2,703 research outputs found
La Trobe eBureau author kit
No full textAuthor kit for La Trobe academics aiming to publish open educational resources with the La Trobe eBureau.
Includes:
* expression of interest form
* author proposal template
* author copyright agreement
* overview of eBureau publishing process
https://library.latrobe.edu.au/ebureau/ </p
G-quadruplexes: Kinetic stability and effects on the c-KIT promoter
No full textIn addition to the famous Watson & Crick model for B-form duplex DNA, guanine-rich DNA sequences can self-assemble under certain conditions to form a four-stranded structure known as a G-quadruplex. G-quadruplexes are composed of stacks of Gquartets, in which four guanines are arranged in a square planar array, interacting via eight hydrogen bonds. Monovalent cations especially K+ and Na+ but not Li+ stabilize this structure by binding with the central carbonyl O6 atoms. Bioinformatic databases have revealed potential quadruplex-forming sequences throughout the genome and tandem repeats of guanines are found to accumulate upstream of the transcription initiation site of several proto-oncogenes. The promoter region of the c-kit proto-oncogene contains two potential quadruplex forming sequences. The first part of this work focuses on understanding how the c-kit promoter is regulated by potential G-quadruplex forming structures. We have incorporated 165 base pairs of the c-kit promoter region into a luciferase reporter vector and have constructed several mutant variants of this sequence. Determining the level of luciferase expression of these constructed vectors in HeLa and HCT 116 cells have allowed us to elucidate the effect of quadruplex formation on gene expression. Our results reveal that a decrease in gene expression level is observed from the constructed vectors that carry a very stable quadruplex-forming sequence. In genomic DNA, these putative quadruplex-forming G-rich sequences are normally base paired with their complementary C-rich strands to generate duplex DNA. Structural transitions of B-form DNA (duplex) to non-B-form DNA (quadruplex) require local melting, which is facilitated by negative superhelical tension. We have examined in vitro the effect of DNA supercoiling on the reaction of the c-kit promoter (and some variants of the natural sequences) with three chemical probes KMnO4, DEPC, and DMS. The results demonstrated that negative superhelicity did not significantly affect the formation of G-quadruplex. For the first time, we have used two-dimensional agarose gel electrophoresis to probe topology-dependent structural transitions in the c-kit promoter and some of its modified versions. Our results showed that the constructed vectors that carried the very stable quadruplex-forming sequence undergo unusual structural transition. Finally, we have used a gel based assay to understand the dynamic equilibrium between quadruplex and duplex DNA under defined conditions. The results show that at elevated temperatures, the formation of duplex DNA with these G-rich sequences is kinetically reversible and we have measured the rate at which the duplex strand exchanges with single-stranded DNA. The formation of both quadruplex and duplex DNA are cation and concentration-dependent
Result of Author Kit for our SC20 paper submission
No full text<p>Result of Author Kit for our SC20 paper submission</p>
Memories of Kitchen and Diet Kit Album - page 5
No full textPage 5 - Chef. Mr. Chambers – Photograph, 3 x 2 in., B&W.
Dietician Miss Barton-Stuckey 1927 – Photograph, 3 x 2 1/8 in., B&W
An Open Book : The Redemption of Story-Kit Pearson
No full textKit Pearson is the author of over thirteen books for children, including middle grade novels in all genres, short stories, picture books, and non-fiction. Her books have been published in Canada in English and French, in the U.S., Australia, New Zealand, Japan, the Netherlands, Germany, Great Britain, France, China, and Korea. Her books have been awarded such honours as the Canadian Governor General’s Literary Award for Children’s Literature. She has received seventeen awards for her writing, including the B.C. Lieutenant Governor’s Award for Literary Excellence in 2014.
Kit was born in Edmonton, Alberta in 1947 and grew up there and in Vancouver, B.C. She received her B.A. from the University of Alberta, her M.L.S. from U.B.C.’s School of Library, Archival and Information Studies, and her M.A. from the Simmons College Center for the Study of Children’s Literature in Boston. She worked for ten years as a children’s librarian in Ontario and B.C., and is now a full-time writer living in Victoria. For more information see www.kitpearson.comAlumniUnreviewedOthe
KIT somatic mutations and immunohistochemical expression in canine oral melanoma
No full textCanine oral melanoma (COM) is an aggressive neoplasm with a low response to therapies, sharing similarities with human mucosal melanomas. In the latter, significant alterations of the proto-oncogene KIT have been shown, while in COMs only its exon 11 has been adequately investigated. In this study, 14 formalin-fixed, paraffin-embedded COMs were selected considering the following inclusion criteria: unequivocal diagnosis, presence of healthy tissue, and a known amplification status of the gene KIT (seven samples affected and seven non-affected by amplification). The DNA was extracted and KIT target exons 13, 17, and 18 were amplified by PCR and sequenced. Immunohistochemistry (IHC) for KIT and Ki67 was performed, and a quantitative index was calculated for each protein. PCR amplification and sequencing was successful in 97.62% of cases, and no single nucleotide polymorphism (SNP) was detected in any of the exons examined, similarly to exon 11 in other studies. The immunolabeling of KIT was positive in 84.6% of the samples with a mean value of 3.1 cells in positive cases, yet there was no correlation with aberration status. Our findings confirm the hypothesis that SNPs are not a frequent event in KIT activation in COMs, with the pathway activation relying mainly on amplification
Senior tool kit
No full textabstract: In an effort to educate older adults we have created this tool kit. Seniors are at risk to become victims of theft, fraud, and are particularly vulnerable to scams. This kit will help you have the tools needed to be well-informed because you could encounter a scam on the phone, through the mail, via computer or even when someone you do not know knocks on your door with an offer that may be too good to be true
Academic authorship: who, why and in what order?
