82 research outputs found
Analysis of flowering gene in palm oil (Elaeis guineensis)
No full textFaizal I, Emdi A. 2017. Analysis of flowering gene in palm oil (Elaeis guineensis). Asian J Agric 1: 53-58. Palm oil has always been an important commodity in Indonesia. The most common species is palm oil, Elaeis guineensis. Palm oil is a monoecious plant with a tendency to be a temporal dioecious. Female flower will be the one that produces palm oil fruit, that later is treated with palm oil while male flower only takes part in the fertilization process. In order to know the ratio between female and male flower tree in a plantation, this study was performed to detect a distinction between female and male flowering gene sequences from DNA sample of E. guineensis. Based on previous study which managed to characterize MADS-box gene of palm oil, a primer was designed and named GmG (Globosa-male-Gaps). The result shows that the primer has the ability to differentiate DNA sequence female and male flower of E.guineensis, Palm oil. However, further studies with full sequence and more samples are needed to find distinctive results between female and male flower sequences as the GmG primer could be used to design a specific marker or primer to detect the presence of female or male flower within a tree.</jats:p
Bioproduction of Cresol from Toluene in a Two-Liquid-Phase Culture System by a Solvent-Tolerant Bacteria
No full textA toluene-metabolizing bacterium, designated Pseudomonas putida T-57, was isolated from soil samples. The strain was able to grow on toluene when liquid toluene was added to mineral salts basal medium at 10&ndas
UPAYA PENCEGAHAN KETERLAMBATAN KM. DOBONSOLO MELALUI OPTIMALISASI KEGIATAN EMBARKASI DAN DEBARKASI PENUMPANG
No full textABSTRAKSI
Irvan Fadillah Nurwantoro NIT: 51145220 N, 2019, "Upaya Pencegahan
Keterlambatan KM. DOBONSOLO Melalui Optimalisasi Kegiatan
Embarkasi dan Debarkasi Penumpang", Skripsi Program Studi Nautika,
Program Diploma IV, Politeknik Ilmu Pelayaran Semarang, Pembimbing I:
Capt. Ali Imran Ritonga, MM, M.Mar. dan Pembimbing II: H. Mustholiq,
MM.
Pelaksanaan embarkasi dan debarkasi yang cepat dan aman serta lancar
merupakan tujuan utama para penumpang. Upaya tersebut di maksudkan agar kapal
dan muatan serta keselamatan para penumpang bisa terjamin saat proses embarkasi
dan debarkasi. Proses embarkasi dan debarkasi yang terjadi di kapal KM.
DOBONSOLO sewaktu di pelabuhan Tanjung Perak Surabaya adalah kurang
efisiennya manajemen untuk mengatur penumpang yang akan naik dan turun, serta
kurangnya fasilitas yang memadai di terminal pelabuhan.Sehingga para penumpang
saling berdesakkan dan dapat mengakibatkan atau membahayakan jiwa para
penumpang tersebut. Sedangkan PT. PELNI sebagai penyedia sarana transportasi
pun tidak meningkatkan pelayanan–pelayanan diatas kapal, bahkan terkesan hanya
mencari keuntungan tanpa memperdulikan kualitas pelayanan.
Berdasarkan hasil penelitian yang telah penulis lakukan, menunjukkan
beberapa faktor-faktor yang menghambat jalannya proses embarkasi dan debarkasi
penumpang. Faktor-faktor tersebut adalah maraknya pedagang asongan dan tidak
tertibnya buruh-buruh bagasi dalam pengangkutan barang milik
penumpang,merebaknya pengantar dan pengunjung yang tidak mengindahkan
peraturan serta pengumuman yang telah dilakukan oleh pihak kapal sehingga
terkadang mereka terbawa dalam pelayaran yang sering disebut Penumpang Tanpa
Tiket (PTT), faktor yang lain adalah keterlambatan kapal sewaktu tiba di pelabuhan
tujuan, faktor terakhir adalah barang bawaan penumpang yang berlebih (Over
Baggage).
