6,689 research outputs found
As relações entre a disciplina Laboratorio di Sintesi Finale do Politecnico di Milano e o ensino de projeto em moda no Brasil.
No full textA partir da experiência no Politecnico di Milano, acompanhando a disciplina Laboratorio di Sintesi Finale, de responsabilidade do professor Giovanni Conti, foi possível registrar as etapas que configuram um processo de projetação em moda e certa ordem para que elas ocorram. Durante esse período, foi possível observar com que frequência é utilizado o vocabulário e práticas do design industrial que, por vezes, é flexibilizado em função das necessidades específicas do design de moda. Além de frequentar as aulas de Conti, foi realizada uma entrevista de caráter exploratório com o docente, visando a coleta de dados primários mais precisos que, posteriormente foram tratados respeitando os métodos da História Oral para a construção de relatos, como aqueles de Alberti (2013), Poupart (2008) e Velho (1980). Dessa forma, a problematização do tema produzida como resultado dessa prática de pesquisa permitiu chegar a reflexões que expõem a importância da prática do design de moda e sua institucionalização para o avanço do campo
The radical right in Europe, between slogans and voting behavior. IHS Political Science Series No. 123, July 2011
No full textThe paper analyzes the radical right‘s attitudes toward the EU focusing in particular on the level of congruence between the programmatic statements of the central office and the voting behavior of their MEPs. It shows that although radical right parties represent a source of opposition to the EU, within the EP they express their dissent making use of the rules of the game, voting with the opposition more than the other forces do, but voting almost as much with the majority. The party public office in the EP is inserted in the legislative process and even more collusive with the other parties of both sides of the political spectrum than the Eurosceptical rhetoric and statements of central office makes the public believe
Palabra siliente : un oxímoron dilecto (a propósito de los textos de J. L. Borges)
No full textFil: Conti, Enrique.
Consejo Nacional de Investigaciones Científicas y Técnica
Design, synthesis and biological characterization of PfGAPDH inhibitors
No full textMalaria has a tremendous health, social and economic impact on people living in tropical and subtropical regions of the world. WHO estimated 214 million cases and 438 000 deaths in 2015 and 3.2 billion people are globally at risk of infection1. Available treatments are becoming progressively less effective, mainly because parasites are constantly acquiring resistance toward the drug in use. Therefore, there is an urgent need of developing therapeutic agents acting on new targets.
Plasmodium parasites (P. falciparum is the most lethal), in their amastigote phase, produce energy only through glycolysis. In this pathway, GAPDH catalyses the first energy productive step and thus represent a valuable target for drug discovery. Known GAPDH inhibitors that bind to the active site are small nonselective molecules (iodoacetamide, 3-bromopyruvate), which irreversibly react with cysteine residues of the enzyme, including the catalytic one. We designed and synthesized the first series of inhibitors characterized by a 3-bromoisoxazoline warhead2, inspired by 3-bromoacivicin, a known inhibitor of reactive cysteine containing enzymes3. The compounds were assayed for the inhibitory activity on the P. falciparum isolated enzyme showing a biphasic irreversible inactivation. MS/MS studies of the digested protein demonstrated the mild reactivity of the exploited warhead, which only reacts with catalytic Cys (activated by a His residues). The human ortholog of GAPDH is only partially inhibited (up to 25%). We explained this through a negative cooperativity that prevents the alkylation of all the four monomers of hGAPDH, resulting in selectivity among the two isoforms of GAPDH4. Compounds were also assayed on cultures of the bloodstream form of P. falciparum and human cell lines in order to determine their in vitro antiparasitic activity and toxicity.
The goal of this project is to obtain compounds with improved potency and pharmacokinetic properties, suitable for in vivo studies on animal model of P. falciparum infections.
1. http://www.who.int/mediacentre/factsheets/fs094/en/
2. Bruno, S.; Pinto, A.; Paredi, G.; Tamborini, L.; De Micheli, C.; La Petra, V.; Marinelli, L.; Novellino, E.; Conti, P.; Mozzarelli, A. J. Med. Chem. 2014, 57(17), 7465-7471.
3. a) Conti, P.; Pinto, A.; Wong, P.E.; Major, L.L.; Tamborini, L.; Iannuzzi M.C.; De Micheli, C.; Barrett, M.P.; Smith, T.K. ChemMedChem 2011, 6(2), 329-333. b) Tamborini, L.; Pinto, A.; Smith, T.K.; Major, L.L.; Iannuzzi, M.C.; Cosconati, S.; Marinelli, L.; Novellino, E.; Lo Presti, L.; Wong, P.E.; Barrett, M.P.; De Micheli, C.; Conti, P. ChemMedChem 2012, 7(9), 1623-1634.
