9 research outputs found
Effects of S6-Strain Mycoplasma gallisepticum Inoculation at 10, 22, or 45 Weeks of Age on the Digestive and Reproductive Organ Characteristics of Commercial Egg-Laying Hens
No full textEffects of S6-Strain Mycoplasma gallisepticum Inoculation at Ten, Twenty-Two, or Forty-Five Weeks of Age on the Egg Yolk Composition of Commercial Egg-Laying Hens
No full textNeurospora cells lacking the LSH/DDM1 homolog have normal DNA methylation but are hypersensitive to DNA damage.
No full text<p>(A) Southern hybridizations with probes corresponding to the methylated 8:A6 and 8:G3 regions were performed using genomic DNA from the indicated strains digested with the cytosine-methylation-sensitive BfuCI [B] and–insensitive DpnII [D] restriction enzymes. The gel stained with Ethidium Bromide is also shown (EtBr). Numbers on the left correspond to the DNA ladder (kb). (B) Box plots showing the average methylation level for all methylated regions in wildtype, plus Δ<i>ncu06306/crf5-1</i> strains from f1 and f2 generations. Two biological replicates are shown for each strain. The notches indicate the 95% confidence interval around the median. Overlapping notches indicate that the samples are not statistically different. (C) A metaplot showing the average distribution of DNA methylation across all methylated domains for wildtype and for Δ<i>ncu06306/crf5-1</i> strains from f1 and f2 generations. (D) Serial dilutions of conidia (10<sup>4</sup>−10<sup>1</sup>) of wildtype and <i>Δncu06306/crf5-1</i> were spotted on Vogel’s Minimal Medium (VMM) with or without the indicated genotoxic agents: methyl methanesulfonate (MMS; 0.025%), tert-Butyl hydroperoxide (TBP; 100 μM), camptothecin (CPT; 0.3 μg/mL), Bleomycin (BM; 0.2 μg/mL), Hydroxyurea (HU; 7mM). Cells were also exposed to Ultraviolet light (UV; 300 x 100mJ/cm2). (E) Introduction of wildtype <i>ncu06306/crf5-1+</i> complements the MMS-hypersensitive phenotype of <i>Δncu06306/crf5-1</i>. Serial dilutions of the indicated strains were spotted on minimal medium (VMM) with or without 0.025% MMS.</p
<i>Δmus-30</i> interacts genetically with <i>Δmag-1 and Δmei-3</i>.
No full text<p>(A) Serial dilutions of conidia (10<sup>4</sup>−10<sup>1</sup>) were spot tested on minimal medium (VMM) with or without the indicated concentrations of MMS for the indicated strains. (B) The average number of colonies for each genotype is shown for the indicated concentrations of MMS. For each concentration, % survival is shown relative to no MMS control. At least two isolates of each genotype were analyzed. Error bars show the standard deviation. (C) Repair of MMS-induced damage is shown in wildtype, <i>Δmus-30</i>, and <i>Δmag-1</i> cells, as indicated. Genomic DNA was isolated from cells before, during, and after MMS exposure, as indicated. DNA was treated with Human Alkyladenine DNA Glycosylase (hAAG), apurinic/apyrimidinic endonuclease (APE), or both to induce ssDNA breaks at methylated bases or abasic sites. The size of ssDNA was visualized at each time point by alkaline electrophoresis. A low molecular weight smear indicates the presence of unrepaired DNA after MMS treatment. (D) Images of race tubes containing minimal medium show the relative growth rates of the indicated strains. (E) The linear growth rate is plotted for multiple isolates of each genotype shown in D. (F) Serial dilutions of conidia (10<sup>4</sup>−10<sup>1</sup>) were spotted on minimal medium (VMM) with or without the indicated concentrations of MMS for wildtype and the indicated single mutants. For <i>Δmei-3</i>; <i>Δmus-30</i> strains, a dilution series from 10<sup>5</sup>−10<sup>2</sup> was used due to poor spore viability (asterisk).</p
Growing a circular economy with fungal biotechnology:a white paper
No full textFungi have the ability to transform organic materials into a rich and diverse set of useful products and provide distinct opportunities for tackling the urgent challenges before all humans. Fungal biotechnology can advance the transition from our petroleum-based economy into a bio-based circular economy and has the ability to sustainably produce resilient sources of food, feed, chemicals, fuels, textiles, and materials for construction, automotive and transportation industries, for furniture and beyond. Fungal biotechnology offers solutions for securing, stabilizing and enhancing the food supply for a growing human population, while simultaneously lowering greenhouse gas emissions. Fungal biotechnology has, thus, the potential to make a significant contribution to climate change mitigation and meeting the United Nation's sustainable development goals through the rational improvement of new and established fungal cell factories. The White Paper presented here is the result of the 2nd Think Tank meeting held by the EUROFUNG consortium in Berlin in October 2019. This paper highlights discussions on current opportunities and research challenges in fungal biotechnology and aims to inform scientists, educators, the general public, industrial stakeholders and policymakers about the current fungal biotech revolution.</p
