301 research outputs found
Building a Genetics Curriculum: Needs Assessment Survey of Middle and High School Students
No full textMB002, a 5-page report by Jennifer C. Drew, Joy C. Jordan, and Eric W. Triplett, presents the results of a 1-page survey of 140 students at a 4-H sponsored event, which was designed to assist in developing a 4-H curriculum in genetics. Published by the UF Department of Microbiology and Cell Sciences, June 2006
Building a Genetics Curriculum: Needs Assessment Survey of Middle and High School Students
No full textMB002, a 5-page report by Jennifer C. Drew, Joy C. Jordan, and Eric W. Triplett, presents the results of a 1-page survey of 140 students at a 4-H sponsored event, which was designed to assist in developing a 4-H curriculum in genetics. Published by the UF Department of Microbiology and Cell Sciences, June 2006
Building a Genetics Curriculum: Needs Assessment Survey of Middle and High School Students
No full textMB002, a 5-page report by Jennifer C. Drew, Joy C. Jordan, and Eric W. Triplett, presents the results of a 1-page survey of 140 students at a 4-H sponsored event, which was designed to assist in developing a 4-H curriculum in genetics. Published by the UF Department of Microbiology and Cell Sciences, June 2006
How communications media are used by sixth and seventh grade pupils and their families, 1969
No full textComposição da comunidade bacteriana do solo sob sistemas integrados na região norte de Mato Grosso.
No full textOs sistemas integrados de produção agrícola estão sendo estudados como uma alternativa aos monocultivos tradicionais no intuíto de tornar a atividade agrícola sustentável, particulamente no estado do Mato Grosso. Entretanto, ainda não se conhece como essa atividade afeta as propriedades microbiológicas do solo. Assim, o objetivo deste trabalho foi avaliar e comparar os efeitos de sistemas integrados na composição da comunidade bacteriana do solo com diferentes monocultivos tendo como área referência uma mata nativa do bioma de transição Cerrado e Amazônia. O local de estudo está localizado na Embrapa Agrossilvipastoril no município de Sinop/MT e foram avaliados sete tratamentos (1-mata nativa, 2-lavoura, 3-pecuária, 4-eucalipto, 5-integração eucalipto lavoura (iEL), 6-integração eucalipto pecuária (iEP), 7-integração eucalipto lavoura pecuária (iELP)) dispostos em quatro blocos casualizados, os quais foram conduzidos segundo as recomendações agronômicas das espécies cultivadas. As amostras de solo foram coletadas nas épocas de chuva e estiagem em 2012 com o auxílio de trado holandês na profundidade de 0-10 cm, considerando 20 pontos aleatórios com caminhamento zigue-zague para fazer uma composta de cada tratamento. A extração de DNA total do solo foi realizada com o kit MoBio UltraClean? Soil DNA (MoBio Laboratories, Carlsbad, CA., EUA) conforme protocolo descrito pelo fabricante e sequenciado pela tecnologia Illumina (Illumina, Inc., CA, USA) com a plataforma MiSeq na Universidade da Florida (Gainesville, EUA). Por meio de ferramentas de bioinformática 2.957.127 sequencias do gene 16S rRNA com alta qualidade foram obtidas, sendo que 2.172.432 de OTUs (Operational Taxonomic Units) foram classificados para o domínio Bacteria, 3.564 Archaea e 262.994 não foram classificados. A composição bacteriana do solo com abundancia relativa ≥ 10 % a nível de filo apresentou três táxons, porém Firmicutes e Proteobacteria apresentaram padrão de comportamento de maior abundancia na estiagem em relação a chuva para mata nativa e iELP e para a lavoura o padrão foi o oposto . A nível de classe, dentre cinco filos apenas Actinobacteria variou o comportamento para iELP e mata nativa com menor abundância na estiagem e maior na chuva, tendo a lavoura o comportamento inverso e para Clostridia ocorreu o mesmo padrão porém ao contrário para os respectivos tratamentos. Analisando a diferença entre os tratamentos por meio da dissimiliradidade a nível de filo, corte de abundância relativa ≥ 0.05 % (18 filos na estiagem e 21 na chuva), o dendrograma baseado na distância euclidiana agrupou mata nativa e iELP como os mais semelhantes e lavoura o mais dissimilar dentre todos os tratamentos. Podemos concluir, que o sistema integrado eucalipto lavoura pecuária apresentou maior semelhança na sua composição bacteriana do solo com a da mata nativa em comparação com os demais tratamentos avaliados, como também manteve o padrão do comportamento da abundancia relativa parecido na época de estiagem, assim demonstrando indício de sustentabilidade
The methylome of the gut microbiome: disparate Dam methylation patterns in intestinal Bacteroides dorei
No full textDespite the large interest in the human microbiome in recent years, there are no reports of bacterial DNA methylation in the microbiome. Here metagenomic sequencing using the Pacific Biosciences platform allowed for rapid identification of bacterial GATC methylation status of a bacterial species in human stool samples. For this work, two stool samples were chosen that were dominated by a single species, Bacteroides dorei. Based on 16S rRNA analysis, this species represented over 45% of the bacteria present in these two samples. The B. dorei genome sequence from these samples was determined and the GATC methylation sites mapped. The Bacteroides dorei genome from one subject lacked any GATC methylation and lacked the DNA adenine methyltransferase genes. In contrast, B. dorei from another subject contained 20,551 methylated GATC sites. Of the 4,970 open reading frames identified in the GATC methylated B. dorei genome, 3,184 genes were methylated as well as 1,735 GATC methylations in intergenic regions. These results suggest that DNA methylation patterns are important to consider in multi-omic analyses of microbiome samples seeking to discover the diversity of bacterial functions and may differ between disease states
Tennessee Williams' treatment of women in his major plays, 1988
No full textThis thesis was undertaken to determine the Impact that the females In Tennessee Williams' family had on his portrayal of female characters n his major plays and to determine the extent that these characters were actually reflections of Williams himself. Williams' female characters fall Into three categories: the Southern genteel lady, female aggressor/mother-figure, and the survivors of corrupt societies. Williams' depiction of the women In each of these categories reflected his association with the females in his family. Rose, his sister, represented the fragile* sexually repressed Southern genteel lady. On the other hand, Williams' mother Edwina could be placed In all three categories of Williams' women since she possessed characteristics common to each category. She represented the fragile, genteel lady who lived 1n her own Illusions despite the drastic alteration of her life-style when her family made the move from Mississippi to Missouri. Like Amanda W1ngf1eld, Edwina was determined to keep her family going by any available means, and this characteristic was exemplary of a mother figure. Lastly, we may think of Edwina as having been a survivor who knew that life had to go on despite the fact that her daughter had to have a lobotomy and later was Institutionalized. The three categories of Williams1 women can also be said to be indicative of the phases of Williams1 homosexuality. The Southern genteel lady represented the early sexually repressed years of Williams' life. The female aggressor/mother-figure delineated his "coming out of the closet" or the public expression of his sexual preference despite criticism or ostracism. Lastly, the survivors in corrupt societies can be identified with Williams' final accomodation with the homosexual life-style
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