24,400 research outputs found
The M, E, and N structural proteins of the severe acute respiratory syndrome coronavirus are required for efficient assembly, trafficking, and release of virus-like particles
Get PDFCopyright @ 2008 American Society for Microbiology.The production of virus-like particles (VLPs) constitutes a relevant and safe model to study molecular determinants of virion egress. The minimal requirement for the assembly of VLPs for the coronavirus responsible for severe acute respiratory syndrome in humans (SARS-CoV) is still controversial. Recent studies have shown that SARS-CoV VLP formation depends on either M and E proteins or M and N proteins. Here we show that both E and N proteins must be coexpressed with M protein for the efficient production and release of VLPs by transfected Vero E6 cells. This suggests that the mechanism of SARS-CoV assembly differs from that of other studied coronaviruses, which only require M and E proteins for VLP formation. When coexpressed, the native envelope trimeric S glycoprotein is incorporated onto VLPs. Interestingly, when a fluorescent protein tag is added to the C-terminal end of N or S protein, but not M protein, the chimeric viral proteins can be assembled within VLPs and allow visualization of VLP production and trafficking in living cells by state-of-the-art imaging technologies. Fluorescent VLPs will be used further to investigate the role of cellular machineries during SARS-CoV egress.The University of Hong Kong and the French Ministry of Health
Hybrid Numerical Analysis of a high-speed non-volatile Suspended Gate Silicon Nanodot Memory
No full textWe present a hybrid numerical analysis of a high-speed and non-volatile suspended gate silicon nanodot memory (SGSNM) which co-integrates a nano-electromechanical (NEM) control gate with a MOSFET as a readout element and silicon nanodots as a floating gate. A hybrid NEM-MOS circuit simulation is developed by taking account of the pull-in/pull-out operation of the suspended gate and electron tunnelling processes through the tunnel oxide layer as behavioural models. The signals for programming, erasing and reading are successfully achieved at circuit level simulation. The programming and erasing times are found as short as 2.5 nsec for a SGSNM with a 1-?m-long suspended gate, which is a summation of the mechanical pull-in/pull-out times and the tunnel charging/discharging times
Variable structure model for flow-induced tonal noise control with plasma actuators
No full textThe objective of this work was to study the effect of plasma actuators in attenuating low-speed flow-induced cavitytones from a control point of view by employing techniques from classical control. A modification of the existingphysics-based linear model produced a new variable structure model in which a plasma actuator was regarded as alinear gain. The parameters of the overall model working at two operating voltages were identified usingexperimental data. The effects of the plasma actuator control at other various operating voltages were thus able to bepredicted using linear interpolation. The good agreement between the predicted and the measured data supportedthe proposed variable structure model, inside of which plasma actuators affected the damping of cavity pressureoscillations proportionally to the applied voltage to reduce flow-induced tonal noise. With the proposed variablestructure model the system stability controlled by plasma actuators at various operating voltages was ensured, thus aclosed-loop control method could be applied without leading to instability. A simple proportional integral derivativecontroller was implemented. Results show the potential of a closed-loop method by increasing system powerefficiency
Data for: Conserving unprotected important coastal habitats in the Yellow Sea: shorebird occurrence, distribution and food resources at Lianyungang
No full textThis shapefile contains reclamation occurred along the Lianyungang Coast (34.5-35.2°N, 119.1-119.7°E) from January 2003 to June 2018, mapped from Landsat and Sentinel satellite images of 30 m resolution. Land use of the reclaimed areas (as of June 2018) were classified into aquaculture ponds, industrial land or undeveloped land
Uniqueness of entire ground states for the fractional plasma problem
Get PDFWe establish uniqueness of vanishing radially decreasing entire solutions, which we call ground states, to some semilinear fractional elliptic equations. In particular, we treat the fractional plasma equation and the supercritical power nonlinearity. As an application, we deduce uniqueness of radial steady states for nonlocal aggregation-diffusion equations of Keller-Segel type, even in the regime that is dominated by aggregation
