5 research outputs found
Relationship between phase angle measured with bioelectrical impedance analysis and nonalcoholic fatty liver disease in adults
No full textPurpose - The phase angle (PhA) measured by bioelectrical impedance analysis (BIA) is associated with nutritional status and cellular health, and it is a clinically important parameter used to assess the risk of various diseases. It remains unclear whether PhA is associated with nonalcoholic fatty liver. The purpose of this study is to investigate the relationship between the BIA parameter PhA and nonalcoholic fatty liver disease (NAFLD). Design/methodology/approach - This cross-sectional study was conducted with 300 adults aged 20-64 years (NAFLD: 196, normal: 104). Some biochemical findings of the participants were collected, and whole-body and segmental PhAs were measured using Tanita (MC-780) at 50 kHz. Findings - The PhA values of the whole body (p = 0.003), trunk (p < 0.001), right and left legs (p < 0.001 for both) were found to significantly differ according to the degrees of fatty liver. It was observed that the highest PhA values were in normal individuals. Logistic regression analysis showed that the reduction in PhAs of the whole body (p = 0.038), right (p = 0.019) and left legs (p = 0.049) and trunk (p = 0.001) after adjusting for all confounding factors increased the risk of NAFLD. Additionally, whole body PhA was significantly associated with age (year, p = 0.02), BMI (kg/m2; p < 0.001), fat mass (kg; p = 0.001), fat mass (%; p < 0.001), albumin (g/L; p < 0.001) and CRP (mg/dL; p = 0.001). Originality/value - The results of this study showed that PhA can be used in the management of NAFLD. To identify potential mechanisms in the relationship between the angle of the liver and NAFLD, large-scale prospective studies are needed.The authors are grateful to all individuals who participated in this study. Thanks to ChatGPT 4.0, which was used to improve language and readability in some sentences of this study. Funding: The author declares that no funds, grants or other support were received during the preparation of this manuscript. Authors' contributions: Conceptualization: Mustafa Capraz; Methodology: Cansu Memic nan, Mustafa Capraz; Formal analysis: Cansu Memic nan; investigation: Cansu Memic nan; writing - original draft: Cansu Memic nan; supervision: Mustafa Capraz. Availability of data and materials: The data sets used and/or analyzed during the current study are available from the corresponding author upon reasonable request. Declaration of conflicting interests: The authors declared no potential conflicts of interest with respect to the research, authorship and/or publication of this study. Ethical statements approval: Informed written consent was obtained from all participants prior to data collection. Ethics Committee Permission was obtained from Amasya University for this study (No. 050.01.04-102553). All study procedures were conducted according to the guidelines of the Declaration of Helsinki
The influence of projectile impact velocity and target characteristics on the terminal ballistics parameters of small-caliber projectile
