170,174 research outputs found
Transzcelluláris bikarbonát szekréció és intracelluláris pH reguláció polarizált epitheliás sejtekben = Transcellular bicarbonate secretion and intracellular pH regulation on polarized epithelial cells
No full textKutatásunk célja polarizált epitheliális sejtek elektrolit és folyadékszekréciójának molekuláris szintű vizsgálata volt. Jól differenciált hasnyálmirigy duktusz Capan-1 és HPAF, valamint nyálmirigy acinus ParC10 és SMIE sejtvonalakat vizsgáltunk molekuláris és funkcionális eszközrendszer alkalmazásával. Polarizált sejttenyészeteket hoztunk létre permeábilis membránon, és vizsgáltuk génexpressziós mintázatukat RT-PCR-rel, anionszekréciójukat Ussing kamrás módszerrel és intracelluláris pH-szabályozásukat mikrofluorometriás pH-mérés segítségével. A vízszekrécióért felelős mechanizmusokat szöveti immunhisztokémiai módszerekkel tanulmányoztuk. Kimutattuk Capan-1, HPAF és Par-C10 sejtekben az NBC, NHE1, NKCC1, CFTR mRNS-ek expresszióját. Igazoltuk, hogy patkány és emberi hasnyálmirigyben koexpresszálódik az AQP1 és AQP5 csatorna az intraloburális duktuszok apikális felszínén. Ussing-kamrás kísérleteink során bizonyítottuk, hogy az HPAF és Par-C10 sejtvonalak képesek vektoriális anion szekrécióra, és ez gátolható az NKCC gátlásával. Sikerült igazolnunk, hogy a Capan-1, HPAF és Par-C10 sejtvonalakban a HCO3- utánpótlását a bazolaterálisan elhelyezkedő NBC és NHE együttesen szolgáltatják. Eredményeink szerint ParC10 sejtek bazolaterális és apikális felszínén is találhatóak anion-kicserélők. Az általunk jellemzett modellrendszerek jól alkalmazhatók a továbbiakban a humán külső elválasztású mirigyek folyadék- és elektrolit szekréció rendellenességeinek modellezésére. | We studied the molecular mechanisms of the electrolyte and fluid secretion of polarized epithelial cells. We have used well differentiated Capan-1 and HPAF pancreas ductal and Par-C10 and SMIE salivary acinar cell lines for molecular and functional experiments. The cells were seeded on permeable support and formed polarized cultures. RT-PCR tests were taken to examine the gene expression of the cells. We have investigated the anion secretion with Ussing chamber, and intracellular pH with microfluorometric measurements. The mechanisms responsible for transcellular water secretion were studied by tissue immunohystochemistry. We have shown the Capan-1, HPAF and Par-C10 cells express the mRNA of NBC, NHE1, NKCC1 and CFTR. We have demonstrated that AQP1 and AQP5 aquaporin water channels colocalize on the apical membrane of rat and human pancreas intercalated ductal cells. With Ussing-chamber experiments it was detected that the HPAF and Par-C10 cells are capable of vectorial anion secretion and that this secretion can be blocked by the inhibition of NKCC. We could demonstrate that the HCO3- influx happens through the basolateral NHE and NBC transporters in Capan-1, HPAF and Par-C10 cells. Our results show that anion exchangers work in both the basolateral and apical membranes of Par-C10 cells. The characterized polarized cell systems are suitable model systems for examining the fluid- and electrolyte secretory transport function disorders of human exocrine glands
Enrichment analysis of Capan-1 Hi-C data by g:Profiler.
