1,720,964 research outputs found
Functional studies of PRP3 pre-RNA splicing factor mutated in patients affected by retinitis pigmentosa.
No full textPurpose: Many genes mutated in retinitis pigmentosa (RP) are components of the phototransduction cascade. However, in the last 2 years, genes encoding pre–RNA splicing factors were found mutated in three forms of autosomal dominant RP (adRP, RP11, RP13 and RP18). The slicing factors involved in adRP are PRPF31, PRP3, PRPF8. The link between mutant splicing factors and retinal degeneration is completely obscure. Methods: RNA in situ hybridization studies for the analysis of the expression pattern of PRP3 in embryonic and adult eyes. Subcellular localization of the protein using transfection experiment. Subcellular localization of the mutant forms of Prp3 as found in patients. Results: We analyzed expression of the Prp3 gene in the developing and adult murine eye by RNA in situ hybridization. We found that Prp3 transcripts are ubiquitously distributed but higher amounts could be detected in the central nervous system and neural retina. Expression does not seem to be strictly correlated with the photoreceptor cell layer. Using in vitro transfection in HeLa, NIH3T3 and Cos7 cells, Prp3 localizes into the nuclei. Using similar approaches we found the two different mutations (P493S and T494M) have similar intracellular distribution as found for the wild–type protein. We then wanted to study Prp3 protein distribution compared to splicing proteins. Co–localization analysis of transfected cells using antibodies recognizing a protein of the splicing complex suggests that Prp3 only partially co–localizes with the spliceosome. Conclusions: We analyzed Prp3 transcript distribution in vivo and found no direct correlation with photoreceptors, the cells that undergo degeneration in patients with mutations in this gene. Co–localization with splicing factors confirms that the protein is part of the spliceosome. However the protein shows a more complex subcellular distribution suggesting additional roles of this protein. Finally, mutant Prp3 is translocated into the nucleus like the wild type, ruling out the possibility of mis–folding and degradation of the mutant protein
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
- …