Efectos de la Conquista española sobre la cerámica prehispánica de Cholula.. Anales del Instituto Nacional de Antropología e Historia. Num. 51 Tomo III (1970-1971) Séptima Época (1967-1976)
No full textBruggemann, Jürgen K. 1969 El Sur del Centro de Veracruz: Un área en transición. Tesis profesional, ENAH, México.Códice Borgia. 1963 México-Buenos Aires. Fondo de Cultura Económica, 2 vol.Coe, M D. 1965 Archaeological Synthesis of Southern Veracruz, and Tabasco. Handbook of Middle American Indians, Part 11, Vol 3. Austin. University of Texas Press.Charlton, Thomas H. 1968 Post-Conquest Ceramics. Tallahasse, Florida. The Florida Anthropological Society, p 96-101.Delgado, Agustín. 1965 Investigaciones arqueológicas en las Cuevas del Sur de Tehuacán, Pue., y Sur de Córdoba, Ver. Anales del INAH. Tomo XVII, p 75-107.Drucker, Philip. 1943 a Ceramic Secuences at Tres Zapotes, Veracruz, México. Washington: Smith. Inst. Bur. Am. Ethnol. Bull. 140.Drucker, Philip. 1943 b Ceramic Stratigraphy at Cerro de las Mesas, Veracruz, México. Washington: Smith. Inst. Bur. Am. Ethnol., Bull 141.Drucker, Philip. 1955 The Cerro de las Mesas Offering Jade and Other Materials. Washington: Smith. Inst. Bur. Am. Ethnol., Bull. 157.Dusolier, Wilfrido. 1939 Principales conclusiones obtenidas del estudio de la cerámica arqueológica de El Tajín. Actas del XXVII Congreso Internacional de Americanistas, tomo II, 147-192.García Payón, José. 1951 La cerámica de fondo 'sellado' de Zempoala, Veracruz. Homenaje a Alfonso Caso, pp 2; 189-198. México. INAH.Mac Neish, Richard, F A Peterson, y K V Flannery. 1970 Ceramics: The Prehistory of the Tehuacan Valley, Vol III. University of Texas Press Austin - London.Marquina, Ignacio. 1970 Cuadro cronológico de Cholula. México, D F. Proyecto Cholula. Informe INAH.Goggin, John M. 1968 Spanish Mayolica in the New World. New Haven, Conn. Yale University, Dept of Anthropology, No. 72.Medellín Zenil, Alfonso. 1952 Exploraciones en Cuauhtochco, Xalapa, Ver.Medellín Zenil, Alfonso. 1955 Exploraciones en la Isla de Sacrificios, Veracruz.Medellín Zenil, Alfonso. 1960 Cerámicas del Totonacapan. Xalapa: Universidad de Veracruz.Munsell. 1954 Soil Color Charts. Baltimore: Munsell Color Company, Inc.Noguera, Eduardo. 1954 La Cerámica de Cholula. México: Editorial Guaranía.Noguera, Eduardo. 1965 La Cerámica Arqueológica de Mesoamérica. México: UNAM. lnst. de Invest. Hist.Piña Chan, Román. 1960 Mesoamérica. México: INAH.Piña Chan, Román. 1967 a Una visión del México Prehispánico. México: UNAM.Piña Chan, Román. 1967 b Un complejo Coyotlatelco en Coyoacán, D. F. Anales de Antropología, Vol IV, UNAM México. México: INAH, p 141-160.Piña Chan, Román. 1968 Jaina. México: INAH.Shepard, A O. 1956 Ceramics for the Archaeologist. Washington: Carnegie Institution.Tolstoy, Paul. 1958 Surface Survey of the Northern Valley of Mexico: The Classic and Postclassic Periods. Philadelphia: Am. Phil. Soc. Transactions núm. 48.Mapas. Mapa de provincias climatológicas basado en el sistema de clasificación de Thornthwaite. Instituto de Geografía, México
Autoantibodies to Argonaute 2 (Su antigen)
No full textLike many other classical autoantibodies in systemic rheumatic diseases, anti-Su antibodies were originally defined by the double immunodiffusion assay in the early 80s. However, despite its high prevalence, only a few reports on anti-Su were published in the following years and the progress in characterizing the target antigens and clinical significance was slow, probably due to its inconsistent or poor reactivity in other standard immunoassays. In 2006 the target antigen was identified as the microRNA (miRNA)-binding protein Argonaute 2 (Ago2). Ago2 is a key component of the RNA-induced silencing complex enriched in cytoplasmic foci called GW bodies. Due to preferential reactivity of human autoantibodies with native antigens, immunoprecipitation is the only method to reliably detect anti-Su/Ago2 antibodies. Anti-Su/Ago2 does not appear to have disease specificity since it is found in 10-20% of patients with various rheumatic diseases, including systemic lupus erythematosus, scleroderma, polymyositis/dermatomyositis, and Sjögren's syndrome, as well as apparently healthy individuals at lower prevalence. The clinical significance and the mechanism of production of anti-Su/Ago2 remains to be clarified
Pachydiadematidae Chan & Dreyer & Gale & Glenner & Ewers-Saucedo & Pérez-Losada & Kolbasov & Crandall & Høeg 2021, FAM. NOV.