Adaptación de la escala de bienestar psicológico de Ryff con una muestra de sobrevivientes del conflicto armado colombiano
No full textAbstract Measurement with context-standardized tools represents a quality factor for evidence-based assessment. The Ryff Psychological Well-Being scale and its adaptations in the Hispanic population lack evidence of validity for the surviving population of the armed conflict. The present study analyzes the psychometric properties and estimates the cut-off point, sensitivity and specificity of the Ryff Psychological Well-being scale. An instrumental quantitative study was conducted with 297 survivors between 18 and 78 years ( =41.83 S=14.76) and 200 participants from the general population between 18 and 69 years ( =32.68 S=12.20). A scale of 22 items resulting from psychometric analyses is proposed. The confirmatory factor analysis validates the theoretical proposal of the six dimensions proposed by the authors. The Cronbach alpha and McDonald’s ω coefficients were .69 and .70. The set cut-off point was 89.5, with a sensitivity of .92 and specificity of .62. The findings open a new path of exploration in the field of study given that there are no validation investigations that compare groups with particular characteristics in Colombia.Resumo A medição com ferramentas padronizadas pelo contexto representa um fator de qualidade para a avaliação baseada em evidências. A escala de bem-estar psicológico de Ryff e suas adaptações na população hispânica carecem de evidências de validade para a população sobrevivente do conflito armado. O presente estudo analisa as propriedades psicométricas e estima o ponto de corte, a sensibilidade e a especificidade da escala de Bem-estar Psicológico de Ryff. Um estudo quantitativo instrumental foi realizado com 297 sobreviventes entre 18 e 78 anos ( =41,83 S=14,76) e 200 participantes da população geral entre 18 e 69 anos ( =32,68 S=12,20). Uma escala de 22 itens é proposta como resultado das análises psicométricas. A análise fatorial confirmatória valida a proposta teórica das seis dimensões propostas pelos autores. Os coeficientes alfa de Cronbach e ω McDonald’s foram de 0,69 e 0,70. O ponto de corte estabelecido foi de 89,5, com sensibilidade de 0,92 e especificidade de 0,62. Os resultados abrem um novo caminho de exploração no campo de estudo, uma vez que não existem investigações de validação que comparem grupos com características particulares na Colômbia.Resumen La medición con herramientas estandarizadas al contexto representa un factor de calidad para la evaluación basada en la evidencia. La escala de bienestar psicológico de Ryff y sus adaptaciones en población hispana carecen de evidencia de validez para población sobreviviente del conflicto armado. El presente estudio analiza las propiedades psicométricas y estima el punto de corte, sensibilidad y especificidad de la escala de bienestar psicológico de Ryff. Se efectuó un estudio cuantitativo instrumental con 297 sobrevivientes entre 18 y 78 años (= 41,83 S = 14,76) y 200 participantes de población general entre 18 y 69 años ( = 32,68 S = 12,20). Se propone una escala de 22 ítems resultado de los análisis psicométricos. El análisis factorial confirmatorio valida la propuesta teórica de las seis dimensiones planteadas por los autores. Los coeficientes alfa de Cronbach y ω de McDonald’s fueron ,69 y ,70. El punto de corte establecido fue 89,5, con una sensibilidad de ,92 y especificidad de ,62. Los hallazgos abren un nuevo camino de exploración en el campo de estudio dado que no se registran investigaciones de validación que comparen grupos con características particulares en Colombia
Formulación del plan de gestión integral de residuos sólidos generados en la empresa de textiles Dyetex S.A.S en la ciudad de Bogotá D.C.
