2,536 research outputs found
Progetti per Milano. Concorso di idee per il polo direzionale-finanziario nell'area Garibaldi Repubblica
No full textIl volume contiene il progetto di concorso per l'area Garibaldi Repubblica del gruppo coordinato da G. Polesell
Identification of race 1 of Fusarium oxysporum f. sp. lactucae on lettuce by inter-retrotransposon sequence-characterized amplified region technique
No full textFusarium wilt of lettuce, caused worldwide by Fusarium oxysporum f. sp. lactucae, is an emerging seed-transmitted disease on Lactuca sativa. In order to develop a molecular diagnostic tool for identifying race 1 (VCG0300) of the pathogen on vegetable samples, an effective technique is presented. Inter-retrotransposon amplified polymorphism polymerase chain reaction (PCR), a technique based on the amplification of genomic regions between long terminal repeats, was applied. It was shown to be useful for grouping F. oxysporum f. sp. lactucae race 1 isolates. Interretrotransposon sequence-characterized amplified regions (IR-SCAR) was used to develop a specific set of PCR primers to be utilized for differentiating F. oxysporum f. sp. lactucae isolates from other F. oxysporum isolates. The specific primers were able to uniquely amplify fungal genomic DNA from race 1 isolates obtained in Italy, Portugal, the United States, Japan, and Taiwan. The primers also were specific to pathogen DNA obtained from artificially infected lettuce seed and naturally and artificially infected plants
La Divisione italiana partigiana Garibaldi in Montenegro (1943-1945)
No full textVicende della Divisione italiana partigiana Garibaldi in Montenegro tra il 1943 e il 194
La leggenda del pioniere. Diario Mazzini Garibaldi Pissard (1929-1983)
No full textIl volume presenta le memorie di Mazzini Garibaldi Pissard (1908-1997), tecnico della perforazione dell'Agip, in servizio presso l'azienda petrolifera di stato dagli anni venti agli anni ottanta. Il diario è corredato da un'introduzione storica (D. Pozzi), dai ricordi del figlio Paolo Pissard e da note biografiche (F. Guidi
Silvia Cavicchioli, Anita. Storia e mito di Anita Garibaldi, Torino, Einaudi, 2017
No full textRecensione del volume di Silvia Cavicchioli "Anita. Storia e mito di Anita Garibaldi" (Torino, Einaudi, 2017).Review of Silvia Cavicchioli's book "Anita. Storia e mito di Anita Garibaldi" (Torino, Einaudi, 2017)
Vegetative compatibility groups of Fusarium oxysporum f. sp. lactucae from lettuce
No full textFusarium oxysporum f. sp. lactucae, the causal agent of Fusarium wilt of lettuce, has been reported in three continents in the last 10 years. Forty-seven isolates obtained from infected plants and seed in Italy, the United States, Japan, and Taiwan were evaluated for pathogenicity and vegetative compatibility. Chlorate-resistant, nitrate-nonutilizing mutants were used to determine genetic relatedness among isolates from different locations. Using the vegetative compatibility group (VCG) approach, all Italian and American isolates, type 2 Taiwanese isolates, and a Japanese race 1 were assigned to the major VCG 0300. Taiwanese isolates type 1 were assigned to VCG 0301. The hypothesis that propagules of Fusarium oxysporum f. sp. lactucae that caused epidemics on lettuce in 2001-02 in Italian fields might have spread via import and use of contaminated seeds is discussed
PCR detection of Fusarium oxysporum f. sp. basilici on basil
No full textSixty-nine amplified DNA fragments, generated from different isolates of Fusarium oxysporum
f. sp. basilici, were tested for F. oxysporum f. sp. basilici–specificity in a dot blot assay. One
1,038-bp fragment hybridized to DNA from all F. oxysporum f. sp. basilici isolates but not to
DNA obtained from F. oxysporum isolates nonpathogenic to basil or representatives of other
formae speciales of F. oxysporum, or from isolates of F. redolens, F. tabacinum, Rhizoctonia
solani, Sclerotinia sclerotiorum, S. minor, and Pythium ultimum obtained from diseased basil.
This fragment was cloned and sequenced, and three pairs of F. oxysporum f. sp. basilici–
specific primers were designed, giving rise to amplification products of 943, 382, and 330 bp. A
nested PCR assay allowed detection of F. oxysporum f. sp. basilici in diseased seedlings and in
artificially and naturally contaminated seeds. The theoretical detection limit of this system was
102 fungal propagules per 100 seeds on artificially contaminated samples, while on naturally
contaminated commercial seed lots, 32 propagules per 100 seeds were detected
Strumenti di valutazione dell’eco-efficienza
No full textPartendo dalla definizione del problema ambientale si sono analizzati i principali strumenti dell'eco-efficienza a livello micro e macro-economic
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