1,873 research outputs found
Evaluation of the first automated thyroglobulin assay
No full textThe aim of this study was to investigate technical and analytical performance of the first automated thyroglobulin (Tg) assay (DPC-Immulite(R); Diagnostic Products Corporation, Los Angeles, USA). In imprecision studies using several human serum pools ranging from 21 to 58 replicates, a coefficient of variation of 9.0 % was obtained at a mean Tg concentration of 0.84 ng/ml and of 6.1 % at a Tg concentration of 62.1 ng/ml. In a method comparison with a non-automated assay (BRAHMS LUMItest Tg(R), BRAHMS, Berlin, Germany) using 383 sera of 303 patients with thyroid carcinoma, regression analysis according to Passing and Bablock yielded in the following equation: Immulite Tg=1.6 x BRAHMS Tg - 0.1 ng/ml (Pearson's r=0.979). Sera obtained from 59 patients with thyroid carcinoma enabled comparative follow-up studies; in all cases qualitative agreement was found with regard to increase or decrease of serum Tg; in eight cases, however, Tg was detected with the Immulite assay but not with the BRAHMS assay. Further follow-up proved the presence of thyroid tissue in these patients. From these and further methodological data (dilution linearity, interference studies, carry-over study, high-dose hook properties, and short report time) it is concluded that the DPC-Immulite Tg assay meets the requirements of routine diagnostic use
Synthesis and characterization of metallized metal-organic frameworks and their use in the production of vanillin
No full textTarımsal atıklar yenilenebilir hammadde kaynaklarındandır ve bu atıkların katma değeri yüksek ürünlere dönüştürülmesi küresel ısınmayla birlikte daha bir önem kazanmıştır. Bilindiği üzere Rize ve çevre illerin en önemli tarımsal ürünü çaydır ve çay üretimi sırasında her yıl yaklaşık 30 000 ton fabrika çay atığı ortaya çıkmaktadır. Amaca yönelik sentezlenebilmeleri ve kullanım alanlarının çeşitliliği ile öne çıkan bir malzeme türü olan metal-organik kafes yapılar (MOFs) bilim dünyasının hızla gelişen malzemeleri arasındadır. Benzen-1,4′-dikarboksilik asidin zirkonyum (IV) metal iyonlarıyla verdiği tepkime sonucu hazırlanabilen yüksek kimyasal ve ısısal kararlılığa sahip UiO-66 (UiO= University of Oslo) tipi metal-organik kafes yapılar bu malzemeler arasında üretim ve kullanım kolaylığı yönüyle tercih edilmektedir. Tez kapsamında UiO-66 tipi yeni metal-organik kafes yapılar hazırlandı ve sentez sonrası metallendirilerek demir, bakır, demir+bakır içeren kafes yapılara dönüştürüldü. Sentezlenen kafes yapılar elementel analiz, ICP, fourier transform Infrared (FTIR) spektroskopisi, termik analiz (TG), X-Işınları toz kırınımı, taramalı elektron mikroskobu (SEM), enerji dağılımlı X-Işını spektroskopisi (EDS) gibi çeşitli yöntemler ile karakterize edildi. Yüzey alanları ve gözeneklilik özellikleri belirlendi. Hazırlanan metal-organik kafes yapılar lignin model bileşik olarak seçilen ferulik asitin ve çay atığından elde edilen ligninin vaniline dönüşüm reaksiyonlarında katalizör olarak kullanıldı. Sonuçlar yüksek performanslı sıvı kromotoğrafisi, HPLC ile kontrol edildi.Agricultural wastes are renewable raw material sources and conversion of these wastes to high value added products has gained more importance with global warming. As it is known, tea is the most important agricultural product of Rize and the surrounding provinces and approximately 30 000 tons of factory tea waste is produced each year during tea production. Metal-organic frameworks (MOFs) which are the rapidly developing materials of the scientific world because of diversity of their application areas and tailored structures. UiO-66 (UiO = University of Oslo) type metal-organic frameworks which have high chemical and thermal stability can be prepared by the reaction of benzene-1,4′-dicarboxylic acid with zirconium (IV) metal ions are preferred in terms of production and ease of use among these materials. In this thesis, UiO-66 type new metal-organic frameworks were prepared and transformed into the iron, copper or iron+copper containing metal-organic frameworks via post-synthetic metallation. The synthesized metal-organic frameworks were characterized by various methods such as elemental analysis, ICP, fourier transform Infrared (FTIR) spectroscopy, thermal analysis (TG), X-ray powder diffraction, scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy (EDS). Further, their surface areas and porosity properties were determined. The prepared metal-organic frameworks were used as catalysts in vanillin conversion reactions of ferulic acid which selected as lignin model compound and lignin obtained from tea waste. Results were checked by high performance liquid chromatography, HPLC
