas Chamfort is given as joint author by Querard and Barbier.A reissue of the 1784 Londres edition, with a different title-page.Sig. H1 is a cancel - The 'Avis' is dated: Londres, 20 septembre, 1784.Printer's name from colophon.Electronic reproduction.English Short Title Catalog,Reproduction of original from British Library
Limit Theorem for a Modified Leland Hedging Strategy under Constant Transaction Costs rate
Full text linkWe study the Leland model for hedging portfolios in the presence of a constant proportional transaction costs coefficient. The modified Leland's strategy recently defined by the second author, contrarily to the classical one, ensures the asymptotic replication of a large class of payoff. In this setting, we prove a limit theorem for the deviation between the real portfolio and the payoff. As Pergamenshchikov did in the framework of the usual Leland's strategy, we identify the rate of convergence and the associated limit distribution. This rate turns out to be improved using the modified strategy and non periodic revision dates.Asymptotic hedging ; Leland-Lott strategy ; Transaction costs ; Martingale limit theorem.
Going Serverless with Knative on Kubernetes
No full textAbstract:
After reviewing some of the latest evolutions in Kubernetes we will discuss the serverless computing paradigm and highlight a few application use-cases. We will then introduce Knative, a system built on top of Kubernetes which provides components to build and serve applications as well as manage events. Through quick demos will demonstrate several key capabilities like scaling to zero and also highlight future directions with service mesh technologies.
Bio:
Sebastien is the co-founder of TriggerMesh (https://triggermesh.com) a serverless management platform built on Knative. He is the author of the Docker and Kubernetes O'Reilly cookbooks and was the CMS Tier-2 site lead at Purdue University more than 10 years ago
GALDIERO is CO-FIRST AUTHOR. Phagocytes as Corrupted Policemen in Cancer-Related Inflammation
No full textInflammation is a key component of the tumor microenvironment. Tumor-associated macrophages (TAMs) and tumor-associated neutrophils (TANs) are prototypic inflammatory cells in cancer-related inflammation. Macrophages provide a first line of resistance against infectious agents but in the ecological niche of cancer behave as corrupted policemen. TAMs promote tumor growth and metastasis by direct interactions with cancer cells, including cancer stem cells, as well as by promoting angiogenesis and tissue remodeling and suppressing effective adaptive immunity. In addition, the efficacy of chemotherapy, radiotherapy, and checkpoint blockade inhibitors is profoundly affected by regulation of TAMs. In particular, TAMs can protect and rescue tumor cells from cytotoxic therapy by orchestrating a misguided tissue repair response. Following extensive preclinical studies, there is now proof of concept that targeting tumor-promoting macrophages by diverse strategies (e.g., Trabectedin, anti-colony-stimulating factor-1 receptor antibodies) can result in antitumor activity in human cancer and further studies are ongoing. Neutrophils have long been overlooked as a minor component of the tumor microenvironment, but there is evidence for an important role of TANs in tumor progression. Targeting phagocytes (TAMs and TANs) as corrupted policemen in cancer may pave the way to innovative therapeutic strategies complementing cytoreductive therapies and immunotherapy
Corrigendum to “Reliability assessment of generic geared wind turbines by GTST-MLD model and Monte Carlo simulation” (Renewable Energy (2015) 83 (222–233), (S0960148115003158), (10.1016/j.renene.2015.04.035))
No full textThe authors regret that the Order of Authors in this article published in November 2015 is incorrect. Thus, the objective of this Corrigendum is to re-establish the originally agreed Order of Authors, as described below. Order of Authors from published Article: Yan-Fu Li, PhD; Sebastien Valla; Enrico Zio, PhD. Corrected Order of Authors to implement with this Corrigendum: Sebastien Valla, Yan-Fu Li, PhD; Enrico Zio, PhD. The Corresponding author to contact for these changes are the Primary Author, Sebastien Valla (email below). The authors would like to apologise for any inconvenience caused
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