16,516 research outputs found
Differential stability of 2'F-ANA*RNA and ANA*RNA hybrid duplexes: roles of structure, pseudohydrogen bonding, hydration, ion uptake and flexibility
No full text14 pags., 7 figs., 3 tabs.Hybrids of RNA with arabinonucleic acids 2′F-ANA and ANA have very similar structures but strikingly different thermal stabilities. We now present a thorough study combining NMR and other biophysical methods together with state-of-the-art theoretical calculations on a fully modified 10-mer hybrid duplex. Comparison between the solution structure of 2′F-ANA•RNA and ANA•RNA hybrids indicates that the increased binding affinity of 2′F-ANA is related to several subtle differences, most importantly a favorable pseudohydrogen bond (2′F-purine H8) which contrasts with unfavorable 2′-OH-nucleobase steric interactions in the case of ANA. While both 2′F-ANA and ANA strands maintained conformations in the southern/eastern sugar pucker range, the 2′F-ANA strand's structure was more compatible with the A-like structure of a hybrid duplex. No dramatic differences are found in terms of relative hydration for the two hybrids, but the ANA•RNA duplex showed lower uptake of counterions than its 2′F-ANA•RNA counterpart. Finally, while the two hybrid duplexes are of similar rigidities, 2′F-ANA single strands may be more suitably preorganized for duplex formation. Thus the dramatically increased stability of 2′F-ANA•RNA and ANA•RNA duplexes is caused by differencesin at least four areas, of which structure and pseudohydrogen bonding are the most important. © The Author(s) 2010. Published by Oxford University Press.Spanish Ministerio de Ciencia e Innovacion (grants
CTQ2007-68014-C02-02 to CG and BIO2009-10964 to
MO); Fundacion Marcelino Botin (grant to MO);
Canadian Institutes for Health Research (grant to
M.J.D.); Natural Sciences and Engineering Research
Council of Canada (postgraduate scholarship to
J.K.W.). Funding for open access charge: Canadian
Institutes for Health Research
Evaluating the behaviour of curcumin nanoemulsions and multilayer nanoemulsions during dynamic in vitro digestion
No full textNanoemulsions can be used to improve the bioaccessibility of lipophilic bioactive compounds, such as curcumin, being their behaviour highly influenced by their interfacial properties. The effect of chitosan and alginate layers deposition on curcumin nanoemulsions behaviour during in vitro digestion was evaluated using a dynamic gastrointestinal system. Results showed that polyelectrolyte layers deposition improved curcumin antioxidant capacity during in vitro digestion. In addition, multilayer nanoemulsions showed a better control of the rate and extent of lipid digestibility by decreasing free fatty acids release, compared to uncoated nanoemulsions. However, a lower curcumin bioaccessibility was observed for multilayer nanoemulsions. Although cytotoxicity assays revealed that both nanosystems are toxic due to the use of sodium dodecyl sulphate (SDS), nanosystems were 3.3-fold less toxic than SDS itself.
