172,039 research outputs found
Program Notes Chiao-Yu Huang\ue2s Vocal Recital
No full textAbstract
This easy is the program notes of Chiao-Yu Huang\ue2s Vocal Recital held on May, 17, 2022 in the form of academic report. The works of the program were composed by five composers who belong to Classical, Romantic, and Modern periods separately. The languages of the program include Latin, Italian, German and Chinese. The content of the program includes Exsultate, jubilate (K. 165), Nehmt meinen Dank, ihr holden G\uc3\ub6nner ( K. 383) and Vorrei spiegarvi, oh Dio (K. 418) by Wolfgang Amadeus Mozart, When the Fog Rises by Nan-Chang Chien, The Color Palette of the Lover\ue2s Night by Chiung-Yu Chen, three Lieder chosen from Richard Strauss\ue2s Op. 27- C\uc3\ua4cilie, Heimliche Aufforderung, Morgen, and three arias from Gaetano Donizetti\ue2s opera L'elisir d'amore including Della crudele Isotta, Chi \uc3\ua8 mai quel matto, Prendi, per me sei libero. The discussion of this report will be focus on the compositional background, translation of the lyrics, as well as some interpretation ideas considering the music
Effect of near-ridge thermal anomalies on the subsidence of the oceanic lithosphere: Constrains from a two dimensional dynamic model
No full textDo older adults become more depressed with age in Taiwan? The role of social position and birth cohort
No full textThe Informative Content of the Net-Buy Information of Institutional Investors in the Taiwan Stock Market: A Revisit Using Conditional Analysis
No full textChiao and Lin (2004) argue that the strategies following the investment pace of security investment trust companies (SITCs) are more profitable than the market and those following foreign investors (FIs) and security dealers (SDs) in the Taiwan stock market. We reexamine their results under various market events and economic states. Employing the close-to-close returns, our results are mostly consistent with Chiao and Lin (2004) except that, during the period of economic recovery, following FIs is more profitable. However, employing the open-to-close returns and considering transaction costs, none of the strategies are profitable.Foreign investors, security investment trust companies, security dealers, conditional analysis, JEL Classification: G11, JEL Classification: G24
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Mitomycin C in highly myopic eyes - Author reply
No full textOphthalmology. 2005 Feb;112(2):208-18; discussion 219.
Mitomycin C modulation of corneal wound healing after photorefractive keratectomy in highly myopic eyes.
Gambato C, Ghirlando A, Moretto E, Busato F, Midena E.
SourceRefractive Surgery Service and Antimetabolite Therapy Research Unit, Department of Ophthalmology, University of Padova, Padova, Italy.
Abstract
PURPOSE: To evaluate the role of topical mitomycin C in corneal wound healing (CWH) after photorefractive keratectomy (PRK) in highly myopic eyes.
DESIGN: Prospective, double-masked, randomized clinical trial.
PARTICIPANTS: Seventy-two eyes of 36 patients affected by high (>7 diopters) myopia.
METHODS: In each patient, one eye was randomly assigned to PRK with intraoperative topical 0.02% mitomycin C application, and the fellow eye was treated with a placebo. Postoperatively, mitomycin C-treated eyes received artificial tears (3 times daily, tapered in 3 months), whereas the fellow eye was treated with fluorometholone sodium 2% and artificial tears (3 times daily, tapered in 3 months).
MAIN OUTCOME MEASURES: Uncorrected visual acuity (UCVA) and best-corrected visual acuity (BCVA), contrast sensitivity, manifest refraction, and biomicroscopy. Contrast sensitivity was determined using the Pelli-Robson chart. Corneal confocal microscopy documented CWH.