No full textEnrichment analysis of Capan-1 Hi-C data by g:Profiler.</p
Study of the enhanced anticancer efficacy of gambogic acid on Capan-1 pancreatic cancer cells when mediated via magnetic Fe3O4 nanoparticles
No full textCailian Wang1,*, Haijun Zhang1,*, Baoan Chen2, Haitao Yin1, Wenwen Wang11Department of Oncology, Zhongda Hospital, Medical School, Southeast University, Nanjing, People&#39;s Republic of China; 2Department of Hematology, Zhongda Hospital, Medical School, Southeast University, Nanjing, People&#39;s Republic of China *These authors contributed equally to this workBackground: Gambogic acid (GA), a potent anticancer agent, is limited in clinical administration due to its poor water solubility. The aim of this study was to explore a drug delivery system based on magnetic Fe3O4 nanoparticles (MNP- Fe3O4) conjugated with GA to increase water solubility of the drug and enhance its chemotherapeutic efficiency for pancreatic cancer.Methods: GA was conjugated with the MNP- Fe3O4 colloidal suspension by mechanical absorption polymerization to construct GA-loaded MNP- Fe3O4, which acted as a drug delivery system.Results: Combination therapy with GA and MNP- Fe3O4 induced remarkable improvement in anticancer activity, which was demonstrated by optical microscopic observations, MTT assay, and nuclear DAPI staining. Furthermore, the possible signaling pathway was explored by Western blot. In Capan-1 pancreatic cancer cells, our observations demonstrated that this strategy could enhance potential anticancer efficiency by inducing apoptosis. The mechanisms of the synergistic effect may be due to reducing protein expression of Bcl-2 and enhancing that of Bax, caspase 9, and caspase 3.Conclusion: These findings demonstrate that a combination of GA and MNPs- Fe3O4 represents a promising approach to the treatment of pancreatic cancer.Keywords: gambogic acid, pancreatic cancer, magnetic nanoparticles, drug delivery system, apoptosi
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Effect of Ani9 on endogenous CaCCs in PC3, Capan-1 and NHNE cells.
No full textA) Immunoblot of ANO1 protein in FRT, FRT-ANO1, PC3, Capan-1 and NHNE cells. Blots shown are representative of experiments performed three times. B-C) Effect of Ani9 on CaCCs activity was measured in PC3 and Capan-1 cells expressing a halide sensor YFP. The indicated concentrations of Ani9 were applied 20 min prior to CaCCs activation by 100 μM ATP. D) Summary of dose response (mean ± S.E., n = 6). E) Effect of Ani9 on CaCCs activity in IL-4 treated (10 ng/mL; 48h) NHNE cells. CaCCs currents were induced by 100 μM ATP and then the indicated concentrations of Ani9 were applied. The remaining CaCCs currents were abolished by 100 μM tannic acid (TA). (right) Summary of dose response (mean ± S.E., n = 4).</p
Mitomycin C in highly myopic eyes - Author reply
No full textOphthalmology. 2005 Feb;112(2):208-18; discussion 219.
Mitomycin C modulation of corneal wound healing after photorefractive keratectomy in highly myopic eyes.
Gambato C, Ghirlando A, Moretto E, Busato F, Midena E.
SourceRefractive Surgery Service and Antimetabolite Therapy Research Unit, Department of Ophthalmology, University of Padova, Padova, Italy.
Abstract
PURPOSE: To evaluate the role of topical mitomycin C in corneal wound healing (CWH) after photorefractive keratectomy (PRK) in highly myopic eyes.
DESIGN: Prospective, double-masked, randomized clinical trial.
PARTICIPANTS: Seventy-two eyes of 36 patients affected by high (>7 diopters) myopia.
METHODS: In each patient, one eye was randomly assigned to PRK with intraoperative topical 0.02% mitomycin C application, and the fellow eye was treated with a placebo. Postoperatively, mitomycin C-treated eyes received artificial tears (3 times daily, tapered in 3 months), whereas the fellow eye was treated with fluorometholone sodium 2% and artificial tears (3 times daily, tapered in 3 months).
MAIN OUTCOME MEASURES: Uncorrected visual acuity (UCVA) and best-corrected visual acuity (BCVA), contrast sensitivity, manifest refraction, and biomicroscopy. Contrast sensitivity was determined using the Pelli-Robson chart. Corneal confocal microscopy documented CWH.
