306,550 research outputs found
The life and work of the Revd. T.H.E.C. Espin, perpetual curate of Tow Law, with special reference to his astronomical research
No full textNo abstract supplie
Professores analógicos / professores digitais: a prática docente frente às inovações tecnológicas
No full textDissertação (mestrado) - Universidade Federal de Santa Catarina, Centro Tecnológico. Programa de Pós-Graduação em Engenharia de Produção.Este trabalho visa analisar a efetividade e a eficácia da implementação de um projeto de informática aplicada à educação desenvolvida pela Secretaria Municipal de Educação - SEME, de Brusque - SC, a partir de 2001, chamado ESPIN (Espaço Pedagógico Informatizado). De modo a realizar uma análise mais coerente, este trabalho parte da conceituação das tecnologias de informática e de comunicação na educação, com ênfase nas implicações desta mudança para os professores, quando da sua introdução nas escolas. Algumas experiências mundiais e a brasileira são discutidas e comparadas, de modo a apresentar, de forma mais ampla, o contexto onde o projeto analisado está inserido. O ESPIN é um espaço pedagógico implantado em algumas escolas municipais, equipado com recursos tecnológicos de informática e comunicação, como PCs, scanners, gravadoras de CD-Rom, digitalizadoras de vídeo e som, TV e acesso à Internet. Colocado à disposição dos professores de sala de aula e alunos, com a mediação de professores motivadores, o ESPIN visa o desenvolvimento das atividades curriculares, buscando um novo paradigma didático-pedagógico. A pesquisa foi realizada com 44 professores de 1ª a 4ª séries do ensino fundamental, em cinco escolas da Rede Municipal de Brusque, com ênfase na investigação dos fatores que influenciam ou inibem as práticas dos professores quanto à utilização da estrutura tecnológica disponível no ESPIN. Os dados foram coletados através da aplicação de questionários estruturados e sofreram análises qualitativas e quantitativas. O resultado da pesquisa aponta para as excelências e falhas detectadas na estrutura do projeto, analisando-se como ponto focal o professor de sala de aula e a sua efetiva utilização dos recursos disponíveis. O trabalho analisa o sentimento, os anseios e as percepções dos professores de sala de aula, no que diz respeito à capacitação, disponibilidade, apoio instrucional e emocional. O instrumento elaborado e analisado pode ser utilizado pela coordenação do ESPIN para avaliar escolas com o projeto já implantado e outras que aspiram a implantação do mesmo, gerando um indicador de uso
Code for Analyses and Figures in Espin et al.
No full textCode for Analyses and Figures in Espin et al
T.-E. Espin — Rapport sur les travaux de l’Observatoire Wolsingham pour 1892
No full textC. O. T.-E. Espin — Rapport sur les travaux de l’Observatoire Wolsingham pour 1892. In: Bulletin astronomique, tome 10, 1893. pp. 108-109
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Image2_Studies of functional properties of espin 1: Its interaction to actin filaments.TIF
No full textActin is a multifunctional biomolecule that forms not only basic structural bodies such as filopodia and lamellipodia, but also large microvilli-like organelles like stereocilia. Actin consists of four sub-domains (S1, S2, S3, and S4), and the “target-binding groove” formed between S1 and S3 is the major binding site for various actin binding proteins. Actin filament dynamics are regulated by numerous actin binding proteins with different mechanisms of actin binding, assembly, and disassembly such as actin severing, branching, and bundling. Ectoplasmic specialization protein 1 (espin 1) is an actin binding and bundling protein that is specifically implicated in the elongation and stabilization of stereocilia as a binding partner with myosin III. However, little is known about the molecular structure, actin bundling, and stabilizing mechanism of espin 1; hence, we investigated the interaction between actin and espin 1 through structural data. In this study, we first purified human espin 1 in an E. coli system following a new detergent-free approach and then demonstrated the 2D structure of full-length espin 1 using transmission electron microscopy along with Nickel nitrilotriacetic acid nanogold labeling and 2D averaging using SPIDER. Furthermore, we also determined the espin 1 binding domain of actin using a co-sedimentation assay along with gelsolin and myosin S1. These findings are not only beneficial for understanding the actin binding and bundling mechanism of espin 1, but also shed light on its elongation, stabilization, and tip-localization mechanisms with myosin III. This study thus provides a basis for understanding the molecular structure of espin 1 and can contribute to various hearing-related diseases, such as hearing loss and vestibular dysfunction.</p
Image3_Studies of functional properties of espin 1: Its interaction to actin filaments.JPEG
