394 research outputs found
Tick-borne encephalitis virus and the immune response of the mammalian host
No full textTick-borne encephalitis (TBE) is caused by Tick-borne encephalitis virus (TBEV), one of the most prevalent arboviruses in Europe and in many parts of Asia. Transmission of TBEV to humans usually occurs by bite of an infected tick or rarely by ingestion of unpasteurized milk products of infected livestock. TBEV infection induces an innate and adaptive immune response, nevertheless it is able to replicate in several cell types of the immune system at the same time which probably contributes to the spread of the virus in the human host. Furthermore, TBEV can enter the central nervous system (CNS) by yet not well understood mechanisms via the blood brain barrier (BBB) or the olfactory neurons which leads to serious neurological disorders like meningitis, encephalitis or even meningoencephalitis. In this article we review the known facts and possible hypotheses of interaction of TBEV with components of the mammalian immune system and their implications for TBEV-mediated pathogenesis. (C) 2010 Elsevier Ltd. All rights reserved.German federal ministry of education and research (BMBF) [01KI0710
SPINE: A method for the rapid detection and analysis of protein-protein interactions in vivo
No full textThe detection and analysis of protein-protein interactions is one of the central tasks of proteomics in the postgenomic era. For this purpose, we present a procedure, the Strep-protein interaction experiment (SPINE) that combines the advantages of the Strep-tag protein purification system with those of reversible in vivo protein crosslinking by formaldehyde. Using two Bacillus subtilis regulator proteins, we demonstrate that this method is well suited to isolate protein complexes with high purity and virtually no background. Plasmids allowing the high-level expression of proteins carrying an N- or C-terminal Strep-tag in B. subtilis were constructed
Isolation and molecular characterization of a tick-borne encephalitis virus strain from a new tick-borne encephalitis focus with severe cases in Bavaria, Germany
No full textTick-borne encephalitis (TBE) is the most important viral infection transmitted by ticks in Central Europe In Germany where TBE was classified as a notifiable disease in 2001 a highly variable number of clinically apparent human cases was reported in the last few years ranging from the lowest number of 238 in 2007 to a maximum of 546 in 2006 The dynamics of the virus and its vector tick remain poorly understood We investigated a highly active TBE focus in south-eastern Germany where from 2003 to 2008 a total of 9 clinical human cases was diagnosed Three out of these 9 cases were fatal indicating an unusually high mortality rate possibly due to a highly virulent TBEV strain From 2005 till 2008 2150 Ixodes ricinus ticks were collected and tested for the presence of TBE virus Five TBEV-positive ticks were detected by real-time RT-PCR A viable virus strain was isolated from one of the positive ticks sampled in 2005 This is the first TBE virus isolate from a tick in Germany for 30 years Sequencing of the full-length genome of this virus strain (AS 33) revealed 2 unique amino acid substitutions in the envelope protein known to play a role in the pathogenicity of TBE virus Amplification of the envelope gene using 2 TBEV-PCR-positive ticks from 2006 also showed these particular mutations indicating that this TBE virus strain was present in at least 2 consecutive years The entire sampling area was divided into smaller sectors for the exact location of TBEV-positive ticks Virus-positive ticks were found to be randomly distributed throughout the investigated focus which is used as recreational area by the local people (C) 2009 Elsevier GmbH All rights reserve
Aufbau und Test eines Detektorsystems zum Koinzidenznachweis von Ionen und Photonen für die kollineare Laserspektroskopie am TRIGA-Forschungsreaktor [30.06.2010]
No full textOnTEAM metadata: GDSID: DOC-2010-Oct-71; Attribute ID: LIBRARY-thesis_diplom-2010-005; Title: [GSI Dipl 2010-03] Aufbau und Test eines Detektorsystems zum Koinzidenznachweis von Ionen und Photonen für die kollineare Laserspektroskopie am TRIGA-Forschungsreaktor [30.06.2010]; Author(s): Sieber, Bastian; Corporate author(s): ; Publication date: 20101014; Creator: manton; Creation date: 14.10.2010 15:32:56; Change date: 31.01.2011 17:45:03; Access: Welt; Attribute type: Thesis.Diplom; Directory path: ['GSI Publications', 'GSI as Publisher']; Attribute path: ['Infrastructure', 'Library and Documentation', 'thesis_diplom', 'Added in 2010']; File name(s): ['DOC-2010-Oct-71-1.pdf']; File title(s): ['']; File access: ['GSI-intern'
True Style: The History & Principles of Classic Menswear
No full textG. Bruce Boyer, men’s fashion editor and author of True Style: The History & Principles of Classic Menswear, discussed contemporary men’s dress and its history, styles, principles, and trends. He was joined by Pulitzer Prize- winning journalist Jonathan Capehart; New York Times cultural reporter Guy Trebay, and Michael Bastian; and MFIT Deputy Director Patricia Mears
Monitoring biodegradation capacity of organic pollutants in the environment
No full textMicro-organismen zijn in staat om organische verbindingen om te zetten in minder schadelijke stoffen en spelen daarom een belangrijke rol bij het opruimen van milieuvervuiling. Voor beleidsmakers, landgebruikers en landeigenaren is het belangrijk dat er bij milieuverontreiniging goed toezicht wordt gehouden op de biologische afbraakprocessen en dat deze goed worden beheerst. Aangezien microbiële activiteit in het milieu wordt beïnvloed door diverse fysische, geochemische en biologische factoren, is nauwkeurige kennis van het afbraakproces hierbij noodzakelijk. In dit promotieonderzoek wordt de relatie tussen geochemische condities en de biologische afbraakcapaciteit van micro-organismen in het milieu beschreven, inclusief methoden om de activiteit en metabole functies van deze micro-organismen in het milieu te mete
A study of "The Neverending Story" as an allegory of creative writing: The figures of reader and writer in Bastian
No full textEn este trabajo se realiza un análisis semántico-ficcional de La historia interminable de Michael Ende (1979). La hipótesis de partida supone considerar la obra como una alegoría del proceso de escritura creativa, que engloba la lectura previa y el propio proceso de escritura. Se estudia este simbolismo en el protagonista, Bastián, que encarna las figuras del lector y el autor tanto de forma literal en el texto como de forma alegóricaIn this work I perform a semantic-fictional analysis of The Neverending Story, by Michael Ende (1979). My starting hypothesis is to consider the novel as an allegory of the creative writing process. I study this symbolism in the principal character, Bastian, who incarnate the figures of reader and author in the text, literally and alegoricall
Author Biographies
No full textPhilipp-Bastian Brutscher is Senior Economist in the Economics Department of the European Investment Bank (EIB) where he is principally responsible for the EIB Investment Survey, a large-scale survey of corporate investment activities. Philipp acts as the focal point for the Department’s analytical work on business and infrastructure investment activities. He holds a Master’s and PhD degree from the University of Cambridge. Rocco Luigi Bubbico is Policy Advisor in the EIB Permanent R..