RESULTS: Mean follow-up was 18 months (range, 12-36). No side effects or toxic effects were documented. At 12-month follow-up examination, UCVAs (logarithm of the minimum angle of resolution) were 0.4+/-0.48 and 0.5+/-0.53 (P = .03) in mitomycin C-treated eyes and corticosteroid-treated eyes, respectively. At 1 year, corneal haze developed in 20% of corticosteroid-treated eyes, versus 0% of mitomycin C-treated eyes. At 12, 24, and 36 months, corneal confocal microscopy showed activated keratocytes and extracellular matrix significantly more evident in untreated eyes (Ps = 0.004, 0.024, and 0.046, respectively).
CONCLUSION: Topical intraoperative application of 0.02% mitomycin C can reduce haze formation in highly myopic eyes undergoing PRK.
Comment in
Ophthalmology. 2006 Feb;113(2):357; author reply 357-8
Dispelling the Myths Behind First-author Citation Counts
No full textWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
LDH-releasing assays were performed to confirm the appropriate conditions for Tmab-mediated ADCC against Capan-1, a HER2-high-expressing human pancreatic cancer cell line.
No full textA: The contribution of Tmab to ADCC against Capan-1 was confirmed. The effector/target (E/T) ratio was set at 10:1, and 10 μg/ml of Tmab and isotype IgG were used. B: To confirm the appropriate concentration of Tmab, ADCC with the indicated dose of Tmab was measured. The E/T ratio was set at 40:1. C: To determine the necessity for the preadministration of Tmab to target cells, Capan-1 was incubated with 10 μg/ml of Tmab for the indicated times. Cells were harvested and ADCC was evaluated. The E/T ratio was set at 40:1.</p
COSMC KO renders Capan-1 and T47D cells more susceptible to ADCC.
No full text<p>A)–C) WT and COSMC KO target cells were labeled with chromium and co-cultured with PBMC effector cells in an E/T ratio of 50∶1 (black) and 25∶1 (white) either with or without Ab (Erbitux®). After 4 hrs the chromium release into the media was measured and the % specific killing was calculated. The bars show mean +/− SD from quadruplicates. Student's two-tailed unpaired t test was applied to test for significance, stars indicate level of significance. Experiments performed with two to six different PBMC donors. Data from one representative donor is shown. Experiments where cells were treated with +/− neuraminidase to remove sialic acid before ADCC is included in B) and COSMC KO cells transiently rescued with COSMC pcDNA3 transfection as well as mock transfected control are included in C). D) Capan-1 and T47D were treated with hydrogen peroxide for 20 hrs (0–0.2 mM range for Capan-1 and 0–0.5 mM range for T47D) prior to apoptosis staining using Annexin V and PI. Annexin V positive cells are considered early apoptotic whereas double positive cells are considered late apoptotic. The % positive is defined by the percent of cells above the MFI of an unstained background control in a flow cytometric analysis. One representative data set is shown out of two to three individual experiments performed.</p
Characterization of trypsinogens 1 and 2 in two human pancreatic adenocarcinoma cell lines; CFPAC-1 and CAPAN-1
No full textAbstractProteins with trypsin-like immunoreactivity (first detected by a specific immunoenzymatic assay) were isolated from CAPAN-1 and CFPAC-1 cell culture-conditioned media by chromatography on an immunoadsorbent prepared with a polyclonal antibody directed against trypsin 1. The adsorbed proteins were devoid of free trypsin activity but trypsin activity was present after enterokinase activation demonstrating that the immunoreactive trypsin present in cell supernatants corresponds to trypsinogens. When characterised by Western blotting using a monoclonal antibody directed against human trypsin 1 two protein bands corresponding to trypsinogen 1 (23 kDa) and trypsinogen 2 (25 kDa) gave a positive reaction. These results demonstrate the presence of trypsinogens 1 and 2 in CAPAN-1 and CFPAC-1 cells and in their culture-conditioned media
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