100,929 research outputs found
IDENTIFICATION OF LYCHNIS RINGSPOT VIRUS IN MENTHA PIPERITA IN ITALY BY NEXT GENERATION SEQUENCING (NGS) TECHNOLOGY. G. Parrella, M.G. Bellardi, I. Bornard, C. Faure, S. Theil, A. Marais, T. Candresse
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FAIR CT 97-3889: Health certification of rosaceous species based on disease-indexing of in vitro plants: Validation of diagnostics and diagnostic strategies
No full textAim of the project FAIR CT 97-3889 was to develop and assess broad spectrum and specific assays for detection of filamentous, bacilliform and nematode-transmitted viruses and phytoplasmas to be used in tissue culture laboratories involved in the production of certified elite propagation material. The methodology to be used for broad-spectrum and/or specific detection tests (RT-PCR, PCR-ELISA, NASBA) is based on the amplification of the pathogen genome. Elimination procedures, such as meristem culture, heat therapy, and combinations thereof, were compared to evaluate their efficiency to eliminate recalcitrant pathogens. The release of fruit plant cultivars to fruit plant growers takes several years until all known as well as diseases of unknown etiology related with a plant species are checked by indexing methods currently in use ("base line" tests are carried out in the field with woody indicators). The combination of disease elimination and disease-indexing on in vitro plants, using reliable laboratory diagnostics, would considerably reduce the efforts and contribute to savings of time, money and labour and constitutes a considerable step forward to avoid disease spread and to allow safe commercial transactions of propagating plant material
Investigation on occurrence of Tricho- Fovea- and Capilloviruses in ancient fruit trees cultivars in Campania
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Indexing and variability of Tricho-, Fovea- and Capilloviruses in ancient fruit tree cultivars in Campania
No full textLetter, [Author unclear] to Paulina T. Merritt
No full textHandwritten letter to Paulina Merritt from an unknown author, October 1, 1876.
Recent advances and prospects in<i>Prunus</i>virology
No full text[EN] The stone fruit genus Prunus, within the family Rosaceae, comprises more than
230 species, some of which have great importance or value as ornamental
or fruit crops. Prunus are affected by numerous viruses and viroids linked
to the vegetative propagation practices in many of the cultivated species.
To date, 44 viruses and three viroids have been described in the 9 main
cultivated Prunus species. Seven of these viruses and one viroid have been
identified in Prunus hosts within the last 5 years. This work addresses recent
advances and prospects in the study of viruses and viroids affecting Prunus
species, mostly concerning the detection and characterisation of the agents
involved, pathogenesis analysis and the search for new control tools. New
sequencing technologies are quickly reshaping the way we can identify and
characterise new plant viruses and isolates. Specific efforts aimed at virus
identification or data mining of high-throughput sequencing data generated for
plant genomics-oriented purposes can efficiently reveal the presence of known
or novel viruses. These technologies have also been used to gain a deeper
knowledge of the pathogenesis mechanisms at the gene and miRNA expression
level that underlie the interactions between Prunus spp. and their main viruses
and viroids. New biotechnological control tools include the transfer of resistance
by grafting, the use of new sources of resistance and the development of gene
silencing strategies using genetic transformation. In addition, the application of
