1,720,966 research outputs found
Effects of Streptococcus Thermophilus Bacteria on rat gene expression profiles
No full textIn questo studio abbiamo analizzato l'impatto dellethermophiluds Streptococcus sull'epitelio del colon di ratto. Dopo la generazione del modello di ratto axenico e inoculato con S. thermophilus abbiamo investigato l'interazione tra il batterio e epithelo del colon di ratto. Dopo questo studio integrativo abbiamo analizzato l’espressione genica del colon usando due diversi approcci:
Ibridazione sottrattiva
I trascritti ottenuti dopo il sequenziamento dei cloni ottenuti con la SSH sono stati raggruppati in diversi categorie funzionali: arresto del ciclo cellulare e induzione del differenziamento, comunicazione cellulare e binding . due geni candidati sono stati privilegiati, krupel like fattore 4 e 14-3-3σ. Questi geni candidati sono stati analizzati mediante RT-PCR, qRT-PCR e Western Blot in animali mono-associati e in animali germ-free. I test hanno confermato che l’espressione dei geni candidati aumenta in presenza di S. thermophilus.
Microarray Analysis.
L’spressione genica è stata misurata per due gruppi di animali: i) ratti privi di germi, ii) ratti mono-associati con il ceppo LMD9 di Streptococcus thermophilus. I risultati delle analisi dei dati di microarray indicano che Streptococcus thermophilus influenza notevolmente l'espressione genica nelle cellule epiteliali del colon.In this study we have investigated the impact of Streptococcus thermophiluds on the rat colonic epithelium. After generation of the model axenic rat and inoculated with S. thermophilus we have investigated the interplay between bacteria and host colon. Colonic epithelium gene expression was investigated also, with two different approaches:
Suppressive Subtractive Hybridization.
The subtraction library was prepared subtracting mRNA between epithelial cells from colonic mono-associated rats and germ-free rats. The transcripts generated by SSH were grouped into divers Functional groups: cell-cycle arrest and induction of differentiation; cell-communication and binding. Tow candidates genes were privileged, krupel like factor 4 and 14-3-3σ. These candidate genes were tested by RT-PCR, qRT-PCR and Western blot in mono-associated animals and in germ-free animals. The tests confirmed that candidate genes increase their expression in the presence of S. thermophilus.
Microarray analysis.
Gene expression was measured in tow groups of animals: i) germ-free rats; ii) mono-associated rats inoculated with LMD9 strain of Streptococcus thermophilus. The results of microarray analysis data show that Streptococcus thermophilus remarkably affected gene expression in the colonic epithelial cells. Streptococcus thermophilus enhanced the expression of genes involved in different pathways in the host, compared to the gem free group
Single nucleotide polymorphisms in collagen genes and association with skin quality trait
No full textEffects of Streptococcus Thermophilus Bacteria on rat gene expression profiles
No full textIn questo studio abbiamo analizzato l'impatto dellethermophiluds Streptococcus sull'epitelio del colon di ratto. Dopo la generazione del modello di ratto axenico e inoculato con S. thermophilus abbiamo investigato l'interazione tra il batterio e epithelo del colon di ratto. Dopo questo studio integrativo abbiamo analizzato l’espressione genica del colon usando due diversi approcci:
Ibridazione sottrattiva
I trascritti ottenuti dopo il sequenziamento dei cloni ottenuti con la SSH sono stati raggruppati in diversi categorie funzionali: arresto del ciclo cellulare e induzione del differenziamento, comunicazione cellulare e binding . due geni candidati sono stati privilegiati, krupel like fattore 4 e 14-3-3σ. Questi geni candidati sono stati analizzati mediante RT-PCR, qRT-PCR e Western Blot in animali mono-associati e in animali germ-free. I test hanno confermato che l’espressione dei geni candidati aumenta in presenza di S. thermophilus.
Microarray Analysis.
L’spressione genica è stata misurata per due gruppi di animali: i) ratti privi di germi, ii) ratti mono-associati con il ceppo LMD9 di Streptococcus thermophilus. I risultati delle analisi dei dati di microarray indicano che Streptococcus thermophilus influenza notevolmente l'espressione genica nelle cellule epiteliali del colon.In this study we have investigated the impact of Streptococcus thermophiluds on the rat colonic epithelium. After generation of the model axenic rat and inoculated with S. thermophilus we have investigated the interplay between bacteria and host colon. Colonic epithelium gene expression was investigated also, with two different approaches:
Suppressive Subtractive Hybridization.
The subtraction library was prepared subtracting mRNA between epithelial cells from colonic mono-associated rats and germ-free rats. The transcripts generated by SSH were grouped into divers Functional groups: cell-cycle arrest and induction of differentiation; cell-communication and binding. Tow candidates genes were privileged, krupel like factor 4 and 14-3-3σ. These candidate genes were tested by RT-PCR, qRT-PCR and Western blot in mono-associated animals and in germ-free animals. The tests confirmed that candidate genes increase their expression in the presence of S. thermophilus.
Microarray analysis.
Gene expression was measured in tow groups of animals: i) germ-free rats; ii) mono-associated rats inoculated with LMD9 strain of Streptococcus thermophilus. The results of microarray analysis data show that Streptococcus thermophilus remarkably affected gene expression in the colonic epithelial cells. Streptococcus thermophilus enhanced the expression of genes involved in different pathways in the host, compared to the gem free group
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Nutrigenomics: a high fat diet influences gut gene expression in a mouse model
No full textA high fat level in a diet can cause local gut inflammation and
leads to metabolic abnormalities, such as obesity and insulin
resistance. This study aimed to investigate the effect of a high
fat diet in C57BL/6 mice. Twenty-seven mice were divided into
five groups. The experiment started at weaning (T0). Five mice
were sacrificed at T0 (n=5), while high-fat-fed mice (n=11) and
control-fed mice (n=11) were sacrificed after one (T1, n=5 for
each treatment) and two weeks (T2, n=6 for each treatment) of
such dietary regimens. Even though neither blood nor histological
analysis gave evidence of a clear inflammatory state in the
caecum after 1 or 2 weeks of treatment, we used 90K
Combimatrix microarray technology to check if the expression
level of genes was changed. A total of 29,435 probes in triplicates
were considered, representing all the available mouse genes in
database. After hybridization and scanning, the Cy5 signal intensities
were analyzed using limma package from Bioconductor.
Only 7 genes were detected differentially expressed between
control and high fat diet mice, out of the whole transcriptome (qvalue
≤0.05), with a range of log fold change between -2.06 and
1.22. Among statistically significant genes 4 were successfully
validated by real time PCR. In conclusion, genes differentially
expressed after two weeks of treatment all shared an involvement
in the regulatory pathway of the circadian clock system,
which was recently shown to affect lipid metabolism and inflammatory
processes
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
- …
