2,388 research outputs found
Dimer-dimer stacking interactions are important for nucleic acid binding by the archaeal chromatin protein Alba
No full textArchaea use a variety of small basic proteins to package their DNA. One of the most widespread and highly conserved is the Alba (Sso10b) protein. Alba interacts with both DNA and RNA in vitro, and we show in the present study that it binds more tightly to dsDNA (double-stranded DNA) than to either ssDNA (single-stranded DNA) or RNA. The Alba protein is dimeric in solution, and forms distinct ordered complexes with DNA that have been visualized by electron microscopy studies; these studies suggest that, on binding dsDNA, the protein forms extended helical protein fibres. An end-to-end association of consecutive Alba dimers is suggested by the presence of a dimer-dimer interface in crystal structures of Alba from several species, and by the strong conservation of the interface residues, centred on Are and Phe(60). In the present study we map perturbation of the polypeptide backbone of Alba upon binding to DNA and RNA by NMR, and demonstrate the central role of Phe(60) in forming the dimer dimer interface. Site-directed spin labelling and pulsed ESR are used to confirm that an end-to-end, dimer dimer interaction forms in the presence of dsDNA.Peer reviewe
Vorticella alba Fromentel 1874
No full textVorticella alba Fromentel, 1874 Known Distribution. Switzerland. Hosts. Macrocyclops albidus (cited as Cyclops albidus); Cyclops viridis; Gammarus pulex. References. Keiser 1921; Morado & Small 1995.Published as part of Mayén-Estrada, Rosaura & Pinto Utz, Laura R., 2018, A checklist of species of Vorticellidae (Ciliophora: Peritrichia) epibionts of crustaceans, pp. 301-328 in Zootaxa 4500 (3) on page 311, DOI: 10.11646/zootaxa.4500.3.1, http://zenodo.org/record/529765
First person – Alba Delrio-Lorenzo
No full textFirst Person is a series of interviews with the first authors of a selection of papers published in Journal of Cell Science, helping early-career researchers promote themselves alongside their papers. Alba Delrio-Lorenzo is first author on ‘Sarcoplasmic reticulum Ca2+ decreases with age and correlates with the decline in muscle function in Drosophila’, published in JCS. Alba is a PhD student in the lab of Javier García-Sancho and María Teresa Alonso at the Instituto de Biología y Genética Molecular (IBGM), University of Valladolid, Spain, investigating the molecular mechanisms implicated in aging, particularly muscle aging.Peer reviewe
The Author/Translator Interactional Process. A Case Study
No full textSee Naples and Kill (1988) is a lively and colourful novel by the con-temporary English writer, Gregory Dowling, translated into Italian in 2015.
Following the tradition of translation studies (Venuti 2000, Bass-nett 2002, Cronin 2006), this paper analyses the rewriting process of literary translation, considering in particular the fruitful but sometimes tense and even conflictual relationship between writer and translator.
The translation of the novel See Naples and Kill was an ongoing rewriting process entailing a constant dialogue between the writer and the translator. Therefore, the study aims at answering two main ques-tions: what happens if the rewriting process of translation is constant-ly questioned by the author? What happens if the author has a good mastery of the target language and s/he is her/himself a translator?