next generation sequencing and genome editing techniques will contribute to
improving our knowledge of virus¿host interactions and the mechanisms of
resistance. This should be of great interest in the search to obtain new Prunus
cultivars capable of dealing both with known viruses and viroids and with those
that are yet to be discovered in the uncertain scenario of climate change.The authors offer grateful thanks to Spanish Ministry of Economy and Competitiveness for the Ramon y Cajal contract (RYC-2013-12563) of Dr. Manuel Rubio. This study has been supported by the projects 'Molecular and Genetic bases of multiple resistance to Plum pox virus (PPV) and Apple chlorotic leaf spot virus (ACLSV) in apricot' (AGL2015-68021-R) from the Spanish Ministry of Economy and Competiveness and 'Breeding stone fruit species assisted by molecular tools' from the Seneca Foundation of the Region of Murcia (19879/GERM/15).Rubio, M.; Martinez-Gomez, P.; Marais, A.; Sanchez Navarro, JA.; Pallás Benet, V.; Candresse, T. (2017). Recent advances and prospects in Prunus virology. Annals of Applied Biology. 171(2):125-138. https://doi.org/10.1111/aab.12371S1251381712Angelova L. Stoev A. Borisova E. Avramov L. 2017 Proceedings of SPIE 10226, 19th International Conference and School on Quantum Electronics: Laser Physics and Applications, 1022614 https://doi.org/10.1117/12.2261807Babu, M., Griffiths, J. S., Huang, T.-S., & Wang, A. (2008). Altered gene expression changes in Arabidopsis leaf tissues and protoplasts in response to Plum pox virus infection. BMC Genomics, 9(1), 325. doi:10.1186/1471-2164-9-325Bag, S., Al Rwahnih, M., Li, A., Gonzalez, A., Rowhani, A., Uyemoto, J. K., & Sudarshana, M. R. (2015). Detection of a New Luteovirus in Imported Nectarine Trees: A Case Study to Propose Adoption of Metagenomics in Post-Entry Quarantine. Phytopathology®, 105(6), 840-846. doi:10.1094/phyto-09-14-0262-rBarba, M., Czosnek, H., & Hadidi, A. (2014). Historical Perspective, Development and Applications of Next-Generation Sequencing in Plant Virology. Viruses, 6(1), 106-136. doi:10.3390/v6010106Barba, M., Ilardi, V., & Pasquini, G. (2015). Control of Pome and Stone Fruit Virus Diseases. Control of Plant Virus Diseases - Vegetatively-Propagated Crops, 47-83. doi:10.1016/bs.aivir.2014.11.001Candresse T. Martínez-Gómez P. Rubio M. 2015 XXIII International Conference on Virus and Other Graft Transmissible Diseases of Fruit CropsChirkov, S., Ivanov, P., & Sheveleva, A. (2013). Detection and partial molecular characterization of atypical plum pox virus isolates from naturally infected sour cherry. Archives of Virology, 158(6), 1383-1387. doi:10.1007/s00705-013-1630-xChirkov, S., Ivanov, P., Sheveleva, A., Zakubanskiy, A., & Osipov, G. (2017). New highly divergent Plum pox virus isolates infecting sour cherry in Russia. Virology, 502, 56-62. doi:10.1016/j.virol.2016.12.016Clemente-Moreno, M. J., Hernández, J. A., & Diaz-Vivancos, P. (2014). Sharka: how do plants respond to Plum pox virus infection? Journal of Experimental Botany, 66(1), 25-35. doi:10.1093/jxb/eru428García, J. A., Glasa, M., Cambra, M., & Candresse, T. (2014). 