5 research outputs found
www.omicsonline.com Research Article Computational Annotation for Hypothetical Proteins of Mycobacterium Tuberculosis
No full textCopyright: © 2008 Anandakumar S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. There is rising death of humans worldwide by reason of tuberculosis. The current sequencing of the Mycobacterium tuberculosis genome holds assure for the development of new vaccines and the design of new drugs. In this view, the functions prediction of genomic sequences for hypothetical proteins will invigorate our knowledge with reference to the identification of new drugs for tuberculosis. There are various function prediction methods available based on the on the assumption. The process accurate annotation for genes in newly sequenced genomes currently has been based on sequence similarity. In this work about 250 hypothetical proteins of Mycobacterium tuberculosis taken functions were predicted using Bioinformatics web tools, BLAST, INTERPROSCAN, PFAM and COGs
Estudo da atividade antitumoral do extrato bruto e frações de Casearia sylvestris
No full textDissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências Biológicas, Programa de Pós-Graduação em Bioquímica, Florianópolis, 2010O desenvolvimento de novas alternativas terapêuticas, como as terapias que utilizam as plantas medicinais, tem despertado grande interesse no tratamento do câncer, pois as terapias disponíveis não são capazes de regredir totalmente a evolução da patologia e/ou apresentam elevada toxicidade. A C. sylvestris, planta medicinal que possui vários usos populares no Brasil e outros paises americanos, tem sido indicada para o tratamento de tumores. Nesse contexto, o presente trabalho propôs avaliar as atividades citotóxica, antiproliferativa, pró-apoptótica, antiangiogênica e antitumoral do extrato bruto (EB-ETOH), e das frações metanólica (f-MeOH), acetato de etila (f-AcOEt) e clorofórmica (f-CHCl3) de Casearia sylvestris utilizando modelos experimentais in vitro e in vivo. Nos ensaios do MTT e incorporação de timidina triciada realizados respectivamente, para investigar a citotoxicidade e influência dos extratos sobre a proliferação celular, os resultados demonstraram que EB-ETOH, f-CHCl3 e f-AcOEt reduziram de maneira significativa a viabilidade e proliferação de células do carcinoma de Ehrlich, sendo a citotoxicidade promovida pelo tratamento com esses extratos, tempo e concentração dependente. Diante do efeito citotóxico promovido por EB-ETOH, f-CHCl3 e f-AcOEt sobre células do carcinoma de Ehrlich, avaliou-se o tipo de morte celular promovida pelos tratamentos. A coloração com brometo de etídeo/laranja de acridina (BE/LA) revelou que o provável tipo de morte celular induzida pelos tratamentos trata-se de apoptose, uma vez que a grande maioria das células, após terem entrado em contato com a referida solução corante, adquiriram uma coloração laranja-avermelhada, característica da ocorrência do fenômeno apoptótico. Entretanto, em relação à avaliação da atividade nucleásica, verificada através da realização do teste de dano ao DNA plasmidial, observou-se que os efeitos antiproliferativo e citotóxico induzidos por EB-ETOH, f-CHCl3 e f-AcOEt, não são mediados por uma interação e dano direto ao DNA. Além disso, a realização desse ensaio permitiu observar que os extratos têm a capacidade de proteger o DNA do ataque induzido por EROs, excluindo-se portanto, a possibilidade de estarem exercendo seus efeitos citotóxico e antiproliferativo através da indução de estresse oxidativo no núcleo. As avaliações morfológicas e bioquímicas realizadas nas amostras obtidas de camundongos portadores do tumor ascítico de Ehrlich mostraram que os animais tratados com EB-ETOH e f-CHCl3 apresentaram menor ganho de peso corporal (26 e 31%, respectivamente) e de circunferência abdominal (52 e 58%, respectivamente), menor volume de líquido ascítico (46 e 43%, respectivamente), aumento do percentual de longevidade (48 e 40%, respectivamente) e da proporção de células inviáveis/viáveis (70 e 78%, respectivamente), quando comparados ao grupo controle negativo. Com a realização do modelo in vivo do TAE, observou-se ainda que o efeito antitumoral promovido por esses extratos é dose-dependente, uma vez que se apresentou-maior, quanto mais elevada a concentração de extrato administrada ao animal. A realização do ensaio do CAM, permitiu verificar que tanto o EB-ETOH, como f-CHCl3 de C. sylvetris, exercem um significativo efeito antiangiogênico, de maneira dose-dependente, onde a administração de 3,25; 7,5 e 15 µg/disco de EB-ETOH e f-CHCl3 foram capazes de diminuir o percentual de vasos, em torno de 54,5; 61,4 e 71% e 42,1; 59,1 e 70,9%, respectivamente. Por fim, cabe ressaltar que a realização da biometria dos embriões possibilitou observar que o tratamento dos mesmos com extrato bruto e fração clorofórmica não se apresentou tóxico, uma vez que não alterou o desenvolvimento embrionário nem a morfogênese externa, avaliados pelo comprimento total do embrião, razão comprimento cefálico/comprimento truncal e razão comprimento cefálico/comprimento total. Dessa forma, conclui-se que EB-ETOH e f-CHCl3 de C. sylvestris apresentaram efeito citotóxico, antiproliferativo, pró apoptótico e antitumoral in vitro e in vivo, sobre as células do carcinoma de Ehrlich. Tal efeito antitumoral mediado por esses extratos, não envolvem sua intercalação ao DNA, e sugere-se que se deva, pelo menos em parte, à inibição da formação de vasos e a conseqüente supressão nutricional das células tumorais
