927 research outputs found
Photocatalytic splitting of water on NiO/InTaO4 catalysts prepared by an innovative sol-gel method
No full textMediation of amphetamine-induced long-term depression of synaptic transmission by CB1 cannabinoid receptors in the rat amygdala.
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Acquisition of JAK2, PTPN11, and RAS mutations during disease progression in primary myelodysplastic syndrome
No full textImmunopathological effects of porcine circovirus type 2 (PCV2) on swine alveolar macrophages by in vitro inoculation
No full textYC-1 enhances the anti-tumor activity of sorafenib through inhibition of signal transducer and activator of transcription 3 (STAT3) in hepatocellular carcinoma
Full text linkBackground: Traditional systemic chemotherapy does not provide survival benefits in patients with hepatocellular carcinoma (HCC). Molecular targeted therapy shows promise for HCC treatment, however, the duration of effectiveness for targeted therapies is finite and combination therapies offer the potential for improved effectiveness. Methods: Sorafenib, a multikinase inhibitor, and YC-1, a soluble guanylyl cyclase (sGC) activator, were tested in HCC by proliferation assay, cell cycle analysis and western blot in vitro and orthotopic and ectopic HCC models in vivo. Results: In vitro, combination of sorafenib and YC-1 synergistically inhibited proliferation and colony formation of HepG2, BEL-7402 and HCCLM3 cells. The combination also induced S cell cycle arrest and apoptosis, as observed by activated PARP and caspase 8. Sorafenib and YC-1 respectively suppressed the expression of phosphorylated STAT3 (p-STAT3) (Y705) in a dose-and time-dependent manner. Combination of sorafenib and YC-1 significantly inhibited the expression of p-STAT3 (Y705) (S727), p-ERK1/2, cyclin D1 and survivin and SHP-1 activity compared with sorafenib or YC-1 used alone in all tested HCC cell lines. In vivo, sorafenib-YC-1 combination significantly suppressed the growth of HepG2 tumor xenografts with decreased cell proliferation and increased apoptosis observed by PCNA and PARP. Similar results were also confirmed in a HCCLM3 orthotopic model. There was a reduction in CD31-positive blood vessels and reduced VEGF expression, which suggested a combinational effect of sorafenib and YC-1 on angiogenesis. The reduced expression of p-STAT3, cyclin D1 and survivin was also observed with the combination of sorafenib and YC-1. Conclusions: Our data show that sorafenib-YC-1 combination is a novel potent therapeutic agent that can target the STAT3 signaling pathway to inhibit HCC tumor growth.Biochemistry & Molecular BiologyOncologySCI(E)[email protected]; [email protected]
Cultural adaptation of the young children's participation and environment measure (YC-PEM) for use by Hispanic families of young children with special health care needs (CSHCN)
Full text linkIncludes bibliographical references.2016 Summer.Culture informs the occupations in which children engage as well as how they are enacted. Hence, occupational therapists need assessments that are culturally relevant in order to deliver culturally competent practice. Current approaches to cultural adaptation of assessments present with three major limitations: (a) use of inconsistent translation process; (b) current processes assess for some, but not all, elements of cultural equivalence; and (c) limited evidence to guide decision making about whether to undertake cultural adaptation with and without language translation. To our knowledge, this is the first study to systematically develop and compare multiple versions of a culturally adapted questionnaire for potential use by a Hispanic population of young children with special health care needs (CSHCN). The purpose of this study is two-fold: (a) to examine similarities and differences of culturally adapting an occupation-centered pediatric assessment with and without translation; and (b) to examine the feasibility of developing a culturally adapted assessment with and without translation. The Young Children’s Participation and Environment Measure (YC-PEM) underwent cultural adaptation processes (i.e., language translation and cognitive testing) to establish Spanish and English pilot versions for potential use by caregivers of young CSHCN of Mexican descent. Following language translation to develop a Spanish YC-PEM pilot version, 7 caregivers (4 with Spanish as their primary language; 3 with English as their primary language) completed cognitive testing to inform decisions regarding content revisions to the YC-PEM Spanish and English pilot versions. Participant responses were content coded to established