No full textThe study examines the results of numerical simulations of the penetration of a 7,62mm x 63, AP, M2 projectile through a target. Model validation involved a numerical simulation of 7,62mm x 63, AP, M2 projectile penetration through an AA5083H116 target, for which experimental results were available in the literature. The results of the numerical simulation were used for regression analysis - to calibrate the Recht-Ipson model for the given projectile. Furthermore, penetration analyses of 7,62mm x 63, AP, M2 projectiles through various target materials were performed at consistent projectile velocities and mesh sizes. As expected, bainitic steels exhibited the highest resilience, demonstrating that increased material hardness and tensile strength correlate with enhanced projectile penetration resistance
Kartläggning av reparationsprocessen på Westermo Teleindustri AB : Identifiering av slöserier och förslag till förbättringar
No full textThis report is the result of a thesis performed at Westermo Industrial Ltd with its headquarters in Stora Sundby and it sums up the author's studies for University Engineering in Innovation, Production and Logistics at Mälardalen University. The main goal of the thesis was to identify problems and suggest actions that lead to the reduction of the repair time from 3 weeks to 2 weeks. The key questions that were answered in order to achieve the thesis objectives are: What are the biggest challenges that make the repair process time last for 3 weeks? What are the necessary means and measures in order to reduce the repair length to 2 weeks? The author adjusted the work to the Swedish repairs, repair issues that arise from sell unit Westermo Data Communications AB with its office in Västerås and performed the status report by mapping the repair process, through using observations, interviews and bibliography research studies. A flow map of four sub-processes (Inbound Delivery, BackOffice, Repair Department, and Logistics) was made and each section was observed, where also the employees were interviewed. Data that has been collected was analysed by the author and the decision to execute a workshop that affects Repair Department and Logistics was made. Consequently to this, these two services will prove to have the greatest impact on repair time. It is notified that the main reason for the repair process length today is so long is due to the fact that the owner of the repair process does not exist, which leads to so many unanswered questions and unclearness through the whole process, which occur and result in the situation where the process does not work as a whole, but each sub-process is acting separately on its own. Problems also arise because of the persons that are bound to the process, where both engineers at repair department are specialized in their own field and cannot perform the second engineer's work. Furthermore, due to the incomplete description that is usually provided by the customers, makes even more difficult to define the problem, which is yet another reason why the repair time is so long today. The suggestions for improvement that are recommended, in the first place to reduce the length of the repair process - is to create an entirely new position within repair process – the owner. In order to correct the deficiency with incomplete error descriptions from the clients, they should introduce network-based fault reports, which contain a number of mandatory fields that the customer must fill out in order to describe the problem and the conditions that existed when the fault with their unit occurred. This would simultaneously also eliminate the waiting length on the warranty of the unit if it is not applicable, and a response regarding that particular repair for the customer. Furthermore, training of repair engineers should be initiated for them, so they will be able to fix all units coming in for repair. The key objective here is to reduce the waiting length when the unit is on wait to be treated by the "right" engineer. In future, with the aforementioned, and other improvement proposals that are developed and explained in this report, repair time can be significantly reduced, which will further on improve the entire repair process
