1,721,039 research outputs found
L’integrazione politica ed economica dei migranti, tra ordinamento interno e prospettive comunitarie: alcuni spunti di riflessione
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Characterization of a Salmonella Transcription Factor-DNA Complex and Identification of the Inducer by Native Mass Spectrometry
No full textFraR, a transcriptional repressor, was postulated to regulate the metabolism of the Amadori compound fructose-asparagine (F-Asn) in the foodborne pathogen Salmonella enterica. Here, the DNA- and inducer-binding affinities and stoichiometries of FraR were determined and cross-validated by electrophoretic mobility-shift assays (EMSAs) and online buffer exchange coupled to native mass spectrometry (OBE-nMS). We demonstrate the utility of OBE-nMS to characterize protein and protein-DNA complexes that are not amenable to offline exchange into volatile buffers. OBE-nMS complemented EMSAs by revealing that FraR binds to the operator DNA as a dimer and by establishing 6-phosphofructose-aspartate as the inducer that weakens DNA binding by FraR. These results provide insights into how FraR regulates the expression of F-Asn-catabolizing enzymes and add to our understanding of the intricate bacterial circuitry that dictates utilization of diverse nutrients
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Characterization of Transcription Factor-DNA Complexes Using Online Buffer Exchange Coupled to Native Mass Spectrometry
No full textProduction of PEA composite microparticles with polyvinylpyrrolidone and luteolin using Supercritical Assisted Atomization
No full textPalmitoylethanolamide (PEA) has anti-inflammatory, pain controlling and neuroprotective properties, but its use is limited by its low water solubility and corresponding bioavailability. In this work, Supercritical Assisted Atomization (SAA) coprecipitation is used to reduce PEA tendency to crystallize, using polyvinylpyrrolidone (PVP) and luteolin (LUT) and operating at different process parameters. Spherical microparticles were produced at PEA/PVP ratio 1:8 wt/wt in ethanol, with D50 of 400 nm and with the best result as particle morphology. X-ray analysis reveals that these PEA-PVP particles are amorphous and PEA loading efficiency reaches 100%. In the case of PEA-LUT coprecipitates, very small quantities of LUT stabilized the microparticles, inhibiting PEA crystallization process. Indeed, quasi spherical microparticles were produced at 10:1 wt/wt PEA/LUT ratio, with D50 of 970 nm and PEA loading efficiency over 99%. To explain how very small quantities of LUT can modify PEA crystallization behavior, specific PEA-LUT interactions were evidenced by FT-IR analysis
Elucidating the catalytic mechanism of a bacterial deglycase essential for utilization of fructose-lysine, an Amadori product
No full textSynthesis of trifluoromethyl-substituted pyrazolo[4,3-c]pyridines - Sequential versus multicomponent reaction approach
No full textA straightforward synthesis of 6-substituted 1-phenyl-3-trifluoromethyl-1H- pyrazolo[4,3-c]pyridines and the corresponding 5-oxides is presented. Hence, microwave-assisted treatment of 5-chloro-1-phenyl-3-trifluoromethylpyrazole-4- carbaldehyde with various terminal alkynes in the presence of tert-butylamine under Sonogashira-type cross-coupling conditions affords the former title compounds in a one-pot multicomponent procedure. Oximes derived from (intermediate) 5-alkynyl-1-phenyl-3-trifluoromethyl- 1H-pyrazole-4-carbaldehydes were transformed into the corresponding 1H-pyrazolo[4,3-c]pyridine 5-oxides by silver triflate-catalyzed cyclization. Detailed NMR spectroscopic investigations (1H, 13C, 15N and 19F) were undertaken with all obtained products. © 2014 Palka et al; licensee Beilstein-Institut
Anatomy and kinematic evolution of an ancient passive margin involved into an orogenic wedge (Western Southern Alps, Varese area, Italy and Switzerland)
Full text linkWe make use of own geological mapping, interpretations of seismic reflection profiles and deep geophysical data to build a lithospheric-scale cross-section across the European Western Southern Alps (Varese area) and to model a progressive restoration from the end of Mesozoic rifting to present-day. Early phases of Alpine orogeny were characterized by Europe-directed thrusting, whereas post-Oligocene shortening led to basement-involving crustal accretion accompanied by backfolding, and consistent with the kinematics of the adjoining Ivrea Zone. Wedging was favored by a significant component of reactivation of the inherited Adriatic rifted margin. Our results also suggest that, during the collisional and post-collisional tectonics, lithosphere dynamics drove diachronically the onset of tectonic phases (i.e., wedging and slab retreat), from east to west, across the Western Southern Alps
EFFICACIA DI UNA NEUROPOROTESI BASATA SULLA STIMOLAZIONE ELETTRICA FUNZIONALE PER IL PIEDE CADENTE NEL RECUPERO DELLA DEAMBULAZIONE: STUDIO PILOTA RANDOMIZZATO E CONTROLLATO NEI PAZIENTI CON ICTUS IN FASE SUBACUTA
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