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A remarkable synergistic effect at the transcriptomic level in peach fruits doubly infected by prunus necrotic ringspot virus and peach latent mosaic viroid. Virology Journal, 10(1). doi:10.1186/1743-422x-10-164Ilardi, V., & Tavazza, M. (2015). Biotechnological strategies and tools for Plum pox virus resistance: trans-, intra-, cis-genesis, and beyond. Frontiers in Plant Science, 6. doi:10.3389/fpls.2015.00379James, D., Varga, A., & Lye, D. (2014). Analysis of the complete genome of a virus associated with twisted leaf disease of cherry reveals evidence of a close relationship to unassigned viruses in the family Betaflexiviridae. Archives of Virology, 159(9), 2463-2468. doi:10.1007/s00705-014-2075-6Jung, S., Jiwan, D., Cho, I., Lee, T., Abbott, A., Sosinski, B., & Main, D. (2009). Synteny of Prunus and other model plant species. BMC Genomics, 10(1), 76. doi:10.1186/1471-2164-10-76Lenz, O., Přibylová, J., Fránová, J., Koloniuk, I., & Špak, J. (2016). Identification and characterization of a new member of the genus Luteovirus from cherry. Archives of Virology, 162(2), 587-590. doi:10.1007/s00705-016-3125-zLin, L., Li, R., Bateman, M., Mock, R., & Kinard, G. (2013). Development of a multiplex TaqMan real-time RT-PCR assay for simultaneous detection of Asian prunus viruses, plum bark necrosis stem pitting associated virus, and peach latent mosaic viroid. European Journal of Plant Pathology, 137(4), 797-804. doi:10.1007/s10658-013-0289-1Marais, A., Svanella-Dumas, L., Barone, M., Gentit, P., Faure, C., Charlot, G., … Candresse, T. (2011). Development of a polyvalent RT-PCR detection assay covering the genetic diversity of Cherry capillovirus A. Plant Pathology, 61(1), 195-204. doi:10.1111/j.1365-3059.2011.02488.xMarais, A., Faure, C., Couture, C., Bergey, B., Gentit, P., & Candresse, T. (2014). Characterization by Deep Sequencing of Divergent Plum bark necrosis stem pitting-associated virus (PBNSPaV) Isolates and Development of a Broad-Spectrum PBNSPaV Detection Assay. Phytopathology®, 104(6), 660-666. doi:10.1094/phyto-08-13-0229-rMarais, A., Faure, C., Mustafayev, E., & Candresse, T. (2015). Characterization of New Isolates of Apricot vein clearing-associated virus and of a New Prunus-Infecting Virus: Evidence for Recombination as a Driving Force in Betaflexiviridae Evolution. PLOS ONE, 10(6), e0129469. doi:10.1371/journal.pone.0129469Marais, A., Faure, C., Mustafayev, E., Barone, M., Alioto, D., & Candresse, T. (2015). Characterization by Deep Sequencing of Prunus virus T, a Novel Tepovirus Infecting Prunus Species. Phytopathology®, 105(1), 135-140. doi:10.1094/phyto-04-14-0125-rMarais, A., Faure, C., Theil, S., Svanella-Dumas, L., Brans, Y., Maurice, I., … Candresse, T. (2016). First Report of Little cherry virus 1 on Plum in France. 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A Framework for the Evaluation of Biosecurity, Commercial, Regulatory, and Scientific Impacts of Plant Viruses and Viroids Identified by NGS Technologies. Frontiers in Microbiology, 8. doi:10.3389/fmicb.2017.00045Matic, S., Minafra, A., Sánchez-Navarro, J. A., Pallás, V., Myrta, A., & Martelli, G. P. (2009). ‘Kwanzan Stunting’ syndrome: Detection and molecular characterization of an Italian isolate of Little cherry virus 1. Virus Research, 143(1), 61-67. doi:10.1016/j.virusres.2009.03.005Navarro, B., Gisel, A., Rodio, M. E., Delgado, S., Flores, R., & Di Serio, F. (2012). Small RNAs containing the pathogenic determinant of a chloroplast-replicating viroid guide the degradation of a host mRNA as predicted by RNA silencing. The Plant Journal, 70(6), 991-1003. doi:10.1111/j.1365-313x.2012.04940.xPallas, V., Aparicio, F., Herranz, M. C., Amari, K., Sanchez-Pina, M. A., Myrta, A., & Sanchez-Navarro, J. A. (2012). Ilarviruses of Prunus spp.: A Continued Concern for Fruit Trees. 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First report of Kalanchoe mosaic virus and kalanchoe latent virus infecting ghost plant (Graptopetalum paraguayense) in Italy
No full textFirst report of [i]Kalanchoe mosaic virus[/i] and [i]Kalanchoe latent virus[/i] infecting Ghost plant ([i]Graptopetalum paraguayense W.[/i]) in Ital
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