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    Effect of dietary phenylpropanoid glycoside-based natural extracts on blood parameters and productive performance in intensively-reared young hares

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    Two different doses of a dietary verbascoside based supplement were evaluated on some various blood parameters and on productive performance in young weaned hares (28d old), reared intensively from 28 to 90 days. The study lasted 62 days and was conducted on 210 young hares divided into three homogenous groups of 70 animals each, consisting of a control group and two experimental groups. Each group received the dietary supplement in the feed, titrated to 0.5% verbascoside, 1 kg/t of concentrate (LVB group) and 2 kg/t of concentrate (HVB group). The experimental reliefs included the measurement of blood parameters such as triglycerides, total cholesterol, high density lipoprotein cholesterol, and bilirubin, along with some markers of oxidative status in plasma such as reactive oxygen metabolites, thiobarbituric acid-reactive substances, and vitamins A and E. Various productive parameters were also measured such as body weight and growth rate, food consumption and feed conversion. The experimental treatment resulted in a significant decrease in triglycerides (P<0.01) and bilirubin (P<0.05) and an increase in high density lipoprotein cholesterol (P<0.01 ) in the LVB and HVB experimental groups. In addition, the oxidative plasma stability in the blood also improved, with a significant decrease in the concentration of reactive oxygen metabolites (P<0.01) and thiobarbituric acid-reactive substances (P<0.01), together with increased levels of vitamin E (P<0.05). The productive performance was not statistically influenced by the experimental treatment, except for the growth rate which increased significantly (P<0.05) in the experimental LVB and HVB groups: 6.9% and 8.7% respectively compared with the control group

    Effect of dietary extruded linseed, verbascoside and vitamin E supplements on selected serum biochemical parameters and plasma oxidative status in Lacaune ewes

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    Selected serum biochemical parameters and plasma oxidative status were evaluated in Lacaune ewes on a diet supplemented with extruded linseed, verbascoside and vitamin E. A 98 day-trial was conducted on 44 ewes and started 40 ± 2 days post partum. The animals were divided into four homogeneous groups of eleven animals each; one control group (CON) with a basal diet, and the other three experimental groups supplemented with extruded linseed (L group), extruded linseedverbascoside (LVB group) and extruded linseed-verbascoside-vitamin E (LVBE group). All animals individually received an isoproteic and isoenergetic diet, consisting of 700g of concentrated feed and meadow hay ad libitum. Blood sampling of the ewes was performed three times: at the beginning (0 d), midway (49 d) and end of the trial (98 d). The following parameters were determined: triglycerides, total cholesterol, high-density lipoprotein (HDL) cholesterol, low-density lipoprotein (LDL) cholesterol, bilirubin, alanine aminotransferase, aspartate aminotransferase, thiobarbituric acid reactive substances (TBARS), reactive oxygen metabolites (ROMs), vitamin A and vitamin E. Dietary integration in the LVB and LVBE groups resulted in an improvement in the blood profile, characterized by a significant (P=0.001) increase in HDL cholesterol and a marked decrease in triglycerides (P=0.001), total cholesterol (P=0.017), LDL cholesterol (P=0.001) and bilirubin (P=0.035), due to the verbascoside supplementation. In the same groups, there was an increase in blood vitamin A (P=0.002) and vitamin E (P=0.001), and a reduction (P=0.001) in ROMs and TBARS, with an improved plasma oxidative status. The dietary vitamin E integration did not produce a significant improvement in the parameters studied, with no statistical differences between LVB and LVBE groups. Animal feed containing extruded flaxseed, might therefore benefit from the addition of a verbascoside supplement, because of the positive effect showed by this molecule on the blood parameters

    Reproductive indices in rabbit does after dietary supplementation with nutraceutical substances: Preliminary data

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    Aim of the present work was to investigate the effect of Chlorella vulgaris and Laurus nobilis on selected reproductive indices in intensive reared rabbit does. Second parity New Zealand White does (n = 60) were enrolled for two consecutive reproductive cycles. Rabbit does were randomly assigned to one of four experimental groups (n = 15 replicates per treatment). The first control group (CON) received a commercial pellet-diet; experimental CHL group received the same diet supplemented with 0.1% of dried Chlorella algae powder; experimental LAU group received control diet supplemented with 0.1% of dried bay leaves powder; experimental MIX group received control diet supplemented with a mixture of the same doses of both natural dried plants. Considering data on the first reprodcutive cycle, dietary treatments did not influence (P&gt;0.05) the number of kits per litter (born alive and dead) and number of animals at the weaning period. A significant effect (P&lt;0.05) was observed on kits mortality rate (percentage of mortality during lactation), with LAU group as the lowest value among all experimental groups. No effect was notice on parameters regarding weight of litter, weight of single kit and average daily gain during the experimental period. In conclusion, no detrimental effect was observed on reproductive parameters after the use of Chlorella algae and bay leaves as feed supplements alone and in combination. Among them, these preliminary data underline a lower mortality of kits during lactation period, obtained in rabbit does fed with Laurus nobilis

    Effects of verbascoside on plasma oxidative status and blood and milk production parameters during the peripartum period in Lacaune ewes

