13,950 research outputs found
Hepatitis C virus NS5A protein down-regulates the expression of spindle gene Aspm through PKR-p38 signaling pathway.
No full textElectrophysiologic evaluation of neuromuscular functions during limb lengthening by callus distraction
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The IFN-?-induced aminopeptidase, ERAP1, determines the length of antigenic peptides by monitoring distant C-terminal residues
No full textZebrafish peptidoglycan recognition protein SC (zfPGRP-SC) mediates multiple intracellular signaling pathways
No full textInsect PGRPs can function as bacterial recognition molecules triggering proteolytic and/or signal transduction pathways, with the resultant production of antimicrobial peptides. To explore if zebrafish peptidoglycan recognition protein SC (zfPGRP-SC) has such effects, RNA interference (siRNA) and high-density oligonucleotide microarray analysis were used to identify differentially expressed genes regulated by zfPGRP-SC. The mRNA levels for a set of genes involved in Toll-like receptor signaling pathway, such as TLRs, SARM, MyD88, TRAF6 and nuclear factor (NF)-kappa B2 (p100/p52), were examined by quantitative RT-PCR (QT-PCR). The results from the arrays and QT-PCR showed that the expression of 133 genes was involved in signal transduction pathways, which included Toll-like receptor signaling, Wnt signaling, BMP signaling, insulin receptor signaling, TGF-beta signaling, GPCR signaling, small GTPase signaling, second-messenger-mediated signaling, MAPK signaling, JAK/STAT signaling, apoptosis and anti-apoptosis signaling and other signaling cascades. These signaling pathways may connect with each other to form a complex network to regulate not just immune responses but also other processes such as development and apoptosis. When transiently over-expressed in HEK293T cells, zfPGRP-SC inhibited NF-kappa B activity with and without lipopolysacharide (LPS) stimulation. (C) 2008 Elsevier Ltd. All rights reserved.Insect PGRPs can function as bacterial recognition molecules triggering proteolytic and/or signal transduction pathways, with the resultant production of antimicrobial peptides. To explore if zebrafish peptidoglycan recognition protein SC (zfPGRP-SC) has such effects, RNA interference (siRNA) and high-density oligonucleotide microarray analysis were used to identify differentially expressed genes regulated by zfPGRP-SC. The mRNA levels for a set of genes involved in Toll-like receptor signaling pathway, such as TLRs, SARM, MyD88, TRAF6 and nuclear factor (NF)-kappa B2 (p100/p52), were examined by quantitative RT-PCR (QT-PCR). The results from the arrays and QT-PCR showed that the expression of 133 genes was involved in signal transduction pathways, which included Toll-like receptor signaling, Wnt signaling, BMP signaling, insulin receptor signaling, TGF-beta signaling, GPCR signaling, small GTPase signaling, second-messenger-mediated signaling, MAPK signaling, JAK/STAT signaling, apoptosis and anti-apoptosis signaling and other signaling cascades. These signaling pathways may connect with each other to form a complex network to regulate not just immune responses but also other processes such as development and apoptosis. When transiently over-expressed in HEK293T cells, zfPGRP-SC inhibited NF-kappa B activity with and without lipopolysacharide (LPS) stimulation. (C) 2008 Elsevier Ltd. All rights reserved
Biodegradation potential of MTBE and BTEX under aerobic, nitrate reducing, and methanogenic conditions at a gasoline-contaminated site
No full text[[abstract]]In this study, a gasoline-contaminated site was selected to evaluate the biodegrading potential of methyl tert-butyl ether (MTBE) and benzene (B), toluene (T), ethylbenzene (E) and xylenes (X), collectively known as BTEX under aerobic, nitrate reducing, and methanogenic conditions at the site. Results show that in situ microorganisms had capabilities of degrading MTBE and BTEX using MTBE and BTEX as major carbon sources under aerobic condition. Under conditions of nitrate reduction and methanogenesis, BETX could be decomposed by in situ microorganism; but MTBE was not decomposed. The decomposition of BTEX under anaerobic condition was much slower than aerobic condition. Benzene had the slowest degrading rate. In addition, BTEX was decomposed faster and more completely under methanogenic condition than nitrate reducing condition. The on-site activated sludge system is effective in decomposing BTEX but not effective in decomposing MTBE. Results of this study reveal that the enhanced aerobic bioremediation method is more feasible for renovating this site. Injecting air, pure oxygen or oxygen-releasing chemicals will raise the removal rate of pollutants. Results of this study will be helpful in designing a practical system for bioremediation of this and other similar sites.[[note]]SC
Pure primary squamous cell carcinoma of the ovary: a case report and review of the literature.
