91 research outputs found
Observaciones sobre Anacreonte, PMG 395
Full text linkThe goal of this paper is to conduct a rhythmical-melodical
analysis of Anacreon’s PMG 395. After
a presentation of the text, including a study of the main
discussions about it in scholarship, the author analyzes
it by studying the distribution of enjambments and accents
in the lines. Finally, he suggests that the conclusions
obtained have a scope that is not limited to PMG
395 in particular, but that they improve our understanding
of the poetic technique of Anacreon in general.El propósito del presente artículo es realizar un análisis rítmico-melódico del poema PMG 395 de Anacreonte. Después de una presentación sobre el texto, incluyendo un estudio de las principales discusiones sobre él en la bibliografía, el autor los analiza estudiando la distribución de encabalgamientos y de acentos en las líneas. Se sugiere, finalmente, que las conclusiones obtenidas tienen un alcance que no se limita a PMG 395 en particular, sino que mejoran nuestra compresió
Observations about Anacreon, PMG 395
Full text linkEl propósito del presente artículo es realizar un análisis rítmico-melódico del poema PMG 395 de Anacreonte. Después de una presentación sobre el texto, incluyendo un estudio de las principales discusiones sobre él en la bibliografía, el autor los analiza estudiando la distribución de encabalgamientos y de acentos en las líneas. Se sugiere, finalmente, que las conclusiones obtenidas tienen un alcance que no se limita a PMG 395 en particular, sino que mejoran nuestra compresión de la técnica poética de Anacreonte en sentido amplio.The goal of this paper is to conduct a rhythmical-melodical analysis of Anacreon’s PMG 395. After a presentation of the text, including a study of the main discussions about it in scholarship, the author analyzes it by studying the distribution of enjambments and accents in the lines. Finally, he suggests that the conclusions obtained have a scope that is not limited to PMG 395 in particular, but that they improve our understanding of the poetic technique of Anacreon in general.Fil: Abritta, Alejandro. Universidad de Buenos Aires. Facultad de Filosofía y Letras. Instituto de Filología Clásica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin
Characterization of a temperature-responsive two component regulatory system from the Antarctic archaeon, Methanococcoides burtonii
Full text linkCold environments dominate the Earth's biosphere and the resident microorganisms play critical roles in fulfilling global biogeochemical cycles. However, only few studies have examined the molecular basis of thermosensing; an ability that microorganisms must possess in order to respond to environmental temperature and regulate cellular processes. Two component regulatory systems have been inferred to function in thermal regulation of gene expression, but biochemical studies assessing these systems in Bacteria are rare, and none have been performed in Archaea or psychrophiles. Here we examined the LtrK/LtrR two component regulatory system from the Antarctic archaeon, Methanococcoides burtonii, assessing kinase and phosphatase activities of wild-type and mutant proteins. LtrK was thermally unstable and had optimal phosphorylation activity at 10 °C (the lowest optimum activity for any psychrophilic enzyme), high activity at 0 °C and was rapidly thermally inactivated at 30 °C. These biochemical properties match well with normal environmental temperatures of M. burtonii (0-4 °C) and the temperature this psychrophile is capable of growing at in the laboratory (-2 to 28 °C). Our findings are consistent with a role for LtrK in performing phosphotransfer reactions with LtrR that could lead to temperature-dependent gene regulation
2021 Home Grounds and Animals PMG - Author Contact List
No full textThis is a chapter of the 2021 Home Grounds and Animals PMG. This 2021 Virginia Pest Management Guide provides the latest recommendations for controlling diseases, insects, and weeds for home grounds and animals. This publication contains information about prevention and nonchemical control as alternatives to chemical control or as part of an integrated pest management approach. The chemical controls in this guide are based on the latest pesticide label information at the time of writing. Because pesticide labels change, read the label directions carefully before buying and using any pesticide. Regardless of the information provided here, always follow the latest product label instructions when using any pesticide. Commercial products are named in this publication for informational purposes only. Virginia Cooperative Extension does not endorse these products and does not intend discrimination against other products that also may be suitable.Peer reviewe
Patterning of the MinD cell division protein in cells of arbitrary shape can be predicted using a heuristic dispersion relation
