11,042 research outputs found
DNA modulates solvent isotope effects in a nanopore
No full textHere we investigate the modulation of solvent isotope effects by the entry of DNA molecules into individual α-haemolysin nanopores. Solvent isotope effects in D2O versus H2O were enhanced (kH/kD ≈ 1.6) compared to the bulk (kH/kD ≈ 1.2), except when the pore was most blocked (kH/kD ≤ 1.1).Ion current recordings, raw data as recorded in PClamp 10.2 software. NOTE: Some files are in Axon(TM) Binary Format (ABF), a format proprietary to Molecular Devices
Can dispersion forces govern aromatic stacking in an organic solvent?
No full textExperimental support for the dominance of van der Waals dispersion forces in aromatic stacking interactions occurring in organic solution is surprisingly limited. Here, we have examined the size-dependence of aromatic stacking in an organic solvent. The interaction energy was found to vary by ~7.5 kJ mol–1 on going from a phenyl-phenyl to an anthracene-pyrene stack. Strikingly, the experimental data were highly correlated with dispersion energies determined using symmetry-adapted perturbation theory (SAPT), while the induction, exchange, electrostatic and solvation energy components correlated poorly. Both the experimental data and the SAPT-dispersion energies gave high-quality correlations with the change in solvent accessible area upon complexation. Thus, the size-dependence of aromatic stacking interactions is consistent with the dominance of van der Waals dispersion forces even in the presence of a competing polarizable solvent..xyx coordinates of minimised complex geometries, NMR characterisation and titration data
Molecular balances for measuring non-covalent interactions in solution
No full textNon-covalent interactions in solution are subject to modulation by surrounding solvent
molecules. This thesis presents two experimental molecular balances that have been
used to quantify solvent effects on non-covalent interactions, including electrostatic
and dispersion interactions.
The first chapter introduces literature where non-covalent interactions have been
studied in a range of solvents, particularly those where the effects of aqueous or
fluorous solvents have been investigated. These solvents are of particular interest as
they both invoke solvophobic effects on organic molecules, but have differing
chemical and physical properties.
The second chapter describes the adaptation of the Wilcox molecular torsion balance
to study interactions between organic and fluorinated carbon chains in a range of
solvents. Solvent cohesion was found to be the principle force driving both the alkyl
and fluorous chains together in aqueous solvents, where no contribution to the
interaction energy arising from dispersion forces could be detected. In fluorous and
polar organic solvents evidence was found for weak favourable dispersion interactions
between the alkyl chains. In contrast dispersion forces between the chains were found
to be disrupted by competitive van der Waals interactions with surrounding solvent
molecules in apolar organic solvents. Association of the fluorous chains was found to
be solely driven by solvent cohesion.
The final chapter describes the design and synthesis of a novel synthetic molecular-balance
framework and describes its application to simultaneously measure solvent
and substituent effects on the position of conformational equilibria. Despite the
simplicity of the model system, surprisingly complicated behaviour emerged from the
interplay of conformational, intramolecular and solvent effects. Nonetheless, a large
data set of experimental equilibrium constants was analysed using a simple solvent
model, which was able to account for both the intuitive and more unusual patterns
observed. A means of dissecting electrostatic and solvent effects to reveal pseudo gas-phase
behaviour has resulted from the analysis of experimental data obtained in many
solvents
Letter From William Bell Scott to Mr Chambers
No full textabstract: Concerning Scott's thanks, his writings about his own works, and a manuscript of "The Nightingale Unheard."Seller's Description: Reads "A.L.S. from Author to Mr. Chambers explaining how busy he is... The sonnet is printed in the book. Fredeman: 56.7 £87.50"Handwritten Note: Unknown handwriting at top right reads "June 1st 1877."Publication Details: "The Nightingale Unheard" published in "Poems" by William Bell Scott.Creation Date Details: Undated range is the author's lifespan.Provenance: Removed from:
Poems / by William Bell Scott. Ballads, studies from nature, sonnets, etc. / illustrated by seventeen etchings by the author and L. Alma Tadema. Publisher London : Longmans, Green, 1875. CALL #
HAYDEN SPECIAL COLL SPEC PRB-13
DNA modulates solvent isotope effects in a nanopore
No full textIon current recordings, raw data as recorded in PClamp 10.2 software. NOTE: Some files are in Axon(TM) Binary Format (ABF), a format proprietary to Molecular Devices.Here we investigate the modulation of solvent isotope effects by the entry of DNA molecules into individual α-haemolysin nanopores. Solvent isotope effects in D2O versus H2O were enhanced (kH/kD ≈ 1.6) compared to the bulk (kH/kD ≈ 1.2), except when the pore was most blocked (kH/kD ≤ 1.1).Watson, Matthew; Cockroft, Scott. (2015). DNA modulates solvent isotope effects in a nanopore, 2013-2015 [dataset]. University of Edinburgh, EaStCHEM School of Chemistry. http://dx.doi.org/10.7488/ds/277
Protein nanopores as a platform for transmembrane nanodevices
Full text linkNanopore sensing has seen vast development over the past four decades.