By exploring the relationship between translation and re-creation, the research focuses on the differences and similarities between the primary creation (source text) and the secondary creation (target text), and aims to verify in which way the dialogic encounter of two different personalities and cultures does not make them merge but, by retaining their own uniqueness, leads eventually to their mutually en-riching each other. A comparative analysis of the source text and the different drafts of the translated version accompanied by the author’s comments will shed light on the tense author-translator relationship in the specific case under investigation and how both actors handle this tension in order to create a new work resulting from the (dis)agreement of the two parties
Assessment of the National Park network of mainland Spain by the Insecurity Index of vertebrate species
No full textData set of the above mentioned paper. Distribution of 37 vertebrate species and values of environmental variables in each 10 km x 10 km UTM cell of mainland Spain. Codes for the species and for the variables are detailed in Table 1 and Table S1 of the paper, respectively. IDcuad and UTM10 are numerical and text identifiers of the cells. NP details if the cell overlaps with a National Park. Proportion_NP is the proportion of the cell covered by National Parks.Distribution of 37 vertebrate species and values of environmental variables in each 10 km x 10 km UTM cell of mainland Spain.The study was funded by Organismo Autónomo Parques Nacionales of Spain through the project 1098/2014N
Práctica empresarial en la personería municipal de Chimá, Córdoba
No full textEl presente informe tuvo como objeto abordar el desarrollo de la práctica empresarial realizada en la Personería Municipal de Chimá, entidad encargada de la promoción y protección de los derechos fundamentales, así como de la vigilancia de la gestión pública en el municipio. El propósito principal de la práctica fue brindar apoyo en el área jurídica de la entidad, con el fin de contribuir al fortalecimiento institucional y a la mejora en la garantía y protección oportuna de los derechos de la ciudadanía, a través de una atención más eficaz y eficiente en los trámites legales.1. CONSTANCIA DE APROBACIÓN DE TUTORES 32. DEDICATORIA 4RESUMEN 6ABSTRACT 73. INTRODUCCIÓN 84. OBJETIVOS DE LA PRÁCTICA EMPRESARIAL 105. CAPÍTULO I: DESCRIPCIÓN DE LA ENTIDAD 115.1 Nombre de la institución 115.2 Misión y Visión 125.3 Estructura organizacional 135.4 Actividades de la entidad 146. CAPÍTULO II DESCRIPCIÓN DE LA DEPENDENCIA DONDE REALIZÓ LA PRACTICA EMPRESARIAL 156.1 Organigrama 166.2 Funciones 167. CAPÍTULO III ACTIVIDADES REALIZADAS DURANTE LA PRÁCTICA EMPRESARIAL 207.1 Descripción del problema 207.2 Plan de Trabajo 217.3 Cronograma de actividades 17.4 Descripción detallada de las actividades 18. CONCLUSIONES 49. PROPUESTA 710. IMPACTO DE LA PRÁCTICA EMPRESARIAL 1011. REFERENCIAS BIBLIOGRÁFICAS 1212. ANEXOS 15Anexo 1. Acta de posesión No. 001 mediante la cual se vincula a la estudiante Alba Ruiz Villadiego como practicante a la personería Municipal de Chimá , Córdoba. 15Anexo 2. Certificado de afiliación ARL estudiante Alba Ruiz Villadiego. 16Anexo 3. Eviedencia fotogracfica . 17Anexo 4. Evidencia fotografica. 18Anexo 5. Informe del mes de diciembre de 2024 sobre el Desarrollo de la Práctica Empresarial de Alba Ines Ruiz Villadiego en la personería municipal de Chimá, Córdoba. 19Anexo 6. Informe del mes de enero de 2025 sobre el Desarrollo de la Práctica Empresarial de Alba ines Ruiz Villadiegoen la personería municipal de Chimá, Córdoba. 20Anexo 7. Informe del mes de Feberero de 2025 sobre el Desarrollo de la Práctica Empresarial de Alba Ines Ruiz Villadiego en la personería municipal de Chimá, Córdoba. 21Anexo 8. Informe del mes de Marzo de 2025 sobre el Desarrollo de la Práctica Empresarial de Alba Ines Ruiz Villadiego en la personería municipal de Chimá, Córdoba. 22PregradoAbogado(a)Práctica Empresaria
Hornschuchia alba
No full textHornschuchia alba (A.St.-Hil.) R.E.Fr. Fig. 1 Acta Horti Bergiani 10 (2): 137 (Fries 1931). Basionym Bocagea alba A.St. -Hil., Flora Brasiliae meridionalis 1 (2): 42 (Saint-Hilaire 1825). Type: BRAZIL – Rio de Janeiro • “ Cabo Frio, Ponta de lʼEst au Cap Frio, Prov. Rio de Janeiro ”; A. Saint-Hilaire 366; holotype: P [P032535]!; isotypes: MPU [MPU026907]!, S [S-R-7077]! (fragment). Material examined BRAZIL – Rio de Janeiro • “ Armação dos Búzios, Praia Brava. Mata de Encosta Sul voltada para o costão da Praia Brava ”; 24 May 2001; C. Farney 4371; RB [RB00451923]!, SPF! • “ Costão para João Fernandes. Mata de encosta Praia Brava- Costão para João Fernandes ”; [22°44′38.5″ S, 41°52′17.8″ W]; 23 Aug. 2001; C. Farney 4397; RB [RB00452010]!, SPF! • “ Encosta da Praia