4. Bruno, S.; Margiotta, M.; Pinto, A.; Cullia, G.; Conti, P.; De Micheli, C.; Mozzarelli, A. Bioorg. Med. Chem. 2016, 24(12), 2654-2659
Regulación hormonal del epitelio uterino del conejo
No full textFil: Conti, Claudio Jorge. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Regulación hormonal del epitelio uterino del conejo
No full textFil: Conti, Claudio Jorge. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Algae as biomarkers, bioaccumulators and toxin producers
No full textUncontrolled accumulation of wastes in marine environments involves an increase in organic substances and/or nutrients, as well as toxicity. The consequences of this enrichment for the homeostasis of ecosystems are unpredictable. The best studied consequence of such enrichment is eutrophication (Conti, 1996). Natural or anthropogenic eutrophication is the enrichment of a water body in nutrients, utilizable by algae. Sources of these nutrients are the atmosphere, rivers, soil erosion and runoff from catchment areas, sewage, industrial effluents, fertilizers, and waste disposal from animal farms. Mathematical models predicting pollutant dispersion and pollutant bioavaibility may make a substantial contribution to the study of the marine pollution phenomena and may be a valuable tool for the description of the pollution flux (Benedini and Cicioni, 1992). Generally, mathematical models refer to the propagation and transport of particular pollutants originating from one-point contamination sources and also in this case need a broad spectrum of experimental data. This requirement is particularly true for sea ecosystems, owing to the large number of required variables and to the various effects on different species and ecosystems. For these reasons, biomonitoring programmes in sea environments are very important. Establishing the pollutant level in organisms may help to evaluate the possible toxicity for various species at different levels of the trophic chain
GLT-1 promoter activity in astrocytes and neurons of mouse hippocampus and somatic sensory cortex
No full textGLT-1 eGFP BAC reporter transgenic adult mice were used to detect GLT-1 gene expression in individual cells of CA1, CA3 and SI, and eGFP fl uorescence was measured to analyze quantitatively GLT-1 promoter activity in different cells of neocortex and hippocampus. Virtually all GFAP+ astrocytes were eGFP+; we also found that about 80% of neurons in CA3 pyramidal layer, 10-70% of neurons in I-VI layers of SI and rare neurons in all strata of CA1 and in strata oriens and radiatum of CA3 were eGFP+. Analysis of eGFP intensity showed that astrocytes had a higher GLT-1 promoter activity in SI than in CA1 and CA3, and that neurons had the highest levels of GLT-1 promoter activity in CA3 stratum pyramidale and in layer VI of SI. Finally, we observed that the intensity of GLT-1 promoter activity in neurons is 1-20% of that measured in astrocytes. These results showed that in the hippocampus and neocortex GLT-1 promoter activity is observed in astrocytes and neurons, detailed the distribution of GLT-1 expressing neurons, and indicated that GLT-1 promoter activity in both astrocytes and neurons varies in different brain regions. © 2010 de Vivo, Melone, Rothstein and Conti
The geological map of Sardinia (Italy) at 1:250,000 scale
No full textOver the last 25 years the Italian national geological mapping program of the Italian Geological Survey (CARG Project, italian: Progetto Carta Geologica) at 1:50,000 scale has led to significant improvements in the geological knowledge for the Island of Sardinia (Italy). As a result, about one half of the island now is covered by new geological maps with 1:10,000–1:25,000 accuracy and geological maps at the 1:50,000 scale whose explanatory notes are available electronically. At the beginning of the CARG Project a geological map for Sardinia Island at 1:200,000 scale was published [Carmignani, L. (1996). Carta Geologica della Sardegna (1:200.000). Servizio Geologico Nazionale, Regione Autonoma della Sardegna], summarizing all the geological information available at that time, and a book with explanatory notes for the map was later published [Carmignani, L., Oggiano, G., Barca, S., Conti, P., Salvadori, I., Eltrudis, ... Pasci, S. (2001). Geologia della Sardegna: Note Illustrative della Carta Geologica della Sardegna in scala 1:200.000, Memorie Descrittive della Carta Geologica d'Italia (Vol. 60). Roma: Servizio Geologico d'Italia, 283 pp]. The enclosed Geological map of Sardinia at 1:250,000 scale incorporates all maps of the CARG Project, unpublished author studies and recently published maps and represents the most updated synthesis of an area characterised by a complex geological evolution that, with few exceptions, can be considered continuous during the last 540 Ma. The main events that influenced the geology of the island are the Variscan orogen that deeply involved the passive margin of North Gondwana and then the complex episodes that occurred in the present-day Mediterranean area after the accretion of Pangea up to the opening of the Tyrrhenian basin
A highly efficient flow reactor process for the synthesis of N-Boc-3,4-dehydro-L-proline methyl ester
No full textThe multi-step preparation of N-Boc-3,4-dehydro-L-proline methyl ester using a modular flow reactor is
reported. The use of immobilised reagents and scavengers in pre-packed glass tubes allows us to obtain the pure product in 87% overall yield, 97% purity, and >98% enantiomeric excess without any additional purification step. Our flow-based protocol enables the rapid multi-gram synthesis (about 9 g/12 h) of the desired product
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