32 research outputs found
Structure/function analysis of Type I interferons
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Application of a PEG/salt aqueous two-phase partition system for the recovery of monoclonal antibodies from unclarified transgenic tobacco extract
No full textInternational audienceAqueous two phase partition systems (ATPS) have been widely used for the separation of large variety of biomolecules. In the present report, the application of a polyethyleneglycol/phosphate (PEG/Pi) ATPS for the separation of anti-HIV monoclonal antibodies 2G12 (mAb 2G12) and 4E10 (mAb 4E10) from unclarified transgenic tobacco crude extract was investigated. Optimal conditions that favour opposite phase partitioning of plant debris/mAb as well as high recovery and purification were found to be 13.1% w/w [PEG 1500], 12.5% w/w [Pi] at pH 5 with a phase ratio of 1.3 and 8.25% w/w unclarified tobacco extract load. Under these conditions, mAb 2G12 and mAb 4E10 were partitioned at the bottom phosphate phase with 85 and 84% yield and purification 2.4- and 2.1-fold, respectively. The proposed ATPS was successfully integrated in an affinity-based purification protocol, using Protein A, yielding antibodies of high purity and yield. From this study, ATPS was shown to be suitable for initial protein recovery and partial purification of mAbs from unclarified transgenic tobacco crude extract
Development of an aqueous two-phase partitioning system for fractionating therapeutic proteins from tobacco extract
No full textDesign and implementation of monitoring and evaluation of health care system: The paradigm of primary health care services users in Greece
No full textDesign and implementation of monitoring and evaluation of healthcare organization management
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Random geometric structures on high genus surfaces
No full textIn this dissertation, we study two problems involving random geometries on surfaces. Firstly, we study Benjamini-Schramm convergence of high genus translation surfaces with area comparable to genus, as genus tends to infinity. We prove convergence and describe its limit, which we call a Poisson translation plane. This project was completed in collaboration with Lewis Bowen and Kasra Rafi. Secondly, we study quasi-Fuchsian space and prove that the natural measure on quasi-Fuchsian space, which we refer to as Goldman-Platis measure, is cofinite with respect to the mapping class group action. We focus on two particular subsets of the Fuchsian locus in quasi-Fuchsian space, which we call the Bers and Thurston Fuchsian lenses. We prove that Goldman-Platis measure escapes these neighborhoods as genus tends to infinity, and we derive necessary and sufficient conditions in terms of the Weil-Petersson and Teichmüller metrics for a pair (X,Y) to map into the Bers Fuchsian lens under the Bers simultaneous uniformization. This second project is solely the author's work.Mathematic
Correction: Hall et al. Oral and Poster Abstracts of the 13th ISNS European Regional Meeting. Int. J. Neonatal Screen. 2025, 11, 21
No full textThe authors wish to make the following correction to their paper published in the International Journal of Neonatal Screening [...
Dépistage néonatal en Europe
No full textLe dépistage néonatal a débuté en Europe dans les années 1960 avec celui de la phénylcétonurie. Le nombre de maladies dépistées a, par la suite, augmenté progressivement, de manière plus marquée à la fin des années 1990 avec l’arrivée de la spectrométrie de masse en tandem (MS/MS) qui a permis le dépistage de 40 à 50 maladies sur une seule goutte de sang séché. Les ajouts les plus récents à cette liste de maladies (mucoviscidose, déficits immunitaires combinés sévères et atrophie musculaire spinale) ont été rendus possibles grâce à la génétique moléculaire. À partir des informations provenant de 51 pays d’Europe, nous décrivons dans cette revue l’évolution du dépistage entre 2010 et 2020, ainsi que les progrès réalisés pendant cette période, tout en soulignant les aspects qui méritent d’être améliorés. Des progrès pourront en effet être accomplis grâce aux échanges d’informations et, pour certains pays, en tirant profit de l’expérience acquise dans des pays voisins. La plupart des programmes de dépistage mis en place dans l’Europe « géographique » au cours de cette période ont gagné en maturité en termes méthodologiques (modernisation des techniques) et en termes quantitatifs (augmentation du nombre des maladies dépistées). Ces développements nous montrent que la collaboration entre les différentes organisations s’accélère en Europe. Ce n’est qu’en travaillant ensemble que nous pourrons identifier en temps opportun les nouveau-nés atteints d’une des nombreuses maladies rares détectables et prendre les mesures qui s’imposent
How Long Does (S)He Have? Retrospective Analysis of Outcomes After Palliative Extubation in Elderly, Chronically Critically Ill Patients
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