RESULTS: Mean follow-up was 18 months (range, 12-36). No side effects or toxic effects were documented. At 12-month follow-up examination, UCVAs (logarithm of the minimum angle of resolution) were 0.4+/-0.48 and 0.5+/-0.53 (P = .03) in mitomycin C-treated eyes and corticosteroid-treated eyes, respectively. At 1 year, corneal haze developed in 20% of corticosteroid-treated eyes, versus 0% of mitomycin C-treated eyes. At 12, 24, and 36 months, corneal confocal microscopy showed activated keratocytes and extracellular matrix significantly more evident in untreated eyes (Ps = 0.004, 0.024, and 0.046, respectively).
CONCLUSION: Topical intraoperative application of 0.02% mitomycin C can reduce haze formation in highly myopic eyes undergoing PRK.
Comment in
Ophthalmology. 2006 Feb;113(2):357; author reply 357-8
Role of Human Hepatic Cytochrome P450s in Territrem B and C Metabolism
No full text土震素為土麴菌編號23-1 (Aspergillus terreus 23-1) 經米培養，由氯仿萃取分離純化出土震素A、B、C (TRA、TRB、TRC) 三種震顫性黴菌毒素，過去研究指出土震素A可經由成熟雄性大鼠肝臟微粒體代謝生成MA1、MAX以及MA2，而雌性大鼠僅能代謝至MA1；土震素B於兩性大鼠肝臟微粒體中均可被代謝成MB1、MB2、MB3、MB4 (同土震素C)，TRC再經由氫氧化作用 (hydroxylation) 產生MC (同MB1)，但其代謝物產量則有年齡及性別上的差異性。然後由不同細胞色素P450同功酶之化學、抗體抑制劑以及supersomes確定CYP3A1/2為主要參與TRA、TRB及TRC代謝的P450酵素，其中CYP3A1只參與TRA至MA1的步驟。
土震素為早期在台灣穀倉黴米中篩選出的黴菌Aspergillus terreus 23-1菌株在米培養產生的毒素，目前已知土震素A可經由人肝臟微粒體代謝生成MA1，且證實CYP3A4為主要參與TRA代謝作用的P450酵素。爲了探討在人體肝臟中細胞色素P450同功酶參與TRB與TRC代謝作用之情形，因此本實驗採用人肝臟微粒體及以基因工程轉殖並表現人類細胞色素P450 3A4的中國倉鼠肺臟纖維母細胞 (Chinese hamster lung fibroblast) V79衍生之V79MZh3A4細胞株，作為探討對於土震素B及土震素C代謝研究的生物體外模式。
研究結果顯示：（1）人肝臟對於TRB之代謝作用，可經由4β-C hydroxylation生成MB2及O-demethylation生成MB4，且MB2之產量均大於MB4；對於TRC之代謝作用，則經由4β-C hydroxylation代謝生成MC。人類肝臟對於TRB或是TRC的代謝作用，和人類之性別及年齡均無顯著相關性。（2）藉由化學抑制劑、抗體抑制劑、V79MZh3A4細胞株及以不同細胞色素P450同功酶之cDNA利用baculovirus轉植入insect cell (BTI-TN-5B1-4) 之supersomes的實驗進一步證實CYP3A4/5為主要協助人體對於TRB與TRC進行代謝作用的P450同功酶。（3）在V79MZh3A4細胞株對TRB、TRC和testosterone相互競爭抑制之作用探討中得知，testosterone對TRB代謝生成MB2過程的影響為競爭性抑制作用 (competitive inhibition)，testosterone對TRB代謝生成MB4過程的影響為未競爭抑制作用 (uncompetitive inhibition)；相對TRB對testosterone代謝生成6β-hydroxytestosterone過程的影響為mixed inhibition。Testosterone與TRC之間則互相為混合型抑制作用 (mixed inhibition)。（4）在探討人肝臟微粒體中CYP3A4/5對於TRB 4β-C hydroxylation及TRB O-demethylation之參與情形的實驗結果得知，由基因重組系統及人肝臟微粒體所求得的CLCYP3A4及CLCYP3A5值，可更加證實了在人肝臟微粒體中，CYP3A4是趨向參與TRB 4β-C hydroxylation生成MB2過程之P450酵素，而CYP3A5是趨向參與TRB O-demethylation生成MB4過程之P450酵素。Territrem A, B and C, the structure related tremogenic mycotoxins isolated from the chloroform extracts of rice culture of Aspergillus terreus 23-1. The previous study on metabolism of Territrem A (TRA) by liver microsome from Wistar rats showed that three metabolites, MA1, MAX and MA2 were formed in male rats, but only one metabolite, MA1 in female rats. The studies were further carried with specific chemical and antibody inhibitors for CYP isoforms and the sypersomes expressed with specific type of CYP isoforms. The results indicated that CYP3A1, which is dominated in female rat liver, plays a mean role in metabolic pathway from TRA to MA1 and that CYP3A2, which is dominated in male rat liver, plays the mean role in metabolic pathway of TRA to MA1, MAX and MA2.