cultural equivalencies (i.e., semantic/idiomatic, item, conceptual). Coded data were then summed to draw comparisons on the number of revisions needed to achieve cultural equivalence between the two pilot versions. Feasibility was assessed according to resources required, data collection procedures, and data quality. Results suggest that a greater number of revisions are required to achieve cultural equivalence for the translated (Spanish) version of the YC-PEM. However, issues concerning conceptual equivalence were identified in both the Spanish and English versions. Feasibility results indicate that language translation processes require high resource investment, but may increase translation quality. However, use of questionnaire (i.e., paper, PDF) cognitive testing versus interview methods (e.g., phone, face-to-face) may have limited data saturation. Study results lend preliminary support to the need for and feasibility of pursuing cultural adaptation of the YC-PEM with and without language translation. Larger and more diverse samples are needed to examine the effects of acculturation status on revisions needed to achieve cultural equivalence. Also, interview methods may help improve data quality and confirm study findings
FOURIER TRANSFORM MICROWAVE SPECTRUM OF THE YC (XA) RADICAL
No full textAuthor Institution: Department of Chemistry, Department of Astronomy, and Steward Observatory, University of Arizona, Tucson, AZ 85721The pure rotational spectrum of YC (XA) in the range 4 - 40 GHz has been measured using Fourier transform microwave (FTMW) techniques. The species was produced using Discharge Assisted Laser Ablation Spectroscopy (DALAS) in a supersonic jet expansion of yttrium vapor and HCCH or CH, diluted in argon carrier gas. Three rotational transitions (N = 1 0, 2 1, and 3 2) have been recorded each exhibiting fine structure and hyperfine splittings due to the yttrium nuclear spin of I(Y) = 1/2. The data have been analyzed wtih a case (b) asymmetric top Hamiltonian, and rotational, fine, and hyperfine constants have been determined. The spectrum of this species was previously measured by PPMODR methods, and our data have refined the spectroscopic constants. Measurements of the C isotopologues are currently underway to establish a precise structure for YC
Cocarriage of distinct blaKPC-2 and blaOXA-48 plasmids in a single sequence type 11 carbapenem-resistant klebsiella pneumoniae isolate[Erratum:Antimicrobial Agents and Chemotherapy. 2019 Jun;63(6):Article number e02282-18.]
No full text[[abstract]]Volume 63, no. 6, e02282-18, 2019, https://doi.org/10.1128/AAC.02282-18. In the originally published article, one institutional affiliation was missing for the first author. The corrected affiliation line is shown above
Feeding value of high-oil corn for Taiwan country chicken
No full textThe feeding value of high-oil corn fed to Taiwan Country (TC) chicken was examined by measuring apparent metabolizable energy (AME), growth performance, sexual maturity, carcass characteristics, and plasma pigmentation. In a completely randomized design, 870 sex-intermingled one-wk-old chicks were assigned to one of 30 floor pens, 29 birds per pen, and each pen randomly assigned to one of five dietary treatments. The experiment was ended when birds were 16 wk of age. The five dietary treatments varied in main fat sources, which were corn oil (CO), high oil coin (HOC), lard (LRD), whole soybean (WSB) and yellow corn (YC), respectively. All the diets were formulated isonitrogenously, isocalorically, and of equal lysine and methionine contents except YC, in which equal amounts of YC replaced HOC. The results indicated that feed conversion in HOC was 8% higher (p<0.05) than YC whereas the calculated AME of HOC was only 3.5% to 4.0% higher than that of YC. No significant differences were observed in body weight, body weight gain, feed consumption, feed conversion ratio and ME efficiency for body weight gain among CO, HOC, LRD, and WSB. No significant differences existed in both skin and muscle pigmentation of breast among the five dietary treatments. No significance differences existed in plasma carotenoid content measured at various ages among the five dietary treatments except that birds fed with HOC had less (p<0.05) plasma carotenoids at 16 wk-old. The results indicate that if the price of high-oil corn is no more than 1.05 times that of yellow corn, the dietary cost per kg of body weight gain for TC chickens fed diets containing high-oil corn will be less, although their body weight may be lighter compared to chickens fed diets formulated with other fat sources
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