Synthesis and Characterization of Magnesium Borate via Sol Gel Method and Electrospinning Method
No full textThe sol gel and electrospinning techniques were used to prepare the magnesium borate polyvinyl alcohol composite. The fibers of magnesium borate about 150 nm diameters, were synthesized from the inorganic organic composite fibers by high temperature calcination process. The synthesized magnesium borate nanofibers were structurally investigated by thermogravimetric differential thermal analysis TG DTA , Fourier transform infrared spectroscopy FT IR , X ray diffractometry XRD and Scanning electron microscopy SEM , respectively. Due to the Mg modification of the B2O3 network, incomplete crystallization of the samples was observed when heat treated at 800 °C. But, completely crystalline fibers were obtained after calcined at 1200 ° C. Esra Nur Dogana | Fatih Demir "Synthesis and Characterization of Magnesium Borate via Sol-Gel Method and Electrospinning Method" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-3 , April 2019, URL: https://www.ijtsrd.com/papers/ijtsrd21662.pd
Bulk viscosity in F(T, TG) gravity
No full textThe present paper is devoted to exploring the effect of bulk viscosity in the context of F(T, TG) gravity. We consider a time-dependent viscosity model with a particular expression of Hubble parameter. We evaluate viscous effective equation of state parameter for three well-known F(T, TG) models. The behavior of the accelerated expanding universe is explored graphically through the viscous equation of state parameter. This parameter indicates the phantom-dominated era as well as crosses the phantom divide line for all three models. We conclude that the universe shows a transition from quintessence to phantom region in the presence of bulk viscosity.The presentation of the authors' names and (or) special characters in the title of the pdf file of the accepted manuscript may differ slightly from what is displayed on the item page. The information in the pdf file of the accepted manuscript reflects the original submission by the author
Mercimekte AG ve AC Tekrarları ile Zenginleştirilmiş Kütüphanelerin TG ve TC Tekrarları ile Taranarak Yeni SSR Markörlerin Geliştirilmesi
No full textSimple sequence repeats (SSRs) markörler, bitkilerde genetik ve genomik araştırmalarda yaygın olarak kullanılan önemli moleküler gereçlerdir. Ancak ekonomik öneme sahip serin iklim baklagil bitkilerinden mercimekte (Lens culinaris Medik.) bugüne kadar geliştirilen SSR markörü sayısı oldukça sınırlı kalmıştır. SSR markörlerin bu eksikliği, mercimek moleküler ıslah çalışmalarını sınırlayan başlıca faktörler arasında yer almaktadır. Bu çalışmada, mercimek bitkisinde SSR markörü geliştirmek için AC ve AG tekrarlarınca zenginleştirilmiş kütüphanelere ait 288 klon TG ve TC tekrarlarınca taranarak yeni 15 adet SSR markörü geliştirilmiştir. Polimorfizm gösteren markörlerden toplamda 18 allel üretilmiş ve Lc-MCu54 markörü en polimorfik markör olarak belirlenmiştir. Geliştirilen bu markörler mercimek bitkisinde moleküler temelli pek çok çalışmaya katkı sağlayacak niteliktedir.</div
() Topology of unimolecular G-quadruplex adopted by dG(TG) and -ion-binding sites
No full text<p><b>Copyright information:</b></p><p>Taken from "NMR evaluation of ammonium ion movement within a unimolecular G-quadruplex in solution"</p><p>Nucleic Acids Research 2007;35(8):2554-2563.</p><p>Published online 4 Apr 2007</p><p>PMCID:PMC1895886.</p><p>© 2007 The Author(s)</p> The three binding sites are labeled as O, I and O. The guanine bases are shown as numbered rectangles, where cyan and magenta rectangles represent nucleobases in and conformation, respectively. () Birds-eye view of a ion above an individual G-quartet
Competition of the 13 bp substrate duplexes and the Tg-containing duplexes in the Endo III reaction