Individual gendered experiences of organisational elements impacting on knowledge creation processes in a knowledge-intensive organisation
No full textThis thesis explores individual gendered experiences of organisational elements impacting on knowledge creation processes of management consultants in an international management consultancy. There have been calls to gain further insights into knowledge creation by exploring the impact of social aspects such as work practices, participation and organisational conflict on knowing and learning processes. These calls have been addressed mainly by considering single aspects of the organisational context and their impact on knowledge creation processes or by considering a range of different aspects of the organisational context but neglecting their interrelationship. This current research explores management
consultants’ experiences of various social aspects, which are understood as organisational elements, impacting on knowledge creation processes and the interrelationships between these aspects.
According to the social-constructionist perspective on knowledge and learning, social interaction is integral to knowing and learning. Since gender is understood to impact on social interaction, in this thesis it is acknowledged that knowledge creation, which is inherent to knowing and learning processes, is influenced by gender. Previous research tended to neglect the impact of gender on individual experiences of knowledge creation processes. More recently, women’s inclusion and exclusion from knowledge creation processes in organisations has been explored through a theoretical analysis of a single organisational aspect, knowledge creation through networking.
Drawing upon a social-constructionist perspective on knowing and learning and gender in organisations, this thesis contributes to theory in the area of knowledge creation and gender in organisations by placing special emphasis on the role of gender whilst exploring various key aspects of the organisational context impacting on individual experiences of knowledge creation processes.
The theoretical potential of this research is developed through an exploratory case study of 15 men and women consultants working for the case study organisation. Through semi-structured interviews, accounts of individual gendered experiences of organisational elements impacting on knowledge creation processes were explored. Here, women, who have been neglected in previous research, received the same attention as men. New insights are offered by not only exploring women’s experiences and the potential differences between women’s and men’s experiences but also considering the differences within the women’s and men’s accounts. Following an autoethnographic approach this research also offers a view from the ‘inside’ by including the researcher’s own experiences as an insider management consultant, thus offering a further contribution.
This thesis argues that career opportunities, individual acknowledgement within the organisational context, motivation and trustful relationships are key aspects impacting on knowledge creation experiences of women and men management consultants. These aspects are interlinked and impact on each other. The research offers career opportunities and individual acknowledgement as key influences to the field of knowledge creation. Further, it illustrates how individuals’ experiences of
organisational elements that impact on knowledge creation processes in a knowledge-intensive organisation are gendered
Leader and follower perspectives of entrepreneurial leadership: how is gender experienced in small firms?
No full textThis study aims to bring a gender perspective to the study of entrepreneurship and followers' perspectives of entrepreneurial leadership. The study contributes to emerging entrepreneurial leadership research considering gender, acknowledging follower involvement, individual agency, and recognising entrepreneurial leadership as a social process.
Secondly, the study contributes further empirical research from a gender perspective of women entrepreneurs to the authentic leadership theory base