Brava ”; 22°44′92″ S, 41°52′40″ W; 18 Feb. 2004; H.G. Dantas 620; RB [RB00086462]! • ibid.; 22°44′92″ S, 41°52′40″ W; 18 Feb. 2004; H.G. Dantas 156; RB [RB00086467]! • “ Rancho Dez ”; Jun. 1997; C. Farney s.n.; RB [RB00372099]! • “ Fazenda Caravelas ”; alt. 80 m; 20 Oct. 1999; C. Farney 3930; SPF!, RB [00452151]! • “ Estrada Cabo Frio- Búzios, entre a Serra das Esmerencias e a Praia de José Gonçalves ”; [22°48′32.8″ S, 41°56′37.8″ W]; 5 Jun. 1998; J.M.A. Braga 4871; RB [RB00042085]! • “ Fazenda of José Gonçalves ”; 22°47′ S, 41°57′ W; alt. 120 m; 8 Feb. 1999; P.J. Maas 8818; NY [NY02699049]!, RB [RB00042092]!, SPF! • “ Estrada antiga para Búzios, próximo ao centrinho, ramal da lixeira ”; 28 Jun. 1995; D. Araújo 10320; RB [RB00417332]! • “ Cabo Frio, Morro da Piaçava ”; 9 Oct. 2002; C. Farney 4487; SPF!, RB [RB00451944]! • “ Morro do Mico ”; [22°51′45.4″ S, 42°00′38.5″ W]; 22 Jun. 2002; C. Farney 4463; RB [RB00451806]!, SPF! • 27 Aug. 1997; C. Farney 3587; CEPEC [CEPEC00118741]!, MBM [MBM325079]!, R [RB00043047]!, SPF!. – Locality unknown • “ E Brasilia ”; Freyreis s.n.; S n.v. Description Shrubs, rarely treelets, 2–7 m tall. Leaves chartaceous; petiole 1–3 mm long; lamina 3.8–9.3 × 1.9–4.9 cm, narrowly ovate to ovate or lanceolate, both surfaces glabrous, base acute to obtuse, apex acuminate to acute, obtuse or rounded; primary vein impressed adaxially and raised abaxially, 6–12 pairs of secondary veins, angles between primary and secondary veins 50–60°. Inflorescence one-flowered; supra-axillary, terminal or leaf-opposed, bracts absent. Flowers with pedicel 1–7 mm long; flower buds conical to cylindrical with obtuse apex, 2–8 × 1–3 mm, covered in trichomes. Sepals completely connate, calyx cupuliform, apex truncate, 2 × 2–3 mm, densely covered in trichomes. Petals linear, white, 11–14 mm long, covered in trichomes; stamens 6, ca 2 × 0.5 mm; carpels 3, ca 3 × 0.5 mm. Monocarps 2, immature monocarps fusiform, 4–9 × 3 mm, densely covered in trichomes, mature monocarps obovate, ca 2 cm long (Saint-Hilaire 1825); stipe ca 1.5 mm long, calyx persistent. Seed ca 6 mm long, ellipsoid, reddish, with fleshy aril (Saint-Hilaire 1825). Distribution and habitat Hornschuchia alba is endemic to Rio de Janeiro State. It is known from only two municipalities, Armação de Búzios and Cabo Frio, occurring in seasonal semideciduous lowland forest (Mello-Silva et al. 2021; Fig. 1). Phenology Flowering from February to October, fruiting from September to October. Preliminary conservation status Critically Endangered, CR B1ab(i,ii,iii) (Amaro 2016). At the time of the last revision of the genus (Johnson & Murray 1995), only two historical collections of H. alba, including the type, were available. The species was only known with certainty from Cabo Frio. Nowadays there are eight localities, in Armação de Búzios and Cabo Frio, where the species is known to occur. However, H. alba has not been collected in 18 years. Notes Johnson & Murray (1995) indicated the holotype as Saint-Hilaire 99. However, this collection number has been assigned to a species of Amaranthaceae Juss. The holotype of Bocagea alba is in fact Saint-Hilaire 366 (Saint-Hilaire’s field notebooks are available at http://hvsh.cria.org.br/works). There are only immature fruits available (Farney 3930 and 4487), the fruit and seed descriptions have been complemented with the information in Saint-Hilaire (1825). With the new collections of H. alba, the description of the species has been updated (Johnson & Murray 1995) with new information about the habit and plant size, inflorescence position and flower dimension. Hornschuchia alba has a single-flowered inflorescence, supra-axillary, terminal or leaf-opposed. It is similar to the specimens of H. myrtillus with this type of inflorescence. However, H. alba is different from H. myrtillus in the leaves that are narrowly ovate to ovate or lanceolate (vs narrowly elliptic to elliptic, oblanceolate to narrowly oblong, obovate or rarely lanceolate) and the pedicel 1–7 mm long (vs 10–28 mm long).Published as part of Vilela, Lucas & Lopes, Jenifer De Carvalho, 2022, Hornschuchia (Annonaceae), an endemic and threatened genus from the Brazilian Atlantic Forest, pp. 75-108 in European Journal of Taxonomy 828 on pages 79-80, DOI: 10.5852/ejt.2022.828.1859, http://zenodo.org/record/682376
Reduction of Setup Times in a Metal Fabrication Company Using a Lean-Sigma Approach