Full text link© 2016, Paul M. G. Curmi, et al. Many important cellular processes require the accurate positioning of subcellular structures. Underpinning many of these are protein systems that spontaneously generate spatiotemporal patterns. In some cases, these systems can be described by non-linear reaction-diffusion equations, however, a full description of such equations is rarely available. A well-studied patterning system is the Min protein system that underpins the positioning of the FtsZ contractile ring during cell division in Escherichia coli. Using a coordinate-free linear stability analysis, the reaction terms can be separated from the geometry of a cell. The reaction terms produce a dispersion relation that can be used to predict patterning on any cell shape and size. Applying linear stability analysis to an accurate mathematical model of the Min system shows that while it correctly predicts the onset of patterning, the dispersion relation fails to predict oscillations and quantitative mode transitions. However, we show that data from full solutions of the Min model can be used to generate a heuristic dispersion relation. We show that this heuristic dispersion relation can be used to approximate the Min protein patterning obtained by full simulations of the non-linear reaction-diffusion equations. Moreover, it also predicts Min patterning obtained from experiments where the shapes of E. coli cells have been deformed into rectangles or arbitrary shapes. Using this procedure, it should be possible to generate heuristic dispersion relations from protein patterning data or simulations for any patterning process and subsequently use these to predict patterning for arbitrary cell shapes
Design and Experimental Studies on Superconducting Maglev Systems With Multisurface HTS-PMG Arrangements
No full textIn this article, we have designed and constructed a new multisurface (MS) high temperature superconductor (HTS) Maglev measurement system to investigate the enhancement of magnetic force properties of Maglev systems via MS HTS configurations above conventional permanent magnetic guideway (PMG). We have investigated both the static force and stiffness behavior and dynamic response characteristics of these MS HTS-PMG arrangements in different field cooling heights (FCHs). Optimum cooling height is determined as FCH 20-30 for both six- and four-HTS configurations. The maximum levitation force values of HTS-PMG arrangement with six-HTS were obtained bigger than that of four-HTS in the unit cryostat volume of MS arrangement, indicating that the HTSs at the bottom side of the cryostat make contribution to the loading capacity of Maglev systems. In the present article, it is observed that the magnetic flux density of bottom surface in addition to upper surface of the PMG can make a contribution to loading performance, vertical and lateral stability of Maglev systems. It is thought that the designed measurement facility and results of this study will be beneficial to increase the magnetic flux density in the unit volume via MS HTS-PMG arrangements for future design and construction of the HTS Maglev systems.Scientific and Technological Research Council of Turkey (TUBITAK-Turkey) [118F426]Manuscript received March 7, 2021; revised April 15, 2021; accepted May 26, 2021. Date of publication May 31, 2021; date of current version June 17, 2021. This work was supported by the Scientific and Technological Research Council of Turkey (TUBITAK-Turkey) under Project 118F426. This article was recommended by Associate Editor M. Zhang. (Corresponding author: Murat Abdioglu.
Single TRAM domain RNA-binding proteins in Archaea: functional insight from Ctr3 from the Antarctic methanogen Methanococcoides burtonii
Full text linkTRAM domain proteins present in Archaea and Bacteria have a β-barrel shape with anti-parallel β-sheets that form a nucleic acid binding surface; a structure also present in cold shock proteins (Csps). Aside from protein structures, experimental data defining the function of TRAM domains is lacking. Here, we explore the possible functional properties of a single TRAM domain protein, Ctr3 (cold-responsive TRAM domain protein 3) from the Antarctic archaeon Methanococcoides burtonii that has increased abundance during low temperature growth. Ribonucleic acid (RNA) bound by Ctr3 in vitro was determined using RNA-seq. Ctr3-bound M. burtonii RNA with a preference for transfer (t)RNA and 5S ribosomal RNA, and a potential binding motif was identified. In tRNA, the motif represented the C loop; a region that is conserved in tRNA from all domains of life and appears to be solvent exposed, potentially providing access for Ctr3 to bind. Ctr3 and Csps are structurally similar and are both inferred to function in low temperature translation. The broad representation of single TRAM domain proteins within Archaea compared with their apparent absence in Bacteria, and scarcity of Csps in Archaea but prevalence in Bacteria, suggests they represent distinct evolutionary lineages of functionally equivalent RNA-binding proteins
Molecular Interactions of the Min Protein System Reproduce Spatiotemporal Patterning in Growing and Dividing Escherichia coli Cells
