51 research outputs found
Protein Profile and Hematological Parameters of Mice post Injected with Irradiated Plasmodium berghei
Get PDFIndonesia had a high number of malaria cases where the treatments are only perceived by 10% of patients in health facilities. The resistance of Plasmodium sp. to drugs causes the difficulty of controlling malaria. The use of irradiation to produce malaria vaccine material is being explored until nowadays. In previously study that dose rate of gamma irradiation delivered to parasite was at the low dose rate (380 Gy/hour) which produced several doses that not be able to attenuate the parasite. This research aimed to increase dose rate (740 Gy/hour) and to determine the dose irradiation effect of 150-250 Gy to the protein profile, erythrocyte and leukocyte numbers in mouse blood infected by P. berghei erythrocytic stage. Results showed that there was an influence of dose rate of 740 Gy/hour for doses of 150-250 Gy in treated mice and no difference in erythrocyte, leucocyte and body weight with negative control. Dose of 150 Gy was the optimal dose to attenuate parasites, whereas doses more than 150 Gy effectively killed the parasites. The changes of protein profile marked by the appearance of higher number of bands at molecular weight of 30-37 kDa that predicted as merozoite surface protein of P. berghei and/or antibodies elicited in mouse.How to CiteRosadi, I., Syaifudin, M., & Elfidasari, D. (2016). Protein Profile and Hematological Parameters of Mice post Injected with Irradiated Plasmodium berghei. Biosaintifika: Journal of Biology & Biology Education, 8(2), 155-164</p
Prey diversity for Three Species Herons in Pulau Dua Nature Reserve Serang District, Banten Province
No full textKORELASI RAGAM AKTIVITAS TERHADAP KEBERHASILAN MAKAN TIGA JENIS KUNTUL DI CAGAR ALAM PULAU DUA TELUK BANTEN, SERANG
Get PDFCorrelation in Activity Variation to Feeding Success on Three Species Herons at Pulau Dua Nature Reserve Banten\u27s Bays, Serang. This research intent to know activity manner influence that did by three species herons for feeding success at Pulau Dua Nature Reserve, Banten Bay, Serang. Observational method that is utilized is direct observing with backed up by picture taking utilizes observation method is focal. Analysis draws to be done by scan sampling method\u27s. Data analyzed by correlation terminologically Spearmann by programs SPSS. Result observationally to point out that activity type was done upon eating is step, strike, catch and devours prey. Result tests Spearmann\u27s correlation proves that activity manner a large part gets positive correlation with feeding success for three species herons depend on different feeding location. Just activity stop and not active just that gets negative correlation with feeding success on three species heron
KORELASI RAGAM AKTIVITAS TERHADAP KEBERHASILAN MAKAN TIGA JENIS KUNTUL DI CAGAR ALAM PULAU DUA TELUK BANTEN, SERANG
Get PDFCorrelation in Activity Variation to Feeding Success on Three Species Herons at Pulau Dua Nature Reserve Banten\u27s Bays, Serang. This research intent to know activity manner influence that did by three species herons for feeding success at Pulau Dua Nature Reserve, Banten Bay, Serang. Observational method that is utilized is direct observing with backed up by picture taking utilizes observation method is focal. Analysis draws to be done by scan sampling method\u27s. Data analyzed by correlation terminologically Spearmann by programs SPSS. Result observationally to point out that activity type was done upon eating is step, strike, catch and devours prey. Result tests Spearmann\u27s correlation proves that activity manner a large part gets positive correlation with feeding success for three species herons depend on different feeding location. Just activity stop and not active just that gets negative correlation with feeding success on three species heron
PENGARUH PERBEDAAN LOKASI MENCARI MAKAN TERHADAP KERAGAMAN MANGSA TIGA JENIS KUNTUL DI CAGAR ALAM PULAU DUA SERANG: Casmerodius albus, Egretta garzetta, Bubulcus ibis
Get PDFPrey diversity influenced by the different or foraging areas for three species herons in Cagar Alam Pulau Dua Serang. This research is to know the influenced of foraging areas to prey diversity for three species herons in Cagar Alam Pulau Dua Serang, Banten Province. This research have been done at feeding area for the herons around Cagar Alam Pulau Dua. The data were from film shooting use Focal Observation Methods, while the film shooting analyze use scan sampling methods. It’s explain that Casmerodius albus and Egretta garzetta have preferency to select the mudflat and fisheries around CAPD as foranging location because they preferency to select fish as they main prey. Bubulcus ibis who select to using rice-field and grassland as a foraging location have preferency to select insects as a main prey. The selection of foraging location for three species herons are depend on the spesific kind and size of preys for them. The preferency for Casmerodius albus to feedinginest is mudflat with the mayority prey are big fish, with the size of more than 15 cm. Egretta garzetta have preferency to select fisheries because they main prey are fish with size of about 3-5 cm. Bubulcus ibis is prefer to feeding in grassland because they eat the insect with the preferences prey size smaller than 3 cm
