148 research outputs found
Network of Interactions of a Novel Plant-specific Arg/Ser-rich Protein, atRSZ33, with atSC35-like Splicing Factors
No full textArg/Ser-rich (RS) proteins play a crucial role in splicing and are implicated in splice site selection in metazoa. In plants, intron recognition seems to differ from the one in animals due to specific factor requirements. Here we describe a new plant-specific RS-rich protein, atRSZ33, with a unique domain structure consisting of an RNA recognition motif (RRM), two zinc knuckles embedded in a basic RS region, and an acidic C-terminal domain. atRSZ33 was found to be a phosphoprotein that concentrates in nuclear speckles and is predominantly present in roots and flowers. In a yeast two-hybrid screen, atRSZ33 interacted with splicing factors atSRp34/SR1, an Arabidopsis ortholog of human SF2/ASF; atRSZp21 and atRSZp22, which are similar to the human 9G8; and three novel SC35-like splicing factors termed atSCL28, atSCL30, and atSCL33/SR33. Two further members of the SCL family, namely SCL30a and the ortholog of mammalian SC35, atSC35, were also found to interact with atRSZ33. These interactions were verified by in vitro binding assays; furthermore, the transcriptional activity of atRSZ33 was found to overlap with the ones of its interacting partners. These specific interactions coupled with the many similarities of atRSZ33 to SR proteins suggest that its main activity is in spliceosome assembly. Mapping of regions necessary for protein-protein interaction between atRSZ33 and atSCL33/SR33 revealed that both zinc knuckles together with a small part of the RS and the RRM domain are required for efficient binding. However, the interacting domain is relatively small, allowing binding of additional proteins, a feature that is consistent with the proposed role of atRSZ33 in spliceosome assembly.Sergiy Lopato, Christina Forstner, Maria Kalyna, Julia Hilscher, Ulrike Langhammer, Korakod Indrapichate, Zdravko J. Lorkovic, and Andrea Bart
Precisie van lijnvolging in digitale beelden
No full textIn digital images lines can be detected using certain digital image processing techniques. The most important goal of this research is to determine the precision of two line following operators. To achieve this, a large scale aerial colour photo has been scanned. The technique which has been applied can be divided in three phases : 1. smoothing Smoothing of noise without affecting the edges in the image. The unweighted conditioned filter, the Kuwahara filter and the median filter have been used. 2. edge detection Determination of an edge image. The normal gradient, the Prewitt and the Sobel operator and the Forstner operator have been applied. 3. line following Connection of the candidate edges by using neighbourhood information. Edge relaxation and dynamic programming have been used.Civil Engineering and Geoscience
Quo vadis, CIUTI?
No full textLe présent article retrace le cheminement de la CIUTI depuis 50 ans, c’est-à-dire son développement en une organisation réputée ayant à son actif 40 membres en Europe et dans le monde. À ses débuts, la CIUTI avait certes comme but essentiel la promotion de la coopération entre ses membres (mobilité des étudiants et des enseignants, échange de connaissances), mais son autre objectif était de promouvoir la traductologie dans les universités en tant que discipline à part entière. L’auteur affirme qu’être membre de la CIUTI a une valeur symbolique qui confère, de ce fait, un capital social valorisant, sur les plans national et international, notamment en ce qui concerne le classement des instituts. En 1994, la CIUTI est devenue une association internationale régie par la loi belge ; en tant que telle, la CIUTI a voulu jouer son rôle en matière de formation afin d’influencer la politique langagière et traductionnelle européenne, en collaboration avec d’autres organisations internationales. Ses actions ont été facilitées par le fait que la CIUTI a aussi été déclarée ONG à Genève en 2003. Cependant, selon l’auteur, le développement interne de la CIUTI n’a pas suivi le même rythme que le rayonnement externe, indéniable, que cette association a connu et connaît encore en tant qu’important acteur international. Aussi, des modifications de la structure décisionnelle seront nécessaires, ainsi que la mise à disposition de moyens financiers liée à l’attribution de plus de pouvoir au Conseil de la CIUTI, de manière à permettre la mise en place d’une gestion professionnelle et efficace des affaires publiques. Une décision délicate incombe à la CIUTI : soit continuer à suivre le chemin déjà tracé et éviter toute forme de risque, soit prendre une voie nouvelle et accepter son rôle en tant qu’acteur clé dans notre ère de mondialisation.This article shows that within 