67 research outputs found
Boxer TIM
No full text8 páginas ; 28 cm.Camisería inglesa tiene una trayectoria de 40 años con su prenda insignia las camisas y a lo
largo del camino se han ido incorporando nuevas prendas para poder vestir al hombre
colombiano, ofreciendo elegancia, calidad, estilo y distinción, mediante el diseño, la
fabricación y la comercialización de prendas de vestir masculinas con tecnología de punta y
personal altamente calificado, bajo la premisa del desarrollo y la responsabilidad social.Consideraciones generales.-- Pregunta de investigación.-- Objetivo de investigación.-- Justificación.-- Marco referencial.-- Metodología.-- Resultados.-- Conclusiones.-- Recomendaciones.-- Bibliografía.PregradoDiseñador de ModasDiseño de Moda
Development of fluorinated 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine analogs with potent nigrostriatal toxicity for potential use in positron emission tomography studies
No full textThe discovery of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) toxicity stimulated intense interest in neurotoxicology and in the possible toxic etiology of Parkinson's disease. Better understanding of MPTP neurotoxicity may be achieved by studies using 18F-radiolabeled MPTP analogs and positron emission tomography in nonhuman primates. We synthesized three fluorinated analogs of MPTP: 1-methyl-4-(2-fluorophenyl)-1,2,3,6-tetrahydropyridine (2'-F-MPTP), 1-methyl-4-[2-(trifluoromethyl)phenyl]-1,2,3,6-tetrahydropyridine (2'-CF3-MPTP) and 1-methyl-4-[2-(fluoromethyl)phenyl]-1,2,3,6-tetrahydropyridine (2'-CH2F-MPTP), and developed a method for preparing the latter in 18F-labeled form. We now studied the suitability of 2'-CH2F-MPTP and its hydrolysis products as substrates for monoamine oxidase (MAO) from mouse and monkey brain preparations, and investigated the neurotoxic effect of 2'-CH2F-MPTP and 2'-F-MPTP on the nigrostriatal dopaminergic system in mice. We found that 2'-CH2F-MPTP is a better substrate for MAO and that both 2'-CH2F-MPTP and 2'-F-MPTP were more potent neurotoxins than MPTP. Like MPTP, 2'-F-MPTP was exclusively oxidized by MAO-B and its toxicity blocked by pargyline or deprenyl but not by clorgyline. In contrast, 2'-CH2F-MPTP was oxidized by both MAO-A and MAO-B, and its toxicity was not blocked by pargyline, clorgyline or deprenyl when given separately, but required clorgyline and deprenyl together.PublishedN/
A novel 3' untranslated region mutation in the SLC29A3 gene associated with pigmentary hypertrichosis and non-autoimmune insulin-dependent diabetes mellitus syndrome
No full textBackground
Pigmentary hypertrichosis and non‐autoimmune insulin‐dependent diabetes mellitus (PHID) is one of the rare H syndrome diseases mainly characterized by hyperpigmentation, hypertrichosis, sensorineural hearing loss, cardiac complications, developmental delay, and diabetes mellitus (DM). Mutations in the coding regions of the SLC29A3 gene that encodes for an equilibrative nucleoside transporter (ENT3) have been reported to cause the phenotypic spectrum of the H syndrome. Disease‐causing mutations in the untranslated regions (UTRs) of the SLC29A3 gene have not been previously described in the literature. The aim of the study is to describe and assess the pathogenicity of a novel 3’UTR mutation in the SLC29A3 gene associated with the PHID phenotype in two Turkish patients.
Methods
The mutation was identified by a targeted gene approach. To understand the pathogenicity of this 3’UTR mutation, RNA and protein expression studies were performed by using the quantitative real‐time polymerase chain reaction method and western blotting, respectively, using fibroblasts cultured from the patients' skin biopsies.
Results
SLC29A3 and ENT3 expression levels were both decreased in the patients compared to controls matched for passage numbers, RNA, and protein extraction methods.