No full textEl Plan Integral de Residuos Sólidos para una empresa textil tiene como objetivo principal la reducción de los residuos generados por la empresa, así como su correcta gestión y disposición final. En este caso la mala disposición de los residuos trae consigo problemas a nivel social ambiental y económico, dentro de los procesos productivos muchas empresas implementan sistemas de gestión en los que se puedan identificar los tipos de residuos y así mismo como se pueden tratar, dentro del concepto de industria textil es una de las actividades industriales que mas contamina, es por esto que la implementación del PGIRS desarrollara un cambio en la mentalidad de la economía global, dentro de sus políticas de posconsumo, como los programas que se logran desarrollar con el fin de disminuir la cantidad de residuos solidos y generar una adecuada disposición de los mismos.The Comprehensive Solid Waste Plan for a textile company has as its main objective the reduction of waste generated by the company, as well as its correct management and final disposal. In this case, the poor disposal of waste brings with it social, environmental and economic problems. Within the production processes, many companies implement management systems in which the types of waste can be identified and also how they can be treated, within the concept of the textile industry is one of the industrial activities that pollutes the most, which is why the implementation of the PGIRS will develop a change in the mentality of the global economy, within its post-consumer policies, such as the programs that are developed in order to to reduce the amount of solid waste and generate an adequate disposal of the same.1. PLANTEAMIENTO DEL PROBLEMA -- 2. JUSTIFICACION -- 2.1 Justificación teórico -- 2.2 Justificación practica -- 2.3 Justificación metodológica -- 3. OBJETIVOS -- 3.1. Objetivo general -- 3.2. Objetivos específico. -- 4. DELIMITACIÓN -- 4.1. Delimitación temática -- 4.2. Delimitación espacial -- 4.3. Delimitación temporal -- 5. MARCO TEORICO -- 5.1. Antecedentes -- 6. MARCO CONCEPTUAL -- 6.1. PGIRS “plan integral de residuos sólidos” -- 6.1.1. Elaboración del PGIRS -- 6.1.2. Gestión integral de residuos sólidos. -- 6.1.3. Residuo sólido o desecho -- 6.1.4. Residuo sólido aprovechable -- 6.1.5. Reutilización -- 6.1.6. Reciclaje -- 6.1.7. Recolección -- 6.1.8. Recuperación -- 6.1.9. Relleno sanitario -- 6.1.10. Disposición final de residuos -- 6.1.11 Disposición final y origen de residuos solidos -- 7. MARCO LEGAL -- 8. MARCO INSTITUCIONAL -- 8.1. Misión -- 8.2 Visión -- 8.3 Política y principio de la empresa -- 8.4 Objetivos8.5 Línea de servicios -- 8.6 Estructura organizacional -- 9. METODOLOGIA -- 9.1. Tipo de investigación -- 9.2 DIAGNOSTICO -- 9.2.1. Fase I. Identificación del manejo interno de los residuos. -- 9.3 Fase II. Matriz Dofa -- 9.3.1 Matriz DOFA, identificación de fortalezas, debilidades, oportunidades y amenazas. -- 9.4. MATRIZ DE IMPACTOS -- 9.4.1. Impactos que recaen sobre la mala disposición de los residuos. -- 9.5. FORMULACIÓN Y ESTRUCTURACIÓN DEL PGIRS -- 9.5.1. Planteamiento del PGIRS, en la compañía. -- 9.5.2. Capacitaciones según resultados obtenidos. -- 10. RESULTADOS -- 10.1 DIAGNOSTICO -- 10.1.1 FASE l: Identificación de residuos -- 10.1.2 Residuos generados en las diferentes áreas de la empresa Dyetex SAS. -- 10.2 FASE II. Caracterización de los residuos -- 10.2.1 Caracterización método de cuarteo -- 10.3 FASE III. Implementación de matrices -- 10.3.1 Matriz DOFA -- 10.3.1 Matriz Leopold -- 11. RECURSOS Y PRESUPUESTO -- 12. CRONOGRAMA-- [email protected]
Aplicación de la economía circular en el proceso de cultivo de macrohongos en subproductos del café
No full textFiguras, tablasLos macrohongos contienen enzimas hidrolíticas (celulosas y hemicelulosas) y oxidativas (lacasas y peroxidosas) que permiten degradar y acelerar los procesos de descomposición de la materia orgánica, como los residuos de origen agrícola, contribuyendo al ciclo del carbono. Además, los macrohongos forman estructuras reproductivas visibles que cumplen funciones claves en el ciclo de vida del hongo y que, a su vez, pueden tener un valor biotecnológico importante para la sociedad. Por lo tanto, la comprensión del ciclo de vida y los requerimientos ambientales de los macrohongos pueden contribuir a la descomposición de residuos agrícolas como los subproductos del cultivo del café (Coffea arabica) y a la producción de biomasa con potencial medicinal, nutricional o biorremediador. En este sentido, en esta investigación exploratoria, se buscó evaluar la viabilidad del cultivo de tres especies de macrohongos: Ganoderma lucidum, Pleurotus ostreatus y Coprinus sp, en subproductos del café, principalmente del pericarpio del fruto (pulpa) que es el principal subproducto del beneficio del café. Para ello, se buscó evaluar las condiciones ambientales de humedad, pH, luminosidad y temperatura óptimos para cada una de las fases del cultivo de los hongos mencionados bajo una lógica de economía circular sustentable. Los resultados mostraron que la adecuación del sustrato óptima a base de pulpa para G. lucidum fue con fermentación sumergida de 10 días con peróxido