Adapun upaya yang dilakukan oleh pihak kapal dan petugas darat adalah
dengan meningkatkan kerja sama yang baik antara petugas darat dan petugas kapal
sehingga dapat mendukung kelancaran proses embarkasi dan debarkasi.
ABSTRACT
Irvan Fadillah Nurwantoro NIT: 51145220 N, 2019, "Upaya Pencegahan
Keterlambatan KM. DOBONSOLO Melalui Optimalisasi Kegiatan
Embarkasi dan Debarkasi Penumpang", Nautical Study Program,
Diploma IV Program, Merchant Marine Polytechnic of Semarang, 1st
Advisor: Capt. Ali Imran Ritonga, MM, M.Mar. and 2nd Advisor: H.
Mustholiq, MM.
The fast and safe and smooth implementation of embarkation and
debarkation is the main goal of the passengers. The effort is intended so that the
ship and cargo and the safety of passengers can be guaranteed during the
embarkation and debarkation process. The process of embarkation and debarkation
that occurs on KM ships. DOBONSOLO while at the port of Tanjung Perak
Surabaya was management inefficient to regulate passengers who were going up
and down, as well as the lack of adequate facilities at the port terminal. So that
passengers crowded together and could cause or endanger the lives of these
passengers. Whereas PT. PELNI as a means of transportation also does not improve
services on board, even seems to only seek profits regardless of service quality.
Based on the results of the research that the author has done, it shows several
factors that hinder the process of embarkation and debarkation of passengers. These
factors are the rise of hawkers and the disorderly practice of baggage workers in
transporting passenger property, widespread outreach and visitors who do not heed
the rules and announcements made by the ship so that sometimes they are carried
on a cruise which is often called a Passenger Without Tickets ( PTT), another factor
is the delay of the ship when it arrives at the destination port, the last factor is excess
baggage (Over Baggage).The efforts made by the ship and land officers are to increase good
cooperation between land officials and ship officers so that they can support the
smooth process of embarkation and debarkation
FIG. 5 in A New Genus and Two New Species of Arboreal Toads from the Highlands of Sumatra with a Phylogeny of Sundaland Toad Genera
No full textFIG. 5.—Habitat of Sigalegalephrŋnus mandailinguensis—a view of the rainforest at the edge of an inactive solphatara field on the northeastern slope of Gunung Sorikmarapi where the holotype was found (upper); and first author at the entrance of the subterranean hollow where the holotype was collected (lower).Published as part of Smart, Utpal, Sarker, Goutam C., Arifin, Umilaela, Harvey, Michael B., Sidik, Irvan, Hamidy, Amir, Kurniawan, Nia & Smith, Eric N., 2017, Herpetologica 73 (1) on pages 63-75, DOI: 10.1655/Herpetologica-D-16-00041, http://zenodo.org/record/771643
Analisis daya pengereman pada rem cakram roda depan kendaraan motor listrik e-niaga roda 3 geni biru
No full textBrakes are systems used to control, slow down and stop wheel rotation. Failures in the braking system have a very fatal impact on the driver and other drivers. Because it is very important from the function of the vehicle brake system, it is necessary to conduct in-depth research on the braking power needed so that the brake system can work perfectly and stop according to the standard braking distance. The purpose of this study is to be able to determine the amount of braking power required for the disc brake type on the blue Geni 3-wheeled E-Commerce electric motor to be able to stop the vehicle perfectly. The test method that will be used is to test the braking road with a vehicle variation speed of 20 Km / h, 30 Km / h and 40 Km / h with a maximum vehicle stop distance of 25 meters. From the results of theoretical calculations and tests on vehicleS E- obtained the results of the braking power required at a speed of 20 Km / h of 3786.97 J / s with a vehicle stop distance of 7.4 meters and a braking force of 1371 N, at a speed of 30 Km / h braking power of 7563.79 J / s with a stopping distance of 12.26 meters and a braking force of 1820 N, at a speed of 40 Km / h The braking power is 11118.25 J/s with a vehicle stop distance of 19.8 meters and a braking force of 2021.5
Identification of MADS-box Gene in Oil Palm (Elaeis guineensis Jacq.)