Microfluidic techniques for development of 3D vascularized tissue
No full textDevelopment of a vascularized tissue is one of the key challenges for the successful clinical application of tissue engineered constructs. Despite the significant efforts over the last few decades, establishing a gold standard to develop three dimensional (3D) vascularized tissues has still remained far from reality. Recent advances in the application of microfluidic platforms to the field of tissue engineering have greatly accelerated the progress toward the development of viable vascularized tissue constructs. Numerous techniques have emerged to induce the formation of vascular structure within tissues which can be broadly classified into two distinct categories, namely (1) prevascularization-based techniques and (2) vasculogenesis and angiogenesis-based techniques. This review presents an overview of the recent advancements in the vascularization techniques using both approaches for generating 3D vascular structure on microfluidic platforms. © 2014 Elsevier Ltd.Adams RH, 2007, NAT REV MOL CELL BIO, V8, P464, DOI 10.1038-nrm2183; Anderson DEJ, 2011, ANN BIOMED ENG, V39, P2329, DOI 10.1007-s10439-011-0352-z; ARCHIPOFF G, 1993, BRIT J PHARMACOL, V109, P18; Assal Y, 2013, BIOMATERIALS, V34, P3315, DOI 10.1016-j.biomaterials.2013.01.067; Bae H, 2012, SCI TRANSL MED, V4, P1; Barkefors I, 2008, J BIOL CHEM, V283, P13905, DOI 10.1074-jbc.M704917200; Bergers G, 2005, NEURO-ONCOLOGY, V7, P452, DOI 10.1215-S1152851705000232; Bettinger CJ, 2007, ADV MATER, V19, P2847, DOI 10.1002-adma.200602487; Bettinger CJ, 2006, ADV MATER, V18, P165, DOI 10.1002-adma.200500438; Bettinger CJ, 2008, ADV MATER, V20, P99, DOI 10.1002-adma.200702487; Borenstein JT, 2002, BIOMED MICRODEVICES, V4, P167, DOI 10.1023-A:1016040212127; Boyd NL, 2011, TISSUE ENG PT A, V17, P1537, DOI [10.1089-ten.TEA.2010.0397, 10.1089-ten.tea.2010.0397]; Butcher JT, 2006, ARTERIOSCL THROM VAS, V26, P69, DOI 10.1161-01.ATV.0000196624.70507.0d; Butcher JT, 2006, TISSUE ENG, V12, P905, DOI 10.1089-ten.2006.12.905; Cabodi M, 2005, J AM CHEM SOC, V127, P13788, DOI 10.1021-ja054820t; Campbell PG, 2007, EXPERT OPIN BIOL TH, V7, P1123, DOI 10.1517-14712598.7.8.1123; Carmeliet P, 2011, NATURE, V473, P298, DOI 10.1038-nature10144; Chaw KC, 2007, BIOMED MICRODEVICES, V9, P597, DOI 10.1007-s10544-007-9071-5; Chen MB, 2013, INTEGR BIOL-UK, V5, P1262, DOI 10.1039-c3ib40149a; Chen YC, 2012, ADV FUNCT MATER, V22, P2027, DOI 10.1002-adfm.201101662; Chiu LLY, 2010, BIOMATERIALS, V31, P226, DOI 10.1016-j.biomaterials.2009.09.039; Choi NW, 2007, NAT MATER, V6, P908, DOI 10.1038-nmat2022; Chrobak KM, 2006, MICROVASC RES, V71, P185, DOI 10.1016-j.mvr.2006.02.005; Cross VL, 2010, BIOMATERIALS, V31, P8596, DOI 10.1016-j.biomaterials.2010.07.072; Cuchiara MP, 2012, ADV FUNCT MATER, V22, P4511, DOI 10.1002-adfm.201200976; Culver JC, 2012, ADV MATER, V24, P2344, DOI 10.1002-adma.201200395; Daamen WF, 2007, BIOMATERIALS, V28, P4378, DOI 10.1016-j.biomaterials.2007.06.025; Davies PF, 2008, NAT CLIN PRACT CARD, V6, P16; Dejana E, 2004, NAT REV MOL CELL BIO, V5, P261, DOI 10.1038-nrm1357; Deutsch M, 2009, J VASC SURG, V49, P352, DOI 10.1016-j.jvs.2008.08.101; Dickinson LE, 2010, SOFT MATTER, V6, P5109, DOI 10.1039-c0sm00233j; Du