1,720,959 research outputs found
Cytotoxic Effect of Zinc Oxide and Selenium Nanoparticles Synthesized From Orobanche Picridis on Lung Cancer as Well as Their Antimicrobial and Antioxidant Properties
No full textErcan, Leyla/0000-0002-6570-8128Orobanche picridis represents a fascinating example of an obligate parasitic plant due to its lack of chlorophyll. It is of significant interest to botanists, ecologists, and biologists because of its complex parasitic lifestyle and specific host preferences. The purpose of this work was to generate zinc oxide (OrZnO) nanoparticles (NPs) and selenium (OrSe) nanoparticles from O. picridis and examine their characterization as well as their antibacterial, anticarcinogenic, and antioxidant characteristics. To this purpose, the produced NPs were characterized utilizing a scanning electron microscope, energy-dispersive X-ray spectroscopy, X-ray diffraction, and Fourier-transform infrared spectroscopy. The antimicrobial activity of OrZnO and OrSe NPs was assessed employing the disc diffusion and agar well diffusion methods, while in vitro antioxidant activity was assessed through three different assays: DPPH, FRAP, and ABTS. The MTT assay was utilized to ascertain the cytotoxicity of these nanoparticles against lung cancer, and acridine orange/ethidium bromide staining was carried out to analyze morphological and DNA alterations in these cells. Consequently, the synthesized OrZnO NPs exhibited stronger antibacterial capabilities than the OrSe NPs, while both demonstrated antioxidant properties. In addition to their potent cytotoxic action, both NPs induced apoptosis by altering the DNA and shape of the lung cancer (A549) cells
Determination of Bioactive and Anti-Inflammatory Molecules of Thymbra Spicata L. from Mardin by GC-MS and LC-Orbitrap HRMS: A DFT, Molecular Docking, ADMET, Biological Target and Activity Study
No full textObjectives In this study, phenolic compounds (PC), volatile compounds (VC), antioxidant, anti-inflammatory, and antimicrobial properties of the Thymbra spicata L. plant grown in the Mardin Yesilli region were investigated. The properties of compounds such as rhoifolin, leucoside, naringenin, rosmarinic acid, gamma-terpinene, and carvacrol, which were found to be higher than other compounds as a result of PC and VC analysis of Thymbra spicata L. plant, and the properties of non-steroidal anti-inflammatory drugs diclofenac and ketoprofen were compared with computer-based calculations. In this context, the Absorption, Distribution, Metabolism, Excretion, and Toxicity (ADMET) profiles of the mentioned molecules were calculated, Density Functional Theory (DFT)-based quantum chemical calculations were performed, and molecular docking analyses were performed. In addition, the pharmacological properties, biological targets, and biological activities of these compounds were comprehensively evaluated. This study provides important data for understanding the therapeutic potential of these plant components. Materials and methods Chromatographic methods (GC-MS and LC-Orbitrap HRMS) were used to look at PC and VC profiles, spectrophotometry (ABTS, DPPH, CUPRAC) to look at antioxidant activities, and the disk diffusion method to look at antimicrobial properties. Results While the most detected volatile compounds were gamma-terpinene (35.34%), carvacrol (17.55%), caryophyllene (10.53%), and p-cymene (9.15%), the most detected phenolic compounds were rosmarinic acid (3334.9 ng/mL), naringenin (2610.00 ng/mL), leucoside (1956.42 ng/mL), and rhoifolin (1288.55 ng/mL). The ethanol extract of Thymbra spicata L. stopped the growth of K. aerogenes in particular. The IC50 value for DPPH was 158.01 mu g/mL, the IC50 value for ABTS was 185.03 mu g/mL, and the C0.5A value was 87.13 for the ethanol extract of Thymbra spicata L. The molecular docking results that the strongest binding energy was between TNF-alpha and naringenin (-6.77 kcal/mol), IL-6 and rosmarinic acid (-5.55 kcal/mol), and NLRP3 and carvacrol (-6.88 kcal/mol). Naringenin, rosmarinic acid, diclofenac, and ketoprofen completely violated the restrictions established by Lipinski, Ghose, Veber, Egan, and Muegge. Rosmarinic acid is the most reactive antioxidant compound with low energy deficit (Eg) and high