This study showed that multilayer nanoemulsions could be used to increase satiety by retarding lipid digestion, which can be important for functional foods development for combating obesity.The authors Hélder D. Silva, Ana C. Pinheiro and Miguel A. Cerqueira (SFRH/BD/81288/2011, SFRH/BPD/101181/2014 and SFRH/BPD/72753/2010, respectively)aretherecipientsofafellowship from the Fundação para a Ciência e Tecnologia (FCT, Portugal). This work was supported by Portuguese Foundation for Science and Technology (FCT) under the scope of the Project PTDC/AGR-TEC/5215/2014, of the strategic funding of UID/BIO/04469/2013 Unit and COMPETE 2020 (POCI-01-0145-FEDER-006684), BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 – Programa Operacional Regional do Norte, PEst-OE/EQB/LA0004/2011 and iNOVA4Health – UID/Multi/04462/2013, a program financially supported by FCT/Ministério da Educação e Ciência, through national funds and co-funded by FEDER under the PT2020 Partnership Agreement. The authors also acknowledge the European Commission: BIOCAPS (316265, FP7/REGPOT-2012-2013.1). This work was also supported by the “CARINA” project for the safeness, sustainability and competitiveness of agro-food productions of Campania Region. The support of EU Cost Action FA1001 is gratefully acknowledged. The authors also acknowledge Stepan (The Netherlands) for providing the Neobee 1053 oil, and Rui Fernandes from IBMC, University of Porto for assistance in taking the TEM pictures.info:eu-repo/semantics/publishedVersio
PRINCÍPIOS DA MATEMÁTICA ESCOLAR RECONTEXTUALIZADOS DE DISCIPLINAS ESPECÍFICAS
No full textJaqueline de Souza Pereira Grilo[1]Jonei Cerqueira Barbosa[2]Ana Virgínia de Almeida Luna[3][1] Mestre em Educação pela UFBA. Profª Assistente da UFRB. [email protected][2] Docente do Programa de Pós-graduação em Educação da UFBA e do PPGEFHC/UFBA-UEFS. [email protected].[3] Docente do Programa de Pós-graduação em Educação da UEFS. [email protected]
Campanha de escavação e consolidação da mamoa 1 da Cerqueira (Serra do Arestal - Sever do Vouga)
No full text[Excerto] Introdução: A mamoa/dólmen 1 de Cerqueira ou Casa da Moura, nomes pelo qual o imóvel é conhecido, insere-se numa necrópole que conta actualmente com oito monumentos dos onze detectados nos anos cinquenta1. Cinco destes monumentos apresentam características megalíticas evidentes, sendo os restantes poucos perceptíveis na paisagem, motivo pelo qual evitámos nomear esta necrópole de megalítica. A área ocupada pela necrópole não devia ultrapassar um hectare embora tenhamos que admitir a existência de outros monumentos, que por serem pouco perceptíveis nos tenham escapado nos trabalhos de prospecção que realizámos. Apesar da mamoa 1 da Cerqueira ter sido escavada em 19562, achámos necessário intervencionar de novo nesse monumento devido ao estado adiantado de erosão que nele se fazia sentir e pela necessidade de estendermos os estudos à área circundante da câmara e corredor. Do mesmo modo, pretendemos consolidar e restaurar este monumento, que é hoje de valor concelhio. (...)info:eu-repo/semantics/publishedVersio
Representações da sexualidade na obra de Ana Saldanha
No full textMestrado em Línguas, Literaturas e CulturasA presente dissertação analisa as diferentes representações da sexualidade na obra literária para jovens de Ana Saldanha. A leitura analítica incide sobre as narrativas da autora que abordam a temática da sexualidade juvenil e as suas problemáticas, com o objetivo de estudar a forma como as mesmas contribuem para a construção da identidade pessoal e sexual do potencial recetor juvenil. Desta forma, pretende-se detetar os conflitos psicológicos dos protagonistas, os seus valores e problemas, assim como os novos valores, problemas e preocupações sociais que emergem das narrativas; compreender de que forma a autora exprime a sua capacidade em observar o quotidiano e as relações humanas; identificar os pilares em que se apoia o universo social em estudo (família, escola, grupo de amigos); enquadrar as