361,196 research outputs found
The relationship of the ADP-ribosylating enzyme from S. solfataricus with DING proteins and its intracellular localization
No full textThe PARPSso thermoprotein from Sulfolobus solfataricus has been identified as a PARP-like enzyme that cleaves -NAD+ to synthesize oligomers of ADP-ribose and cross-reacts with polyclonal anti-PARP-1 catalytic site antibodies. Despite the biochemical properties that allow to correlate it to PARP enzymes, the N-terminal and partial amino acid sequence suggest the sulfolobal enzyme belongs to a different class of enzymes, the DING proteins. Considering the high sequence identity with the human DING protein HPBP and the lack of a nucleotide coding sequence in both human and sulfolobal genomes, we hypothesized that PARPSso might share other features with the human DING. Further analysis of PARPSso amino acid sequence addressed the research towards studying other possible similarities between human and sulfolobal protein and then to explain how PARPSso correlates with canonic PARPs. For the latter question, the peculiar behaviour of the thermozyme, that is biochemically, but not structurally related to the classic PARPs, stimulated to investigate by computational analysis and databank, whether the protein might be phylogenetically related to any already known PARP amino acid sequence.
Moreover, immunochemical and enzymatic crossed analyses were performed to establish whether purified HPBP and PARPSso have common immunoreactive and functional behaviour.
The second part of the research was focused on the localization of PARPSso within the sulfolobal cell. Our interest to this item arose from the property of some DING proteins to be membrane bound, suggested to work as membrane transporters. On the other hand, from previous studies, it is known that PARPSso is only partially solubilized from the starting cell homogenate provided by ICMIB (CNR), and the soluble enzyme is strictly associated with DNA. In this thesis work, whole cells collected by centrifugation from culture medium were subjected to a different extraction procedure. This procedure included also experimental conditions used to differentiate between soluble (i.e. cytoplasmic) and insoluble (i.e. membrane-bound) protein fractions. PARPSso and DNA distributions were determined by enzyme assay, immunoblotting and agarose gel electrophoresis. Reciprocal interactions of thermozyme, nucleic acid and membrane lipids were investigated with different techniques and methodologies (nucleoid preparation, fluorescence binding assays, fluorescence microscopy analysis)
Nanostructured optical fibre tapers and related applications
No full textIn the last decade, optical fibre tapers have attracted considerable interest because they offer a variety of enabling properties, including large evanescent fields, flexibility, configurability, high confinement, robustness and compactness. These distinctive features have been exploited in a wealth of applications ranging from telecommunication devices to sensors, from optical manipulation to high-Q resonators. Nanostructures on the optical fibre tapers are very promising since the size of the device can be extremely small. With the development of nanostructuring methods, sub-wavelength feature sizes have been achieved. In this thesis, nanostructured optical fibre tapers and some related applications are discussed.Light confinement is limited by diffraction and the minimum spot size is related to the light wavelength. In this thesis, light confinement in two and three dimensions is proposed and achieved with two typologies of nanostructured optical fibre tapers. The first group of devices exploits plasmons excited at the optical fibre tips to obtain high transmissivity, and confine light to a sub-wavelength dimension. Optical fibre tips were designed according to numerical simulations and coated by a layer of gold; an extremely small aperture was then opened at the tip apex. The experimental characterization and simulation results showed their improved transmission efficiency (higher than 10-2) and thermal expansion measurements showed no shape changes could be detected within the accuracy of the system (~2 nm) for 9 mW injected powers. Effective confinements to 10 nm or smaller can be envisaged by decreasing the aperture size and slope angle. Application of this small spot size source can include scanning near-field optical microscope, optical recording, photolithography and bio-sensing.The second group achieves three dimensional light confinement exploiting a Fabry-Perot microcavity formed by a microfibre grating similar to those used in distributed feedback lasers. Microfibres were patterned using a Focused Ion Beam (FIB) system. In this structure, the microcavity provides longitudinal light confinement, whereas air dielectric guiding by the microfibre provides diffraction limited confinement in the other two dimensions. Due to the high refractive index contrast between silica and air, strong reflection can be obtained by only dozens of notches. This device can be used for a wide range of applications, e.g. sensing and triggered single-photon sources.Light confinement in nanostructured optical fibre tapers was exploited in a micrometric thermometer. A compact thermometer based on a broadband microfibre coupler tip showed a dynamic range spanning from room temperature to 1511ºC with a response time of tens of microseconds. This is the highest temperature measured with a silica optical fibre device. An average sensitivity of 11.96 pm/ºC was achieved for a coupler tip with ~2.5 µm diameter. A resolution of 0.66ºC was achieved for a coupler tip diameter of ~12.6 µm. Better resolution can be achieved with smaller size microfibre coupler tips.Optical fibre tapers are commonly used to couple light to selected resonator modes. Here FIB was used to inscribe microgrooves on optical Bottleneck Microresonator (BMR) surfaces to excite selected whispering gallery modes. By monitoring the transmission spectrum of the optical fibre taper, substantial spectral clean-up was obtained in appropriately scarred BMRs. Single high-Q mode operation can be achieved by either using two asymmetrical perpendicular scars or placing the grooves closer to the BMR centre, providing the potential for high performance sensors and other optical devices.Finally, strong three dimensional localization has been achieved in Plasmonic Slot Nano-Resonators (PSNRs) embedded in a gold-coated optical fibre tapers. Different shapes PSNRs, embedded in thin gold metal film coated plasmonic microfibre, were numerically investigated. The intensity enhancement (in excess of 106) and the resonance wavelength depend on both the PSNR and microfibre dimensions. Theoretically and experimentally, the transversal excitation of a rectangular PSNR embedded in a thin gold film coated plasmonic fibre tip was discussed for the first time, and showed high localization and strong enhancement (7.24×103). This device can find a wide range of applications such as surface-enhanced Raman scattering, optical filtering, spectroscopy and bio-sensing