On the other hand, four metabolites, such as MB1, MB2, MB3 and MB4 (which structure is the same as Territrem C (TRC) ) were formed from Territrem B (TRB) and one metabolite, MC (which structure is the same as MB4) from Territrem C respectively by liver microsome from each of male and female rats.
In order to elucidate the role of human hepatic CYP isoforms in TRB and TRC, the experiments were performed with several enzyme sources such as from different age and sex, V79MZh3A4 cell line derived from Chinese hamster, in which human CYP3A4 were expressed, and several supersomes having enzyme or different tyoe of CYP isoforms.
The following are the resultes obtained： (1) In human liver microsome, MB2, and MB4 were main products from TRB and MC from TRC. There were no age and gender difference in ability to metabolize TRB and TRC. (2) CYP3A4/5 had the major role in metabolism of TRB and TRC. (3) The rate of production of MB2 from TRB or MC from TRC were competitively inhibited by the present of testosterone. However the rate of the production of 6β-hydroxytestosterone from testosterone were inhibited by the present of TRB or TRC in mixed type of inhibition (non competitive and non uncompetitive inhibition). The rate of the production of MB4 from TRB was also inhibited by the present of testosterone in mixed type of inhibition. (4) It suggested that CYP3A4 had the major role in 4β-C hydroxylation of TRB, and CYP3A5 had the major role in O-demethylation of TRB.圖表目錄 Ⅰ
縮寫表 Ⅴ
中文摘要 Ⅵ
英文摘要 Ⅸ
第一章 绪論 1
第二章 實驗材料與方法 14
第三章 實驗結果 44
第四章 討論 61
第五章 參考文獻 73
附表 83
附圖 9
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
Regulation of the gene expression of FASTK and HRG by the nonstructural protein 4B of hepatitis C virus
No full textC型肝炎病毒 (Hepatitis C Virus，HCV) 是造成非A非B型肝炎的主要致病因子，屬於黃病毒科 (Flaviviridae)，具外套膜，為一單股正向RNA病毒。根據本實驗室對HCV subgenomic replicon細胞株和其母細胞株Huh7進行比較之cDNA microarray分析，以及其他實驗室比較肝癌病人癌細胞和正常細胞基因表現差異的研究結果，顯示在HCV感染時一些細胞基因的表現可能會受到調控，例如：SAA、DUSP1、GRB14、VEGF-A、integrin、FASTK、H-ras、HRG、SULT2A1等。為確認此調節作用並釐清造成此現象的病毒蛋白質，本研究利用DNA轉染的方式，分別將HCV非結構性蛋白質之表現質體送至培養細胞中表現，並以即時定量聚合酵素鏈鎖反應 (real-time quantitative PCR) 分析不同病毒蛋白質對於細胞基因表現的影響。結果發現NS4B會造成FASTK與HRG表現量的下降。NS4B為病毒非結構性蛋白質之一，分子量 27 kD，具疏水性，其在病毒生活史的功能目前尚不清楚，但有研究指出NS4B對於NS5A的磷酸化，和病毒RNA複製是重要的；另外NS4B具有GTPase活性，有抑制宿主細胞轉譯的能力，也會與Ha-ras基因共同造成細胞轉型，可能在HCV的致病機轉中扮演重要角色。已知FASTK為一survival protein，因此嘗試分析NS4B對細胞存活的影響，以西方墨點法偵測細胞中caspase3的含量，發現在受到anti-Fas抗體刺激誘導細胞凋亡的293細胞中，NS4B會使細胞凋亡的情形更為嚴重。不過在nucleosome ELISA assay和flow cytometry的分析上，NS4B對細胞凋亡的影響則不明顯。細胞凋亡為幫助病毒顆粒散播的方式之一，NS4B是否藉由細胞凋亡調節C型肝炎病毒的傳播過程，則需進ㄧ步的釐清。另一方面，HRG為一含量豐富的血漿蛋白質，主要在肝臟細胞中表現，本研究發現在表現NS4B的Huh7細胞中，HRG的mRNA量和蛋白質量均有下降的情形。將NS4B表現質體與帶有長度約450 bp的HRG promoter之luciferase reporter進行共同轉染時，發現NS4B並不會影響luciferase的活性，顯示NS4B不會藉由此段HRG promoter調控HRG mRNA的合成。