447 research outputs found
О содержании провитаминов D и холестерина у донных беспозвоночных Черного моря
No full textИсследовали содержание провитаминов D и стеринов холестеринового ряда у актиний - Actinia aequina, многощетанковых червей - Nereis diversicolor, десятиногих раков - Leander adspersus, равноногих раков - Idothea baltica, бокоплавов - Gammarus locusta, моллюсков - Ostrea taurica Kryn., а для сравнения - у представителя хордовых - асцидий Molgula euprocta. У всех изученных видов стерины представлены в основном холестерином. Особенно высокое содержание холестерина отмечено у актинии Actinia aequina и у полихеты Nereis diversicolor. У членистоногих с усложнением их организации количественное содержание стеринов возрастает, достигая максимального значения у десятиногих раков - Leander adspersus. Высокое содержание стеринов отмечено также и у Ostrea taurica Kryn
R&D proposal: the prism plastic calorimeter:PPC
No full textThis proposal supports two goals: First Goal_Demonstrate that current, widely used plastic technologies allow to design Prism Plastic Calorimeter (PPC) towers with a new "liquid crystal" type plastic called Vectra. It will be shown that this technique meets the requirements for a LHC calorimeter with warm liquids: safety, hermeticity, hadronic compensation, resolution and time response. Second Goal_ Describe how one can design a warm liquid calorimeter integrated into a LHC detector,and list the advantages of the PPC: low price, minimum of mechanical structures, minimum amount of dead space, easiness of mechanical assembly, accessibility to the electronics, possibility to recirculate the liquid. The absorber and the electronics being outside the liquid and easily accessible, one has maximum flexibility to define them. The R&D program we define here aims at showing the feasibility of these new ideas by building nine towers of twenty gaps and exposing them to electron and hadron beams
Organic liquid TPCs for neutrino physics
No full textInternational audienceWe present a new concept for anti-neutrino detection, an organic liquid TPC with a volume of the order of m3 and an energy resolution of the order of 1% at 3 MeV and a sub-cm spatial resolution
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Search for solar axions produced in the p(d,3He)A reaction with Borexino detector
No full text11 pages, 7 figures, submitted to Phys.Rev.DA search for 5.5 MeV solar axions produced in the p+d→3He+A (5.5 MeV) reaction was performed using the Borexino detector. The Compton conversion of axions to photons, A+e→e+γ; the axioelectric effect, A+e+Z→e+Z; the decay of axions into two photons, A→2γ; and inverse Primakoff conversion on nuclei, A+Z→γ+Z, are considered. Model-independent limits on axion-electron (gAe), axion-photon (gAγ), and isovector axion-nucleon (g3AN) couplings are obtained: |gAe×g3AN|≤5.5×10-13 and |gAγ×g3AN|≤4.6×10-11 GeV-1 at mA<1 MeV (90% confidence level). These limits are 2-4 orders of magnitude stronger than those obtained in previous laboratory-based experiments using nuclear reactors and accelerators
Overexpression of the Notch1 intracellular domain using the <i>dusp6:Gal4-ERT</i> driver disrupts notochord development.
No full text<p>(<b>A and B</b>) EGFP expression at the 10-somite stage in control (A) and 2 μM 4-OHT treated <i>Tg(dusp6:Gal4-ERT-VP16; UAS:EGFP)</i> fish. The green arrow points to EGFP expression at the midline. (<b>C–F</b>) DIC images of control (C and D) and 4-OHT treated (4 μM from 2–24 hpf, E and F) <i>Tg(dusp6:Gal4-ERT-VP16; UAS:NICD)</i> animals at 24 hpf. Regions bounded by the dashed red box in panels C and E are shown in high magnification in panels D and F, respectively. In panel D, the red arrow indicates the floor plate (fp) and the blue arrow indicates the notochord (nc); in panel E, the magenta arrow highlights the reduced notochord and disorganized floor plate.</p
The Gal4-ERT system provides rapid induction of gene expression.
No full text<p>(<b>A–D</b>) Kinetics of EGFP expression in <i>Tg(krt5:Gal4-ERT-VP16; UAS:EGFP)</i> animals upon administration of 2 μM 4-OHT at 24 hpf for 0–4.5 hours. (<b>A′–D′</b>) High magnification images demonstrating EGFP expression for each treatment. White arrowheads point to epidermal expression of EGFP; blue arrows indicate bleed-through from the heart muscle-specific ECFP transgenesis marker.</p
Inducible transgene expression in adult zebrafish.
