181 research outputs found
Wokół problemów interpretowania przekazu źródeł numizmatycznych [rec. E. Manders: Coining Images of Power. Patterns in the Representation of Roman Emperors on Imperial Coinage, A.D. 193–284. Leiden–Boston 2012]
No full textArtykuł recenzyjny monografii Erik Manders pt. "Coining Images of Power. Patterns in the Representation of Roman Emperors on Imperial Coinage, A.D. 193—284" (Leiden—Boston 2012)
Wokół problemów interpretowania przekazu źródeł numizmatycznych [recenzja]
No full textReview article of Erik Manders\u27 monograph entitled Coining Images of Power. Patterns in the Representation of Roman Emperors on Imperial Coinage, A.D. 193—284" (Leiden—Boston 2012).Artykuł recenzyjny monografii Erik Manders pt. "Coining Images of Power. Patterns in the Representation of Roman Emperors on Imperial Coinage, A.D. 193—284" (Leiden—Boston 2012)
Unraveling Intermediate Filaments: The super resolution solution
No full textIntermediate Filaments (IFs) carry out major functions in cells. Several diseases have been associated with malfunctioning IFs in the cells and among them are certain sub types of cancer. To determine the structure and organization of IFs, we have used Single Molecule Localization Microscopy (SMLM) . We have shown that keratin IF plays a key role in Hemidesmosomes (HDs) organization in cultured keratinocytes. In addition, we have studied the orientational alignment between vimentin, another abundant IF protein, and MT. With SR, we have observed spatial proximity between single vimentin and MT filaments and we have quantitatively shown that interaction between vimentin and MT are cell-type dependent. Furthermore, we have addressed several critical issues in SMLM imaging. First, we have developed a new buffer which supports multi-color imaging. OxEA, an Oxyrase based imaging buffer, elevates localization precision for commonly used dyes and enhances blinking of other dyes. Second we have improved a dedicated chamber for SR imaging, so called Oxygen Tight Chamber (OTC). In the OTC, we have minimized drift by introducing an alternative curing procedure that reduces stress in the bond between glass and plastic. In brief, in this thesis we have optimized imaging condition and post processing analysis for SMLM microscopy. With the resulting high quality SR images and precise quantification methods, we have contributed to the biological knowledge in the IF field
Application of direct analysis to pulsating and oscillating phenomena
No full textElectrical Engineering, Mathematics and Computer Scienc
Re-scan confocal microscopy
No full textOne of the instruments that gave insight in the morphology and function of cellular components is the optical microscope. Nowadays, optical microscopy in biomedical applications is commonly combined with fluorescence. One fundamental limit in the possibility to distinguish small structures in the sample in fluorescence microscopy is limited by the diffraction of light. Objects that are closer together than the diffraction limit cannot be distinguished. This thesis describes a new super-resolution technique, Re-scan Confocal Microscopy (RCM) which has 1.4 times higher resolution than the diffraction limit. RCM uses a sensitive camera for detection, and therefore combines high resolution with high detection sensitivity. This thesis describes the method in detail and the theory behind the technology. We present the method for the first time, show the proof of principle and characterize its imaging properties. RCM can be used for a wide range of biomedical applications. RCM has been tuned to image multicolor samples and to perform functional studies for example in HeLa cells, yeast cells and neurons. The RCM technique has been combined with spatially-controlled illumination (SCIM) in order to minimize the illumination of the sample for reduction of phototoxicity. In this thesis it is proven that the RCM microscope is a valid alternative to standard confocal microscopy for a variety of biomedical applications where high resolution is required in combination with high sensitivity
Distinct structural plasticity in the hippocampus and amygdala of the middle-aged common marmoset (Callithrix jacchus)
No full textAdult neurogenesis in the primate brain is generally accepted to occur primarily in two specific areas; the subgranular zone (SGZ) of the hippocampal dentate gyrus (DG) and the subventricular zone (SVZ) of the lateral ventricles. Hippocampal neurogenesis is well known to be downregulated by stress and aging in rodents, however there is less evidence documenting the sensitivity of neuroblasts generated in the SVZ. In primates, migrating cells generated in the SVZ travel via a unique temporal stream (TS) to the amygdala and entorhinal cortex. Using adult common marmoset monkeys (Callithrix jacchus), we examined whether i) adult-generated cells in the marmoset amygdala differentiate into doublecortin-positive (DCX+) neuroblasts, and ii) whether lasting changes occur in DCX-expressing cells in the DG or amygdala when animals are exposed to 2 weeks of psychosocial stress. A surprisingly large population of DCX+ immature neurons was found in the amygdala of these 4-year-old monkeys with an average density of 163,000 DCX+ cells per mm(3). Co-labeling of these highly clustered cells with PSA-NCAM supports that a subpopulation of these cells are migratory and participate in chain-migration from the SVZ to the amygdala in middle-aged marmosets. Exposure to 2 weeks of isolation and social defeat stress failed to alter the numbers of BrdU+, or DCX+ cells in the hippocampus or amygdala when evaluated 2 weeks after psychosocial stress, indicating that the current stress paradigm has no long-term consequences on neurogenesis in this primate