No full text††FAMILY PACHYDIADEMATIDAE FAM. NOV. Z o o b a n k r e g i s t r a t i o n: l s i d: u r n: l s i d: z o o b a n k. org:act: A16DF979-F1B2-4678-87F3-D3753B05F90B † † Pa ch y d i a d e m a Wi t h e r s, 1 9 3 5 (T y p e g e n u s) (Cretaceous) (one species)Published as part of Chan, Benny K. K., Dreyer, Niklas, Gale, Andy S., Glenner, Henrik, Ewers-Saucedo, Christine, Pérez-Losada, Marcos, Kolbasov, Gregory A., Crandall, Keith A. & Høeg, Jens T., 2021, The evolutionary diversity of barnacles, with an updated classification of fossil and living forms, pp. 789-846 in Zoological Journal of the Linnean Society 193 on page 833, DOI: 10.1093/zoolinnean/zlaa160, http://zenodo.org/record/563727
Bibliographics for the 983 eprints in the live archives of E-LIS : trends and status report up to 7th July 2004, based on author-self-archiving metadata
Get PDFThe priority for ideas and philosophy related to "Network Theory" have been traced back and documented by Braun(2004),and credit goes to Karinthy(1929).The IT has empowered to realise it, as the most practical phenomena and it is no more a humour. The OAI (Open Archives Initiatives)and ACIS (Academic Contributor Information System)are progressive in the direction ,which may lead to realise the "Collective Genius" at global level. Focus of present study is on Author-Self-Archiving (A-S-A)Metadata of the 983 Eprints in the Live Archives of the E-LIS (EPrints of Library and Information Science),which were approved till 7th July 2004.The A-S-A Metadata was used for librametric analysis. Self-explanatory bibliographics are illustrated.The highlights include: Conference papers (34%); highest approval, June 2004 (28%); published archives (76%);not refereed (52%); not in public domain (60%); highest self-archiving-author (De Robbio, Antonella).The Nos. of EPrints having single JITA domain specifications were: Theoretical and general aspects of libraries and information(27); Information use and sociology of information(80);Users,literacy and reading(13);Libraries as physical collections(30);Publishing and legal issues(57);Management(13);Industry, profession and education(36);Information sources, supports, channels(113) ; Information treatment for information services, Information functions and techniques (101); Technical services libraries, archives and museums(25); Housing technologies(1); Information technology and library technology(92); and Inter-domainery (395) i.e. having specifications of two or more than two JITA classes
A Pre-filter Enabling Fast Frontal Face Detection
No full textWe present a novel pre-filtering technique that identifies probable frontal illuminated face regions in colour images regardless of translation, orientation, and scale. The face candidate regions are normalised and provide the basis for face verification using published face detection algorithms. The technique focuses on a fast search strategy to locate potential eye-pairs in an image or video frame. The eye-pair candidates indicate areas that may contain faces. Scale and orientation is inferred from the eye-pairs, and a neural network is used to confirm the normalised face candidates
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