Y, 2008, MICROFLUIDICS BIOL A; Du YA, 2008, P NATL ACAD SCI USA, V105, P9522, DOI 10.1073-pnas.0801866105; Epstein FH, 1990, NEW ENGL J MED, V323, P27, DOI 10.1056-NEJM199007053230106; Fidkowski C, 2005, TISSUE ENG, V11, P302, DOI 10.1089-ten.2005.11.302; Fischer C., 2006, HDB EXP PHARM, P157, DOI DOI 10.1007-3-540-36028-X_6); FUNG YC, 1995, P NATL ACAD SCI USA, V92, P2169, DOI 10.1073-pnas.92.6.2169; Gailloud P, 1999, AM J NEURORADIOL, V20, P291; Golden AP, 2007, LAB CHIP, V7, P720, DOI 10.1039-b618409j; Greenwald SE, 2000, J PATHOL, V190, P292, DOI 10.1002-(SICI)1096-9896(200002)190:3292::AID-PATH5283.0.CO;2-S; Gu W, 2004, P NATL ACAD SCI USA, V101, P15861, DOI 10.1073-pnas.0404353101; Hahn MS, 2006, BIOMATERIALS, V27, P2519, DOI 10.1016-j.biomaterials.2005.11.045; Hansen NS, 2011, NANOTECHNOLOGY, V22, DOI 10.1088-0957-4484-22-32-325302; Hao XJ, 2007, CARDIOVASC RES, V75, P178, DOI 10.1016-j.cardiores.2007.03.028; Hasan A, 2014, J BIOMECH IN PRESS; Hasan A, 2013, ACTA BIOMATER, V10, P11; He JK, 2013, BIOFABRICATION, V5, DOI 10.1088-1758-5082-5-2-025002; He JK, 2013, MATER LETT, V90, P93, DOI 10.1016-j.matlet.2012.09.024; Helm CLE, 2005, P NATL ACAD SCI USA, V102, P15779, DOI 10.1073-pnas.0503681102; Hendrix MJC, 2003, NAT REV CANCER, V3, P411, DOI 10.1038-nrc1092; Heydarkhan-Hagvall S, 2006, TISSUE ENG, V12, P831, DOI 10.1089-ten.2006.12.831; Holzapfel GA, 2002, EUR J MECH A-SOLID, V21, P441, DOI 10.1016-S0997-7538(01)01206-2; Holzapfel GA, 2001, HDB MAT BEHAV MODELS; Hopkins SP, 1998, J VASC SURG, V27, P886, DOI 10.1016-S0741-5214(98)70269-1; Hsu YH, 2013, LAB CHIP, V13, P2990, DOI 10.1039-c3lc50424g; Huang CP, 2009, LAB CHIP, V9, P1740, DOI 10.1039-b818401a; Inoguchi H, 2007, BIOMATERIALS, V28, P486, DOI 10.1016-j.biomaterials.2006.09.020; Intengan HD, 2000, HYPERTENSION, V36, P312; Isner JM, 1999, J CLIN INVEST, V103, P1231, DOI 10.1172-JCI6889; Ito Y, 2013, BIOMATERIALS, V34, P7565, DOI 10.1016-j.biomaterials.2013.06.037; Jakab K, 2010, BIOFABRICATION, V2, DOI 10.1088-1758-5082-2-2-022001; Jakab K, 2004, P NATL ACAD SCI USA, V101, P2864, DOI 10.1073-pnas.0400164101; Jakab K, 2008, TISSUE ENG PT A, V14, P413, DOI 10.1089-tea.2007.0173; Jay SM, 2008, FASEB J, V22, P2949, DOI 10.1096-fj.08-108803; Jiang B, 2013, TISSUE ENG PT A, V19, P224, DOI 10.1089-ten.tea.2012.0120; Kaigler D, 2006, J BONE MINER RES, V21, P735, DOI 10.1359-JBMR.060120; Kaihara S, 2000, TISSUE ENG, V6, P105, DOI 10.1089-107632700320739; Kakisis JD, 2005, J VASC SURG, V41, P349, DOI 10.1016-j.jvs.2004.12.026; Katanosaka Y, 2008, J BIOTECHNOL, V133, P82, DOI 10.1016-j.jbiotec.2007.09.017; Kaully T, 2009, TISSUE ENG PART B-RE, V15, P159, DOI 10.1089-ten.teb.2008.0193; Khademhosseini A, 2006, P NATL ACAD SCI USA, V103, P2480, DOI 10.1073-pnas.0507681102; Khademhosseini A, 2007, BIOMATERIALS, V28, P5087, DOI 10.1016-j.biomaterials.2007.07.021; Kim S, 2013, LAB CHIP, V13, P1489, DOI 10.1039-c3lc41320a; King KR, 2004, ADV MATER, V16, P2007, DOI 10.1002-adma.200306522; Konig G, 2009, BIOMATERIALS, V30, P1542, DOI 10.1016-j.biomaterials.2008.11.011; Koroleva A, 2012, BIOFABRICATION, V4, DOI 10.1088-1758-5082-4-1-015001; Kothapalli CR, 2011, LAB CHIP, V11, P497, DOI [10.1039-c0lc00240b, 10.1039-c01c00240b]; Ku DN, 1997, ANNU REV FLUID MECH, V29, P399, DOI 10.1146-annurev.fluid.29.1.399; Kusuma S, 2013, P NATL ACAD SCI USA, V110, P12601, DOI 10.1073-pnas.1306562110; Lafleur Marc A, 2003, Expert Rev Mol Med, V5, P1; Lee KY, 2001, CHEM REV, V101, P1869, DOI 10.1021-cr000108x; Lee SH, 2008, BIOMATERIALS, V29, P2962, DOI 