What Values in Design? The Challenge of Incorporating Moral Values into Design
No full textRecently, there is increased attention to the integration of moral values into the conception, design, and development of emerging IT. The most reviewed approach for this purpose in ethics and technology so far is Value-Sensitive Design (VSD). This article considers VSD as the prime candidate for implementing normative considerations into design. Its methodology is considered from a conceptual, analytical, normative perspective. The focus here is on the suitability of VSD for integrating moral values into the design of technologies in a way that joins in with an analytical perspective on ethics of technology. Despite its promising character, it turns out that VSD falls short in several respects: (1) VSD does not have a clear methodology for identifying stakeholders, (2) the integration of empirical methods with conceptual research within the methodology of VSD is obscure, (3) VSD runs the risk of committing the naturalistic fallacy when using empirical knowledge for implementing values in design, (4) the concept of values, as well as their realization, is left undetermined and (5) VSD lacks a complimentary or explicit ethical theory for dealing with value trade-offs. For the normative evaluation of a technology, I claim that an explicit and justified ethical starting point or principle is required. Moreover, explicit attention should be given to the value aims and assumptions of a particular design. The criteria of adequacy for such an approach or methodology follow from the evaluation of VSD as the prime candidate for implementing moral values in design.Values and TechnologyTechnology, Policy and Managemen
The Thermodynamics of Economic Engineering: With Applications to Economic Growth
No full textThe economic engineering group at the DCSC uses Newtonian and analytical mechanics to model economic systems but makes no use of thermodynamics. The introduction of thermodynamics to the economic engineering framework would increase the extent of analysis and interpretation of economic systems in economic engineering. In this thesis the foundations of the thermodynamics of economic engineering are developed in order to include thermodynamic modeling of economic systems in the field of economic engineering. After the development of the thermodynamics of economic engineering theory, the theory is applied to analyze economic growth, factor productivity, and the value of a business. An axiomatic approach is taken to derive economic analogs to thermodynamic concepts. The meaning of an axiomatic approach is that these economic-thermodynamic analogs are developed in a logical order, e.g., the economic analog to temperature is not introduced before developing the economic analog to the first law. Key analogs between economics and thermodynamics are established in this thesis that include but are not limited to two fundamental economic laws, work as an expenditure, heat as an expense, temperature as a price level, and entropy as a quantity referred to as human capital in the thesis. By deriving key analogs, the thesis establishes the foundational principles of thermodynamics within economic engineering. Utilizing the theory of the thermodynamics of economic engineering results in applications to economic growth. Empirical growth accounting is modeled by the fundamental thermodynamic relationship. A relationship between linear production functions and Cobb-Douglas type production functions is shown by analyzing the productivity of an economy. Furthermore, a method to evaluate the worth of a business is created by determining the business' total potential earnings. Additionally, the thesis shares a vision of how to include thermodynamics within a control engineering framework. A higher-dimensional energy-based approach to model dynamical systems offers a way forward to include thermodynamic energy and entropy within control formalisms. Such a framework would account for availability and heat buildup in controlled dynamical systems. One potential application is to account for the heat build up that occurs in integrated circuits.This thesis is part of the Economic Engineering group at DCSC.Mechanical Engineering | Systems and Contro
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