4,705 research outputs found
Studi sul caporalato
No full textIl volume vuole essere un approccio interdisciplinare ad un settore, quello della intermediazione illecita e dello sfruttamento del lavoro, dove - con la legge n. 199/2016 - si coglie la nitida consapevolezza delle ricadute della giustizia sull'economia e della necessità di operare in modo che la responsabilità penale o amministrativa rimanga circoscritta alle persone fisiche o giuridiche cui l’illecito è attribuibile. Il diffuso lavoro sommerso, la persistente crisi economica, l'ingresso della criminalità organizzata nella gestione di gruppi vulnerabili hanno portato a conoscere la figura del caporale anche in settori diversi da quello agricolo, trasformandola in componente dell'organizzazione criminale
Yeast metabolism in fresh and frozen dough : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Food Technology at Massey University, Palmerston North, New Zealand
No full textAuthor also known as SM LovedayFresh bakery products have a very short shelf life, which limits the extent to which manufacturing can be centralised. Frozen doughs are relatively stable and can be manufactured in large volumes, distributed and baked on-demand at the point of sale or consumption. With appropriate formulation and processing a shelf life of several months can be achieved.Shelf life is limited by a decline in proofing rate after thawing, which is attributed to a) the dough losing its ability to retain gas and b) insufficient gas production, i.e. yeast activity. The loss of shelf life is accelerated by delays between mixing and freezing, which allow yeast cells the chance to ferment carbohydrates.This work examined the reasons for insufficient gas production after thawing frozen dough and the effect of pre-freezing fermentation on shelf life. Literature data on yeast metabolite dynamics in fermenting dough were incomplete. In particular there were few data on the accumulation of ethanol, a major fermentation end product which can be injurious to yeast.Doughs were prepared in a domestic breadmaker using compressed yeast from a local manufacturer and analysed for glucose, fructose, sucrose, maltose and ethanol. Gas production after thawing declined within 48 hours of frozen storage. This was accelerated by 30 or 90 minutes of fermentation at 30;C prior to freezing.Sucrose was rapidly hydrolysed and yeast consumed glucose in preference to fructose. Maltose was not consumed while other sugars remained. Ethanol, accumulated from consumption of glucose and fructose, was produced in approximately equal amounts to CO2, indicating that yeast cells metabolised reductively.Glucose uptake in fermenting dough followed simple hyperbolic kinetics and fructose uptake was competitively inhibited by glucose. Mathematical modelling indicated that diffusion of sugars and ethanol in dough occurred quickly enough to eliminate solute gradients brought about by yeast metabolism
Supplementary data of the review paper "Specialized metabolite modifications in Brassicaceae seeds and plants: diversity, functions and related enzymes""
No full textSupplementary tables of the review paper "Specialized metabolite modifications in Brassicaceae seeds and plants: diversity, functions and related enzymes".
Supplementary Table 1. Characterised enzymes.
Enzyme category: AT = acyltransferase, GT = Glycosyltransferase, HL = hydroxylase, MT = methyltransferase. Subcategory: SCPL = Serine carboxypeptidase-like, GT1 = Glycosyltransferase family 1, 2-ODD = 2-oxoglutarate-dependent dioxygenase, CYP450 = cytochrome P450, O-MT = O-methyltransferase.
Supplementary Table 2. CYP450 enzyme families specificities of substrate and corresponding number of genes in Brassicaceae species.
This table regroups and summarizes informations that have been found in "The Plant Cytochrome P450 Database" database (https://erda.dk/public/vgrid/PlantP450/table.html (Hansen et al. (2021), consulted in January 2023) and review articles from Xu et al. (2015) and Yu et al. (2017). The attribution of substrate specificities of CYP450 enzyme families was made according to the known substrate specificities of CYP450 enzyme members of each family.
Hansen et al. (2021) - Molecular Plant, 2021, 14, 1244–1265 DOI: 10.1016/j.molp.2021.06.028
Xu et al. (2015) - Journal of Integrative Agriculture, 14(9): 1673–1686 DOI: 10.1016/S2095-3119(14)60980-1
Yu et al. (2017) - BMC Genomics, 18:733 DOI: 10.1186/s12864-017-4094-7
Supplementary Table 3. List of putative and characterised modification enzymes used for heatmap and phylogenetic tree analyses.