KORELASI RAGAM AKTIVITAS TERHADAP KEBERHASILAN MAKAN TIGA JENIS KUNTUL DI CAGAR ALAM PULAU DUA TELUK BANTEN, SERANG
No full textCorrelation in Activity Variation to Feeding Success on Three Species Herons at Pulau Dua Nature Reserve Banten's Bays, Serang. This research intent to know activity manner influence that did by three species herons for feeding success at Pulau Dua Nature Reserve, Banten Bay, Serang. Observational method that is utilized is direct observing with backed up by picture taking utilizes observation method is focal. Analysis draws to be done by scan sampling method's. Data analyzed by correlation terminologically Spearmann by programs SPSS. Result observationally to point out that activity type was done upon eating is step, strike, catch and devours prey. Result tests Spearmann's correlation proves that activity manner a large part gets positive correlation with feeding success for three species herons depend on different feeding location. Just activity stop and not active just that gets negative correlation with feeding success on three species herons. Keywords: activities, influence, feeding, success, heron
KORELASI RAGAM AKTIVITAS TERHADAP KEBERHASILAN MAKAN TIGA JENIS KUNTUL DI CAGAR ALAM PULAU DUA TELUK BANTEN, SERANG
Get PDFCorrelation in Activity Variation to Feeding Success on Three Species Herons at Pulau Dua Nature Reserve Banten\u27s Bays, Serang. This research intent to know activity manner influence that did by three species herons for feeding success at Pulau Dua Nature Reserve, Banten Bay, Serang. Observational method that is utilized is direct observing with backed up by picture taking utilizes observation method is focal. Analysis draws to be done by scan sampling method\u27s. Data analyzed by correlation terminologically Spearmann by programs SPSS. Result observationally to point out that activity type was done upon eating is step, strike, catch and devours prey. Result tests Spearmann\u27s correlation proves that activity manner a large part gets positive correlation with feeding success for three species herons depend on different feeding location. Just activity stop and not active just that gets negative correlation with feeding success on three species heron
PENGARUH PERBEDAAN LOKASI MENCARI MAKAN TERHADAP KERAGAMAN MANGSA TIGA JENIS KUNTUL DI CAGAR ALAM PULAU DUA SERANG: Casmerodius albus, Egretta garzetta, Bubulcus ibis
No full textPrey diversity influenced by the different or foraging areas for three species herons in Cagar Alam Pulau Dua Serang. This research is to know the influenced of foraging areas to prey diversity for three species herons in Cagar Alam Pulau Dua Serang, Banten Province. This research have been done at feeding area for the herons around Cagar Alam Pulau Dua. The data were from film shooting use Focal Observation Methods, while the film shooting analyze use scan sampling methods. It’s explain that Casmerodius albus and Egretta garzetta have preferency to select the mudflat and fisheries around CAPD as foranging location because they preferency to select fish as they main prey. Bubulcus ibis who select to using rice-field and grassland as a foraging location have preferency to select insects as a main prey. The selection of foraging location for three species herons are depend on the spesific kind and size of preys for them. The preferency for Casmerodius albus to feedinginest is mudflat with the mayority prey are big fish, with the size of more than 15 cm. Egretta garzetta have preferency to select fisheries because they main prey are fish with size ofabout 3-5 cm. Bubulcus ibis is prefer to feeding in grassland because they eat the insect with the preferences prey sizesmaller than 3 cm.Keywords: feeding, location, herons, mudflat, rice-field, grass-land, fisherie
Peran burung air liar dalam penyebaran virus avian influenza subtipe H5N1 di Cagar Alam Pulau Dua
No full textWaterfowls such as ducks, muscovy, and swans are natural reservoir of influenza virus subtype H5N1. In natural reservoir, the AI virus is Low Pathogenic Avian Influenza (LPAI). Instead of domestic waterfowls, wild waterbird species could also have a role in spreading AI subtype H5N1 virus. So far LPAI have been isolated from 105 species of wild birds, most of them were from Anseriformes and Charadiiformes order. Presumption that wild birds and migratory birds have a role in spreading AI virus, has not been proven until now because the intensive research has not been done. The mortality rate of wild waterbirds that reported in several countries, had not clearly explained the cause of death. The objectives of this research were (1) to estimate the seroprevalence of AI virus subtype H5N1 from residence wild waterbirds, in Pulau Dua Nature Reverse; (2) to identify the Mx gene as resistant anti viral gene from AI virus in wild waterbirds; (3) to identify the presence