197,155 research outputs found
Application of Denaturing Gradient Gel ElectrophoresisApplication of Denaturing Gradient Gel Electrophoresis(DGGE) Methods on Parent-Offspring Relationship of the Coral Pocillopora damicornis
No full textDGGE (Denaturing Gradient Gel Electrophoresis) is the most powerful methods for mutation detection currentlyavailable. In DGGE, DNA fragments of the same length but with different sequences can be separated. Thesensitivity of DGGE to slight sequence differences is high since single base changes could be observed. Therehas been a debate about whether planulae of the coral Pocillopora damicornis are produced sexually or asexually.If produced sexually, planulae are expected to be genetically different from each other and also from theirparents. In order to detect possible genetic difference between planulae and their parents, DGGE analysis ofITS2 region of rDNA was used. If there are genetic differences, it is proved that planulae are producedsexually. A total 49 adult colonies and 78 planulae from 11 localities were used for analysis. However, only in2 families (Bise01#02 and Bise02#03YL) showed different DGGE profile, suggesting genetic difference betweenparent and offspring. The attempt to detect genetic difference in planulae of P. damicornis and their parentsusing DGGE method was not completely success since DGGE method can not prove clearly the genetic differencebetween parent and offspring. However, the possibility that DGGE method is applicable for studying coral canbe suggested. PCR-DGEE amplification may perform with new STR (short tandem repeat) polymorphic loci ofP. damicornis that currently found to answer whether planulae are produced sexually or asexually.
DGGE (Denaturant Gradient Gel Electrophoresis) merupakan salah satu teknik akurat untuk mengetahui mutasiDNA. Sensivitas DGGE sangat tinggi, karena dengan menggunakan DGGE, fragmen DNA yang memiliki panjangbasa yang sama dapat dipisahkan hanya berdasarkan perbedaan satu basa penyusunnya saja. Model reproduksikarang Pocillopora damicornis telah lama menimbulkan perdebatan. P. damicornis dilaporkan memproduksi planulaesecara seksual dan aseksual. Jika diproduksi secara seksual, planula akan berbeda secara genetik denganinduknya. Analisa DGGE dikombinasikan marker ITS2 dari rDNa digunakan untuk mendeteksi perbedaan tersebut.Jika terdapat perbedaan genetik, dapat disimpulkan planulae diproduksi secara seksual. Analisa dilakukan terhadap53 koloni dan 78 planulae dari 11 lokasi yang berbeda. Dua famili (Bise01#02 and Bise02#03YL) mempunyaiprofil DGGE berbeda antara planulae dan induknya. Hal ini menunjukkan adanya perbedaan susunan gen antaraplanulae dan induknya dan kemungkinan produksi planulae dilakukan secara seksual. Namun demikian, kombinasianalisa DGGE dan marker ITS2 dari rDNA kurang memberi hasil memuaskan. Saat ini telah ditemukan STR (shorttandem repeat) spesifik untuk P. damicornis. Kombinasi penggunaan amplifikasi PCR-DGGE dengan STR diharapkanlebih akurat untuk menjawab permasalahan produksi planulae pada karang P. damicornis
Intensity-duration-frequency analysis of energy supply droughts from renewable energy sources across an Alpine transect.
No full textRestrukturisasi B U M N di Indonesia privatisasi atau korporatisasi/ Diah
No full textx, 355 hal.: tab.; 21 cm
Restrukturisasi B U M N di Indonesia privatisasi atau korporatisasi/ Diah
No full textx, 355 hal.: tab.; 21 cm
Restrukturisasi B U M N di Indonesia privatisasi atau korporatisasi/ Diah
No full textx, 355 hal.: tab.; 21 cm
Restrukturisasi B U M N di Indonesia privatisasi atau korporatisasi/ Diah
No full textx, 355 hal.: tab.; 21 cm
Restrukturisasi B U M N di Indonesia privatisasi atau korporatisasi/ Diah
No full textx, 355 hal.: tab.; 21 cm
Dr. Duane M. Jackson, Morehouse College, July 2011
No full textThis video is a conversation with Dr. Duane M. Jackson. Dr. Jackson talks about his paper, "Recall and the Serial Position Effect: The Role of Primacy and Recency on Accounting Students' Performance." Jackie Daniel, AUC Woodruff Library, is the interviewer
"Reflections on the subject of Emigration from Europe with a view to Settlement in the United States" By M. Carey.
No full text"Reflections on the subject of Emigration from Europe with a view to Settlement in the United States: containing bried sketches of the moral and political character of those states.
By M. Carey, member of the American philosophical, and of the American Antiquarian Society, and author of The Olive Branch, Cindiciae Hibernicae, essays on banking, on political economy, and on internal improvement.
To which are now added the English editor's comments on the subject; together with Important Advice to Emigrants, and Cautions Against Impositions Practiced in the Outports
Dispelling the Myths Behind First-author Citation Counts
No full textWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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