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    Effects of pre-slaughter sheep handling and animal-related factors on creatine kinase levels and physico-chemical attributes of mutton

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    Effects of pre-slaughter sheep handling and animal-related factors on Creatine Kinase levels and physico-chemical attributes of mutton By Chulayo Amanda Yucca The objective of the study was to determine the effects of pre-slaughter sheep handling and animal-related factors on Creatine kinase (CK) levels and physico-chemical characteristics of mutton from South African sheep breeds. One hundred and seventy-three castrated male sheep from four breeds (15 Dormer, 46 South African Mutton Merino, 77 Dorper and 35 Blackhead Persian) were used. The animals were grouped according to age categories. The grouping was as follows: Group 1 (6 ≤ 8 months), Group 2 (9 ≤ 12 months) and Group 3 (13 ≤ 16 months). Blood samples for CK determination were collected at exanguination using disposable vacutainer tubes. Representative samples of the Muscularis longissimuss thoracis et. lumborum (LTL) muscle were taken for the determination of ultimate pH (pHu), colour (L*-lightness, b*- redness and a*- yellowness), thawing and cooking losses and meat tenderness. Significant (P<0.01) breed effects were observed on CK levels with Dormer having the highest CK values. Significant linear relationships were observed between pre-slaughter condition and CK levels. There were positive correlations between colour coordinates L* and b* (r =+0.22) and between a* and b* (r =+0.63). Cooking loss and Warner Braztler Shear Force (WBSF) values were positively correlated (r =+0.29). The Principal Component Analysis (PCA) showed a significant variance for CK and between physico-chemical characteristics of mutton. There were no relationships between CK levels and physico-chemical characteristics of mutton. Significant breed and age effects on pHu, L, a*, b*, thaw %, CL and WBSF of mutton were observed. iv It was concluded that, pre-slaughter conditions affect CK levels and physico-chemical characteristics of mutton. However, there were no relationships between CK levels and physico-chemical characteristics of mutton

    Experiences of students living with HIV and AIDS (SLWHA) regarding support group in a selected university campus, Eastern Cape Province

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    South Africa has one of the highest HIV and AIDS epidemic statistics in the world, especially people under 25 years of age (Department of Social Development, 2002:11). Some university students are implicated, thus Support groups in this context are crucial to help them cope with their unique circumstances. Purpose: of this study was to assess the effectiveness of support groups for SLWHA within a university campus in the Eastern Cape Province. Method: Interviews were used to explore the experiences and challenges of SLWHA. Results: The study shows that support groups are pivotal in the wellbeing of SLWHA in meeting diverse needs such as sense fo belonging, emotional, psychological, information and knowledge,socio-economic as well as health and physical needs. Support group members experience relatively similar living experiences, dynamic as their various lives are, they are able to share their emotions and problems arising from their HIV statuses, thereby lessening their burdens. Some negative experiences were observed, like issues of confidentiality, depression and stress. Conclusion: University support groups can be improved through structured programs that includes various stakeholders like health professionals (wellness centre), university management, and others including academics within the university. Also, expanding their borders via the use of social networks, and expanding their activities to include music, sports, vegetable gardening, group visitations, skills training, and other useful projects shall help to improve lives of victims and fight against the infection of other community members

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    The main objective of the current study was to determine the effect of distance travelled (DT), lairage duration (LDhr) and animal-related factors (animal class, breed, sex) on pre-slaughter stress indicators, carcass characteristics, nanostructure and technological properties of beef. The study was conducted in an Eastern Cape abattoir under the Buffalo City District Municipality. Six genotypes of cattle (Beefmaster, Bonsmara, Brahman, Holstein-Friesian, Non-descript and Nguni) brought for slaughter at the abattoir on different days were used in the study. Slaughter weight (SWT), warm and cold carcass weight (WCW and CCW), fatness and conformation of each animal were measured and recorded. Representative samples of blood plasma and meat quality from the Muscularis thoracis et lumborum (LTL) were collected at slaughter and 24 hours after slaughter, respectively. Heat shock proteins 70kDa (HSPA1A), glucose (GLU), cortisol (CORT), creatine kinase (CK) and lactate dehydrogenase (LDH) were determined from the blood plasma. The representative LTL muscle was used to determine ultimate pH (pHu), lightness (L*), redness (a*), yellowness (b*), meat temperature (Tm), Warner Bratzler Shear Force (WBSF) and histological structure of beef. There were significant effects of distance on pHu, Tm, a*, b*, TL%, CK and LDH. Pearson correlations were observed between distance travelled (DT), creatine kinase (CK), lactate dehydrogenase (LDH), ultimate pH (pHu), colour coordinates (L*, a* and b*) and Warner Bratzler Shear Force (WBSF). Principal component analysis (PCA) revealed that the 1st (pHu), 2nd (Tm) and 3rd (L*) principal components were the critical meat quality attributes. Positive relationships between carcass weights and beef quality characteristics for bovine cattle where the cumulative percentage of the first six principal component`s (PC`s) (SWT, WCW, CCW, DP, pHu and Tm contributed to 93.4 % of the total variance. Significant correlation coefficients between DT (r = 0.15), LDhr (r = 0.29), Ta (r = -0.52), Tm (r = 0.26), L* (r = 0.33), a* (r = 0.32) and WBSF (r = -0.49) were observed. Meat from Brahman (BR), Non-descript (ND) and Nguni (NG) cows had visible skeletal muscle fibres which were thin and long indicating improved tenderness of beef. Exposing animals to longer hours of transportation did not only reduce glucose levels but also increased the expression of HSPA1A and levels of cortisol. The CK and LDH were not related to beef quality. Therefore, CK and LDH can be used as indicators of animal welfare in slaughter cattle but cannot be used to predict the quality of meat. Bulls were the heaviest, although heifers had the highest dressing percentage than cows. Furthermore, the nanostructure of beef indicated that heifers had the best muscle fibres, sarcomere length and visible intercalated discs. The observed nanostructures of Nguni genotype are an indication of tender meat. Beef from heifers of Nguni had visible and tender meat with traces of intramuscular fat while males had shorter sarcomere length and tougher meat. Keywords: Eigenvalues, HSPA1A, glucose, meat quality, muscle fibres, nanostructure, pre-slaughter stress, road transportation, scanning electron microscope, stress biomarkers, ultimate p

    Going Beyond Counting First Authors in Author Co-citation Analysis

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    The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed

    Variations on the Author

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    “Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship

    Appropriate Similarity Measures for Author Cocitation Analysis

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    We provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis

    Dispelling the Myths Behind First-author Citation Counts

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    We conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more sophisticated methods

    Author Index

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    koamabayili/VECTRON-author-checklist: VECTRON author checklist

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    We have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
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