softness (S). gamma-terpinene and carvacrol, on the other hand, are more stable and durable wit high hardness (eta) and low softness. Conclusion Naringenin, rosmarinic acid, and carvacrol demonstrate significant anti-inflammatory and immunomodulatory properties owing to their high binding affinity for TNF-alpha, IL-6, and NLRP3, positioning them as promising candidates for the treatment of inflammatory disorders. Naringenin and rosmarinic acid effectively target TNF-alpha, whereas carvacrol demonstrates potential in the management of NLRP3 inflammasome-associated diseases. The strong binding of rosmarinic acid to IL-6 shows that it can change the immune system. Thymbra spicata L., abundant in bioactive compounds, may provide benefits compared to conventional NSAIDs and exhibit potential as monotherapy or adjunctive therapies. Their strong anti-inflammatory, antioxidant, antimicrobial, and pharmacological properties, along with their safety, make naringenin, rosmarinic acid, and carvacrol good candidates for more preclinical and clinical research
Comparison of Chemical and Antimicrobial Properties of Different Nanoparticles Synthesized From Verbascum x Calcicolum Hub.-Mor. Hybrid
No full textErcan, Leyla/0000-0002-6570-8128;Recent advances in nanoparticle synthesis have led to an increase in the use of environmentally acceptable plantderived nanoparticles, given their wide range of applications. Due to their stability and several advantageous characteristics, selenium nanoparticles also stand out in these investigations in addition to metallic nanoparticles like silver, gold, iron, and zinc. These nanoparticles' favorable qualities for human health, such as antioxidant and antibacterial activities, are being studied in addition to their chemical characteristics. This investigation aimed to produce zinc oxide nanoparticles (VcZnO) and selenium nanoparticles (VcSe) from hybrid Verbascum x calcicolum Hub.-Mor. And to examine their antioxidant and antimicrobial properties. Utilizing the disk diffusion method and well methods, its antibacterial capabilities were studied. In vitro antioxidant capabilities were also assessed utilizing the DPPH, FRAP, and CUPRAC techniques. As a result, it emerged that zinc oxide nanoparticles synthesized from hybrid Verbascum x calcicolum were effective on gram-positive bacteria and gram-negative bacteria such as Staphylococcus aureus (MIC = 160 mg/ml for VcZnO), Pseudomonas aeruginosa (MIC = 80 mg/ml for VcZnO), Klebsiella pneumoniae (MIC = 40 mg/ml for VcZnO and, MIC = 120 mg/ml for VcSe), Escherichia coli (MIC = 20 mg/ml for VcZnO). While selenium nanoparticles are more favorable in terms of antioxidant capabilities, zinc nanoparticles demonstrate stronger antibacterial properties than selenium nanoparticles. Thus, it has been discovered that the use of different nanoparticles of various plants can be more efficient in different applications. It has also been found that Verbascum x calcicolum is a material that can be used successfully in nanoparticle synthesis
Comparison of Chemical and Antimicrobial Properties of Different Nanoparticles Synthesized From Verbascum X Calcicolum Hub.-Mor. Hybrid
No full textErcan, Leyla/0000-0002-6570-8128Recent advances in nanoparticle synthesis have led to an increase in the use of environmentally acceptable plantderived nanoparticles, given their wide range of applications. Due to their stability and several advantageous characteristics, selenium nanoparticles also stand out in these investigations in addition to metallic nanoparticles like silver, gold, iron, and zinc. These nanoparticles' favorable qualities for human health, such as antioxidant and antibacterial activities, are being studied in addition to their chemical characteristics. This investigation aimed to produce zinc oxide nanoparticles (VcZnO) and selenium nanoparticles (VcSe) from hybrid Verbascum x calcicolum Hub.