50 years CIUTI has developed into a reputable organization now with 40 member institutes both within and outside of Europe. In the beginning CIUTI was devoted to promoting the cooperation between its members (mobility of students and trainers; exchange of knowledge), but was also anxious to anchor translation studies at universities as a scientific discipline in its own right. The author shows that CIUTI membership is of symbolic value and provides important social capital thus being of advantage to its members in their national and international ranking. In 1994, CIUTI became an international association (association internationale) under Belgian law and then began to play a role in education matters to influence European language and translation politics in collaboration with other international organizations. This was made easier by the fact that CIUTI was registered as a NGO in Geneva in 2003. However, according to the author of this article, the internal development of CIUTI has not kept pace with the external requirements of becoming a global player. That will require changes in its decision making structure and in the disposal of its financial resources by giving the Board (Conseil) more power to implement an appropriately efficient and professional public affairs strategy. CIUTI has a difficult decision to make, either to stay on the beaten track and avoid any risks or to forge a new future for itself and accept its role as a major global player
Bacterial Growth Kinetics under a Novel Flexible Methacrylate Dressing Serving as a Drug Delivery Vehicle for Antiseptics
No full textA flexible methacrylate powder dressing (Altrazeal®) transforms into a wound contour conforming matrix once in contact with wound exudate. We hypothesised that it may also serve as a drug delivery vehicle for antiseptics. The antimicrobial efficacy and influence on bacterial growth kinetics in combination with three antiseptics was investigated in an in vitro porcine wound model. Standardized in vitro wounds were contaminated with Staphylococcus aureus (MRSA; ATCC 33591) and divided into six groups: no dressing (negative control), methacrylate dressing alone, and combinations with application of 0.02% Polyhexamethylene Biguanide (PHMB), 0.4% PHMB, 0.1% PHMB + 0.1% betaine, 7.7 mg/mL Povidone-iodine (PVP-iodine), and 0.1% Octenidine-dihydrochloride (OCT) + 2% phenoxyethanol. Bacterial load per gram tissue was measured over five days. The highest reduction was observed with PVP-iodine at 24 h to log10 1.43 cfu/g, followed by OCT at 48 h to log10 2.41 cfu/g. Whilst 0.02% PHMB resulted in a stable bacterial load over 120 h to log10 4.00 cfu/g over 120 h, 0.1% PHMB + 0.1% betaine inhibited growth during the first 48 h, with slightly increasing bacterial numbers up to log10 5.38 cfu/g at 120 h. These results indicate that this flexible methacrylate dressing can be loaded with various antiseptics serving as drug delivery system. Depending on the selected combination, an individually shaped and controlled antibacterial effect may be achieved using the same type of wound dressing
Bóg sprawiedliwości i Bóg miłosierdzia w świetle Wj 33, 19 i Wj 34, 6-7
No full textUndertaking the theme of justice and charity of God, the author wants to show that these “attributes” of God do not exclude each other mutually. They mutually complete each other and are inseparably and existentially related to each other. Both come from God, in Him have their beginning and the source. Justice and charity are God’s gifts for us. We are their continuous recipients. If we begin to contrast justice and charity, sooner or later they will lose their sense and meaning in our life, and through this we will destroy also their beneficial character planned by God in his economy of salvation. Justice without charity will become “a cold” doctrine, and charity without justice will prove only a servile, meaningless gesture
Bóg sprawiedliwości i Bóg miłosierdzia w świetle Wj 33, 19 i Wj 34, 6-7
No full textUndertaking the theme of justice and charity of God, the author wants to show that these “attributes” of God do not exclude each other mutually. They mutually complete each other and are inseparably and existentially related to each other. Both come from God, in Him have their beginning and the source. Justice and charity are God’s gifts for us. We are their continuous recipients. If we begin to contrast justice and charity, sooner or later they will lose their sense and meaning in our life, and through this we will destroy also their beneficial character planned by God in his economy of salvation. Justice without charity will become “a cold” doctrine, and charity without justice will prove only a servile, meaningless gesture
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