No full textActin is a multifunctional biomolecule that forms not only basic structural bodies such as filopodia and lamellipodia, but also large microvilli-like organelles like stereocilia. Actin consists of four sub-domains (S1, S2, S3, and S4), and the “target-binding groove” formed between S1 and S3 is the major binding site for various actin binding proteins. Actin filament dynamics are regulated by numerous actin binding proteins with different mechanisms of actin binding, assembly, and disassembly such as actin severing, branching, and bundling. Ectoplasmic specialization protein 1 (espin 1) is an actin binding and bundling protein that is specifically implicated in the elongation and stabilization of stereocilia as a binding partner with myosin III. However, little is known about the molecular structure, actin bundling, and stabilizing mechanism of espin 1; hence, we investigated the interaction between actin and espin 1 through structural data. In this study, we first purified human espin 1 in an E. coli system following a new detergent-free approach and then demonstrated the 2D structure of full-length espin 1 using transmission electron microscopy along with Nickel nitrilotriacetic acid nanogold labeling and 2D averaging using SPIDER. Furthermore, we also determined the espin 1 binding domain of actin using a co-sedimentation assay along with gelsolin and myosin S1. These findings are not only beneficial for understanding the actin binding and bundling mechanism of espin 1, but also shed light on its elongation, stabilization, and tip-localization mechanisms with myosin III. This study thus provides a basis for understanding the molecular structure of espin 1 and can contribute to various hearing-related diseases, such as hearing loss and vestibular dysfunction.</p
Structural and Viscoelastic Properties of Actin Networks Formed by Espin or Pathologically Relevant Espin Mutants
No full textThe structural organization of the cytoskeleton determines its viscoelastic response which is crucial for the correct functionality of living cells. Both the mechanical response and microstructure of the cytoskeleton are regulated on a microscopic level by the local activation of different actin binding and/or bundling proteins (ABPs). Misregulations in the expression of these ABPs or mutations in their sequence can entail severe cellular dysfunctions and diseases. Here, we study the structural and viscoelastic properties of reconstituted actin networks cross-linked by the ABP espin and compare the obtained network properties to those of other bundled actin networks. Moreover, we quantify the impact of pathologically relevant espin mutations on the viscoelastic properties of these cytoskeletal networks
Espin (Rev. T.-E.). — La distribution des étoiles du type III et des spectres stellaires dans l’espace.
No full textEspin (Rev. T.-E.). — La distribution des étoiles du type III et des spectres stellaires dans l’espace.. In: Bulletin astronomique, tome 11, 1894. p. 542
Image1_Studies of functional properties of espin 1: Its interaction to actin filaments.TIF
No full textActin is a multifunctional biomolecule that forms not only basic structural bodies such as filopodia and lamellipodia, but also large microvilli-like organelles like stereocilia. Actin consists of four sub-domains (S1, S2, S3, and S4), and the “target-binding groove” formed between S1 and S3 is the major binding site for various actin binding proteins. Actin filament dynamics are regulated by numerous actin binding proteins with different mechanisms of actin binding, assembly, and disassembly such as actin severing, branching, and bundling. Ectoplasmic specialization protein 1 (espin 1) is an actin binding and bundling protein that is specifically implicated in the elongation and stabilization of stereocilia as a binding partner with myosin III. However, little is known about the molecular structure, actin bundling, and stabilizing mechanism of espin 1; hence, we investigated the interaction between actin and espin 1 through structural data. In this study, we first purified human espin 1 in an E. coli system following a new detergent-free approach and then demonstrated the 2D structure of full-length espin 1 using transmission electron microscopy along with Nickel nitrilotriacetic acid nanogold labeling and 2D averaging using SPIDER. Furthermore, we also determined the espin 1 binding domain of actin using a co-sedimentation assay along with gelsolin and myosin S1. These findings are not only beneficial for understanding the actin binding and bundling mechanism of espin 1, but also shed light on its elongation, stabilization, and tip-localization mechanisms with myosin III. This study thus provides a basis for understanding the molecular structure of espin 1 and can contribute to various hearing-related diseases, such as hearing loss and vestibular dysfunction.</p
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