-Mor. And to examine their antioxidant and antimicrobial properties. Utilizing the disk diffusion method and well methods, its antibacterial capabilities were studied. In vitro antioxidant capabilities were also assessed utilizing the DPPH, FRAP, and CUPRAC techniques. As a result, it emerged that zinc oxide nanoparticles synthesized from hybrid Verbascum x calcicolum were effective on gram-positive bacteria and gram-negative bacteria such as Staphylococcus aureus (MIC = 160 mg/ml for VcZnO), Pseudomonas aeruginosa (MIC = 80 mg/ml for VcZnO), Klebsiella pneumoniae (MIC = 40 mg/ml for VcZnO and, MIC = 120 mg/ml for VcSe), Escherichia coli (MIC = 20 mg/ml for VcZnO). While selenium nanoparticles are more favorable in terms of antioxidant capabilities, zinc nanoparticles demonstrate stronger antibacterial properties than selenium nanoparticles. Thus, it has been discovered that the use of different nanoparticles of various plants can be more efficient in different applications. It has also been found that Verbascum x calcicolum is a material that can be used successfully in nanoparticle synthesis
Phytochemical Profile, Antimicrobial, Antioxidant, Anti-Xanthine Oxidase, and Anti-Elastase Activities of Centaurea Hyalolepis: an in Silico and in Vitro Analysis
No full textAkan, Hasan/0000-0002-3033-4349; Ercan, Leyla/0000-0002-6570-8128This study aimed to examine the antibacterial, antioxidant, and enzyme activity of Centaurea hyalolepis, as well as the total phenolic and carotenoid contents, and volatile and phenolic components. Gas chromatography-mass spectrometry was used for the volatile component analysis of C. hyalolepis, while liquid chromatography-mass spectrometry was used for the phenolic component analysis. Furthermore, the antimicrobial activity was evaluated using disc diffusion and microdilution techniques. The antioxidant capacity was investigated in vitro using four distinct methods (2,2-diphenyl-1-picrylhydrazyl, 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid, cupric ion-reducing antioxidant capacity, and N,N-dimethyl-p-phenylene diamine). Additionally, both in vitro and in silico studies were carried out to determine the inhibitory effect on xanthine oxidase and elastase. Consequently, it was determined that C. hyalolepis has antioxidant properties and is a highly efficient antibacterial agent against Bacillus megaterium, Pseudomonas aeruginosa, Bacillus subtilis subsp. spizizenii, Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus, Klebsiella aerogenes, and Candida albicans. Additionally, C. hyalolepis contains many volatile compounds, such as 2-methyl propanoic acid, 3,5-octadien-2-one, 2,6-dimethyl cyclohexanol, hexanal, pentanal, anethole, and beta-cyclocitral. It also has a large number of phenolic components, including chlorogenic acid, apigenin 7-glucuronide, quinic acid, and 4-hydroxybenzoic acid. Moreover, C. hyalolepis was shown to inhibit xanthine oxidase and elastase
Comparison of Antioxidant and Antimicrobial Properties of Zinc Oxide and Selenium Oxide Nanoparticles Using Verbascum Kotschyi Boiss. & Hohen
No full textErcan, Leyla/0000-0002-6570-8128; Mungan Kilic, Fatma/0000-0001-6858-3458Nanoparticle applications have been studied in many fields in recent years. Among these studies, the synthesis of nature-friendly and healthfriendly nanoparticles through green synthesis attracts much attention. These investigations also highlight the significance of several plant species, many of whose worth and traits remain unknown. The goal of this work is to create zinc oxide and selenium nanoparticles from Verbascum kotschyi Boiss. & Hohen., a plant species that hasn't received much attention, and to ascertain the antioxidant and antibacterial qualities of these nanoparticles. To accomplish this, three distinct techniques (DPPH, CUPRAC, and FRAP) were used to assess the produced nanoparticles' in vitro antioxidant capabilities after SEM, EDX, and FTIR analyses. Furthermore, the disk diffusion technique was utilized to ascertain the antibacterial efficacy of these nanoparticles against both gram-positive and gram-negative bacteria and fungus. In conclusion, V. kotschyi-derived zinc oxide nanoparticles outperformed selenium nanoparticles in terms of antibacterial activity. But when it came to antioxidant activity, selenium nanoparticles outperformed zinc oxide nanoparticles. Thus, it was determined that the products created by nanoparticle synthesis from Verbascum kotschyi have properties that can be used in different fields
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
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