112,104 research outputs found
author-bios-SRD-19-0063.R1 – Supplemental material for The Network Structure of Police Misconduct
No full textSupplemental material, author-bios-SRD-19-0063.R1 for The Network Structure of Police Misconduct by George Wood, Daria Roithmayr and Andrew V. Papachristos in Socius</p
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Structure of the Ki-Ras Gene of the Human-Lung Carcinoma Cell-Line Calu-1
No full textThe homologue of the viral Kirsten ras (v-Ki-ras) gene found in the human lung carcinoma cell line, Calu-1, has an intron-exon structure similar to that of the human homologue of the viral Harvey ras (v-Ha-ras) gene. A second, potential fourth coding exon is present in the human Ki-ras gene and similar sequences are found in the Kirsten murine sarcoma virus. Cysteine is encoded at the twelfth amino acid position, suggesting that the Calu-1 Ki-ras gene has undergone a mutational activation at the same position as the human Ha-ras gene of the bladder carcinoma cell line, T24. A comparison of their predicted amino acid sequences suggests that ras proteins have a 'constant' region and a 'variable' region. Here we propose a common modular structure for ras gene products in which the variable region forms a physiologically important combining site
Variations on the Author
No full text“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
CALU Recuperar para Resignificar
No full textTodo proyecto nace de una chispa, una pregunta o una conexión inesperada. En mi caso, este viaje comenzó con la búsqueda de un equilibrio entre mi pasión por el diseño de atmósferas, mi interés por los materiales y el deseo de explorar el impacto de mi trabajo como futura diseñadora. La idea de Calu no surgió de manera inmediata, sino que fue el resultado de una serie de encuentros, reflexiones y decisiones que me llevaron a cuestionar el concepto de residuo y a imaginar nuevas formas de creación a partir de aquello que otros consideran desecho.
Calu es un proyecto que explora el potencial de los biomateriales para transformar residuos en nuevos sistemas de valor. En este caso, se trata de un sistema de unión que utiliza retazos de madera, organizados intencionalmente, ensamblados mediante un biomaterial a base de cáscara de huevo. Este sistema permite recuperar tanto el valor estético como funcional de materiales que de otra manera serían descartados.
Recuperar es un acto de resignificar, una forma de mirar aquello que ha sido descartado y encontrar en ello un nuevo propósito. Es en este espacio de transformación donde los residuos se convierten en una declaración: lo que parece ser un desecho puede, en realidad, ser valioso. Uno de los resultados tangibles de este sistema es un taburete, que no solo refleja la funcionalidad del biomaterial y el sistema de unión, sino también la posibilidad de generar diseños sostenibles que cuestionan y revalorizan nuestra relación con los desechos.Pregrad
Triamterene does not reduce Calu-3 viability.
No full textCellTiter Blue non-destructive assay was used to evaluate changes in cell viability of CALU-3 cells following 48h treatment with Triamterene following a replenishment protocol where drug and media were replaced at 24h. Data is presented as mean of percentage of DMSO treated control ± standard error (Mean ± SEM). Toxicity is defined as >20% loss of viability compared to mock control. Asterisks indicate significant differences from the DMSO treated control by ordinary one-way analysis of variance with Dunnett’s multiple-comparison test (P (PDF)</p
Clopidogrel pretreatment does not reduce Calu-3 viability.
No full textA CellTiter Blue assay was used to evaluate Calu-3 cell viability. Following 48h treatment with Clopidogrel where the drug and media were replaced at 24h the mean percent of DMSO-treated control ± standard error was determined. Toxicity was defined as ≥20% loss of viability compared to the mock control. Asterisks indicate significant differences from the DMSO treated control by one-way analysis of variance with Dunnett’s multiple-comparison test (P (PDF)</p
Preliminary Studies on Validation of Calu-3 Cell Line as a Model for Screening Respiratory Mucosa Irritation and Toxicity
No full textThere is need to develop reproducible methods and experimental models for screening mucosal irritation and toxicity for drugs and pharmaceutical excipients. The aim of this study was to validate Calu-3 cell line as a model for screening respiratory irritation and toxicity of drugs and excipients. Eighteen test compounds were selected according to their irritation potential and European Centre for the Validation of Alternative Methods (ECVAM) guidelines. Cell toxicity and irritation was determined using MTT assay. Data analysis and interpretation were done using modified ECVAM approach; where replicate values met acceptance criteria if percent relative standard deviation (RSD) of the raw data is <18%. Compounds with mean relative viability values of 50% and below were classified as irritant (I); those above 50% were non-irritant (NI). At low concentration (0.2% w/v) and 1 h incubation, the Calu-3 cell culture model accurately predicted the toxicity of most test compounds. The specificity of our proposed model (percentage of in vivo non-irritants correctly predicted), concordance (percentage of compounds correctly predicted) and sensitivity (percentage of in vivo irritants correctly predicted) at 0.2% w/v and 60 min exposure were 100%, 72%, and 44%, respectively. In conclusion, the Calu-3 cell line in conjunction with MTT assay appears to be a potentially useful tool for screening drugs and excipients for respiratory mucosa irritation and toxicity. However, as the data reported in this study were solely based on MTT assay, additional studies are needed using other toxicity-/irritation-indicating methods to confirm the observed trend
Positions of transcription start sites in single and double promoters in the Calu-1 and A375 cell lines.