No full text<p>(<b>A–D</b>) Expression of EGFP in the eye of a single adult <i>Tg(krt5:Gal4-ERT-VP16; UAS:EGFP)</i> zebrafish prior to (A and B) and after (C and D) treatment with 1 μM tamoxifen for one hour per day for three consecutive days. Green arrows point to EGFP expression in the eye. The areas bounded by the dashed red box in A and C are shown at high magnification in B and D, respectively. (<b>E–H</b>) Immunostaining of paraffin sections with anti-EGFP antibodies (shown in green) in eyes from DMSO- (E and F) and tamoxifen-treated (G and H) <i>Tg(krt5:Gal4-ERT-VP16; UAS:EGFP)</i> fish. Panels F and H show overlays with anti-EGFP antibody staining in green, Hoechst-stained nuclei in blue, and auto-fluorescence in red. Fish were drug treated as in A–D. Green arrows indicate EGFP expression in photoreceptors and asterisks (*) denote auto-fluorescence in photoreceptor outer segments. (<b>I</b>) In situ hybridization for <i>EGFP</i> mRNA in a paraffin section from a 4-OHT treated <i>Tg(krt5:Gal4-ERT-VP16; UAS:EGFP)</i> animal. The blue arrow shows <i>EGFP</i> expression in photoreceptors.</p
Transgene expression levels depend upon 4-OHT dosage.
No full text<p>(<b>A–D</b>) EGFP expression upon treatment of <i>Tg(krt5:Gal4-ERT-VP16; UAS:EGFP)</i> zebrafish with ethanol or the indicated dose of 4-OHT from 4–24 hpf. The blue arrow indicates <i>myl7:ECFP</i> expression. (<b>A′–D′</b>) High-magnification images of ventral epidermis from fish in each treatment group. (<b>E</b>) Normalized EGFP intensity (to the 0.5 μM 4-OHT treated group) of fish treated with ethanol or 4-OHT. Error bars represent standard deviations.</p
Temporally and spatially controlled transgene expression in zebrafish.
No full text<p>(<b>A</b>) Schematic of transgenic constructs used in the Gal4-ERT system. A tamoxifen-responsive Gal4-ERT-VP16 construct is expressed from a tissue-specific promoter that activates any UAS-linked responder line (shown here as a <i>5xUAS:EGFP</i> reporter) upon tamoxifen or 4-OHT exposure (orange squares). The <i>myl7:ECFP</i> cassette serves as a transgenesis marker for the Gal4-ERT lines. (<b>B–D</b>) Visualization of EGFP expression in <i>Tg(krt5:Gal4-ERT-VP16; UAS:EGFP)</i> animals treated with ethanol (B) or 2 μM 4-OHT (C and D) from 4–24 hpf. The white arrow in panel D highlights expression of EGFP in the epidermis. (<b>E–I</b>) EGFP expression in <i>Tg(dusp6:Gal4-ERT-VP16; UAS:EGFP)</i> animals treated with vehicle (E) or 2 μM 4-OHT (F-I) from 4–24 hpf. In panels G and H, the white arrow indicates EGFP expression in the hindbrain and midbrain-hindbrain boundary. In panel I, arrowheads mark dorsal spinal cord neurons and the arrow points to EGFP expression in the floor plate. (<b>J–M</b>) Expression of EGFP in control (J) and 4-OHT treated (2 μM, K-M) <i>Tg(ef1α:Gal4-ERT-VP16; UAS:EGFP)</i> animals in a variety of cell types throughout the embryo including skeletal muscle (K, white arrow), the eye (L, white arrow), and the midbrain/midbrain-hindbrain boundary (M, white arrow). In panels B, E, and J, blue arrows point to myocardial ECFP expression, which represents the marker for transgenesis and serves as an internal control.</p
Using inducible expression to define temporal roles of Notch1 signaling in notochord development.
No full text<p>(<b>A–E</b>) Expression of myc-tagged NICD by immunostaining with myc antibodies on 24 hpf <i>Tg(dusp6:Gal4-ERT-VP16; UAS:NICD)</i> embryos treated with ethanol (A) or 4 μM 4-OHT at the indicated times (B–E). Blue arrows indicate myocardial ECFP expression to mark transgenic animals and magenta arrows show expression of myc-tagged NICD. Panel insets display high magnification images of boxed regions where red arrows indicate the notochord (A and E) and the missing notochord (B–D). (<b>F</b>) Normalized penetrance of notochord defects in <i>Tg(dusp6:Gal4-ERT-VP16; UAS:NICD)</i> animals treated with 4-OHT at the indicated stages of development. Each data point represents the normalized fraction of affected animals in sets of treated embryos from four independent clutches. The data is normalized to the average fraction of abnormal fish in the four 4–24 h sets. The double asterisk indicates a significant difference between 4–24 h and 10–24 h 4-OHT treated fish (P<0.005). (<b>G–I</b>) Expression of <i>shha</i> in the floor plate of animals treated with ethanol (G) or with 4-OHT for the indicated times (H–I). Boxed regions are shown at higher magnification in the panel insets. Red arrows indicate <i>shha</i> expressing floor plate cells.</p
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