Category: AT = acyltransferase, GT = Glycosyltransferase, HL = hydroxylase, MT = methyltransferase. Subcategory: GT1 = Glycosyltransferase family 1, 2-ODD = 2-oxoglutarate-dependent dioxygenase, CYP450 = cytochrome P450. Gene list accessions for each SM decoration enzyme category was established by combining key-word (e.g. “Hydroxylase”, “CYP450”, “2-oxoglutarate-dependent dioxygenase”, “SAM-dependent methyltransferase”, “O methyltransferase”, “Glycosyltransferase”, “Acyltransferase”, “SCPL”, “BAHD acyltransferase”)results research from The Arabidopsis Information Resource (TAIR - www.arabidopsis.org/), National Center for Biotechnology Information (NCBI, www.ncbi.nlm.nih.gov/) and the Carbohydrate-Active enZYmes (CAZy database - www.cazy.org; Drula et al., 2022) databases, together with lists found in Bontpart et al. 2015 (for acyltransferases) and Kawai et al. 2014 (for 2-oxoglutarate-dependent dioxygenases)
Bontpart et al. (2015) - New Phytologist, 208(3):695-707. doi: 10.1111/nph.13498.
Drula et al. (2022) - Nucleic Acids Research, 50(D1): D571–D577 doi.org/10.1093/nar/gkab1045
Kawai et al. (2014) - The Plant Journal, Apr;78(2):328-43. doi: 10.1111/tpj.12479.
Supplementary Figure 1. Phylogenetic trees of A. thaliana enzymes putatively involved SM modifications.
a) GT1 glycosyltransferases. b) O-methyltransferases. c) Acyltransferases. d) Hydroxylases. Different protein names are separated by slash. “*” means that the locus is expressed in seeds.
Enzyme amino acid sequences were retrieved from The Arabidopsis Information Resource (TAIR; https://www.arabidopsis.org/; genome release Araport11). Multiple alignments of Arabidopsis hydroxylases, methyltransferases, glycosyltransferases and acyltransferases (See Supplementary Table 2 for accessions list) sequences were performed using MUSCLE with default options (Edgar, 2004).
The phylogenetic tree was inferred using the Neighbor-Joining (NJ). Bootstrap values were calculated for the highest iterations as possible (1000 for GT1 glycosyltransferases, 986 for methyltransferases, 284 for BAHD acyltransferases, 131 for SCPL acyltransferases, 760 for CYP450 hydroxylases and 993 for the rest of hydroxylases). The optimal trees are shown. Trees are drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic trees. The evolutionary distances were computed using the Poisson correction method and are in the units of the number of amino acid substitutions per site. All ambiguous positions were removed for each sequence pair (pairwise deletion option). Evolutionary analyses were conducted in MEGA11 (Tamura et al., 2021)
Converting SrI <sub>2</sub> :Eu <sup>2+</sup> into a near infrared scintillator by Sm <sup>2+</sup> co-doping
No full text The luminescence and scintillation properties of SrI 2 single crystals doped with 5% Eu 2+ and 0.05%, 0.2% and 0.5% Sm 2+ are evaluated. X-ray excited and photoluminescence measurements show energy transfer from excited Eu 2+ ions to Sm 2+ ions. At a concentration of 0.5% Sm 2+ , the luminescence consists almost entirely of 740 nm emission from Sm 2+ 5d-4f transitions. Co-doping SrI 2 :5% Eu 2+ with Sm 2+ provides a novel method to bypass the self-absorption problem encountered in large SrI 2 :Eu 2+ crystals and, at the same time, provides a unique near-infrared emitting scintillator with a light yield of approximately 40,000 photons/MeV. Accepted Author ManuscriptRST/Fundamental Aspects of Materials and EnergyRST/Luminescence Material
'Laws 'Needefull in Later to be Abrogated': Intersex and the Sources of Christian Theology
No full textThis is the author accepted manuscript. The final version is available from Palgrave Macmillan via the DOI in this record
Introduction: Troubling Bodies?