No full textThe bunch size represented by the fruit number is the main parameter of oil palm (Elaeis guineensis Jacq.) yield. The fruit number, which is determined during the initial phase of development, is related to various factors, including the genetic properties of the trees. Trees that have more pistillate flowers have more fruit. The diversity of MADS-box genes assumed can be used as a marker for trees that have a higher number of pistillate flowers. Therefore, the aims of this research were to isolate and identify the MADS-box genes from flowers of tenera oil palm using PCR techniques. The SQUAMOSA (SQUA) gene and the GLOBOSA (GLO) gene are members of the MADS-box genes family that are responsible for sepal, petal and stamen organ development. The genomic DNA of the staminate flowers of trees that have more staminate flowers (P1) and the genomic DNA of the pistillate flowers of trees that have more pistillate flowers (P2) were isolated using the CTAB+ PVP method. The CTAB+PVP method was more efficient for isolating pistillate flower genomic DNA than staminate flower genomic DNA. The genomic DNA of P1 and P2 was amplified with two primers: BMS and BMG. The BMS primers gave a PCR product size of 1250 bp for the genomic DNA of P1 and P2. Meanwhile, the BMG primers gave a PCR product size of 1250 bp and 1300 bp for P1 and P2, respectively. The PCR products were sequenced and analyzed for homology using the GenBank database. BLAST analysis showed the PCR products have high homology with the SQUA1 gene and the GLO2 gene. Alignment analysis showed that the DNA fragments amplified with the BMS primers of the P1 and P2 sequences have variations in the exons and introns, and the variations were observed only in the introns of the DNA fragments amplified with the BMG primers
Kloning gen virulen Streptococcus agalactiae sebagai bahan dasar vaksin rekombinan
No full textABSTRACT
Streptococcus agalactiae has emerged as an important pathogen that affects Nile tilapia in Indonesia aquaculture. Vaccination is one of the most effective tools for enhancing host defense and protecting fish from pathogens. DNA vaccine is a third generation of vaccines based on the gene encoding a vaccine antigen rather than the antigen itself. Mga is DNA-binding protein that activates expression of several important virulence gene, including those encoding M protein (emm), C5a peptidase (SCPA) and mga. The goals of this study were to isolate and molecular characterize the mga gene of local isolate of S. agalactiae to support the development of DNA vaccine. Local bacterial strain was isolated from Nile tilapia farming in West Java, Indonesia. Bacterial identification was accomplished by PCR, using 16S rRNA primers, which revealed the 1,500 bp PCR product. Mga gene isolation was accomplished by PCR using mga gene S. agalactiae SAF and SAR- specific primers, which revealed the 1,494 bp PCR product. Mga gene was cloned into pGEM T-easy and sequenced using M13 primers. SalI and NotI restriction enzymes were used to digest the pGEM T-easy vector containing mga gene. Mga gene was cloned into pMBA containing beta actin promoter of Japanese medaka. The 16S rRNA sequence analyses confirmed that the local bacteria was 97% similarity with S. agalactiae strain 15-92MPnew. The nucleotide sequence analyses confirmed that the clones were contained 98% similarity with M protein mga S. agalactiae. The mga gene controlled by MBA promoter has constructed successfully as a candidate of DNA vaccine to against S. agalactiae infection in Nile tilapia.