of AI virus subtype H5N1 on residence waterbirds in Pulau Dua Nature Reverse, also to observe the potential of wild waterbirds as reservoir for spreading AI virus subtype H5N1 The serological test to detect the presence of antibody antiviral AI virus subtype H5N1 was used to determine the exposure of AI virus subtype H5N1 among wild waterbirds in CAPD. Since antibody is the substance that appear in individual exposed when they are exposed by the strange antigen. The serosurveillans from wild waterbirds in CPAD had been done in 2008, 2010 and 2011. Serological test by Haemagglutinin-inhibition (HI) showed that six from nine species of residence wild waterbirds in CPAD produced antibody AI virus H5N1. It is explain that that those species were exposed by AI virus subtype H5N1 with low antibody titer. The low antibody titer can be caused by several factors such as AI virus exposure occurring indirectly, there was a small number of virus that exposure to wild waterbirds, the exposure time was very short, and the virus was low pathogenic. Indirect transmission occurred through the water as the source of drinking water for wild and domestic waterbirds, through saliva and nasal secretions and feces. Water as a media for transmission virus where there were interactions between wild waterbirds, waterfowls and domestic birds. Mx protein as part of dynamin super family (GTP ase) is a part of the innate immune system. Mx proteins were known to have the ability to inhibit the replication of various viruses. Mx protein coded by Mx genes that specifically anti-viral influenza in several animals. Mx gene was the one of the several genes that regulated myxovirus resistance. DNA of eight wild waterbirds species and three domestic birds (chicken, duck and Muscovy) around CAPD were examined by PCR using NE-F2 and NER2/ R primer obtaining PCR product with a length of 100 bp band. It indicated that most birds could have 100 bp band. These results explained that the Mx gene in residence wild waterbirds from CAPD could be the same as in the chicken, muscovy and duck. The results of PCR-RFLP in wild waterbirds and domestic waterfowls using the restriction enzyme RsaI showed band in 73 bp length (only have GG genotype). It informed that there was no polymorphism because all fragments display only one band (73bp ) in wild waterbirds and domestic waterfowls (muscovy and duck) around CAPD showed. In chickens, the 73 bp fragment (GG genotype) showed low antivirus activity because it has sensitive Mx gene (allele Mx-) and responsive to AI virus subtype H5N1 attack. This is not happened to ducks, muscovy, and wild waterbirds, although they have only one band (Mx- alleles). It was not caused death or clinical symptoms by the exposure of AI virus subtype H5N1. The virus identification by RT-PCR with primer used in identification of HA subtypes H5. Forward primers was H5 Kha-1 and reverse H5 Kha-3primer pair. The identification results of HA subtypes H5 in residence wild waterbirds in CAPD showed that the H5 subtype was not detected in these wild waterbirds. This was showed by the absence of positive bands that indicate the presence of the AI virus subtypes H5 in wild waterbirds. The absence of AI virus subtype H5N1 in nine species of residence wild waterbirds including Ciconiformes and Pelecaniformes order showed that these birds do not have the potential as a reservoir for AI virus subtype H5N1. It gives the information that birds are not a suitable host for the cultured AI virus subtype H5N1, so that there were no viral shedding into the environment from residence wild waterbirds
PENGARUH PERBEDAAN LOKASI MENCARI MAKAN TERHADAP KERAGAMAN MANGSA TIGA JENIS KUNTUL DI CAGAR ALAM PULAU DUA SERANG: Casmerodius albus, Egretta garzetta, Bubulcus ibis
Get PDFPrey diversity influenced by the different or foraging areas for three species herons in Cagar Alam Pulau Dua Serang. This research is to know the influenced of foraging areas to prey diversity for three species herons in Cagar Alam Pulau Dua Serang, Banten Province. This research have been done at feeding area for the herons around Cagar Alam Pulau Dua. The data were from film shooting use Focal Observation Methods, while the film shooting analyze use scan sampling methods. It’s explain that Casmerodius albus and Egretta garzetta have preferency to select the mudflat and fisheries around CAPD as foranging location because they preferency to select fish as they main prey. Bubulcus ibis who select to using rice-field and grassland as a foraging location have preferency to select insects as a main prey. The selection of foraging location for three species herons are depend on the spesific kind and size of preys for them. The preferency for Casmerodius albus to feedinginest is mudflat with the mayority prey are big fish, with the size of more than 15 cm. Egretta garzetta have preferency to select fisheries because they main prey are fish with size of about 3-5 cm. Bubulcus ibis is prefer to feeding in grassland because they eat the insect with the preferences prey size smaller than 3 cm
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