de hidrógeno al 1 % y esterilización con autoclave por 20 minutos con el cual se obtuvo una eficiencia biológica de 5,8 %. Por otro lado, se encontró que es posible el crecimiento de P. ostreatus en residuos del cultivo de G. lucidum con tiempos más prolongados de crecimiento. Además, no se observó colonización completa de Coprinus sp. en pulpa de café fermentada 10 días o lavada, lo cual podría indicar que se necesita períodos más extensos de degradación de la pulpa para su crecimiento. En conclusión, G. lucidum es una especie que tiene un crecimiento potencial en la pulpa del café, lo cual puede aportar significativamente a la valorización de los residuos del café y a la producción de setas medicinales a través de un sistema de economía circular sustentable.Macrofungi contain hydrolytic (cellulose and hemicellulose) and oxidative (laccase and peroxidases) enzymes that degrade and accelerate the decomposition of organic matter, such as agricultural waste, contributing to the carbon cycle. Furthermore, macrofungi form visible reproductive structures that play key roles in the fungal life cycle and, in turn, can have significant biotechnological value for society. Therefore, understanding the life cycle and environmental requirements of macrofungi can contribute to the decomposition of agricultural waste, such as coffee (Coffea arabica) by-products, and to the production of biomass with medicinal, nutritional, or bioremediation potential. In this exploratory study, we look into to evaluate the viability of cultivating three species of macrofungi: Ganoderma lucidum, Pleurotus ostreatus, and Coprinus sp., in coffee by-products, primarily the fruit pericarp (pulp), which is the main by-product of the coffee processing industry. To this end, we look into to evaluate the optimal environmental conditions of humidity, pH, light, and temperature for each of the cultivation phases of the aforementioned fungi with a sustainable circular economy framework. The results showed that the optimal pulp-based substrate for G. lucidum was a 10-day submerged fermentation with 1% hydrogen peroxide and 20-minute autoclave sterilization, obtaining a biological efficiency of 5.8%. Furthermore, we found that P. ostreatus can grow on G. lucidum crop residues with longer growth times. Furthermore, complete colonization of Coprinus sp. was not observed in fermented or washed coffee pulp for 10 days, which could indicate that longer pulp degradation periods are required for its growth. In conclusion, G. lucidum is a specie with potential for growth in coffee pulp, which can significantly contribute to the valorization of coffee waste and the production of medicinal mushrooms through a sustainable circular economy system.Introducción -- Planteamiento del problema -- Justificación -- Marco teórico y antecedentes -- Cultivo de Ganoderma lucidum -- Sustratos -- Rendimientos del proceso -- Condiciones ambientales de operación -- Factores nutricionales -- Costos -- Cultivo de Pleurotus ostreatus -- Condiciones del sustrato -- Condiciones ambientales -- Cultivo de Coprinus sp -- Condiciones ambientales -- Materiales y métodos -- Aislamiento de las cepas -- Activación de las cepas -- Producción de inóculo -- Elaboración del sustrato -- Incubación -- Fructificación -- Cosecha -- Resultados y discusión -- Aislamiento y activación de cepas -- Producción de inóculo e incubación -- Fructificación y cosecha -- Selectividad biológica de macrohongos -- Conclusiones -- Referencias.PregradoBiólogo(a)Universidad de CaldasMicologí
VEuPathDB: the eukaryotic pathogen, vector and host bioinformatics resource center
No full textThe Eukaryotic Pathogen, Vector and Host Informatics Resource (VEuPathDB, https://veupathdb.org) represents the 2019 merger of VectorBase with the EuPathDB projects. As a Bioinformatics Resource Center funded by the National Institutes of Health, with additional support from the Welllcome Trust, VEuPathDB supports >500 organisms comprising invertebrate vectors, eukaryotic pathogens (protists and fungi) and relevant free-living or non-pathogenic species or hosts. Designed to empower researchers with access to Omics data and bioinformatic analyses, VEuPathDB projects integrate >1700 pre-analysed datasets (and associated metadata) with advanced search capabilities, visualizations, and analysis tools in a graphic interface. Diverse data types are analysed with standardized workflows including an in-house OrthoMCL algorithm for predicting orthology. Comparisons are easily made across datasets, data types and organisms in this unique data mining platform. A new site-wide search facilitates access for both experienced and novice users. Upgraded infrastructure and workflows support numerous updates to the web interface, tools, searches and strategies, and Galaxy workspace where users can privately analyse their own data. Forthcoming upgrades include cloud-ready application architecture, expanded support for the Galaxy workspace, tools for interrogating host-pathogen interactions, and improved interactions with affiliated databases (ClinEpiDB, MicrobiomeDB) and other scientific resources, and increased interoperability with the Bacterial & Viral BRC