10.1016-j.biomaterials.2008.04.004; Leslie-Barbick JE, 2011, BIOMATERIALS, V32, P5782, DOI 10.1016-j.biomaterials.2011.04.060; Levenberg S, 2005, NAT BIOTECHNOL, V23, P879, DOI 10.1038-nbt1109; L'Heureux N, 2006, NAT MED, V12, P361, DOI 10.1038-nm1364; Lillie MA, 2007, BIOMATERIALS, V28, P2021, DOI 10.1016-j.biomaterials.2007.01.016; Ling Y, 2007, LAB CHIP, V7, P756, DOI 10.1039-b615486g; Liu Y, 2012, ACS APPL MATER INTER, V4, P1378; McGrath JC, 2005, EXP PHYSIOL, V90, P469, DOI 10.1113-expphysiol.2005.030130; McGuigan AP, 2007, BIOMATERIALS, V28, P2547, DOI 10.1016-j.biomaterials.2007.01.039; Miller JS, 2012, NAT MATER, V11, P768, DOI [10.1038-nmat3357, 10.1038-NMAT3357]; Mitchell SL, 2003, CARDIOVASC PATHOL, V12, P59, DOI 10.1016-S1054-8807(02)00183-7; Monos E, 2001, AM J PHYSIOL-HEART C, V281, pH1606; Moon JJ, 2009, TISSUE ENG PT A, V15, P579, DOI 10.1089-ten.tea.2008.0196; Moon JJ, 2010, BIOMATERIALS, V31, P3840, DOI 10.1016-j.biomaterials.2010.01.104; Moosmang S, 2003, EMBO J, V22, P6027, DOI 10.1093-emboj-cdg583; Mosadegh B, 2007, LANGMUIR, V23, P10910, DOI 10.1021-la7026835; Naderi H, 2011, J BIOMATER APPL, V26, P383, DOI 10.1177-0885328211408946; Nichol JW, 2010, BIOMATERIALS, V31, P5536, DOI 10.1016-j.biomaterials.2010.03.064; Nikkhah M, 2012, BIOMATERIALS, V33, P9009, DOI 10.1016-j.biomaterials.2012.08.068; Nikkhah M, 2012, BIOMATERIALS, V33, P5230, DOI 10.1016-j.biomaterials.2012.03.079; Nillesen STM, 2007, BIOMATERIALS, V28, P1123, DOI 10.1016-j.biomaterials.2006.10.029; Norotte C, 2009, BIOMATERIALS, V30, P5910, DOI 10.1016-j.biomaterials.2009.06.034; Oberleithner H, 2007, P NATL ACAD SCI USA, V104, P16281, DOI 10.1073-pnas.0707791104; Owens GK, 2004, PHYSIOL REV, V84, P767, DOI 10.1152-physrev.00041.2003; Park JH, 2009, BIOTECHNOL BIOENG, V106, P138; Patan S, 2000, J NEURO-ONCOL, V50, P1, DOI 10.1023-A:1006493130855; Paul A, 2014, ADV DRUG DE IN PRESS; Perets A, 2003, J BIOMED MATER RES A, V65A, P489, DOI 10.1002-jbm.a.10542; Phillippi JA, 2008, STEM CELLS, V26, P127, DOI 10.1634-stemcells.2007-0520; Polverini PJ, 2012, ENDOD TOP, V24, P130; Price GM, 2010, BIOMATERIALS, V31, P6182, DOI 10.1016-j.biomaterials.2010.04.041; Price GM, 2008, J AM CHEM SOC, V130, P6664, DOI 10.1021-ja711340d; Price GM, 2008, MICROVASC RES, V76, P46, DOI 10.1016-j.mvr.2008.02.003; Raghavan S, 2010, TISSUE ENG PT A, V16, P2255, DOI 10.1089-ten.TEA.2009.0584; Ratcliffe A, 2000, MATRIX BIOL, V19, P353, DOI 10.1016-S0945-053X(00)00080-9; Rensen SSM, 2007, NETH HEART J, V15, P100; Rhodin JAG, 1980, HDB PHYSL 2, P1; Ruiz SA, 2007, SOFT MATTER, V3, P168, DOI 10.1039-b613349e; Sadr N, 2011, BIOMATERIALS, V32, P7479, DOI 10.1016-j.biomaterials.2011.06.034; Sarkar S, 2006, BIOMATERIALS, V27, P4775, DOI 10.1016-j.biomaterials.2006.04.038; Sartore S, 2001, CIRC RES, V89, P1111, DOI 10.1161-hh2401.100844; Saunders RE, 2008, BIOMATERIALS, V29, P193, DOI 10.1016-j.biomatei-ials.2007.09.032; Schechter AN, 2003, NEW ENGL J MED, V348, P1483, DOI 10.1056-NEJMcibr023045; SCHWARTZ SM, 1986, CIRC RES, V58, P427; Seifalian AM, 2002, ARTIF ORGANS, V26, P307, DOI 10.1046-j.1525-1594.2002.06841.x; Seliktar D, 2000, ANN BIOMED ENG, V28, P351, DOI 10.1114-1.275; Shadwick RE, 1999, J EXP BIOL, V202, P3305; Shamloo A, 2010, LAB CHIP, V10, P3061, DOI 10.1039-c005069e; Shin M, 2004, BIOMED MICRODEVICES, V6, P269, DOI 10.1023-B:BMMD.0000048559.29932.27; Sieminski AL, 2004, EXP CELL RES, V297, P574, DOI 10.1016-j.yexcr.2004.03.035; Silva EA, 2010, BIOMATERIALS, V31, P1235, DOI 10.1016-j.biomaterials.2009.10.052; Singh S, 2011, BIOMATERIALS, V32, P2059, DOI 