No full textComparison of clinical manifestations and outcome of community-acquired bloodstream infections among the oldest old, elderly, and adult patients.
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Estudo hidrossedimentológico da bacia hidrográfica do Rio Negrinho - SC com o modelo SWAT
Full text linkDissertação (mestrado) - Universidade Federal de Santa Catarina, Centro Tecnológico. Programa de Pós-Graduação em Engenharia AmbientalO município de Rio Negrinho vem sofrendo com alteração da quantidade e qualidade da água devido a substituição da mata nativa por reflorestamento de pinus e acelerada urbanização. Visto isso, o presente estudo teve como objetivo analisar espacialmente os processos hidrossedimentológicos na bacia do Rio Negrinho - SC através do modelo SWAT. Foram realizado o monitoramento hidrossedimentológico, formação de banco de dados espaciais e tabulares, determinação das sub-bacias, calibração e validação do modelo, análises estatísticas, avaliação espacial da produção de água e de sedimentos e verificação da tolerância de perda de solos nas sub-bacias. Os dados simulados pelo modelo foram comparados com os dados observados de vazão em dois pontos de monitoramento da bacia e os dados de concentração de sedimento em um ponto da bacia. O desempenho do modelo foi analisado a partir do NASH (coeficiente de Nash-Sutcliffe), R2 (coeficiente de correlação), Erro médio (EM) e CMR (Coeficiente de massa residual). Os valores de NASH variaram de 0,48 a 0,61 para dados de vazão e 0,43 para dados de sedimento. Analisando o balanço hídrico da bacia o coeficiente de variação da precipitação, vazão e evapotranspiração mensal foi de 23%, 20% e 30% respectivamente. Já o armazenamento de água no solo variou muito pouco ao longo do ano, apresentando coeficiente de variação de 8,7%. Comparada a tolerância de perda de solos com os dados de produção de sedimento da bacia foi possível evidenciar que 11% da área da bacia apresentaram produção de sedimento maior que a tolerância.The Rio Negrinho city has been suffering from the quantitative and qualitative modification of water due to replacement of native forests by pine tree reforestation and accelerated urbanization. The objective of the present study was, therefore, to spatially analyze hydrosedimentological processes in the Negrinho river basin # SC through the SWAT model. Hydrosedimentological monitoring, construction of spatial databases and table, determination of sub-basins, the model calibration and validation, statistical analysis, spatial assessment of water and sediment yield and verification of soil loss tolerance in each sub-basin were carried out. The values simulated with the model were compared with the observed data of discharge at two monitoration points and of sediment concentration at one point in the basin. The model performance was evaluated with the coefficient of Nash-Sutcliffe (NASH), the correlation coefficient (R2), the average error (MS) and coefficient of residual mass (CRM). The NASH values were from 0.48 to 0.61 for the discharge data and 0.43 for the sediment data. Analyzing the water balance of the basin the coefficient of variation of the monthly precipitation, evapotranspiration and flow were 23%, 20% and 30%, respectively. The water storage in the soil varied little throughout the year, showing its coefficient of variation of 8.7%. Comparing to soil loss tolerance with the sediment production data, it was shown that 11% of the basin area would have more sediment production than the tolerated value
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