The technique originally looked to use electrophysiological methods to study
native protein channels. However, it is now possible to exploit these proteins
for sensing applications. Herein, we explore methods for covalent and
non-covalent modification of a biological nanopore to achieve new functionality.
Chapter 1 summarises the history of nanopore technology; from its inception
as a method for studying native channels, to its deployment in nanopore
sensing. To achieve effective sensing, native proteins have undergone a broad
range of chemical modification to achieve enhanced functionality. This chapter
explores the amalgamation of biological and solid-state nanopores.
Chapter 2 seeks to the monitor the binding and catalytic turnover of substrates
within a single cucurbituril molecule captured within a protein nanopore.
Previous work has shown that cucurbiturils and cyclodextrins can transiently
interact with an α-hemolysin channel. Capture of a single cucurbituril within a
protein nanopore was achieved, and the dwell time of the binding events was
optimised. Following this, it was demonstrated that observations of the
catalysed Diels-Alder could be made at the single-molecule level. However,
further optimisation of the resolution would be required to elucidate
mechanistic information.
Chapter 3 presents methods for in situ chemical functionalisation of a
biological nanopore. Here, the focus is upon the chemical modification of a
wild-type protein thereby to circumventing the need for mutagenesis. Three
target residues are discussed: lysine, methionine and tyrosine. Successful
modification was achieved at both the lysine and methionine sites of
α-hemolysin. While some provisional success was recorded with tyrosine, the
modifications were not reproducible.
Chapter 4 introduces preliminary work towards the development of
transmembrane molecular machines. This utilises the lysine modification
discussed in Chapter 2 to covalently attach established synthetic molecular
machines to the channel. Molecular switches, motors and pumps were all
explored. Some success was achieved attaching the molecular machines to a
protein channel. However, issues with pore stability limited the progress and
true machine-like behaviour was not observed
Scott Belsky in Conversation with DesignByThem - Part One.mp4
No full textWatch here as Scott Belsky (CEO/Founder Behance + Author Making Ideas Happen) joins Sydney design duo Sarah Gibson and Nicholas Karlovasitis from DesignByThem to discuss some of the challenges facing creative companies as they try to grow their business. In part one Nick and Sarah get some great advice from Scott about what makes an effective partnership and how to learn from the challenges that can arise. Scott also explains the Behance technique of ?Concepting Teams? with representatives from all areas of the business to brainstorm issues or new ideas
Cardozo AELJ Author Interview Series: Scott Semaya, Class of 2023
No full textThe Cardozo AELJ Author Interview Series seeks to give our readers further insight into the Articles and Notes published in the Cardozo Arts & Entertainment Law Journal. In this interview, Scott Semaya discusses his Note, Name, Image and Likeness: Giving College Athletes the Clearest Guidance to Best Profit off Their NIL, which was published in Volume 41, Issue 2.
This post was originally published on the Cardozo Arts & Entertainment Law Journal website on June 6, 2023. The original post can be accessed via the Archived Link button above
Cardozo AELJ Author Interview Series: Scott Semaya, Class of 2023
Full text linkThe Cardozo AELJ Author Interview Series seeks to give our readers further insight into the Articles and Notes published in the Cardozo Arts & Entertainment Law Journal. In this interview, Scott Semaya discusses his Note, Name, Image and Likeness: Giving College Athletes the Clearest Guidance to Best Profit off Their NIL, which was published in Volume 41, Issue 2.
This post was originally published on the Cardozo Arts & Entertainment Law Journal website on June 6, 2023. The original post can be accessed via the Archived Link button above
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Cardozo AELJ Author Interview Series: Scott Semaya, Class of 2023
No full textThe Cardozo AELJ Author Interview Series seeks to give our readers further insight into the Articles and Notes published in the Cardozo Arts & Entertainment Law Journal. In this interview, Scott Semaya discusses his Note, Name, Image and Likeness: Giving College Athletes the Clearest Guidance to Best Profit off Their NIL, which was published in Volume 41, Issue 2.
This post was originally published on the Cardozo Arts & Entertainment Law Journal website on June 6, 2023. The original post can be accessed via the Archived Link button above
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