1,721,060 research outputs found
Investigation of 74 microhaplotypes for kinship testing in US populations
No full textMicrohaplotypes are markers that consist of haplotype blocks of SNPs, which can be analyzed by massively parallel sequencing technologies. These allow determining the haplotype phase at every locus by clonal sequencing each DNA strand. MHs are polymorphic loci with same size alleles, no stutter, and lower mutation rate than STRs. They can provide the same power of discrimination of STR-kits, thus useful for mixture deconvolution, but more accurate ancestry prediction than STRs. In this study we investigated the potential of a recently developed 74plex-MH panel for kinship testing using the Familias software.
Samples from families of four major US population groups were collected and genotyped using the 74plex-MH panel. MH allele frequency data from 347 individuals were imported into Familias software along with STR allele frequency data of 29 loci (NIST dataset) from 1036 individuals. Different family scenarios were tested and these included unrelated vs parent-child, unrelated vs full siblings, unrelated vs half siblings, unrelated vs cousin pairs. The prediction of the kinship relation for the four populations of interest was reported as Log10 of the likelihood ratio (LR).
Overall, the panel of 74MHs and 29STRs showed similar performance in predicting the correct kinship scenarios tested. Correct prediction was reported for parent-child, full siblings, and half sibling scenarios, but not for the cousin pairs scenario. The panel of 74 MHs showed larger Log10LR values than the 29 STR-assay, thus demonstrating the effectiveness of this biomarker as a tool for kinship testing in addition to mixture deconvolution and ancestry prediction
Innovative heterotrophic autotrophic denitrification for drinking water: effect of ZVI on nitrate removal
No full textSite visit: esercizio on site visit 2010-2011: risultati del terzo esercizio, primo ciclo
No full textThe third experience of the first series of the Site
Visit CPPCLMMCC project is now concluded.
The results concerning both filled questionnaires
analysis and on site visit reports are briefly
discussed. The main project goals appear to be
reached in terms of common problems analysis,
excellences checking and divulgation, quality
control stimulus, and homogenization in teaching
objectives and organization. It’s now
time to develop a peer review and accreditation
system, officially recognized, based on shared
minimal requirements for qualificatio
Heterotrophic/autotrophic denitrification (HAD) of drinking water: prospective use for permeable reactive barrier
No full textAn heterotrophic/autotrophic denitrification (HAD) approach for nitrate removal from drinking water
No full text
Application of photocatalysis as a post treatment method of a heterotrophic–autotrophic denitrification reactor effluent.
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