2,049 research outputs found
Should we use early less invasive hemodynamic monitoring in unstable ICU patients?
No full textIn the previous issue of Critical Care, Takala and colleagues presented the results of a multicenter study to investigate whether the early presence of less invasive hemodynamic monitoring improves outcome in patients admitted with hemodynamic instability to the intensive care unit. The authors' results suggest that it makes no difference. We discuss these findings and compare them to the literature on early goal-directed therapy in which monitors are used early but with a protocol
Proteomic analysis of pancreatic endocrine tumor cell lines treated with the histone deacetylase inhibitor trichostatin A
No full textEffects of the histone-deacetylases inhibitor trichostatin A (TSA) on the growth of three different
human pancreatic endocrine carcinoma cell lines (CM, BON, and QGP-1) have been assessed via
dosage-dependent growth inhibition curves. TSAdetermined strong inhibition of cell growth with
similar IC50 values for the different cell lines: 80.5 nM(CM), 61.6 nM(BON), and 86 nM(QGP-1),
by arresting the cell cycle in G2/M phase and inducing apoptosis. 2DE and nano-RP-HPLC-ESIMS/
MS analysis revealed 34, 33, and 38 unique proteins differentially expressed after TSA treatment
in the CM, BON, and QGP-1 cell lines, respectively. The most important groups of modulated
proteins belong to cell proliferation, cell cycle, and apoptosis classes (such as peroxiredoxins 1
and 2, the diablo protein, and HSP27). Other proteins pertain to processes such as regulation of
gene expression (nucleophosmin, oncoprotein dek), signal transduction (calcium-calmodulin),
chromatin, and cytoskeleton organization (calgizzarin, dynein, and lamin), RNA splicing
(nucleolin, HNRPC), and protein folding (HSP70). The present data are in agreement with previous
proteomic analyses performed on pancreatic ductal carcinoma cell lines (Cecconi, D. et al.,
Electrophoresis 2003; Cecconi, D. et al., J. Proteome Res. 2005) and place histone-deacetylases inhibitors
among the potentially most powerful drugs for the treatment of pancreatic tumor
Variation of Saturn's UV aurora with SKR phase
No full textpeer reviewedIt is well known that a wide range of kronian magnetospheric phenomena, including the Saturn kilometric radiation (SKR), exhibit oscillations near the planetary rotation period. However, although the SKR is believed to be generated by unstable auroral electrons, no connection has been established to date between diurnal SKR modulations and UV auroral power. We use an empirical SKR phase determined from Cassini observations to order the 'quiet time' total emitted UV auroral power as observed by the Hubble Space Telescope in programs during the interval 2005-2009. Our results indicate that both the northern and southern UV powers are dependent on SKR phase, varying diurnally by factors of similar to 3. We also show that the UV variation originates principally from the morning half of the oval, consistent with previous observations of the SKR sources. Citation: Nichols, J. D., B. Cecconi, J. T. Clarke, S. W. H. Cowley, J.-C. Gerard, A. Grocott, D. Grodent, L. Lamy, and P. Zarka (2010), Variation of Saturn's UV aurora with SKR phase, Geophys. Res. Lett., 37, L15102, doi: 10.1029/2010GL044057
Le lezioni-testimonianza. Analisi del contenuto
No full textNel contributo gli autori presentano i risultati dell'analisi del contenuto effettuata sul corpus testuale accumulato con i testi delle trascrizioni delle le lezioni-testimonianza e delle interviste. Il lavoro è stato condotto in una prima fase da coppie di ricercatori che hanno analizzato ciascuna un'area di contenuto (i tre quesiti alla base della ricerca): a) come apprendono i bambini; b) verso quali saperi sono orientati; c) con quali scelte didattiche. Laura Cerrocchi, Jovanka Rivi e Giusi Casasanta hanno scritto il par. 1 Come apprendono i bambini, pp.101-126, Antonio Gariboldi ha scritto il par. 2 I saperi verso cui gli insegnanti orientano i bambini, pp.126-143, Luciano Cecconi, dopo essersi confrontato con Paola Cagliari in merito all’analisi delle testimonianze sul tema “Le scelte didattiche degli operatori”, ha scritto il par. 3 "Le scelte didattiche degli operatori: contenuti e motivazioni", pp.144-184. Per quanto riguarda il par.1, nello specifico Laura Cerrocchi ha scritto il par. 1.3 per le voci: e, f, g, h, i, l, m e il par. 1.4. Jovanka Rivi ha scritto i parr.1 e 1.1 e il par. 1.2 per le voci a, b, c, d, e, f, g, h e i. Giusi Casasanta ha scritto il par. 1.2 per le voci l, m, n, o, p, q, r e il par. 1.3 per le voci a, b, c e d
Pancreatic endocrine tumours, a proteomic investigation of the trichostatin-A antitumoral effect
No full textThe effects of the histone-deacetylases inhibitor trichostatin A (TSA) on the growth of three different human pancreatic endocrine carcinoma cell lines (CM, BON and QGP-1), has been assessed via dosage-dependent growth inhibition curves. All cell lines showed strong inhibition of cell growth after TSA with similar IC50 values: 80.5 nM (CM), 61.6 nM (BON) and 86 nM (QGP-1). TSA treatment determined both cell cycle arrest in G2/M phase and apoptotis. 2D-PAGE analysis revealed 30, 39 and 29 different proteins differentially expressed after TSA treatment in the CM, BON and QGP-1 cell lines, respectively. The most important groups of modulated proteins, identified by nano RP-HPLC-ESI-MS/MS, belong to cell proliferation, cell cycle and apoptosis class (such as peroxiredoxins 1 and 2, the Diablo protein, HSP27). Other proteins belong to processes such as regulation of gene expression (nucleophosmin, oncoprotein-Dek), signal transduction (calcium-calmodulin), chromatin and cytoscheleton organization (calgizzarin, dynein, lamin), RNA splicing (nucleolin, HNRPC), and protein folding (HSP70). The present data are in agreement with previous proteomic analyses performed on pancreatic ductal carcinoma cell lines [Cecconi, D., et al., Electrophoresis 2003; Cecconi, D., et al., J Proteome Res. 2005] and place histone-deacetylases inhibitors among the potentially most powerful drugs for treatment of pancreatic tumours
Numerical approaches for quantitative analysis of two-dimensional maps: a review of commercial software and home-made systems.