No full textThis is the author accepted manuscript. The final version is available from Palgrave Macmillan via the DOI in this record
Intrafullerene electron transfers in Sm-containing metallofullerenes: Sm@C-2n (74 <= 2n <= 84)
No full textThe electronic properties of Sm-containing metallofullerenes, Sm@C-74, Sm@C-76 (I, II), Sm@C-78, Sm@C-80, Sm@C-82 (I, II, III) and Sm@C-84 (I, II, III), are characterized by UV-Vis-NIR absorption spectroscopy and electron energy-loss spectroscopy (EELS). the UV-Vis-NIR absorption spectra of Sm@C-74, Sm@C-80, Sm@C-82 (I, II, III) and Sm@C-84 (I, II) are quite similar to those of the corresponding Ca, Sr, Ba, Eu, Tm, Yb-based metallofullerenes. In contrast, the absorption spectra of Sm@C-76 (I, II), Sm@C-78 and Sm@C-84(III) show a novel feature: the onset for Sm@C-78 is observed similar to 2600 nm, which corresponds to a small band gap (similar to0.5 eV). Furthermore, the oxidation states of Sm atom in the various fullerene cages are investigated by EELS, which reveals that the Sm atom takes +2 oxidation state in the fullerene cages. A probable rationale for the tendency to have the Sm2+ state is presented based on a simple thermochemical cycle model. (C) 2001 by Elsevier Science Inc.http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000168906500014&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701Biochemical Research MethodsBiochemistry & Molecular BiologyComputer Science, Interdisciplinary ApplicationsCrystallographyMathematical & Computational BiologySCI(E)EI30ARTICLE2244-2511
beta-decay spectroscopy of neutron-rich Sm-160,Sm-161,Sm-162 isotopes
No full textNeutron-rich Sm-160,Sm-161,Sm-162 isotopes have been populated at the RIBF, RIKEN via beta decay for the first time. beta-coincident gamma rays were observed in all three isotopes including gamma rays from the isomeric decay of Sm-160 and Sm-162. The isomers in Sm-160 and Sm-162 have previously been observed but have been populated via beta decay for the first time. The isomeric state in Sm-162 is assigned a 4(-) nu 7/2(+)[633]circle times nu 1/2(-)[521] configuration based on the decay pattern. The level schemes of Sm-160 and Sm-162 are presented. The ground states in the parent nuclei Pm-160 and Pm-162 are both assigned a 6(-) nu 7/2(+)[633]circle times pi 5/2(-)[532] configuration based on the population of states in the daughter nuclei. Blocked BCS calculations were performed to further investigate the spin-parities of the ground states in Pm-160, Pm-161, and Pm-162, and the isomeric state in Sm-162.CPCI-S(ISTP)[email protected]
IMPATTO SOCIO-ECONOMICO DELLA SCLEROSI MULTIPLA (SM) NEL NORD SARDEGNA
No full textLa SM è una patologia demielinizzante progressiva ad ampia diffusione ed a esordio precoce. In Italia risultano affette circa
58.000 persone ed in Sardegna una ogni 700 abitanti. Compare con maggiore frequenza nella donne (2:1 rispetto agli
uomini).Gli approcci terapeutici utilizzati sono diversi e non risolutivi: variano dai corticosteroidi (prednisone, desametasone) ed
altri immunosoppressori (azatioprina, metotressato), agli immunostimolanti (beta interferoni, glatiramer acetato) fino ai nuovi
immunosoppressori selettivi (natalizumab, fingolimod). Recentemente l'AIFA ha approvato l’uso, come antispastico, di un
preparato a base di cannabinoidi. Controverso è l’uso di dimetil fumarato, biocida antimuffa, approvato dalla FDA per le forme
recidivanti.Considerata l’ampia diffusione e l’impatto sociale ed economico della SM, sono stati esaminati i casi clinici seguiti dal
Centro di riferimento per la SM nel nord Sardegna (Clinica Neurologica AOU-Sassari) al fine di valutare l’evoluzione della
patologia in corso di trattamento, il costo dei trattamenti e l’incidenza sulle risorse del SSR negli anni 2012-2013
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