No full textManufacturing companies face demand fluctuations and must deliver the required quantity of parts to their customers within the required time; however, companies face different challenges in fulfilling them. One is technological advances, which make it impossible for them to compete with other countries, such as China, due to financial issues. Therefore, manufacturing companies use continuous improvement methodologies to achieve better performance in their processes. Six Sigma and Lean Manufacturing are some of the best-known approaches. This article briefly presents the application of a lean sigma approach oriented to solving problems. This research proposes a methodology based on Lean Sigma that contrasts what different authors currently do: continuous improvement projects that take up to 12 months to produce results and do not solve a problem. This implementation is conducted in a process that manufactures metal parts and fails to deliver them on time to its customers. Using tools such as the Single Minute Exchange of Die (SMED) in a production line, on-time deliveries were increased from 89 % to 96 %; at the same time, the model change times were reduced by approximately 60 %, which is required for the implementation of this methodology for only four weeks, unlike the traditional six-sigma approach that can take up to 12 months to improve the process
A ALBA e a luta pela hegemonia no continente americano: uma topologia do discurso bolivariano
No full textDissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Filosofia e Ciências Humanas. Programa de Pós-graduação em Sociologia PolíticaO objetivo da dissertação é analisar o movimento bolivariano, liderado pela Venezuela, enquanto projeto de construção de uma nova hegemonia na América Latina e o Caribe. Tal projeto se constrói discursivamente em oposição à histórica hegemonia pan-americana liderada pelos Estados Unidos (EUA). Para isso é estudada a ALBA (Aliança Bolivariana para os Povos de nossa América) como organização internacional que facilita a alineação de países e sociedades latino-americanos em relação ao movimento bolivariano, gerando novas modalidades de construção de sentido, assim como novas vias e regras de relacionamento internacional. A pesquisa analisa os discursos de mandatários nas reuniões de Cúpula da ALBA, e, apelando à teoria do discurso de Ernesto Laclau, identifica nesse conjunto os três passos previstos pelo autor na configuração de um desafio hegemônico: (1) o estabelecimento de um antagonismo face ao imperialismo dos EUA que é identificado como o poder hegemônico institucionalizado, e a dicotomização do espaço social americano através da construção de uma fronteira entre esse poder e o resto da comunidade (América Latina e o Caribe); (2) a articulação contingente entre diversas demandas sociais, características da América Latina e do Caribe, cuja insatisfação é atribuída ao imperialismo dos EUA; e (3) a formação de um campo popular, através da expressão positiva dessas demandas numa nova identidade, no caso a identidade bolivariana. A pesquisa conclui que o movimento bolivariano consegue gerar elementos para permitir uma nova lógica de construção de sentido, configurando assim estruturalmente um campo de luta pela hegemonia. Porém, também são destacados vários problemas nessa tarefa, especialmente ligados à identificação de diversas unidades com o bolivarianismo.The dissertation research the Bolivarian Movement, headed by Venezuela, as a new hegemonic project, still under construction, at Latin America and the Caribbean. This hegemonic project is discursively constructed in opposition to the historic Pan American hegemony, led by the U.S. In this way, the Bolivarian Alliance for the Peoples of Our America (ALBA) is approached as an international organization that seeks to align the Latin American countries and societies behind the Bolivarian Movment. The ALBA generates new ways of sense-making, as well as new norms of international relations. The research analyzes the speeches at the ALBA's presidential summits, using the Ernesto Laclau's Discourse Theory, and identifying the three steps proposed by this author to the hegemonic construction: Firstly, the establishment of an antagonism against U.S. imperialism and the dicotomization of the hemispheric social space, through the definition of a boundary between the powerful country and the rest of continental community (Latina America and the Caribe). Secondly, the contingent articulation of a variety of social demands, which dissatisfaction are ascribed to the U.S. imperialism. Thirdly, the formation of a new popular field, through the positive expression on a new identity of this unified set of demands, in this case the Bolivarian identity. The dissertation concludes that the Bolivarian movement manages a new logic of sense-making, and therefore places the hegemonic struggle in a new structural field. However, it also identified some problems on this task, particularly in the difficulty to construct a Bolivarian identity shared by various social unities (countries, societies and social sectors)
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