No full textWe are frequently asked by our colleagues and students for advice on authorship for scientific articles. This short paper outlines some of the issues that we have experienced and the advice we usually provide. This editorial follows on from our work on submitting a paper1 and also on writing an academic paper for publication.2 We should like to start by noting that, in our view, there exist two separate, but related issues: (a) authorship and (b) order of authors. The issue of authorship centres on the notion of who can be an author, who should be an author and who definitely should not be an author, and this is partly discipline specific. The second issue, the order of authors, is usually dictated by the academic tradition from which the work comes. One can immediately envisage disagreements within a multi-disciplinary team of researchers where members of the team may have different approaches to authorship order
Transcriptional profile of c-kit positive cardiac stem-progenitor cells (c-kitpos eCSCs) isolated from the four chambers of the adult human heart
No full textIntroduction: Our findings and those of others show that the adult myocardium, including human, harbours a population of resident (endogenous) cardiac stem cells (eCSCs). They express the stem cell factor receptor c-kit, are distributed throughout the myocardium, are clonogenic, self-renewing and multi-potent, in that they differentiate into the 3 main cardiac lineages; cardiomyocytes, smooth muscle and endothelial cells in vitro and in vivo. The objective of this study is to determine whether c-kitpos eCSCs isolated from the different cardiac chambers have a distinct transcriptional profile depending on the chamber of origin.
Methods: Pieces of myocardium have been obtained from all the 4 chambers of the adult human heart. All patients were fully consented before undergone open heart surgery. They were suffered of various cardiac pathologies such as ischemic heart disease, aortic, mitral and tricuspid valve insufficiency or stenosis, and various aortic pathologies. Ethical approval for these procedures has been given by NREC (08/H1306/91).c-kitpos eCSCs were isolated by enzymatic digestion and purified by Magnetic Activated Cell Sorting (MACS) from samples taken from the right and left atria (RA, LA), right and left ventricle (RV, LV) of the adult human heart. mRNA was isolated using Qiagen® mRNA kit, and reverse transcribed using first strand cDNA synthesis with random hexamers. qRT-PCR was performed using SYBR Green on a MyIQ thermocycler Bio-Rad® of specific genes representative of the primary and secondary heart field, and the developmental program of their chamber of origin.
Results: c-kitpos eCSCs isolated from 15 human samples (5LA, 1RV, 4LV, 5RA) were processed. c-kitpos eCSCs are distributed throughout the human myocardium and in all 4 chambers of the heart. Transmitted light microscopic observations of c-kitpos eCSCs revealed that the c-kitpos cells from the human biopsies were generally small and rounded, consistent with a stable c-kitpos eCSCs phenotype, regardless of the chamber of origin. The eCSCs c-kitpos cells could be cultured under hypoxic conditions between 7 and 12 days to attain full confluency. Expression of transcripts for c-kit, Foxh1, Hand1, Hand2, Pitx2, Tbx5, Tbx20, Hrt1, Hrt2, Fgf8, Fgf10, and Isl1 were found at differential levels in c-kitpos CSCs isolated from the four cardiac chambers.
Conclusion: This study is the first to show that c-kitpos eCSCs derived from human adult cardiac samples, do not appear to have a ‘chamber-specific’ transcript footprint, and are therefore potentially interchangeable between cardiac chambers, raising the potential of their therapeutic application
Evaluation of a nationally disseminated self-help intervention for smoking cessation ('Quit Kit')
No full textInternational audienceObjective: To assess the extent of uptake and impact of a nationally disseminated self-help intervention for smoking cessation ('Quit Kit'). Methods: The kit contained practical tools for supporting quit attempts. Of 480,000 individuals receiving the kit, telephone interviews were conducted with 2347 randomly selected individuals. Interviews assessed the impact of the kit on smoking behaviours and on attitudes to the intervention and to health service support. Results: The majority of interviewees reported the kit as being helpful for stopping smoking (61%) and agreed that, having received the kit, they would be more likely to consider the National Health Service for help with quitting (84%). Younger interviewees were significantly more likely to report the kit as helpful, to say they would recommend it to others and to agree that it increased their confidence for quitting (all p<0.001). As a result of receiving the kit, 29%, 17% and 11% of interviewees, respectively, reported visiting their doctor, pharmacist or stop smoking service for help with quitting. The kit was reported to have triggered a quit attempt among around half (57%) of those receiving it. When only including those who had received the kit at least one month prior to interview, 26.5% (126/475) of those attempting to quit reported remaining completely abstinent from smoking for at least a month. Conclusions: The findings suggest that distributing a self-help intervention for smoking cessation at a national level may be successful in terms of uptake of the intervention, triggering quit attempts and aiding smoking cessation
- …