No full text<p><b>Copyright information:</b></p><p>Taken from "Synthesis and characterization of oligonucleotides containing 2′-fluorinated thymidine glycol as inhibitors of the endonuclease III reaction"</p><p>Nucleic Acids Research 2006;34(5):1540-1551.</p><p>Published online 17 Mar 2006</p><p>PMCID:PMC1409675.</p><p>© The Author 2006. Published by Oxford University Press. All rights reserved</p> The P-labeled substrates without fluorine were incubated with Endo III in the presence of the competitors containing Tg. The amounts of the nicked products (standardized to those without the competitors) were plotted against the concentrations of the competitor. Open circles, P-5-Tg + 5-Tg; filled circles, P-5-Tg + 5-Tg; open triangles, P-5-Tg + 5-Tg; filled triangles, P-5-Tg 5-Tg
DNA fusion gene vaccination mobilizes effective anti-leukemic cytotoxic T lymphocytes from a tolerized repertoire
No full textThe majority of known human tumor-associated antigens derive from non-mutated self proteins. T cell tolerance, essential to prevent autoimmunity, must therefore be cautiously circumvented to generate cytotoxic T cell responses against these targets. Our strategy uses DNA fusion vaccines to activate high levels of peptide-specific CTL. Key foreign sequences from tetanus toxin activate tolerance-breaking CD4+ T cell help. Candidate MHC class Ibinding tumor peptide sequences are fused to the C terminus for optimal processing and presentation. To model performance against a leukemia-associated antigen in a tolerized setting, we constructed a fusion vaccine encoding an immunodominant CTL epitopederived from Friend murine leukemia virus gag protein (FMuLVgag) and vaccinated tolerant FMuLVgag-transgenic (gag-Tg) mice. Vaccination with the construct induced epitopespecificIFN-c-producing CD8+ T cells in normal and gag-Tg mice. The frequency and avidity of activated cells were reduced in gag-Tg mice, and no autoimmune injury resulted. However, these CD8+ T cells did exhibit gag-specific cytotoxicity in vitro and in vivo. Also, epitope-specific CTL killed FBL-3 leukemia cells expressing endogenous FMuLVgag antigen and protected against leukemia challenge in vivo. These results demonstrate a simple strategy to engage anti-microbial T cell help to activate epitope-specific polyclonal CD8+ T cell responses from a residual tolerized repertoire
Aromatic () and imino () regions of H NMR spectrum of form of dG(TG) G-quadruplex at 298 K
No full text<p><b>Copyright information:</b></p><p>Taken from "NMR evaluation of ammonium ion movement within a unimolecular G-quadruplex in solution"</p><p>Nucleic Acids Research 2007;35(8):2554-2563.</p><p>Published online 4 Apr 2007</p><p>PMCID:PMC1895886.</p><p>© 2007 The Author(s)</p> Plot of 2D N–H HSQC spectrum (). The cross-peak corresponding to ions in bulk is labeled as B, while those residing at the binding sites within the G-quadruplex are labeled as O, O and I
Gradient interphases between high-Tg epoxy and polyetherimide for advanced joining processes
No full textAdhesive joining of carbon fibre reinforced polymer (CFRP) is cumbersome due to the careful surface preparation required and multiple validation steps to certify adhesion quality. Further these joints are often supplemented by mechanical fastenings add weight whilst also localising bearing stress. As an alternative technique, CFRP parts can be functionalized with thermoplastic surfaces during manufacture to enable cost-effective welding of composite structures. In the process of manufacturing the CFRP, curing an epoxy resin in the presence of the functionalising thermoplastic polymer can lead to local dissolution of the latter in the epoxy, followed by a reaction-induced phase separation. This results in a thermosetting-thermoplastic interphase featuring gradient concentrations and a multiphase morphology, which promotes load transfer between the thermosetting matrix and the thermoplastic joint. The aim of the work presented in this paper was to investigate interphase formation between high-Tg epoxy and polyetherimide (PEI) at different curing temperatures. The morphology was characterised using scanning electron microscopy and the composition of the interphase was quantified through Raman spectroscopy. The curing experiments indicated that temperature has a significant effect on the interphase morphology and led to two different biphasic morphologies which generally increased in size with increasing curing temperature. This suggests that the size of the gradient interphase can be tailored through the curing process, which is as a fundamental step in optimising the structural performance of welded joints with PEI-functionalized epoxy-based CFRPs.Aerospace Manufacturing TechnologiesStructural Integrity & Composite
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