1,721,100 research outputs found
Biologia, diagnosi e controllo degli acari
No full textl'articolo rientra in un supplemento de ""la Settimana Veterinaria" dedicato a "controllo degli ectoparassiti: le linee guida Esccap. Si tratta di linee guida per il controllo dei parassiti prodotte dall'European Scientific Counsel companion animal parasites (Esccap) tradotte e adattate alla realtà italiana, che riassumono quanto emerge dagli studi su epidemiologia, terapia e controllo delle parassitosi in Europa
Lo Quarter: il cimitero degli appestati di Alghero (1582-1583 AD)
No full textI resti scheletrici di 400 presunti appestati deceduti durante l'epidemia del 1582-1583 sono stati esumati dal cortile dell'ex Collegio Gesuitico del quartiere San Michele - Lo Quarter (Alghero, Sardegna). Sono state identificate sedici trincee ciascuna contenente i resti di 10-30 individui deceduti in un breve lasso di tempo e inumati simultaneamente.
I campioni ossei di quattro individui esumati da altrettante trincee sono stati analizzati, tramite tecnica immunocromatografica (RDT peste), al fine di verificare se l’infezione da Yersinia pestis fosse stata la causa del loro decesso. L’antigene F1 di Y. Pestis è stato identificato nel 100 % dei campioni testati con concentrazioni comprese tra 2.5 ng/ml e 0.625 ng/ml.
Si conferma che i succitati individui erano affetti dall’infezione pestosa al momento del loro decesso. Attualmente, è in corso uno studio antropologico, paleo patologico e paleo immunologico sui resti scheletrici di 6 individui esumati dalla trincea 9.
Per le sue caratteristiche e dimensioni, questo cimitero della peste rappresenta un unicum in Italia ed è comparabile solo al cimitero degli appestati di Martigues (Francia), di epoca ben più tarda (1720-1721 AD)
Isolation of Brucella melitensis from Alpine ibex
No full textEleven alpine ibex (Capra ibex) and 27 chamois (Rupicapra rupicapra) from Gran Paradiso National Park (Italy) were examined in March 1996. A 7-yr-old ibex buck had thick-walled carpal joints and enlargement of the right testicle characterized by necrosis and fibrosis. Microscopically, testicular lesions were characterized by large areas of necrosis, fibrosis with irregular aggregates of macrophages and lymphocytes, and scattered foci of suppuration. Specimens of the carpal bursae and testicle were cultured in serum dextrose agar and serum dextrose antibiotic plates. A Gram-negative coccobacillus was isolated from the testicle and subsequently identified as Brucella melitensis biotype 2. This is the first recognized case of brucellosis in alpine ibex
Plasmodium falciparum immunodetection in bone remains of members of the Renaissance Medici family (Florence, Italy, sixteenth century)
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“Immunohistochemical analysis of the leishmanin reaction in dogs from an endemic area for Leishmania infantum.”
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Malaria was the killer of Francesco I de' Medici (1531-1587)
No full textThe sudden deaths of Francesco I de’ Medici (1531-1587), Second Grand Duke of Tuscany (Figure 1a), and his wife, Bianca Cappello (1548-1587), have been shrouded in mystery, and the cause of death has been debated for the past 4 centuries. Cancellous bone was harvested from a vertebra of Francesco I. Bone samples of Cosimo I de’ Medici, who died of pneumonia, and his daughter-in-law, Joan of Austria, who died in childbirth, were used as negative controls. In addition, 2 medieval bone samples from 2 sites known to be free from malaria (Briançon, France, 17th century; Augsburg, Germany, 14th century) also were used as negative controls. Extracts prepared from spongy bone samples were examined for the presence of P. falciparum histidine-rich protein 2 and P. falciparum lactate dehydrogenase using 2 commercial qualitative double-antibody immunoassays: Malaria Antigen RAPYDTEST and Malaria Detect RAPYDTEST (DiaSys, Waterbury, Conn). Positive results were obtained from both dipstick assays. We therefore provide the first biological evidence of the presence of both P. falciparum ancient proteins (P. falciparum histidine-rich protein 2 and P. falciparum lactate dehydrogenase) in the skeletal remains of Francesco I de’ Medici. No mixed falciparum infections or non-falciparum infections were identified. Bone samples from Cosimo I, Joan of Austria, and control samples were all negative, as expected. Our recent findings support the detailed medical documents recorded by court physicians who carefully described the different stages of the sudden illness that affected the Grand Duke Francesco I until his demise. Muscle has, thus far, been considered the best tissue for the detection of P. falciparum malaria because of its abundant red cell content.6 We now show that malaria antigens also can be detected in ancient bone samples. With the use of modern methods, we provide robust evidence that Francesco I had falciparum malaria at the time of his death. Our immunologic results confirm the archival sources that described the onset, course, and fatal outcome of the disease. Our findings also absolve Ferdinando I from the shameful allegation of being the murderer of his brother and sister-in-law
Capitolo 3: linee guida per il controllo degli ectoparassiti del cane e del gatto in :
No full textPoiché molti ectoparassiti sono vettori di numerose e importanti malattie degli animali d’affezione, lo scopo di ESCCAP è di realizzare e diffondere informazioni e un supporto completo per assistere sia i medici veterinari sia i proprietari di animali, al fine di controllare in modo efficace le infestazioni da ectoparassiti e prevenire, nel contempo, la trasmissione delle malattie veicolate dagli stessi. Queste linee guida sono focalizzate sui gruppi più importanti di ectoparassiti come pulci, zecche e pidocch
Molecular assay for the identification of Setaria tundra
No full textWe report the molecular characterization of the amplification products obtained by specific PCR experiments aimed to identify the 5S ribosomal spacer of Setaria tundra specimens isolated from roe deer (Capreolus capreolus) in north Italy, which represent the first record of the parasite in this country. Three different fragments of approximately 400, 800, and 1200 bp (base pairs) are produced. Sequence analyses showed that all three fragments share a very high level of similarity to 5S spacer sequences of some Setaria species and other filariae present in genebank. Based on these sequences, we were able to design species-specific PCR primers for the precise identification of S. tundra
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