Full text link© 2015 Walsh et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Oscillations of the Min protein system are involved in the correct midcell placement of the divisome during Escherichia coli cell division. Based on molecular interactions of the Min system, we formulated a mathematical model that reproduces Min patterning during cell growth and division. Specifically, the increase in the residence time of MinD attached to the membrane as its own concentration increases, is accounted for by dimerisation of membrane- bound MinD and its interaction with MinE. Simulation of this system generates unparalleled correlation between the waveshape of experimental and theoretical MinD distributions, suggesting that the dominant interactions of the physical system have been successfully incorporated into the model. For cells where MinD is fully-labelled with GFP, the model reproduces the stationary localization of MinD-GFP for short cells, followed by oscillations from pole to pole in larger cells, and the transition to the symmetric distribution during cell filamentation. Cells containing a secondary, GFP-labelled MinD display a contrasting pattern. The model is able to account for these differences, including temporary midcell localization just prior to division, by increasing the rate constant controlling MinD ATPase and heterotetramer dissociation. For both experimental conditions, the model can explain how cell division results in an equal distribution of MinD and MinE in the two daughter cells, and accounts for the temperature dependence of the period of Min oscillations. Thus, we show that while other interactions may be present, they are not needed to reproduce the main characteristics of the Min system in vivo
Non-linear Min protein interactions generate harmonics that signal mid-cell division in Escherichia coli
Full text link© 2017 Walsh et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. The Min protein system creates a dynamic spatial pattern in Escherichia coli cells where the proteins MinD and MinE oscillate from pole to pole. MinD positions MinC, an inhibitor of FtsZ ring formation, contributing to the mid-cell localization of cell division. In this paper, Fourier analysis is used to decompose experimental and model MinD spatial distributions into time-dependent harmonic components. In both experiment and model, the second harmonic component is responsible for producing a mid-cell minimum in MinD concentration. The features of this harmonic are robust in both experiment and model. Fourier analysis reveals a close correspondence between the time-dependent behaviour of the harmonic components in the experimental data and model. Given this, each molecular species in the model was analysed individually. This analysis revealed that membrane-bound MinD dimer shows the mid-cell minimum with the highest contrast when averaged over time, carrying the strongest signal for positioning the cell division ring. This concurs with previous data showing that the MinD dimer binds to MinC inhibiting FtsZ ring formation. These results show that non-linear interactions of Min proteins are essential for producing the mid-cell positioning signal via the generation of second-order harmonic components in the time-dependent spatial protein distribution
Point mutations in the transmembrane region of the clic1 ion channel selectively modify its biophysical properties.
Full text linkChloride intracellular Channel 1 (CLIC1) is a metamorphic protein that changes from a soluble cytoplasmic protein into a transmembrane protein. Once inserted into membranes, CLIC1 multimerises and is able to form chloride selective ion channels. Whilst CLIC1 behaves as an ion channel both in cells and in artificial lipid bilayers, its structure in the soluble form has led to some uncertainty as to whether it really is an ion channel protein. CLIC1 has a single putative transmembrane region that contains only two charged residues: arginine 29 (Arg29) and lysine 37 (Lys37). As charged residues are likely to have a key role in ion channel function, we hypothesized that mutating them to neutral alanine to generate K37A and R29A CLIC1 would alter the electrophysiological characteristics of CLIC1. By using three different electrophysiological approaches: i) single channel Tip-Dip in artificial bilayers using soluble recombinant CLIC1, ii) cell-attached and iii) whole-cell patch clamp recordings in transiently transfected HEK cells, we determined that the K37A mutation altered the single-channel conductance while the R29A mutation affected the single-channel open probability in response to variation in membrane potential. Our results show that mutation of the two charged amino acids (K37 and R29) in the putative transmembrane region of CLIC1 alters the biophysical properties of the ion channel in both artificial bilayers and cells. Hence these charged residues are directly involved in regulating its ion channel activity. This strongly suggests that, despite its unusual structure, CLIC1 itself is able to form a chloride ion channel
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