Conclusions
A novel 3’UTR mutation in the SLC29A3 gene is associated with the PHID syndrome, highlighting a potentially new pathological mechanism for this disease. The involvement of the 3’UTR has not been previously established in any of the H syndrome disease cluster or in any complex syndrome of DM
Physiological increases in uncoupling protein 3 augment fatty acid oxidation and decrease reactive oxygen species production without uncoupling respiration in muscle cells
No full textDecreased uncoupling protein (UCP)3 is associated with insulin resistance in muscle of pre-diabetic and diabetic individuals, but the function of UCP3 remains unclear. Our goal was to elucidate mechanisms underlying the negative correlation between UCP3 and insulin resistance in muscle. We determined effects of physiologic UCP3 overexpression on glucose and fatty acid oxidation and on mitochondrial uncoupling and reactive oxygen species (ROS) production in L6 muscle cells. An adenoviral construct caused a 2.2- to 2.5-fold increase in UCP3 protein. Palmitate oxidation was increased in muscle cells incubated under normoglycemic or hyperglycemic conditions, whereas adenoviral green fluorescent protein infection or chronic low doses of the uncoupler dinitrophenol had no effect. Increased UCP3 did not affect glucose oxidation, whereas dinitrophenol and insulin treatments caused increases. Basal oxygen consumption, assessed in situ using self-referencing microelectrodes, was not significantly affected, whereas dinitrophenol caused increases. Mitochondrial membrane potential was decreased by dinitrophenol but was not affected by increased UCP3 expression. Finally, mitochondrial ROS production decreased significantly with increased UCP3 expression. Results are consistent with UCP3 functioning to facilitate fatty acid oxidation and minimize ROS production. As impaired fatty acid metabolism and ROS handling are important precursors in muscular insulin resistance, UCP3 is an important therapeutic target in type 2 diabetes. <br/
Potent neurotoxic fluorinated 1‐methyl‐4‐phenyl‐1,2,3,6‐tetrahydropyridine analogs as potential probes in models of parkinson disease
No full textWe synthesized a number of fluorinated analogs of 1‐methyl‐4‐phenyl‐1,2,3,6‐tetrahydropyridine (MPTP), and tested their suitability as substrates for monoamine oxidase B in vitro and their dopaminergic neurotoxicity in vivo. Two of the compounds tested, 2′‐F‐MPTP and 2′‐CF3‐MPTP, were better enzyme substrates and possessed more potent neurotoxicity for nigrostriatal dopamine neurons than MPTP, especially 2′‐F‐MPTP. The results of the in vivo neurotoxicity experiments correlated well with the suitability of the compounds as substrates for monoamine oxidase. These findings could serve as a basis for the use of 18F‐labeled analogs of MPTP for positron emission tomography studies of nonhuman primates for better understanding of the factors underlying MPTP toxicity. Furthermore, the discovery of two MPTP analogs with enhanced selective neurotoxicity to dopaminergic neurons may be an important clue in the continuing efforts to define the chemical structure‐activity factors governing MPTP neurotoxic activation mechanisms.PublishedN/
Environmental Contestation, Planning and Political Change: The Save Bisri Campaign in Lebanon
No full textThough environmental contestation in the Global South is hardly effective (Haynes, 1999), scholars of social and environmental movements have observed new forms of collective action with increased potential for success (MJ, 2017; Haynes, 1999; DeLuca, 1999; Dellaporta and Diani, 2020). These movements are loosely organized and dealing with diverse localized issues, yet increasingly coordinating with each other, joining efforts through multi-scalar networks and coalitions, and building common discourses based on socioeconomic grounds.
The role of urban and regional planners in this regard is crucial. Advocacy planning theorists have highlighted the responsibility of planners in achieving social and environmental justice by redistributing resources, political power, and participation toward disadvantaged groups. However, as planners continue to struggle to challenge the capitalist forces and influence decision-making, they are encouraged to engage directly in politics either through representative democracy processes (Grooms and Boamah, 2018), or within the realm of activism and social movements (Sager, 2016).