No full text<p><b>A:</b> Schematic representation of transcription start sites (TSSs) in different promoters under study. Upper and lower arrows mark TSSs in the Calu-1 and A375 cells, respectively. PhSurv, human survivin gene promoter; PhTERT, human telomerase reverse transcriptase promoter. Numbers at the broken arrows represent the number of clones containing the corresponding transcription start site. At least 12 clones were analyzed for each construct. The promoter schemes are out of scale. Actually, all PhTERTs and all the PhSurv promoters have the length of about 240 and 1500 bp, respectively. <b>B:</b> Positions of TSSs in single and double promoters in the Calu-1 and A375 cell lines. 60 bp of 3′ promoter regions are shown. TSSs identified in this work for the Calu-1 cell line are shown in bold, and for A375 – underlined; TSSs identified earlier <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0046474#pone.0046474-Li1" target="_blank">[12]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0046474#pone.0046474-Takakura1" target="_blank">[17]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0046474#pone.0046474-Horikawa1" target="_blank">[23]</a> are marked by rectangles.</p
Využití sociálních médií v B2B prodeji
No full textTato diplomová práce se zabývá tím, jak mohou B2B obchodníci využívat sociální média v prodeji. Na základě systematické rešerše literatury, autor zjistil, že akademici, zkoumající danou problematiku, navrhují další výzkum, a to: v kterých konkrétních krocích se dají využít sociální média v prodeji (Salo, 2017). Autor se na základě toho rozhodl zjistit, jaké sociální sítě, různé technologie a pluginy se dají využít v B2B prodeji - tzv. social sellingu. Social selling se v této práci týká primárně procesu akvizice a okrajově péčí o stávající zákazníky. Autor si vybral kvalitativní průzkum pomocí 10 hloubkových polo-strukturovaných rozhovorů, aby odhalil jak, která sociální média to jsou, tak i motivaci prodejců, proč tato média používat/nepoužívat. Aby autor dodržel správnost vyhodnocení výsledků, data byla analyzována pomocí Tématické analýzy, která v této studii vykrystalizovala 2 hlavní strategické přístupy v social sellingu. Tyto přístupy (tzv. Push a Pull strategie) obsahují praktické příklady a konkrétní aktivity, které mohou prodejci využívat v každodenní praxi. Tyto výsledky jsou prezentovány s důrazem na praktičnost a jednoduchost implementace. Tvoří proto hlavní přínos autorovo výzkumu. V poslední části autor zmiňuje výzvy a manažerská doporučení, které mohou obchodníci využít v každodenním pracovním životě.This diploma thesis focuses on social media usage in B2B sales. Based on the systematic literature review conducted by the author, he has found out that recent researchers (Salo, 2017) suggest further research in the area of how and in which sales phase should various social networking sites, technologies and plugins used. To further fill this research gap, author decided to identify these social media and their usage among B2B salespeople in the so-called social selling process. The social selling process in this thesis applies mainly to acquiring new prospects and tangentially to taking care of existing clients (follow-up step). Author has chosen a qualitative research method via conducting 10 in-depth semi-structured interviews to reveal these instruments as well as motivation of a sales person on why to use social media in the selling process. The collected data was analyzed using Thematic analysis to ensure the right procedure and to identify main themes which crystalized into 2 main strategic approaches in social selling. These approaches (Push and Pull) include practical examples of concrete activities which sales people can use in their daily jobs and are presented with focus on practicality and ease of implementation. These also form the main contribution of author`s research. In the last part, author mentions challenges in social selling and recommended managerial implications for salesforce
- …