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    This study examined the effect of administration of verbascoside on plasma oxidative status and on specific blood and milk production parameters in Lacaune ewes during the peripartum period. The trial, lasting 60 d, ran from 20 d before lambing until 40 d after and was conducted on 40 ewes in their 5th month of gestation. The animals were divided into two equal groups of 20: a control group CON, which did not receive food supplement, and the experimental group V, which received a supplement of verbascoside in their feed. Consumption of the supplement, which was 2 g/ewe/d, was assured by individual and daily administration of feed. The animals received a pellet feed concentrate at a rate of 400 g/ewe/d prepartum and 700 g/ewe/d postpartum. The ration was completed with mixed hay ad libitum. The experimental measures included body weight; blood samples 20 d before lambing and 0 d, 20 d and 40 d after lambing; body condition score; individual yield and quality of milk. The administration of verbascoside significantly improved milk yield by 9.3% (P < 0.01), significantly decreased triglycerides, total cholesterol, low-density lipoprotein cholesterol, alanine transferase (P < 0.01) and bilirubin (P < 0.05), and increased high density lipoprotein cholesterol (P < 0.01). Furthermore, the parameters of oxidative status in plasma were positively influenced by the use of dietary verbascoside, which resulted in significant decreases in reactive oxygen metabolites (P < 0.01) and thiobarbituric acid-reactive substances (P < 0.05) and increases in vitamins A and E (P < 0.01).The use of verbascoside during the peripartum period provided benefits on some blood parameters, oxidative status and milk production, particularly in the immediate postpartum period (0–20 d)

    Effect of dietary Lippia citriodora extract on reproductive and productive performance and plasma biochemical parameters in rabbit does

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    Two doses of natural extract from Lippia citriodora (titrated in verbascoside) were assessed in New Zealand White rabbit does evaluating selected reproductive, productive and plasma biochemical parameters. After 1 week of adaptation period, the trial on 45 rabbit does for three consecutive reproductive cycles was conducted; does were divided into three groups of 15 animals each, homogenous by age (1 year ± 2 weeks), bodyweight (4.77 ± 0.21 kg) and parity (2 ± 1). A control group (CON) did not receive the dietary supplement in the feed and the other two groups received 1 g of natural extract supplement in the feed (5 mg verbascoside/kg feed; LNE) and 2 g of natural extract (10 mg verbascoside/kg feed; HNE). The use of a dietary NE supplement improved kit bodyweight at weaning (934 vs 1104 g; P < 0.001), and average daily weight gain from birth to weaning (24.7 vs 29.7 g/day; P < 0.001), with no NE dose effect. In the LNE and HNE groups serum triglycerides, total cholesterol, low density lipoprotein cholesterol, bilirubin, and activities of alkaline phosphatase, alanine aminotransferase (P < 0.05) and aspartate aminotransferase (P < 0.01) decreased and high density lipoprotein cholesterol (P < 0.01) increased, according to the cycle effect. The dietary supplement also improved blood oxidative status markers in the experimental groups due to an increase in the concentrations of plasma vitamin A and E (P < 0.01) and a decrease in plasma reactive oxygen metabolites and thiobarbituric acid reactive substances values (P < 0.01). In conclusion, the dietary Lippia NE supplement improved selected productive and reproductive parameters and the animal welfare of does, expressed by a general improvement of blood profile, with no effect of the dose

    Going Beyond Counting First Authors in Author Co-citation Analysis

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    The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed

    Effect of dietary natural extracts mixture on rabbit does reproductive performances: preliminary data

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    In the recent years, research has focused the attention on the use of substances able to improve both animal health and meat quality. The objective of this study was to investigate the effect of dietary supplementation with natural extracts in rabbit does on reproductive parameters. Natural extracts have been widely reported to have potent antioxidant, anti-inflammatory and antimicrobic activities related especially to their phenolic content (Pereira et al., 2009). In rabbit production, does are normally inseminated 11 days after parturition, with kits being weaned at 35 days (Rebollar et al., 2006). The reproductive phase is a critical period because the animals have few days to reconstitute their body reserves (Castellini et al., 2003). Therefore, the interaction between nutrition and reproduction is crucial in this specie (Butler, 2000; Roche et al., 2000). The trial was performed at the Research Institute for animal production in Nitra (Slovak Republic). Sixty does were randomly selected, artificially inseminated and divided into three experimental groups. The first fed a basal diet (C), the second one (T1) received 0.3% of natural extracts mixture and the third one (T2) an integration of 0.6% of natural extracts mixture for gestation and lactation period (65 days) The animals were allocated in individual flat-deck cages with a closeable nest box. At parturition, the total number of kits born and stillborn and litters weights were recorded. The data were analyzed by one way Analysis of Variance (ANOVA) using SPSS (IBM.-SSPS Statistics 24). Dietary supplementation did not affect (P>0.05) number of kids born (8.0 ± 1.0 C vs 7.3 ± 0.97 T1 and 7.4 ± 1.0 T2) and birth weight (63 ± 2.0 g C vs 60.1 ± 2.3 g T1 and 61.0 ± 2.4 g T2). The administration of natural extract in does did not improve (P>0.05) the kits average daily gain (20.54 ± 1.3 g/d C vs 21.92 ± 0.5 g/d T1 and 20.93 ± 0.9 g/d T2) and body weight at weaning (829 ± 16.6 g C vs 834 ± 26.6 g T1 and 826 ± 26.8 g T2). These preliminary data showed that at the present dosage, the natural extracts mixture is not able to affect does reproductive performance. However, further research is needed to confirm the present data and explore the mechanism of action of this natural mixture

    Variations on the Author

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    “Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship

    Appropriate Similarity Measures for Author Cocitation Analysis

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    We provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
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