This thesis analyzes the environmental campaign to save the Bisri Valley in Lebanon threatened by a World Bank-funded dam project. Coordinated by the author, the campaign emerged in a context where large dams are regarded as symbols of modernization and are subject to sectarian profit-sharing (Riachi, 2016), and where environmental activism is often unable to challenge the clientelist dynamics (Karam, 2006). This thesis argues that the Save the Bisri Valley campaign has been able to threaten the feasibility of the project by elaborating a set of strategies which incorporate advocacy planning and social movement logics. These strategies are distinguished by their multi-scalar and diverse modalities, and by their abilities to mobilize local and international support. Further, the campaign has operated effectively within the political landscape, challenging the state’s power structure and shifting the paradigm that determines the making of water and development policies in Lebanon
Irritantcy potential and sub acute dermal toxicity study of Pistacia Lentiscus fatty oil as a topical traditional remedy
No full textThe current study was undertaken to assess safety of Pistacia lentiscus fruits fatty oil (PLFO) as a topical traditional remedy. A primary skin and eye irritation tests were conducted with New Zealand white rabbits to determine the potential for PLFO to produce irritation from a single application. In addition, a sub acute dermal toxicity study was performed on 18 NZW rabbits to evaluate possible adverse effect following application of PLFO for 28 days. Based on the results of the current study, PLFO is classified as slightly irritating to the skin and the eye of rabbits (Primary Irritation Index (P.I.I.) = 1.037; Ocular Irritation Index (O.I.I.) = 5.33 at 1 h). In the sub-acute toxicity test, PLFO produced neither mortality nor significant differences in the body and organ weights between control group and treated rabbits. However, a reversible irritant contact dermatitis was observed in the treated areas from the end of the second week of application until the end of experiment. This local phenomenon was accompanied by a significant skin thickening (P.0.01) since the 12th day (ANOVA, F = 11, 07143, P = 0, 00765) which is confirmed with an inflammatory granuloma in histological study. Haematological analysis and blood chemistry values of the 2 groups showed no significant differences in any of the parameters examined. In summary, PLFO is minimally irritating to the eye and skin after a single exposure, but it may cause irritant contact dermatitis and a reversible thickening of skin after prolonged use.Key words: Pistacia lentiscus, fatty oil, skin, eye, dermatitis, irritation, toxicity
Avaliação da recusa e suspensão de terapêutica(RST) em pacientes internados na Unidade de Terapia Intensiva (UTI) de um Hospital Universitário.
No full textTrabalho de Conclusão de Curso - Universidade Federal de Santa Catarina. Curso de Medicina. Departamento de Clínica Médica
Effect Of Virgin Fatty Oil Of Pistacia Lentiscus On Experimental Burn Wound&Apos;S Healing In Rabbits
No full textThis study aimed to assess the efficiency of the virgin fatty oil of
Pistacia lentiscus (PLVFO) for burn wounds healing. It was carried out
on 6 adult male New Zealand rabbits. Four burn wounds of deep third
degree were made on the back of each animal. The first was not treated
and served as control (CRL group); the others were covered immediately
after burning procedure by 0.5g of one of the following products:
Vaseline gel (VAS group), Madecassol® cream 1% (MAD group) or 1ml
of PLVFO (PLVFO group). The treatments were repeated once daily until
complete healing. For four days post burns, the percentage of wound
contraction was assessed. Also, the different healing times were noted.
The results showed that both PLVFO and Madecassol® significantly
accelerated wound healing activity compared to wounds dressed with
Vaseline and the untreated wounds. However, the level of wound
contraction was significantly higher and the healing time was faster in
PLVFO group than those of the MAD group, VAS group and CRL group. The
different epithelization periods obtained in days were respectively:
30±3.94 (PLVFO group), 33.5±3.78 (MAD group), 34.66±3.88
(VAS group) and 37.16±3.54 (CRL group). We conclude that Pistacia
lentiscus virgin fatty oil promotes significantly (p< 0.05) wound
contraction and reduces epithelization period in rabbit model
Design and synthesis of 18F-labeled neurotoxic analogs of MPTP
No full textWe report on the synthesis of two fluorine-18 labeled analogs of l-methyl-4-phenyl-1, 2, 3, 6- tetrahydropyridine (MPTP). A piperidyl triazene was fluorinated to produce [18F]-l-methyl-4- (2-fluorophenyl)-l, 2, 3, 6-tetrahydropyridine (2'-F-MPTP, 12) in very low yield, and l-methyl-4-[2-(fluoromethyl) phenyl]-1, 2, 3, 6-tetrahydropyridine (2'-FCH2-MPTP, 11) was labeled with 18F by nucleophilic displacement of the corresponding chloride in 60% yield. The biodistribution in mice of the latter radiotracer and its oxidation to l-methyl-4-[2-(fluoromethyl)phenyl]pyridinium (2'-FCH2-MPP+, 6) is also reported. The kinetics of oxidation of 2'-FCH2-MPTP and its solvolysis products (the corresponding 2'-hydroxymethyl and 2'-chloromethyl analogs) by rat liver monoamine oxidase were investigated. 2'-FCH2-MPTP accumulated to a useful degree in the brain, was oxidized by monoamine oxidase in vitro, was converted to the oxidation product in brain in vivo, and had a neurotoxic potency similar to that of MPTP. We feel it may be useful as an 18F-labeled radiopharmaceutical for positron tomographic studies of the mechanisms of MPTP toxicity.PublishedN/
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