personagens numa sociedade pós-industrial e de informação que exige ao adolescente uma superação de desafios e de novos problemas.This dissertation analyzes the different representations of sexuality in Ana Saldanha’s literary work for youth. The analytical reading focuses on the author's narratives that address the issue of teenage sexuality and its problems, aiming to study their contribution in the construction of personal and sexual identity of the potential juvenile receiver. Thus, it is intended to detect the psychological conflicts of the protagonists, their values and problems, as well as the new values, social concerns and problems that emerge from the narratives; understand how the author expresses her ability to observe daily life and human relations; identify the pillars on which relies the social universe under study (family, school, friends); framing the characters in a post-industrial and information society which requires the teenager to relentlessly surmount challenges and new problems
Euseius alatus De Leon 1966
No full textEuseius alatus De Leon Material examined: Jaguanum Island: 3 females on unidentified plant, 19-IV-2007.Published as part of Cavalcante, Ana Cristina Cerqueira, Demite, Peterson Rodrigo, Lofego, Antônio Carlos & Hernandes, Fabio Akashi, 2021, Phytoseiidae (Acari: Mesostigmata) from the Atlantic Forest in Rio de Janeiro, Brazil, with complementary description of Amblyseius impeltatus Denmark & Muma, pp. 1-7 in Papéis Avulsos de Zoologia 61 on page 5, DOI: 10.11606/1807-0205/2021.61.98, http://zenodo.org/record/717699
Phytoseiidae (Acari: Mesostigmata) from the Atlantic Forest in Rio de Janeiro, Brazil, with complementary description of Amblyseius impeltatus Denmark & Muma
No full textCavalcante, Ana Cristina Cerqueira, Demite, Peterson Rodrigo, Lofego, Antônio Carlos, Hernandes, Fabio Akashi (2021): Phytoseiidae (Acari: Mesostigmata) from the Atlantic Forest in Rio de Janeiro, Brazil, with complementary description of Amblyseius impeltatus Denmark & Muma. Papéis Avulsos de Zoologia 61: 1-7, DOI: 10.11606/1807-0205/2021.61.98, URL: http://dx.doi.org/10.11606/1807-0205/2021.61.9
Proprioseiopsis dominigos
No full textProprioseiopsis dominigos (El-Banhawy) Material examined: Paraty: 1 female on Cupania sp., 27-VII-2008.Published as part of Cavalcante, Ana Cristina Cerqueira, Demite, Peterson Rodrigo, Lofego, Antônio Carlos & Hernandes, Fabio Akashi, 2021, Phytoseiidae (Acari: Mesostigmata) from the Atlantic Forest in Rio de Janeiro, Brazil, with complementary description of Amblyseius impeltatus Denmark & Muma, pp. 1-7 in Papéis Avulsos de Zoologia 61 on page 5, DOI: 10.11606/1807-0205/2021.61.98, http://zenodo.org/record/717699
Figure 8 in Phytoseiidae (Acari: Mesostigmata) from the Atlantic Forest in Rio de Janeiro, Brazil, with complementary description of Amblyseius impeltatus Denmark & Muma
No full textFigure 8. Amblyseius impeltatus Denmark & Muma. Holotype spermatheca (Photo: Samuel Bolton).Published as part of Cavalcante, Ana Cristina Cerqueira, Demite, Peterson Rodrigo, Lofego, Antônio Carlos & Hernandes, Fabio Akashi, 2021, Phytoseiidae (Acari: Mesostigmata) from the Atlantic Forest in Rio de Janeiro, Brazil, with complementary description of Amblyseius impeltatus Denmark & Muma, pp. 1-7 in Papéis Avulsos de Zoologia 61 on page 5, DOI: 10.11606/1807-0205/2021.61.98, http://zenodo.org/record/717699
Phytoseius marumbus El-Banhawy 1984
No full textPhytoseius marumbus El-Banhawy Material examined: Jaguanum Island: 1 female on unidentified plant, 19-IV-2007.Published as part of Cavalcante, Ana Cristina Cerqueira, Demite, Peterson Rodrigo, Lofego, Antônio Carlos & Hernandes, Fabio Akashi, 2021, Phytoseiidae (Acari: Mesostigmata) from the Atlantic Forest in Rio de Janeiro, Brazil, with complementary description of Amblyseius impeltatus Denmark & Muma, pp. 1-7 in Papéis Avulsos de Zoologia 61 on page 5, DOI: 10.11606/1807-0205/2021.61.98, http://zenodo.org/record/717699
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