10.1016-j.biomaterials.2010.11.038; Smith CM, 2004, TISSUE ENG, V10, P1566, DOI 10.1089-1076327042500274; Smith CM, 2007, TISSUE ENG, V13, P373, DOI 10.1089-ten.2006.0101; Song JW, 2012, INTEGR BIOL-UK, V4, P857, DOI 10.1039-c2ib20061a; Stegemann JP, 2007, TISSUE ENG, V13, P2601, DOI 10.1089-ten.2007.0196; Stroncek JD, 2011, TISSUE ENG PT A, V17, P2091, DOI [10.1089-ten.TEA.2010.0631, 10.1089-ten.tea.2010.0631]; Sudo R, 2009, FASEB J, V23, P2155, DOI 10.1096-fj.08-122820; Takei T, 2006, BIOTECHNOL BIOENG, V95, P1, DOI 10.1002-bit.20903; Tamayol A, 2013, BIOTECHNOL ADV, V31, P669, DOI 10.1016-j.biotechadv.2012.11.007; Taylor RN, 2009, REPROD SCI, V16, P140, DOI 10.1177-1933719108324893; Therriault D, 2003, NAT MATER, V2, P265, DOI 10.1038-nmat863; Toohey KS, 2009, ADV FUNCT MATER, V19, P1399, DOI 10.1002-adfm.200801824; Tremblay PL, 2005, AM J TRANSPLANT, V5, P1002, DOI 10.1111-j.1600-6143.2005.00790.x; Tsai M, 2012, J CLIN INVEST, V122, P408, DOI 10.1172-JCI58753; Tsuda V, 2007, BIOMATERIALS, V28, P4939; Turturro MV, 2013, PLOS ONE, V8, DOI 10.1371-journal.pone.0058897; Unger MA, 2000, SCIENCE, V288, P113, DOI 10.1126-science.288.5463.113; Vickerman V, 2008, LAB CHIP, V8, P1468, DOI 10.1039-b802395f; Weinstein BM, 1999, DEV DYNAM, V215, P2, DOI 10.1002-(SICI)1097-0177(199905)215:12::AID-DVDY23.0.CO;2-U; Williamson A, 2013, LAB CHIP, V13, P3471, DOI 10.1039-c3lc50237f; Wong KHK, 2012, ANNU REV BIOMED ENG, V14, P205, DOI [10.1146-annurev-bioeng-071811-150052, 10.1146-annurev.bioeng-071811-150052]; Wu W, 2011, ADV MATER, V23, pH178, DOI 10.1002-adma.201004625; Xu T, 2005, BIOMATERIALS, V26, P93, DOI 10.1016-j.biomaterials.2004.04.011; Xu T, 2006, BIOMATERIALS, V27, P3580, DOI 10.1016-j.biomaterials.2006.01.048; Yancopoulos GD, 2000, NATURE, V407, P242, DOI 10.1038-35025215; Yeon JH, 2012, LAB CHIP, V12, P2815, DOI 10.1039-c2lc40131b; Yoshida H, 2012, ADV FUNCT MATER, V23, P1736; Zhang WJ, 2007, J CELL MOL MED, V11, P945, DOI 10.1111-j.1582-4934.2007.00099.x; Zheng Y, 2012, P NATL ACAD SCI USA, V109, P9342, DOI 10.1073-pnas.1201240109; Zisch AH, 2001, J CONTROL RELEASE, V72, P101, DOI 10.1016-S0168-3659(01)00266-83
Molecular Architecture of the Mouse Nervous System
No full textThe mammalian nervous system executes complex behaviors controlled by specialized, precisely positioned, and interacting cell types. Here, we used RNA sequencing of half a million single cells to create a detailed census of cell types in the mouse nervous system. We mapped cell types spatially and derived a hierarchical, data-driven taxonomy. Neurons were the most diverse and were grouped by developmental anatomical units and by the expression of neurotransmitters and neuropeptides. Neuronal diversity was driven by genes encoding cell identity, synaptic connectivity, neurotransmission, and membrane conductance. We discovered seven distinct, regionally restricted astrocyte types that obeyed developmental boundaries and correlated with the spatial distribution of key glutamate and glycine neurotransmitters. In contrast, oligodendrocytes showed a loss of regional identity followed by a secondary diversification. The resource presented here lays a solid foundation for understanding the molecular architecture of the mammalian nervous system and enables genetic manipulation of specific cell types. Single-cell transcriptional profiling of the adult mouse nervous system uncovers new cell classes and types across regions, providing a clearer picture of cell diversity by region and a reference atlas for studying the mammalian nervous system. © 2018 The Author
