86,526 research outputs found
Re-semantization of the “Via Francigena” in Apulia
No full textGli antichi percorsi medievali possono costituire l’emblema di un turismo sostenibile; pertanto questo testo propone la risemantizzazione dell’antico percorso della via Francigena in Puglia. Tale itinerario viene legato alla rivalutazione dei beni culturali legati alla presenza degli ordini religioso-cavallereschi nella nostra regione
La Risemantizzazione della Via Francigena e Valorizzazione dei Beni Culturali afferenti agli Ordini Religiosi-Cavallereschi
No full text
CHE COS'E' UNA CITTA'?
No full textReinteroretazione della natura del fatto urbano e dellesue origini e funzioni
Quantitative in situ hybridization for the evaluation of gene expression in asynchronous and synchronized cell cultures and in tissue sections
No full textWe describe an image analysis (IA) system that has been applied for the quantitative evaluation of mRNAs evidenced by in situ hybridization (ISH) with radiolabelled probes in cultured cells and in tissue sections. The ISH-IA method was used for the evaluation of cultured cell morphological parameters such as cell and nucleous area (CA and NA, respectively) in parallel with the levels of mRNAs detected as hybridization grains areas (GA). The evaluation of these parameters, together with the analysis of the levels of mRNAs (c-jun, cyclin A) specific for given cell cycle phases (i.e. G1 and S/G2), allowed the identification, in asynchronous cultures of human skin fibroblasts, of cells in G1 and S/G2 phases. The mRNA levels measured by ISH-AI were comparable with those detected by RT-PCR. This method was also applied for the analysis of fibronectin (FN) gene expression in control skin fibroblasts in relationship with the different phases of the cell cycle and in comparison with a tumor cell line (Sk-Hep1), heterogeneous either for morphometric parameters or for the levels of this transcript. Finally, the ISH-AI was applied for the semiquantitative evaluation of the expression, localization and alternative splicing pattern of FN mRNA in normal liver and in hepatocellular carcinoma (HCC) tissue sections
The Sassi and the Park of the Rupestrian Churches of Matera: rehabilitation, cultural tourism and local development policies
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