No full textThe present review attempts to cover a number of methods that have appeared in the last few years for performing quantitative proteome analysis. However, due to the large number of methods described for both electrophoretic and chromatographic approaches, we have limited this review to conventional two-dimensional (2-D) map analysis which couples orthogonally a charge-based step (isoelectric focusing) to a size-based separation step (sodium dodecyl sulfate-electrophoresis). The first and oldest method applied to 2-D map data reduction is based on statistical analysis performed on sets of gels via powerful software packages, such as Melanie, PDQuest, Z3 and Z4000, Phoretix and Progenesis. This method calls for separately running a number of replicas for control and treated samples. The two sets of data are then merged and compared via a number of software packages which we describe. in addition to commercially-available systems, a number of home made approaches for 2-D map comparison have been recently described and are also reviewed. They are based on fuzzyfication of the digitized 2-D gel image coupled to linear discriminant analysis, three-way principal component analysis or a combination of principal component analysis and soft-independent modeling of class analogy. These statistical tools appear to perform well in differential proteomic studies
Gli strumenti dell'intervento didattico
No full textIl testo presenta una prima parte di analisi teorica sul ruolo dei mezzi didattici nei processi di insegnamento-apprendimento. L'analisi viene condotta sia dal punto di vista dei processi comunicativi sia da quello pedagogico. Nella seconda parte vengono presi in esame alcuni media educativi, con particolare riferimento alle tecnologie dell'informazione e della comunicazione. Un particolare approfondimento viene proposto per quanto riguarda i mezzi disponibili per la didattica museale (cd-rom e siti web)
The pro-autophagic protein AMBRA1 coordinates cell cycle progression by regulating CCND (cyclin D) stability
No full textThe scaffold protein AMBRA1 regulates the early steps of autophagosome
formation and cell growth, and its deficiency is associated with
neurodevelopmental defects and cancer. In a recent study, we show that
AMBRA1 is a key factor in the upstream branch of the MYCN-MYC and
CDK4-CDK6-dependent regulation of G1/S phase transition. Indeed, in the
developing neuroepithelium, in neural stem cells, and in cancer cells,
we demonstrate that AMBRA1 regulates the expression of D-type cyclins by
controlling both their proteasomal degradation and their
MYCN-MYC-mediated transcription. Also, we show that this regulation axis
maintains genome integrity during DNA replication, and we identify a
possible line of treatment for tumors downregulating AMBRA1 and/or
overexpressing CCND1 (cyclin D1), by demonstrating that AMBRA1-depleted
cells carry an AMBRA1-loss-specific lethal sensitivity to CHEK1
inhibition. Interestingly, we show that this aspect is specific for
AMBRA1 loss, because ATG7 knockdown does not display the same response
to CHEK1 inhibitors. Hence, our findings underscore that the
AMBRA1-CCND1 pathway represents a novel crucial mechanism of cell cycle
regulation, deeply interconnected with genomic stability in development
and cancer
Unique features in the mitochondrial D-loop region of the European seabass Dicentrarchus labrax
No full textWe have cloned and sequenced the displacement-loop (D-loop) region of the mitochondrial DNA (mtDNA) from the European seabass Dicentrarchus labrax (Dl). This sequencing revealed the presence of four tandemly repeated elements (R1, R2, R3 and R4); the individual variation in mtDNA total length is entirely accounted for by their variable number. The individuals examined also possessed an imperfect copy of one of the tandem repeats (psi R2). At least one termination-associated sequence (TAS) is present in each of the repeats and in two copies 5' upstream from the tandem array as well. The alignment of the Dl D-loop region with D-loop sequences from four other Teleosts and one Chondrosteus showed the Dl sequence to be larger than that of other fish. The extraordinary length of the Dl D-loop sequence is also due to the 5' and 3' regions that are flanking the tandem array, the largest ones to date analyzed in fish. In this study, we also report the unique organization and localization of putative TAS and conserved-sequence block (CSB) elements, and the presence of a conserved 218-bp sequence in the Dl D-loop region
Kindergarten Environments in Reggio Emilia, Bologna, Modena, and Parma, Italy. In Search of Quality
No full textGlobally, early childhood educators have been influenced by the municipally operated Reggio Emilia preschools of northern Italy. There are, however, three types of preschool settings in Italy—state, municipal, and private. The last year of the preschool experience is equivalent to the kindergarten year in the United States, and the preschool programs are also referred to as kindergartens. Italy is a progressive country that has embraced quality early childhood programs beginning with the post-World War II era. Italy offers government-supported preschool education with over 94% attendance; the program is full-day, five days a week and operates from early September through the end of June. The authors of this chapter conducted a pilot study of the ACEI Global Guidelines Assessment instrument in the northern Emilia Romagna region of Italy, including teachers from all three types of kindergarten classrooms. In this chapter the authors discuss kindergarten environments for young children 3 to 6 years of age. In addition, the results of a study of classroom environments using the global guidelines in these three kindergarten types are presented. Kindergarten classrooms in the northern sector of Italy—Bologna,Modena, Reggio Emilia, and Parma—are described in this chapter
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