Diffusive author(s), cohesive author: Analysis of S/N (1994)
No full textThis study indicates the ways in which various aspects of the author(s) are brought forth in Dumb type’s performance art, the S/N production. Previous research has suggested a non-hierarchical organization of Dumb type and the absence of a “privileged author” in Dumb type’s collaborative work, S/N. However, the results that I have investigated from member’s interviews on the creative process of S/N along with my analysis of the recorded images of S/N, indicate a different aspect of the author(s). First, S/N was created through, so to speak, the collective ideas of the members of Dumb type. Further, S/N has at least nine quotations from previous performances, installations, and printed writings, besides the work-in-progress technique. Explicating one of the “author functions” as given by Michel Foucault, each text has plural subjects of the author. However, it has been revealed from members’ interviews that Teiji Furuhashi had a decision-making role in selecting the members’ ideas within the performance. Since then, S/N has had plural subjects of creation; however, Furuhashi is one of the subjects of creation along with the “privileged author.” S/N has plural authors (diffusive authors) yet at the same time, it has a “privileged author,” Teiji Furuhashi (cohesive author)
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Ding, E J, 407602
No full textThis record was harvested from a previous catalogue system and will be withdrawn in 2025. Information in this record may be superseded or incomplete. Visit this record in UMA's new catalogue at: https://archives.library.unimelb.edu.au/nodes/view/381874Surname: DING. Given Name(s) or Initials: E J. Military Service Number or Last Known Location: 407602. Missing, Wounded and Prisoner of War Enquiry Card Index Number: 47248.211944
Item: [2016.0049.14167] "Ding, E J, 407602
Ding–Iohara–Miki symmetry of network matrix models
No full textAbstractWard identities in the most general “network matrix model” from [1] can be described in terms of the Ding–Iohara–Miki algebras (DIM). This confirms an expectation that such algebras and their various limits/reductions are the relevant substitutes/deformations of the Virasoro/W-algebra for (q,t) and (q1,q2,q3) deformed network matrix models. Exhaustive for these purposes should be the Pagoda triple-affine elliptic DIM, which corresponds to networks associated with 6d gauge theories with adjoint matter (double elliptic systems). We provide some details on elliptic qq-characters
The diffeotopy group of S-1 x S-2 via contact topology
No full textAs shown by Gluck in 1962, the diffeotopy group of S-1 x S-2 is isomorphic to Z(2) circle plus Z(2) circle plus Z(2). Here an alternative proof of this result is given, relying on contact topology. We then discuss two applications to contact topology: (i) it is shown that the fundamental group of the space of contact structures on S-1 x S-2, based at the standard tight contact structure, is isomorphic to Z; (ii) inspired by previous work of Fraser, an example is given of an integer family of Legendrian knots in S-1 x S-2#S-1 x S-2 (with its standard tight contact structure) that can be distinguished with the help of contact surgery, but not by the classical invariants (topological knot type, Thurston-Bennequin invariant, and rotation number).http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000280156000012&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701MathematicsSCI(E)7ARTICLE41096-111214
A Generalization of the Tang-Ding Binary Cyclic Codes
Full text linkCyclic codes are an interesting family of linear codes since they have
efficient decoding algorithms and contain optimal codes as subfamilies.
Constructing infinite families of cyclic codes with good parameters is
important in both theory and practice. Recently, Tang and Ding [IEEE Trans.
Inf. Theory, vol. 68, no. 12, pp. 7842--7849, 2022] proposed an infinite family
of binary cyclic codes with good parameters. Shi et al. [arXiv:2309.12003v1,
2023] developed the binary Tang-Ding codes to the -ary case. Inspired by
these two works, we study -ary Tang-Ding codes, where . Good
lower bounds on the minimum distance of the -ary Tang-Ding codes are
presented. As a by-product, an infinite family of -ary duadic codes with a
square-root like lower bound is presented
Modern clinical application of Ding 1541
No full textFor hundreds of years, Ding 1541 is used in traditional Chinese medicine (TCM) for the treatment of numerous ailments, including qi and blood stasis-related disorders. It showed good efficacy in relieving irregular menstruation, dysmenorrhea, metrorrhagia, and metrostaxis. Now, Ding 1541 has been widely used in various conditions, including primary dysmenorrhea, menstrual disorder, endometriosis, infertility, premenopausal syndrome, polycystic ovary syndrome, and premature ovarian failure. Compared with western medicine treatment, Ding 1541 has showed some advantages. In this review, we tried to summarize the underlying mechanisms for Ding 1541's effects. We collected clinical data and briefly analyzed these diseases based on the theories of TCM and the pathology. These data suggested that the underlying mechanisms of Ding 1541 involved increasing uterine blood supply, decreasing menstrual blood prostaglandin F2α level, improving the immune, and enhancing the function of the hypothalamus-pituitary-ovary, etc
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