1,721,067 research outputs found
Functional outcomes after treatment for prostate cancer
No full textLong-term functional outcomes after treatment for localized prostate cancer. [N Engl J Med. 2013
Zonulin immunolocalizationin human intestinal duodenal epithelium of patients with Systemic Nickel Allergy Syndrome (SNAS)
No full textClaudin-1 and Claudin-2 immunolocalization in human intestinal duodenal epithelium of patients
No full textClaudins (CLs) comprise a multi-gene family functionally involved in the tight junction barrier and aqueous pore formation and thereby play a key role in determining the permeability properties of epithelial cells. In particular, CL-1 is expressed ubiquitously in most tissues of the body, while CL-2 is highly expressed in leaky epithelial tissues, including the intestinal crypts.
The aim of this study was to investigate the immunolocalization of CL-1 and CL-2, transmembrane proteins identified as integral components of tight junction strands, in duodenal biopsies of control subjects and in patients with Systemic Nickel Allergy Syndrome (SNAS), characterized by the presence of systemic and gastrointestinal symptoms following nickel ingestion. In parallel the F-actin cytoskeletal organization was assessed in biopsy samples.
Staining with rhodamine phalloidin indicated that the junctional protein CL1 and CL2 are membrane cytoskeleton-associated. Confocal microscopic analysis of intestinal sections (5 μm) revealed an increased immunofluorescence signal for both CL1 and CL2 in subject sensitive to nickel sulfate compared to controls.
In conclusion CL1 and CL2 showed an increased expression in SNAS patient as assessed by immunofluorescence analysis. This could be associated with alteration of tight junction organization and permeability of the gastrointestinal barrier in SNAS diseas
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
LIMITED VERSUS EXTENDED PELVIC LYMPHADENECTOMY DURING ROBOT-ASSISTED RADICAL PROSTATECTOMY: IMPACT ON THE NUMBER OF NODES AND ON NODAL INVASION
No full textLIMITED VERSUS EXTENDED PELVIC LYMPHADENECTOMY DURING ROBOT-ASSISTED RADICAL PROSTATECTOMY: IMPACT ON THE NUMBER OF NODES AND ON NODAL INVASIO
Quantum dots nanoparticle-based lateral flow assay for rapid detection of Mycobacterium species using anti-FprA antibodies.
No full textA lateral flow (LF) device combined with
quantum dots (QDs) technology was developed
for rapid detection of a specific mycobacterial
flavoprotein reductase (fprA). In order to
develop the LF assay based on a double-antibody
sandwich format, two monoclonal antibodies
recognizing different epitopes located
in separated fprA domains were identified. The
first monoclonal antibody was immobilized
onto the detection zone of a porous nitrocellulose
membrane, whereas another monoclonal
antibody was conjugated to QDs nanoparticles
as a detection system. Using these monoclonal
antibodies we recorded a good fluorescence
signal, the intensity of which was directly proportional
to the concentration of fprA protein.
The use of antibodies conjugated with fluorescent
semiconductor QDs via biotin-streptavidin
bridge, allowed the detection of fprA protein
at concentrations as low as 12.5 pg/μL in
less than 10 min. The reported technology
could be useful in the diagnostic investigation
of Mycobacterium tuberculosis and other
human pathogens in clinical specimen
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
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