Sport as an integration tool for unaccompanied minor refugees : a project in Fürstenfeld
No full textDie enormen Flüchtlingsströme in Europa haben die Politik und die Gesellschaft veranlasst sich vermehrt mit dem Thema der Integration und erfolgreicher Integrationsmaßnahmen zu beschäftigen. Diese Arbeit befasst sich mit der Frage, inwieweit Sport einen Beitrag zur Integration leisten kann. Dabei wird der Fokus auf die Zielgruppe der unbegleiteten minderjährigen Flüchtlinge gesetzt. Auf theoretischer Ebene werden einleitend die sozialen sowie politischen Aspekte hinsichtlich Flucht und Asyl bearbeitet, unter anderem werden unterschiedliche Fluchtmotive, das Asylverfahren in Österreich und aktuelle Integrationsmaßnahmen behandelt. Ebenso werden verschiedene theoretische Integrationskonzepte sowie die Potenziale von Sport als Mittel der Integration aufgezeigt. Die theoretischen Ansätze und Erkenntnisse fließen in weiterer Folge in ein vom Autor initiiertes Sportprojekt für unbegleitete minderjährige Flüchtlinge ein. Dabei werden das Konzept des Projekts, die Organisationsmaßnahmen, die Durchführung sowie die Erkenntnisse und Erfahrungen beschrieben und reflektiert.The enormous streams of refugees in Europe caused politics and the society to deal increasingly with the reoccurring topic of integration and successful integration measures. This thesis deals with the question in what way sports can contribute towards integration. The focus is on the target group of unaccompanied minor refugees. In the beginning the social and political aspects regarding the flight and the asylum are worked on, there will be mentioning of different motives for refugees to leave their country, the process of applying for Asylum in Austria and current measures for integrating refugees amongst others. Also the thesis will reveal different theoretic integration concepts as well as the potential of sports to be used as an integration tool. The theoretical approaches and findings are then incorporated into a sports project initiated by the author for unaccompanied minor refugees. With doing so the concept of the project, the measures for organizing it, the execution as well as the knowledge and experiences are described.vorgelegt von Bastian JedliczkaAbweichender Titel laut Übersetzung des Verfassers/der VerfasserinZusammenfassungen in Deutsch und EnglischDiplomarbeit Karl-Franzens-Universität Graz 2018 2.03
Capacity adjustment factors for connected and automated vehicles in the Highway capacity manual: phase 1 and 2 final report : pooled fund study led by Oregon DOT
No full textThis project's objective was to develop capacity adjustment factors (CAFs) for connected and automated vehicles (CAVs) at different levels of market penetration to allow the HCM to be used to analyze CAV applications on freeways and urban streets. The primary approach to this problem was through an "agent-based" (i.e., fully customizable vehicle and driver behavior) simulation modeling framework in which CAV and non-CAV behavior could be modeled differently. The products of this research are highway capacity adjustment lookup tables and figures for different transportation system elements (e.g., freeways, roundabouts, signalized intersections) at different levels of CAV market penetration, and example scenarios demonstrating the application of the CAFs to planning studies.Report -- Appendix A.by Bastian Schroeder, PhD, PE, Abby Morgan, PhD, PE, Paul Ryus, PE, Burak Cesme, PhD, Apoorba Bibeka, Lee Rodegerdts, PE, and Jiaqi Ma, PhD ; for Oregon Department of Transportation, Research Section and Federal Highway Administration.Title from PDF title page (viewed on April 14, 2022)."FHWA-OR-RD-22-11"--Technical report documentation page.This archived document is maintained by the State Library of Oregon as part of the Oregon Documents Depository Program. It is for informational purposes and may not be suitable for legal purposes.Includes bibliographical references.Sponsored by Oregon Dept. of Transportation, Research Section, Federal Highway Admin.Mode of access: Internet from the Oregon Government Publications Collection.Text in English.Final Repor
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