Keywords: DNA Vaccine, Streptococcus agalactiae, mga gene, Oreochromis niloticus, recombinant DNA
ABSTRAK
Streptococcus agalactiae merupakan patogen penting yang mempengaruhi budidaya ikan nila di Indonesia. Vaksinasi merupakan salah satu metode yang paling efektif untuk meningkatkan pertahanan dan melindungi ikan dari patogen. Vaksin DNA adalah vaksin generasi ketiga yang mengandung gen penyandi antigen vaksin. Mga adalah protein DNA-binding yang mengaktifkan ekspresi beberapa gen virulensi, termasuk M protein (emm), C5a peptidase (SCPA) dan mga. Tujuan dari penelitian ini adalah untuk mengisolasi dan karakterisasi secara molekuler gen mga dari isolat lokal S. agalactiae untuk mendukung pengembangan vaksin DNA. Identifikasi bakteri dilakukan dengan PCR, menggunakan primer 16S rRNA dengan produk PCR 1.500 bp. Isolasi gen mga dilakukan dengan metode PCR menggunakan primer SAF dan SAR dengan ukuran target 1.494 bp. Gen mga dikloning ke vektor pGEMT–easy dan disekuensing menggunakan primer M13. Enzim Sal I dan Not I digunakan untuk memotong gen mga dari vektor pGEMT- easy, selanjutnya gen mga dikloning ke vektor pMBA yang mengandung promoter beta-aktin ikan medaka Jepang. Berdasarkan analisis menggunakan gen 16S rRNA diperoleh bahwa sampel memiliki kesamaan 97% sebagai S. agalactiae. Analisis sekuen nukleotida menunjukkan bahwa klon mengandung gen mga dengan 98% kesamaan dengan M protein mga S. agalactiae. Konstruksi mga gene yang dikendalikan oleh promoter MBA telah berhasil dibuat dan ini merupakan kandidat vaksin DNA untuk mengendalikan infeksi S. agalactiae pada ikan nila.
Kata kunci: Vaksin DNA, Streptococcus agalactiae, gen mga, Oreochromis niloticus, DNA rekombinan
Development and Optimization of SARS-CoV-2-Specific Primers for Accurate Diagnosis: A Case Study in West Sumatra - Indonesia
No full textBackground: In 2022, new cases of Covid-19 emerged, including the Omicron variant which is classified as a variant of concern (VOC). West Sumatra is one of the top ten provinces with the highest number of cases in Indonesia. This study aimed to design specific primers and optimize the PCR method that can be used for accurate detection, specifically for SARS-CoV-2 circulating in West Sumatra.Methods: This study used an in silico approach, using whole genome sequencing (WGS) data available at the global initiative on sharing avian influenza data (GISAID), and employing the Geneious Prime application which confirmed samples collected from Padang, West Sumatra, and from Jakarta, Bogor, Depok, Tangerang, Bekasi (Jabodetabek) serving as comparative sample tests. Technology development was supported by bioinformatics testing, laboratory testing, and validation methods, involving gene mining, sequence alignment, and primer design. Laboratory tests and validation included viral genomes extraction and cDNA synthesis, polymerase chain reaction (PCR) testing, and results analysis. Results: Three sets of optimal primer candidates amplified the coveted target gene was discovered, specifically, the S gene of the receptor binding domain (RBD) region.Conclusions: The primers designed through a consensus between the complete genome of the SARS-CoV-2 isolate Wuhan-Hu-1 and the WGS of the Omicron variant in Padang, West Sumatra, have successfully detected the SARS-CoV-2 virus variant in the region. The most effective temperature optimization results were achieved by testing three primer products on samples from Padang and Jabodetabek. It has significance as a valuable diagnostic tool in the primer form
Diversifikasi Pendapatan, Risiko Kredit, Loan to Deposit Ratio, Risk Aversion Dan Net Interest Margin
No full textThis research aims to analyze the influence of income diversification, credit risk, loan to deposit ratio, and risk aversion to Net Interest Margin (NIM) of conventional bank listed in IDX during the periods 2014 - 2016. Income diversification is measured by NII ratio, credit risk is measured by NPL ratio, loan to deposit ratio is measured by LDR, and risk aversion is measured by CAR. This research also using bank size and BOPO as control variable.
The data studied were obtained through non-participant observation method by directly quoting financial and banking data. The data sources used in this study came from Bloomberg and OJK. The sampling technique used was purposive sampling. Based on the criteria determined, it will get 36 conventional banks. Data analysis in this study used multiple linear regression analysis, which had previously passed the classical assumption test.
Result of this research show that income diversification, credit risk, loan to deposit ratio, and risk aversion have positive and significance effect to net interest margin
Effect of agitation on methanogenesis stage of two-stage anaerobic digestion of palm oil mill effluent (POME) into biogas
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