其調控機制仍有待進一步的研究。Hepatitis C virus (HCV) is the major infectious agent leading to non-A, non-B hepatitis. HCV belongs to the family Flaviviridae and is an enveloped, single-stranded RNA virus. Previously, a cDNA microarray analysis with HCV subgenomic replicon and patients parental Huh7 cells was performed in our lab to identify differentially expressed genes. According to the results and data from RT-PCR analysis of hepatocytes of HCC patients by another group, it is likely that HCV proteins are involved in the regulation of expression of several genes including SAA, DUSP1, GRB14, VEGF-A, integrin, FASTK, H-ras, HRG, and SULT2A1. In this study, cultured cells were transfected with expression plasmids of HCV nonstructural proteins. RT-PCR was applied to analyze the effects of the viral proteins on the expression of the cellular genes. NS4B down-regulated the expression of both FASTK and HRG mRNA. Nonstructural protein 4B (NS4B) is a relatively hydrophobic 27 kDa protein of unknown function. Nevertheless, NS4B was demonstrated to be important for the NS5A hyperphosphorylation and replication of HCV. In addition, NS4B has a GTPase activity. It inhibits translation of host cells, and causes malignant transformation in cooperation with the Ha-ras gene. Therefore, NS4B may play an important role in the pathogenicity of HCV. FASTK was shown to be a survival protein. To understand possible effects of the HCV NS4B protein on cellular survivability, western blot analysis was performed to detect the level of caspase3 in both anti-Fas antibody-treated and untreated 293 cells. Although NS4B seems to increase the severity of cell apoptosis, only limited effect was detected by nucleosome ELISA assay and flow cytometry. Apoptosis is one of gateways for viral particle spreads, the role of NS4B involved in the apoptosis and in the process of viral diffusion need to be further elucidated. On the other hand, HRG is an abundant plasma protein. It expresses mainly in the liver tissue. In this study, NS4B was found to down-regulate HRG both at the RNA and protein levels. When NS4B expression plasmid was cotransfected with a luciferase reporter carrying a 450 bp HRG promoter, no effect on the luciferase activity was detected. These results suggested that the regulatory effect of NS4B on HRG is not through a direct binding of the NS4B protein to the 450 bp promoter sequences.目錄………………………………………………………1
中文摘要.……………………………………………….2
英文摘要…………………………………………………3
緒論………………………………………………………5
研究方向………………………………………………..18
材料與方法……………………………………………..19
實驗結果………………………………………………..32
討論……………………………………………………..38
圖表……………………………………………………..42
參考文獻………………………………………………..6
A comprehensive 3D seismic velocity model for the eastern Taiwan-southernmost Ryukyu regions.
No full text- …
